Effectiveness of Proprioceptive Neuromuscular Facilitation Pattern on Upper Extremity and Scapula in Patients with Adhesive Capsulitis: A single centre assessor-blinded Randomised controlled trial (RCT)

Effectiveness of Proprioceptive Neuromuscular Facilitation Pattern on Upper Extremity and Scapula in Patients with Adhesive Capsulitis: A single centre assessor-blinded Randomised controlled trial (RCT) | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Effectiveness of Proprioceptive Neuromuscular Facilitation Pattern on Upper Extremity and Scapula in Patients with Adhesive Capsulitis: A single centre assessor-blinded Randomised controlled trial (RCT) Abid Hasan Khan, Md. Saruar Hossain Bhuiyan, Md. Feroz Kabir, and 4 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4843786/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background Adhesive capsulitis (AC) is a progressive inflammatory condition of the shoulder that causes functional limitations and leads to long-term disability. The study aimed to elicit the effectiveness of Proprioceptive neuromuscular facilitation (PNF) compared to standard physiotherapy approaches on AC. Methods An assessor-blinded single-centre Randomized control trial (RCT) was carried out on 80 AC patients between May and December 2023 in Bangladesh. Random assigned and concealed allocated patients were recruited equally (n = 40) to each PNF and conventional capsular stretching group. The experimental group received a PNF approach, and the control group received capsular stretching to the affected shoulder for 24 sessions in 6 weeks. Both groups received electrical modalities as standard treatment. The primary outcome was pain measured by the Numeric pain rating scale (NPRS) and range of motion in a universal goniometer. The secondary outcome was functional limitation measured by the Shoulder pain and disability index (SPADI). As per the distribution of data, non-parametric tests were employed to analyse the superiority between and within groups with intention-to-treat analysis. Results Baseline compatibility was noted in all the key variables (P > 0.05). Both PNF and capsular stretching had a decrease in pain compared to baseline in all capsular pattern positions (P < 0.001), and PNF had more significant improvement compared to control (P < 0.001). Both groups had equal improvement in shoulder ROM (P < 0.001) except abduction (P < 0.05). Both groups had improvements in disability (P < 0.05), and PNF had statistical superiority of improvement (P < 0.001). Conclusions The findings of this study support the potential of PNF intervention for 6 weeks as a treatment for shoulder Adhesive capsulitis, showing improvements in pain, ROM, and functional disability. However, further multicentre trials with a follow-up design are needed to fully understand the superiority of PNF on shoulder AC, encouraging continued engagement in this area of research. Trial registration: The Australian New Zealand Clinical Trial Registry ( http://www.anzctr.org.au ) (ACTRN12621001299897). Adhesive Capsulitis Proprioceptive Neuromuscular Facilitation (PNF) Pattern Upper Extremity Standard Physiotherapy Figures Figure 1 Figure 2 Figure 3 Background Adhesive capsulitis (AC) is a musculoskeletal disorder characterized by painful limitation of upper limb activities that is associated with a combination of pain and limitation of passive movements [ 1 ]. The joint capsule of the shoulder becomes inflamed and gradual limitation of shoulder range of motion occurs; the condition is often explained as a stiff shoulder [ 2 ]. Aetiology is unknown but cardiovascular disease, cervical spine conditions, diabetes, sudden trauma, stroke, Parkinson's disease, Coronary artery bypass grafting (CABG) and humeral fractures are often associated with AC [ 3 ]. Moreover, some intrinsic factors such as injuries to the rotator cuff muscles, long head of the biceps tendon, joint inflammation and calcification of the biceps tendon and other tendons may contribute to AC [ 4 ]. AC is believed to be a self-limiting condition lasting two to three years, some studies have reported that up to 40% of patients have persistent symptoms and stiffness even after three years because pain and inflammation are self-remitting but the muscle atrophy and joint pathology are consistent if not treated [ 5 ]. It is difficult to find an actual population prevalence of AC, the incidence is evident between 3% and 5% of the normal population in a given year [ 6 , 7 ]. AC greatly affects between forty to sixty years of aged people, with females being higher than males [ 6 ]. About 20 to 30 percent of people having AC in one shoulder develop symptoms in the opposite shoulder after a certain period [ 7 ]. The biomechanical abnormalities of the scapulohumeral rhythm and muscle control of the prime movers of the shoulder especially the deltoid and rotator cuff play vital role in developing AC associated musculoskeletal issues [ 8 ]. Moreover, the painful movement contributes to kinesiophobia that gradually hinders the flexibility of the muscles of shoulder and causes disruption to the scapula-humeral synergistic relationship [ 9 ]. The medical treatment for AC involves the use of nonsteroidal anti-inflammatory medications, short-term oral corticosteroids, intra-articular corticosteroid injections, physiotherapy, acupuncture, and hydro-dilatation [ 10 ]. The role of physiotherapy is to facilitate the arthrokinematic motion of glenohumeral joint, acromio-clavicular joint and improve the osteokinemetics