Shedding suppresses receptor-ligand trogocytosis to enhances long-term antitumor potential

Shedding suppresses receptor-ligand trogocytosis to enhances long-term antitumor potential | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Shedding suppresses receptor-ligand trogocytosis to enhances long-term antitumor potential Atsutaka Minagawa, Tomoko Ishi, Shin Kaneko This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5274199/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Trogocytosis is a curious phenomenon in which the rapid transfer of cell-membrane proteins between cells is induced by receptor-antigen ligation. In Chimeric antigen receptors therapy, the target antigen is transferred to T cells via trogocytosis, thereby decreasing the target density on tumor cells and abating the T cell activity. The result is tumor immune escape and T cell fratricide, two factors known to cause relapse after Chimeric antigen receptors therapy. Here we report receptor shedding as a mechanism to suppress trogocytosis. The shedding structure of Chimeric antigen receptors corresponded with the trogocytosis and long-term antitumor effect. This link between trogocytosis and shedding indicates a previously unknown form of communication between proximal cells. Health sciences/Oncology/Cancer/Cancer therapy/Cancer immunotherapy Biological sciences/Cell biology/Membrane trafficking/Endocytosis Full Text Additional Declarations Yes there is potential Competing Interest. Shin Kaneko is the founder, shareholder, and director of Shinobi Therapeutics, Inc., and has received research funding from Shinobi Therapeutics, Inc., Takeda Pharmaceutical Co., Ltd., Astellas Co., Ltd., Terumo Co., Ltd., Mitsui-soko Co.,Ltd., and KOTAI Biotechnologies, Co., Ltd. Atsutaka Minagawa and Shin Kaneko have a patent registration related to this work. Supplementary Files supmovie1.mov Supplemental movie 1 supmovie2.mov Supplemental movie 2 nreditorialpolicychecklistMinagawa.pdf Editorial policy checklist Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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