Controlling the morphologies and dynamics in three-dimensional tissues

Controlling the morphologies and dynamics in three-dimensional tissues | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Controlling the morphologies and dynamics in three-dimensional tissues Devarajan (Dave) Thirumalai, Rajsekhar Das, Xin Li, Sumit Sinha This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6993695/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted You are reading this latest preprint version Abstract A number of factors, such as, cell-cell interactions and self-propulsion of cells driven by cytoskeletal forces determine tissue morphologies and dynamics. To explore the interplay between these factors in controlling the dynamics at the tissue scale, we created a minimal three dimensional model in which short-range repulsive elastic forces account for cell-cell interactions. Self-propulsion is modeled as active uncorrelated random stochastic forces, with strength μ, that act on individual cells and is the only source of cell motility. Strikingly, variations in polydispersity in cell sizes (Σ) and cell elasticity (E), results in the formation of a variety of distinct ``phases", driven entirely by μ. At low E, the tissue behaves like a liquid, at all values of Σ, whereas at high E and Σ, it has the characteristics of a glass. The tissue crystallizes at low Σ provided E exceeds a critical value. Over a narrow range of E and Σ, that lies between the boundaries of the liquid and glass phase, the effective viscosity increases like in a glass as the cell density increases and saturates as the cells are compressed beyond a certain value, creating the viscosity saturation (VS) phase. The VS phase does not form in systems at finite temperature in which the dynamics satisfies the Fluctuation Dissipation Theorem. In the glass phase, the tissue exhibits aging (relaxation times depend on the waiting time) behavior at high E values. Our findings provide a framework for designing tissues with tunable material properties by controlling the physical characteristics of cells. Biological sciences/Biophysics Physical sciences/Physics/Condensed-matter physics Full Text Additional Declarations There is NO Competing Interest. Supplementary Files ChangingCellElasticitySI.pdf Manuscript Supplementary Informations VideoCaptions1.pdf Captions of Videos Video1.mp4 Video1 Video2.mp4 Video2 Video3.mp4 Video3 Cite Share Download PDF Status: Under Review Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. 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