Abstracts of the Annual Meeting of the Iuliu Haţieganu, University of Medicine and Pharmacy : 2nd – 6th December 2019, Cluj-Napoca

Sevastre-Berghian A, Hanganu D, Oniga I, Ciocan A, Nadim A, Horon B, Mogoşan E, Cazacu I, Cristina A, Voştinaru O, Anca E, Lang C, Roşioru C, Erhan S, Vlad C, Capraş R, Urda-Cîmpean A, Perde-Schrepler M, Bindea M, Tatomir C, Ujvary P, Maniu A, Schiotis R, Radu C, Goşa D, Domşa E, Filip G, Olteanu D, Baldea I, Clichici S, Mureşan A, David L, Moldovan B, Para I, Andreica V, Neag M, Cătinean A, Pop M, Todea M, Pop-Mureşan M, Vulpoi A, Popa C, Berce P, Eniu D, Sabin O, Chedea V, Boca A, Sitar-Taut A, Fodor A, Tarmure S, Sampelean D, Grosu A, Grosu L, Crisan S, Budin C, Alexescu T, Răchişan A, Dubois V, Ranchin B, Leclerc A, Thomas A, Cochat P, Bacchetta J, Buzdugan E, Condor A, Crişan S, Rădulescu D, Barta A, Mocan M, Anton F, Mocan-Hognogi D, Goidescu C, Farcaş A, Lazea C, Al-Khzouz C, Bucerzan S, Asavoaie C, Nascu I, Grigorescu-Sido P, Jianu C, Itu-Muresan C, Topan A, Filipescu I, Jianu M, Bolboaca S, Cîmpianu M, Blachon A, Chiorescu R, Stoia M, McPherson C, Pop S, Ruskin J, Blendea D, Benţa G, Căinap S, Sîrbe C, Miclea D, Pîrvan A, Platon M, Ciulei G, Orăşan O, Procopciuc L, Burac L, Aldea C, Delean D, Bizo A, Bulata B, Fufezan O, Stefanescu H, Hotea I, Crisan T, Pamfil C, Badii M, Gaal O, Peca L, Mirea A, Popp R, Joosten L, Cismaru G, Mocanu L, Gheorghiu C, Simu G, Rosu R, Puiu M, Gusetu G, Caloian B, Zdrenghea D, Perné M, Coman O, Hăşmăşanu M, Matyas M, Zaharie G, (Tamaş) A, Ciule D, Maierean A, Todea D, Vele P, Silaghi C, Simon S, Craciun A, Rednic S, Blaga L, Muresan R, Tomescu S, Chis A, Rednic M, Lazăr M, Sas V, Deleanu C, Man S, Schnell C, Nicolescu A, Vulturar R, Vinţan M, Poliac T, Chiş A, Damian L, Maghea L, Guţiu R, Suroiu S, Grama A, Sirbe C, Borodi M, Benta G, Pirvan A, ANDREI A, Pop T, Coste S, Cozma A, Orasan R, Orasan O, Şerban O, Papp I, Bocşan C, Micu M, Bădărînză M, Albu A, Fodor D, Slavescu K, Slavescu R, Mititelu A, Radulescu D, Dhot J, Ţaranu I, Răcătăianu N, Milaciu M, Bocşan I, Ciumărnean L, Sâmpelean D, Negrean V, Pop R, Matei D, Paşca S, Buzoianu A, Acalovschi M, Borz P, Florian T, Florian I, Olteanu G, Radu M, Tui I, Andercou O, Stancu B, Gherman C, Constantinescu I, Mihaileanu F, Pintea D, Puşcaş M, Mocan-Hognogi R, Dudea M, Fetica B, Clim A, Nechita V, Bolboacă S, Graur F, Moiş E, Hajjar N, Măluţan A, Suciu V, Ignat F, Ciortea R, Boţan E, Bucuri C, Rada M, Mihu D, Lăcan S, Ungur O, Golea A, Ujvary L, Blebea C, Bulac L, Birta D, Chirilă M, Munteanu V, Borz M, Cojocaru I, Marica N, Popescu D, Schitcu V, Ciocan R, Crişan D, Constantinescu D, Muntean V, Cozma-Petruţ A, Badiu-Tişa I, Filip L, Banc R, Stanciu O, Gavrilaş L, Ciobârcă D, Hegheş C, Miere D, Brém B, Tötös R, Coman F, Leonte D, Toma A, Casoni D, Zaharia V, Melinte G, Cernat A, Irimes M, Györfi S, Suciu M, Anicăi L, Cogălniceanu G, Mitoi M, Pralea I, Buse M, Ionescu C, Pricopie A, Ionuţ I, Găină L, Vodnar D, Focşan M, Rus I, Tertiş M, Gug I, Paşcalău V, Melean B, Pall E, Săndulescu R, Cristea C, Iacob B, Toma V, Bodoki E, Oprean R, Marc G, Stana A, Pîrnău A, Oniga O, Moldovan C, Gavan A, Dias M, Pereira C, Barros L, Ferreira I, Crişan G, Negoi O, Ispas G, Muşat L, Muntean A, Casian T, Barbălată C, Cherfan A, Hegheş S, Petrache A, Ardelean A, Moisescu C, Negut D, Ardelean I, Crişan A, Ilyés K, Porfire A, Stoicescu L, Ştefan G, Lauzier B, Morgovan C, Ghibu S, Herda L, Nicoară R, Ilieş M, Giudice M, Polo E, Dawson K, (Savencu) I, Corzo C, Tomuţă I, Salar-Behzadi S, Fizeşan I, Iacoviţă C, Topală T, Scoruş L, Lucaciu C, Rus L, Kacso I, Pari S, Valica V, Uncu L, Iuga C, Ştefan M, Pop C, Bâlteanu V, Mogoşan C, Ghibu-Morgovan S, Dinte E, Benedec D, Miclaus V, Rusu M, Fizesan I, Gheldiu A, Stefan M, Mates L, Moldovan R, Muntean D, Pop A, Georgiu C, Mocan A, Vostinaru O, Kiss B, Loghin F, Popa D, Oroian M, Pop D, Marcovici A, Bhardwaj S, Vlase L, Rotar A, Băbţan A, Vesa Ş, Boşca B, Crişan M, Petrescu N, Feurdean C, Uriciuc W, Ionel A, Buhăţel D, Sava A, Andrei V, Câmpian R, Tişler C, Negucioiu M, Duncea I, Berar A, Picoş A, Ostas D, Stan H, Florian A, Florian S, Armencea G, Onisor F, Moldovan (Lazar) M, Vacaras S, Muresan O, Baciut M, Baciut G, Rotar H, Crasnean E, Coclici A, Cristian D, Micu I, Ţermure D, Ciurea A, Soancă A, Roman A, Chifor I, (Dascalu) L, Picos A, Chifor R, Badea I, Simu M, Badea M, Lazar A, Ilea A, Bel L, Sarpatoczi O, Lacatus R, Taulescu M, Rusnac M, Gasparik C, Varvara B, Dudea D, Colceriu-Şimon I, Ştiufiuc R, Aghiorghiesei A, Ţărmure V, Hedeşiu M, Mirica I, Furtos G, Fontaine V, Lucaciu O, Campian R, Sbuciumelea I, Ioani C, Părăian A, Hedesiu M, Tamaş T, Dinu C, Stoia S, Băciuţ G, Bran S, Mitre I, Rotaru H, Băciuţ M, Crişan L, Crişan B, Onişor F, Roman R, Mureşan O, Moldovan M, Ostaş D, Manea A, Almăşan H
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Wild and cultured Thymus marshallianus extracts reduced oxidative stress markers and DNA damage in hyperglycemic endothelial cells, suggesting potential benefits for diabetes-related complications.