of these joints including scapula-thoracic articulation [ 11 ]. The gradual mobility and motion facilitate a range of motion (ROM), mimic the inflammatory mediators, and break down the scars of the joint capsule [ 12 ]. The convex-concave rule explains the necessity of neuro-muscular flexibility in improving the movement limitations in capsular patterns [ 13 , 32 ]. There are a couple of concepts in the management of AC; Proprioceptive neuromuscular facilitation (PNF) is one of them [ 14 ]. PNF has three theoretical mechanisms, autogenous inhibition, behaviour modification, and stress relaxation [ 15 ]. The gate control theory of PNF is explained to enhance ROM that facilitates muscle activation [ 16 ]. Evidence suggests PNF treatment increases blood flow by up to 71% in the administered muscles and eases subjective pain by up to 16% from baseline [ 14 ]. PNF added with conventional stretching and mobilization can decrease subjective pain by up to 21% [ 17 ]. In AC, PNF effects on stretch reflex mechanism and facilitates muscles by utilizing diagonal movement patterns to activate the bi-articular muscles of the shoulder [ 18 ]. PNF is a promising treatment among therapeutic approaches to improve pain, muscle flexibility, and increase ROM [ 17 ]. An all-encompassing strategy for AC management necessitates methods to enhance the effective functioning of the upper limbs, taking into account both the movement and stability of the scapula. The scapula pattern of Proprioceptive Neuromuscular Facilitation (PNF) simultaneously engages both upper extremity movement patterns and scapular motions [ 19 ]. The effects of PNF on the shoulder have been investigated in some studies [ 5 , 20 ]. Nevertheless, there is a lack of research that explores the impact of upper extremity and scapular proprioceptive neuromuscular facilitation (PNF) exercises in the rehabilitation of the acromioclavicular (AC) joint. Hence, the objective of this study was to assess the efficacy of the Proprioceptive neuromuscular facilitation pattern (PNF) in treating adhesive capsulitis by comparing its efficiency on the upper extremity and scapula with traditional physiotherapy. Our hypothesis was PNF alone might have a superior effect compared to conventional exercise therapy in improving pain, muscle flexibility, ROM, shoulder function and disability in Adhesive capsulitis (AC). Methods Study Design An accessor-blinded Randomised controlled trial (RCT) was conducted in the outpatient Physiotherapy musculoskeletal unit at the Department of Physiotherapy at the Centre for the Rehabilitation of the Paralysed (CRP), Savar, Dhaka- 1343, Bangladesh between May and December 2023. The population of interest was people having adhesive capsulitis diagnosis in hospital records. Ethical approval was received from the Institutional Review Board of Bangladesh Health Professions Institute (BHPI), and trial registration was obtained prospectively from the Australian New Zealand Clinical Trial registry platform (ACTRN12621001299897) (Date: 27/09/2021) [Annexure 1]. Recruitment of Patients and Randomization The names and contact details of patients who had adhesive capsulitis were retrieved from hospital records of outpatient department software named Patient Data Management System (PDMS) at CRP. They were population frames. Then, patients were then contacted via a telephone call to invite them to the study. Patients interested in the study were screened against the inclusion/exclusion criteria using a telephone-administered questionnaire. The inclusion criteria were (1) adults aged between 21 and 70 years [ 21 ], (2) re-diagnosis as unilateral adhesive capsulitis (AC) by a qualified physiotherapist according to the Cyriax concept capsular pattern theory and (3) patient with shoulder pain at least one month [ 5 ]. Patients were excluded if they reported (1) a history of surgery on the shoulder or manipulation under anaesthesia, (2) neurologic deficits affecting shoulder functioning during ADL or diagnosed as brachial plexopathy by nerve conduction study (NCS) (3) pain or disorders of the cervical spine, elbow, wrist, or hand and (4) other conditions involving the shoulder as rotator cuff tear, or tendinitis etc. [ 5 ]. Eligible participants were referred to the musculoskeletal unit one and two of the Department of Physiotherapy, CRP for further screening with a concealed random allocation after the screening process. Before going for the treatment in every unit, there was a separate cubicle for the blinded assessor to complete the informed consent and baseline assessment. After the end of the treatment protocol, each patient re-entered the same room for outcome evaluation. A computerised random allocation technique using Microsoft Excel 2013 was used to assign the participants in both groups. The Consolidated Standards of Reporting Trial (CONSORT) was supplied in Fig. 1. The sample size was estimated using statistical power analysis processes with the assistance of the PASS 2005 program (NCSS, Kaysville, UT, USA). Based on a power analysis, it was determined that each group would require 30 individuals in order to achieve a power of 90% and maintain a type I error rate of 5%. To account for a dropout rate of 20% in the trial, we enrolled 40 participants in each group to guarantee a power of 90%. The power analysis conducted for our study revealed a statistical power of 90%, with adhesive capsulitis being the key outcome measure. Figure 1 CONSORT flow diagram Outcome Measurement Numerical pain rating scale As the primary outcome, we used the numeric pain rating scale (NPRS). The NPRS is a linear scale where the individual being evaluated indicates the intensity of their pain by marking a point on the line. The endpoints of the linear scale correspond to the farthest thresholds of pain, where 0 indicates the absence of pain, 1–3 denotes mild discomfort, 4–6 signifies moderate pain, and 7–10 represents the most excruciating agony ever encountered [ 22 ]. Goniometer To measure another primary outcome goniometer was used to measure ROM. We assessed the range of motion at the initial assessment and post-test assessment with the same goniometer to ensure consistency. A goniometer is a valid and reliable tool for measuring ROM globally [ 23 ]. Shoulder pain and disability index (SPADI) Additionally, we assessed function, pain level, and disability using the SPADI scale as a supplementary measure. The system is composed of two sections, one dedicated to pain-inducing activities and the other focused on functional tasks, the pain dimension comprises 5 inquiries regarding the intensity of an individual's pain. The assessment of functional activities involved the use of eight specific questions aimed at determining the level of difficulty that individuals experience in performing various daily tasks that require the use of their upper limbs. The SPADI can be completed by a patient in a time frame of five to ten minutes and is the only dependable and accurate measure specifically designed for assessing the shoulder [ 24 ]. Intervention Experimental group Within this group, we employed the Proprioceptive Neuromuscular Facilitation Technique (PNF) specifically for the upper extremity [ 21 ]. The patient was resting on their back with their arms positioned alongside their torso. The individuals were instructed to loosen the tension in their shoulder muscles. The therapist positioned themselves next to the impacted side of the patient. The technique used was the "Flexion-abduction-external rotation" (D2F pattern) with a straight elbow pattern of proprioceptive neuromuscular facilitation (PNF) using the "hold relax" technique. This equipment is designed to provide relaxation by using isometric contractions against maximum resistance. It is intended to enhance the passive range of motion and reduce pain. The individual executed isometric contractions for 5–8 seconds, applying maximal resistance. These contractions were specifically targeted to prevent the opposing muscles from contracting and included a rotation at the point of maximum joint movement. The technique was done multiple times until reaching the point of movement restrictions and then advanced to additional stages. When implementing the D2F pattern with a straight elbow pattern of PNF, the therapists used their hands to grasp the patient's upper limb on the side opposite to the hip. The patient's shoulder was positioned in extension, adduction, and internal rotation, while the elbow was extended and the forearm was pronated. The physiotherapist thereafter instructs the patient to elevate his hand above his head. The patient endeavoured to execute this motion by engaging in shoulder flexion, abduction, and external rotation. During these movements, the therapist would stabilize the patient's arm with his other hand (Fig. 2). The duration of treatment was 15 minutes for every 10 sets of PNF. There were 5 repetitions per set, with 5-second hold time, and 2 seconds of rest time. There was no progression of sets. Treatment was provided for 6 weeks and 4 sessions per week in alternative days. The second technique was the Proprioceptive Neuromuscular Facilitation Technique for the Scapula and it has two patterns (Fig. 2). Firstly anterior elevation & posterior depression. The patient reclines on the unaffected side, while the therapist positions themselves behind the patient, with one hand resting on the upper edge of the scapula and the other on the lower corner of the scapula. The therapist provided the patient with manual resistance and directed them to perform scapular movements by pushing up and down. The duration of treatment was 7 minutes for 5 sets. Treatment repetitions were 5 repetitions/set, with hold time for 5 second s , and rest time for 2 seconds. The treatment was given for a total of 6 weeks, 4 sessions per week. Additionally, in cases of posterior elevation and anterior depression, the patient is positioned on the side that is not affected. The therapist stood behind the patient, with one hand positioned on the superior border of the scapula and the other on the inferior angle of the scapula. The patient received instructions to exert force in a backward direction and apply pressure to the front of the scapula while the therapist provided resistance. The duration of treatment was 8 minutes per 5 sets. Repetitions were 5 repetitions/set, hold time for 5 seconds, and rest time for 2 seconds. The treatment was provided for a total of 6 weeks, 4 sessions per week. Figure 2 PNF Application Procedure for Upper Extremity (A1-A3) and Scapula (B1, B2) Control group The control group was given conventional physiotherapy only according to the patient’s response to treatment. Both groups underwent a standard intervention program consisting of conventional physiotherapy techniques, such as Capsular stretching, Accessory movements, Pendulum exercise, Pulley exercise, Infrared radiation applied to the affected shoulder for 15 minutes, and Ultrasound therapy tailored to the patient's condition and