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This study evaluated the comparative effects of wild and cultured Thymus marshallianus extracts on human umbilical vein endothelial cells under hyperglycemic conditions. The researchers characterized the phytochemical profiles using HPLC-MS/MS and assessed oxidative stress markers, including MDA levels and NF-kB activation via western blotting. Results showed that both extracts reduced DNA damage and inflammatory markers while enhancing antioxidant defenses, suggesting potential benefits for diabetes-related complications. The paper does not explicitly discuss endometriosis or adenomyosis; it was included in the corpus via a keyword match in the upstream search index.

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Intro

Polyphenolic compounds administration may prevent neurodegeneration, inhibit inflammations and reduce age – related cognitive decline by scavenging free radicals, activating various signaling pathways (NFkB) or inhibiting the release of cytokines (TNF-α). We aimed to chemical characterize and evaluate the comparative effects of two extracts of Thymus marshallianus L. (TM), from wild flora (TMW) and from culture (TMC) on in vitro induced hyperglycemia.

Results

Rosmarinic acid (RA) was the phenolic marker with the highest level determined in both TMW and TMC extracts. TMW extract contained higher amounts of ferulic acid, apigenin and kaempferol, while luteolin amount was the highest in TMC extract. Catechin and protocatechuic acid were found in TMC extract. In vitro, TMW and TMC extracts diminished MDA, NF-kB and γH2AX levels and increased pNF-kB and HIF 1α expressions.

Conclusions

Our findings indicate that TM extracts administration might represent a good option in diabetes – related complications. Both compounds exerted beneficial effect by increasing the antioxidant defence, survival molecules and diminishing DNA lesions.

Materials|Methods

The phytochemical profile of lyophilized extracts was qualitatively and quantitatively analyzed by two HPLC-MS/MS methods, assessing the individual polyphenolic compounds. In vitro studies were performed on HUVEC cell line, using TMW and TMC extracts in two different dilutions (1/10000, 1/100000), under normoglycemic and hyperglycemic conditions. NF-kB, activated pNF-kB, HIF 1α and γH2AX were assessed by western blot; MDA levels were analyzed by spectrofluorimetry.

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