prescribed frequency. The control group received 30 minutes of treatment per session for 4 times a week for up to 6 weeks. The control treatment was according to the standard physiotherapy exercise protocol set by the outpatient musculoskeletal unit, Department of Physiotherapy, CRP, Savar, Dhaka-1343, Bangladesh. Statistical Analysis A parametric test was used to analyse interval or ratio data and a non-parametric test was used to analyse the nominal/ordinal data. The normality of data was checked by the Kolmogorov-Smirnov test. The value of the Kolmogorov-Smirnov test is less than 0.05, which indicates that the data distribution was not normal [ 25 ]. So, descriptive statistics like median and interquartile range (IQR) were used to analyse the sociodemographic information where the between-group mean difference analysis of pain intensity, improvement of ROM of different movements of shoulder and improvement of functional performance of the participants was analysed by Mann-Whitney U-test and the within-group analysis of pain intensity, improvement of ROM of different movements of shoulder and improvement of functional performance of the participants was done by Wilcoxon signed rank test. The alpha value was set as p < 0.05. Results Demographic and clinical characteristics The sociodemographic and clinical information are summarised in Table 1. The median age of the participants in the experimental group was 48 (43.25 to 60) and in the control group 51 (45 to 57) years. In both groups, the male participants number was the maximum, 52.5% (n = 21) in the experimental group and 62.5% (n = 25) in the control group. Regarding educational status, most participants 32.5% (n = 13) completed SSC in the experimental group whereas 37.5% (n = 15) completed primary education in the control group. Most of the participants in the experimental group lived in the rural areas 47.5% (n = 19) but in the control group, they were mostly from urban areas, 42.5% (n = 17). Except for housewives, most of the participants were service holders in both groups, 27.5% (n = 11) in the experimental group and 25.0% (n = 10) in the control group. Regarding clinical characteristics, most of the participants had direct trauma in both groups, 60.0% (n = 24) in the experimental group and 62.5% (n = 25) in the control group. Hypertension was the most common chronic illness in both groups, 47.5% (n = 19) in the experimental group and 45.0% (n = 18) in the control group. Table 1 Demographic and clinical characteristics of participants at baseline Pain severity Both group participants were screened for their pain severity in the shoulder joint (Table 2). Based on some common activities patients were evaluated for pain in resting position where the within-group analysis showed significant change was found in both experimental group and control group (p < 0.001). In the case of pain during rising arm sideways, within-group analysis showed significant change was found in both the experimental group and control group (p < 0.001) and for pain during combing hair, within-group analysis showed significant change was found in both experimental group and control group (p < 0.001). For between-group analyses, significant changes were found in all three activities of the shoulder joint (p < 0.001). Table 2 Baseline and after-treatment status of pain severity by Numeric Pain Rating Scale (NPRS) Range of Motion (ROM) Limitation in shoulder joint was checked for only three movements shoulder abduction, lateral rotation and medial rotation. A significant median difference was observed after providing treatment between control and experimental group participants in terms of shoulder lateral and medial rotation movement (p 0.5). (Fig. 3) Figure 3 Evaluation of median difference in the ROM of shoulder movements after treatment Shoulder pain and disability index Participants were screened by Shoulder pain and disability index at baseline and after completing the whole treatment sessions (Table 3). In the case of pain intensity, within-group analysis showed significant change was found in both the experimental group (p < 0.001) and control group (p < 0.05). In the case of disability level, within-group analysis showed significant change was found in both the experimental group (p < 0.001) and control group (p < 0.05) and for total SPADI score, within-group analysis showed significant change was found in both experimental group (p < 0.001) and control group (p = 0.021). For between-group analyses, significant changes were found in all parameters of SPADI (p < 0.001). Table 3 Baseline and after-treatment scores for pain, disability and total SPADI score by using Shoulder Pain and Disability Index (SPADI) Discussion This study was carried out to determine the effect of PNF compared to standard physiotherapy in adhesive capsulitis in a Bangladeshi setting. The primary objective was to determine the superiority of PNF in relieving pain, improving ROM and improving the functional and disability status of AC. The objective was successfully examined with statistically significant superiority results in the PNF group in all indicators. In consideration, it was revealed that the mean age of the participants was 50 (44.25 to 60) years and among men 21.3% were government and non-government service holders and among women 42.5% were housewives. As for major working positions, 66.3% worked in a sitting position and 21.3% worked in a standing position. Among the participants, 55% had a history of overuse injury. These incidences are indicating the relationship between age and working characteristics with adhesive capsulitis. A randomized controlled study conducted on 36 participants by Akbas and colleagues [ 21 ] revealed that the mean age of the participants was 53.94 ± 9.38 and only 11 of 36 patients (30.6%) with adhesive capsulitis were still working in various jobs and sectors actively, rest of them weren’t working (15 retired − 10 unemployed) as of 69.4%. This study demonstrated the efficacy of scapular proprioceptive neuromuscular facilitation (PNF) exercises in reducing pain intensity during rest, arm abduction, and hair combing. Additionally, the study revealed that the PNF group had greater improvement in shoulder range of motion compared to the control group. However, the distinction was not statistically significant for abduction movements. Nevertheless, it was shown to be statistically significant for external and internal rotation movements. Additionally, significant improvements were observed in functional performance, such as discomfort and handicap status. In a randomized controlled study conducted by Lin and colleagues [ 20 ], it was found that both the scapular proprioceptive neuromuscular facilitation (PNF) group and the conventional physiotherapy group showed significant improvement. However, the PNF group had a greater reduction in pain and disability compared to the conventional group, as indicated by the mean value. It was elicited that, upper extremity and scapula PNF patterns provide additional benefit in only abduction movement of the shoulder, although do not provide benefit in resting pain, pain in an activity like raising the arm sideways or combing hair, internal and external rotation movement parameters in management of adhesive capsulitis. The within-group analysis of the experimental group revealed a significant reduction in pain at a statistically significant level of p < 0.001. The underlying idea here is that PNF (Proprioceptive Neuromuscular Facilitation) has been scientifically demonstrated to generate a pain-relieving effect by means of a gate control mechanism. The PNF approach applies pressure and proprioceptive inputs that reach the spinal level, effectively inhibiting the entry and transmission of pain signals [ 26 ]. In this study, there is a significant improvement in shoulder movement especially in internal rotation and external rotation found in the post-interventional increase of Range of motion in the PNF group. This can be occurred due to an increment in excitability and a decrease in response time. In their randomized study, Lee and colleagues [ 27 ] found that the combination of general physiotherapy techniques (including a 20-minute hot pack, 5 minutes of US therapy, and 20 minutes of TENS) was effective in improving pain and features associated with myofascial pain syndrome. They observed this positive outcome in approximately 32 participants who underwent proprioceptive neuromuscular facilitation (PNF). The hold-relax proprioceptive neuromuscular facilitation (PNF) technique was implemented to induce relaxation in the upper trapezius muscle, while the reverse PNF techniques were utilized to stabilize the scapula muscles. One session of scapular proprioceptive neuromuscular facilitation (PNF) has been shown to effectively enhance shoulder range of motion in flexion and abduction [ 28 , 32 ]. From this current study, the principle of the PNF pattern was a hold-relax technique in which the patient was told to hold a certain position for a specific amount of time in both the upper extremity and scapular pattern followed by a relaxation period. In their work, Rahman and colleagues [ 29 ] utilized rhythmic initiation and repetitive contraction of the scapula's anterior elevation and posterior depression as the strategies. Additionally, they clarified that the activation of the Golgi tendon organ, which triggers reflexive muscle relaxation, is likewise accountable for the augmentation of Range of Motion (ROM). Another factor that enhances shoulder function is the targeted application of the proprioceptive neuromuscular facilitation technique, which aims to alleviate tension in the muscles around the shoulder and limited joints, resulting in rapid improvements in range of motion. A randomized controlled trial conducted by Balci and colleagues [ 5 ] found that the application of the rhythmic initiation approach in scapular proprioceptive neuromuscular facilitation (PNF) enhances motion, induces relaxation in patients, enhances coordination, and restores normal motion. The technique of repetitive contractions facilitates a rise in both the active range of motion and strength, while also guiding the patient's movement towards the desired motion. Therefore, the present study confirms the effectiveness of the PNF technique in enhancing both the quality of life and the recovery process for individuals with adhesive capsulitis. Nevertheless, scapular PNF workouts did not directly induce these enhancements. The study posits that PNF has the potential to be efficacious when implemented alongside a consistent rehabilitation regimen in the long run. In their study, Jung and Chung [ 30 ] examined the effects of the scapular pattern and hold-relax technique of proprioceptive neuromuscular facilitation (PNF) on range of motion (ROM) and discomfort in a group of 30 individuals with acromioclavicular (AC) issues. Over the course of four weeks, they attended to the individuals experiencing discomfort and found that proprioceptive neuromuscular facilitation (PNF) proved to be efficacious in improving range of motion (ROM) and reducing pain. Overall, this research study has demonstrated that Proprioceptive Neuromuscular Facilitation (PNF) is superior to conventional or standard physiotherapy protocols in terms of pain reduction, improvement in range of motion (ROM), enhanced function, and reduced impairment. Assessing scapular stiffness following long-term treatment may yield different outcomes, as studies have demonstrated that the use of an exercise program during long-term treatment has resulted in improved shoulder discomfort and function. Since there were no participants who dropped out of the trial, there was no need to conduct an intention-to-treat analysis. A significant constraint of this investigation was that the trial therapists were unable to be blinded to the treatment allocation. The researcher attempted to mitigate the impact of unbinding by providing training to the trial therapists, blinding the assessor and patient about the trial allocation. Treatment was provided in two units to prevent trial contamination. As samples were collected only from CRP- Savar, Dhaka, Bangladesh, they could not represent the wider adhesive capsulitis population and the study lacks in generalize ability of results to the wider population. There may be another possible limitation that the training dosage or number of repetitions was not sufficient and more frequent training sessions may be required. The statistical analysis was confined to non-parametric analysis and no confidence interval of mean improvement was calculated. The study did not offer any follow-up for participants which was an essential component to find out the effectiveness of treatment for a longer period. Yet, this is the single trial of PNF in Bangladesh with a standard protocol, trial registration following guidelines of the EQUATOR network. So the study will add a new dimension to the clinical practice of AC in Bangladesh. Conclusions Proprioceptive neuromuscular facilitation technique (PNF) has greater clinical effect than conventional standard physiotherapy as exercise, stretching and electrotherapy in 6 weeks on pain remission, ROM improvement and remission of disability in shoulder adhesive capsulitis (AC). However, the results are promising and require further multicentre and multistage trials with external generalization. Abbreviations ADL Activities of daily living CRP Centre for the Rehabilitation of the Paralysed NCS Nerve conduction study NPRS Numeric pain rating scale PASS Power Analysis & Sample Size SPADI Shoulder pain and disability index Declarations Ethics approval and consent to participate Authors received the ethical permission to conduct the trial from Institutional Review Board (IRB), Bangladesh Health Professions Institute (BHPI), Centre for the Rehabilitation of the Paralysed (CRP), Savar, Dhaka-1343, Bangladesh (CRP-BHPI/IRB/06/2020/396). This trial is also listed in the Australian New Zealand Clinical Trial Registry (ACTRN) (ACTRN12621001299897) which is a primary clinical trial registry of the World Health Organization (WHO). The Helsinki Declaration was followed during the study, and we obtained informed consent from all subjects and their legal guardians. Consent for publication The participants were provided with informed consent before publishing any information. Availability of data and materials The data set is available at Khan, Abid Hasan (2024), “PNF_Adhesive_Capsulitis_Abid_Hasan_Khan”, Mendeley Data, doi: 10.17632/pwyh86kxrh.1 Competing interests The authors declare no competing interests. Funding This study was solely a self-funded work. Authors' contributions AHK wrote the main manuscript; KMAH made the necessary corrections; MFK, MZH, SJ & MSHB prepared the supplementary files and ER supervised the project. All authors reviewed and approved the manuscript. Acknowledgements We are very thankful to all the participants for their voluntary participation. We are also thankful to Tanvir Rahman, Md. Saifuddin Ahmed Parvez and Md. Kudrat Hayat for helping us with the SPADI language validation process. References Patel R, Urits I, Wolf J, Murthy A, Cornett EM, Jones MR, Ngo AL, Manchikanti L, Kaye AD, Viswanath O. A comprehensive update of adhesive capsulitis and minimally invasive treatment options. Psychopharmacol Bull. 2020;50(4):91. Neviaser AS, Neviaser RJ. Adhesive capsulitis of the shoulder. Amer Acad Ortho Surg. 2011;19(9):536–42. Deane KH, Jones D, Ellis-Hill C, Clarke CE, Playford ED, Ben-Shlomo Y. Physiotherapy for Parkinson’s disease: a comparison of techniques. Cochrane Database Syst Rev. 2001;1(1). Zuckerman JD, Rokito A. Frozen Shoulder: A consensus definition. J Shoulder Elb Surg. 2011;20(2):322–5. Balcı NC, Yuruk ZO, Zeybek A, Gulsen M, Tekindal MA. Acute effect of scapular proprioceptive neuromuscular facilitation (PNF) techniques and classic exercises in adhesive capsulitis: A randomized controlled trial. J Phys Ther Sci. 2016;28(4):1219–27. Noten S, Meeus M, Stassijns G, Van Glabbeek F, Verborgt O, Struyf F. Efficacy of different types of mobilization techniques in patients with primary adhesive capsulitis of the shoulder: A systematic review. Arch Phys Med Rehabil. 2016;97(5):815–25. Neviaser AS, Hannafin JA. Adhesive capsulitis. Am J Sports Med. 2010;38(11):2346–56. Al Dajah SB. Soft tissue mobilization and PNF improve range of motion and minimize pain level in shoulder impingement. J Phys Ther Sci. 2014;26(11):1803–5. Alito A, Cifalinò ME, Fontana JM, Verme F, Piterà P, Capodaglio P. Tackling Kinesiophobia in Chronic Shoulder Pain: A Case Report on the Combined Effect of Pain Education and Whole-Body Cryostimulation. J Clin Med. 2024;13(7):2094. Ramirez J. Adhesive capsulitis: diagnosis and management. Am Fam Physician. 2019;99(5):297–300. LeVasseur MR, Mancini R, Berthold M, Cusano DP, McCann A, Cote GP, Gomlinski MP, Mazzocca G. Acromioclavicular joint injuries: effective rehabilitation. Open Access J Sports Med. 2021;12:73–85. Yuan H, Wang K, Zhang QB, Wang F, Zhou Y. The effect of extracorporeal shock wave on joint capsule fibrosis based on A2AR-Nrf2/HO-1 pathway in a rat extending knee immobilization model. J Orthop Surg Res. 2023;18:930. Krishnan R, Björsell N, Gutierrez-Farewik EM, Smith C. A survey of human shoulder functional kinematic representations. Med Biol Eng Comput. 2019;57(2):339–67. Guiu-Tula FX, Cabanas-Valdés R, Sitjà-Rabert M, Urrútia G, Gómara-Toldrà N. The Efficacy of the proprioceptive neuromuscular facilitation (PNF) approach in stroke rehabilitation to improve basic activities of daily living and quality of life: a systematic review and meta-analysis protocol. BMJ open. 2017;7(12):e016739. Tedla JS, Sangadala DR. Proprioceptive neuromuscular facilitation techniques in adhesive capsulitis: a systematic review and meta-analysis. J Musculoskelet Neuronal Interact. 2019;19(4):482–91. Gao B, Li L, Shen P, Zhou Z, Xu P, Sun W, Zhang C, Song Q. Effects of proprioceptive neuromuscular facilitation stretching in relieving pain and balancing knee loading during stepping over obstacles among older adults with knee osteoarthritis: A randomized controlled trial. PLoS ONE. 2023;18(2):e0280941. Pourahmadi M, Sahebalam M, Bagheri R. Effectiveness of proprioceptive neuromuscular facilitation on pain intensity and functional disability in patients with low back pain: a systematic review and meta-analysis. Arch Bone Jt Surg. 2020;8(4):479. Lee JH, Kim EJ. The Effect of Diagonal Exercise Training for Neurorehabilitation on Functional Activity in Stroke Patients: A Pilot Study. Brain Sci. 2023;13(5):799. Hwang M, Lee S, Lim C. Effects of the proprioceptive neuromuscular facilitation technique on scapula function in office workers with scapula Dyskinesis. Medicina. 2021;57(4):332. Lin P, Yang M, Huang D, Lin H, Wang J, Zhong C, Guan L. Effect of proprioceptive neuromuscular facilitation technique on the treatment of frozen shoulder: A pilot randomized controlled trial. BMC Musculoskelet Disord. 2022;23:367. Akbaş E, Güneri S, Taş S, Erdem EU, Yüksel İ. The effects of additional proprioceptive neuromuscular facilitation over conventional therapy in patients with adhesive capsulitis. Turk J Physio Rehab. 2015;26(2):78–85. Hawker GA, Mian S, Kendzerska T, French M. Measures of adult pain: Visual Analog Scale for pain (Vas Pain), numeric rating scale for pain (NRS Pain), McGill Pain Questionnaire (MPQ), short-form McGill pain questionnaire (SF‐MPQ), chronic pain grade scale (CPGS), short form‐36 bodily pain scale (SF‐36 BPS), and measure of intermittent and constant osteoarthritis pain (ICOAP). Arthritis Care Res. 2011;63(S11):S240–52. Mullaney MJ, McHugh MP, Johnson CP, Tyler TF. Reliability of shoulder range of motion comparing a goniometer to a digital level. Physio Theor Prac. 2010;26(5):327–33. Choi S, Lee B-H, Chung E. The effects of stability exercises on shoulder pain and function of middle-aged women. J Phys Ther Sci. 2013;25(2):155–8. Sullivan L. Nonparametric Tests [Internet]. [cited 2024 May 21]. https://sphweb.bumc.bu.edu/otlt/mph-modules/bs/bs704_nonparametric/bs704_nonparametric_print.html Hindle K, Whitcomb T, Briggs W, Hong J. Proprioceptive Neuromuscular Facilitation (PNF): Its mechanisms and effects on range of motion and muscular function. J Human Kinetics. 2012;31(2012):105–13. Lee J-H, Park S-J, Na S-S. The effect of proprioceptive neuromuscular facilitation therapy on pain and function. J Phys Ther Sci. 2013;25(6):713–6. Al Dajah SB. Soft tissue mobilization and PNF improve range of motion and minimize pain level in shoulder impingement. J Phys Ther Sci. 2014;26(11):1803–5. Rahman RA, Sattar H, Zulfiqar A, Butt BS, Shakir S, Fatima N, et al. Effects of proprioceptive neuromuscular facilitation technique on Scapular dyskinesis in patients with subacute stroke. J Musculoskelet Surg Res. 2024;8:125–32. Jung J, Chung Y. Effects of combining both mobilization and hold-relax technique on the function of post-surgical patients with shoulder adhesive capsulitis. Phys Ther Rehab Sci. 2020;9(2):90–7. Das SK, Khan AH, Islam MS, Alam N, Nesaruddin M, Jonaied M, Alam TR, Shawon AA, Himel PR, Parvez MA. Specialized Physiotherapy and Pharmacological Management Strategies in Optimizing Chronic Lower Back Pain Rehabilitation. J Angio. 2024;8(5):9674. Ali E, Hossain F, Khan AH, Rahman MA, Rahman E, Islam S. Efficacy of Pilate’s Exercise with Postural Advice in the Management of Low Back Pain for Lower Limb Amputee Patients: A Randomised Control Trial. J Health Sci Med Res. 2023;41(6):2023966. Tables Table 1 Demographic and clinical characteristics of participants at baseline Variables Experimental group (n=40) Control group (n=40) p Demographic characteristics Age in years [median (IQR)] a 48 (43.25 to 60) 51 (45 to 57) 0.91 * Gender [ % (n) ] b Male 52.5 (21) 62.5 (25) 0.33 Female 47.5 (19) 37.5 (15) Education [ % (n) ] b Primary education 22.5 (9) 37.5 (15) 0.41 Completed SSC 32.5 (13) 22.5 (9) Completed HSC 22.5 (9) 22.5 (9) Bachelor and above degree 22.5 (9) 17.5 (7) Living area [ % (n) ] b Urban 27.5 (11) 42.5 (17) 0.09 Rural 47.5 (19) 37.5 (15) Semi-urban 25.0 (10) 20.0 (8) Occupation [ % (n) ] b Day labourer 15.0 (6) 22.5 (9) 0.72 Service holder 27.5 (11) 25.0 (10) Businessman 22.5 (9) 17.5 (7) Housewife 35.0 (14) 35.0 (14) Clinical characteristics History of trauma [ % (n) ] b Over use trauma 30.0 (12) 32.5 (13) 0.51 Direct trauma 60.0 (24) 62.5 (25) Psychological trauma 10.0 (4) 5.0 (2) Chronic illness [ % (n) ] b Diabetic Mellitus 17.5 (7) 20.0 (8) 0.46 Hypertension 47.5 (19) 45.0 (18) Heart disease 20.0 (8) 12.5 (5) Obesity 15.0 (6) 22.5 (9) Note: IQR, Inter quartile range; SSC, Secondary School Certificate; HSC, Higher Secondary Certificate; a Mann-Whitney U test and b Pearson Chi-square test; * baseline compatible Table 2 Baseline and after-treatment status of pain severity by Numeric Pain Rating Scale (NPRS) Outcome group Baseline After treatment Within group change scores Between group change scores Pain at resting position Experimental Median (IQR) Median (IQR)IQR Z p Z p 5 (3 to 6) 1 (0 to 2) 5.544 0.001 * 6.052 0.0001 *** Control 6 (3 to 6) 2 (2 to 3) 5.047 0.001 * Pain during rising arm sideways Experimental 9 (8 to 9) 2 (1 to 2) 5.489 0.001 * 7.518 0.0001 *** Control 8 (8 to 9) 5 (5 to 6) 5.358 0.001 * Pain during combing hair Experimental 9 (8 to 9) 2 (1 to 3) 5.542 0.001 * 4.990 0.0001 *** Control 9 (7 to 9) 5 (2 to 5) 5.256 0.001 * Note: *** Significant at 95% Confidence Level; Within-group analysis: Wilcoxon rank test; between-group analysis: Mann-Whitney U test Table 3 Baseline and after-treatment scores for pain, disability and total SPADI score by using Shoulder Pain and Disability Index (SPADI) Outcome group Baseline After treatment Within group change scores Between group change scores Pain intensity Experimental Median (IQR) Median (IQR) Z p Z p 72 (64.50 to 76) 26 (22 to 30) 5.519 0.001 * 7.648 0.0001 *** Control 75 (68.50 to 79.50) 50 (46 to 54) 2.820 0.032 * Disability level Experimental 64.50 (60 to 69.75) 22 (18 to 25) 5.516 0.001 * 7.696 0.0001 *** Control 67 (62 to 74) 44.50 (41 to 48) 2.685 0.028 * Total Score Experimental 67 (62 to 71) 23 (20 to 27) 5.517 0.001 * 7.707 0.0001 *** Control 70 (64.75 to 75) 46.50 (43 to 49) 2.515 0.021 * Note: *** Significant at 95% Confidence Level; Within-group analysis: Wilcoxon rank test; between-group analysis: Mann-Whitney U test Additional Declarations No competing interests reported. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4843786","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":357339268,"identity":"c3817338-3908-4e6c-a334-1ed2c0a2bba3","order_by":0,"name":"Abid Hasan Khan","email":"data:image/png;base64,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","orcid":"","institution":"Department of Physiotherapy and Rehabilitation, Jashore University of Science and Technology (JUST), Jashore-7408","correspondingAuthor":true,"prefix":"","firstName":"Abid","middleName":"Hasan","lastName":"Khan","suffix":""},{"id":357339269,"identity":"6dd7e500-5491-4cbc-a47a-8fc82347bd9f","order_by":1,"name":"Md. Saruar Hossain Bhuiyan","email":"","orcid":"","institution":"Department of Physiotherapy and Rehabilitation, Jashore University of Science and Technology (JUST), Jashore-7408","correspondingAuthor":false,"prefix":"","firstName":"Md.","middleName":"Saruar Hossain","lastName":"Bhuiyan","suffix":""},{"id":357339270,"identity":"95efb59c-4c80-423a-8b66-4f61ffa240b3","order_by":2,"name":"Md. Feroz Kabir","email":"","orcid":"","institution":"Department of Physiotherapy and Rehabilitation, Jashore University of Science and Technology (JUST), Jashore-7408","correspondingAuthor":false,"prefix":"","firstName":"Md.","middleName":"Feroz","lastName":"Kabir","suffix":""},{"id":357339271,"identity":"44d583d4-da37-42e5-b69f-9c618d732774","order_by":3,"name":"Md. Zahid Hossain","email":"","orcid":"","institution":"Department of Physiotherapy and Rehabilitation, Jashore University of Science and Technology (JUST), Jashore-7408","correspondingAuthor":false,"prefix":"","firstName":"Md.","middleName":"Zahid","lastName":"Hossain","suffix":""},{"id":357339272,"identity":"b2b4df78-2738-4d51-9f62-cd69d1640799","order_by":4,"name":"Sharmila Jahan","email":"","orcid":"","institution":"Department of Physiotherapy and Rehabilitation, Jashore University of Science and Technology (JUST), Jashore-7408","correspondingAuthor":false,"prefix":"","firstName":"Sharmila","middleName":"","lastName":"Jahan","suffix":""},{"id":357339273,"identity":"0abc614c-ab44-415e-9bae-5d057bcf625d","order_by":5,"name":"Kazi Md. Amran Hossain","email":"","orcid":"","institution":"Department of Physiotherapy and Rehabilitation, Jashore University of Science and Technology (JUST), Jashore-7408","correspondingAuthor":false,"prefix":"","firstName":"Kazi","middleName":"Md. Amran","lastName":"Hos","suffix":"Md."},{"id":357339274,"identity":"3e91cc74-b66b-4715-827c-6e22a5aa705a","order_by":6,"name":"Ehsanur Rahman","email":"","orcid":"","institution":"Department of Physiotherapy and Rehabilitation, Jashore University of Science and Technology (JUST), Jashore-7408","correspondingAuthor":false,"prefix":"","firstName":"Ehsanur","middleName":"","lastName":"Rahman","suffix":""}],"badges":[],"createdAt":"2024-08-01 17:19:58","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4843786/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4843786/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":65308733,"identity":"eff60c2a-c738-40ac-bfbe-823f3708591a","added_by":"auto","created_at":"2024-09-26 01:47:11","extension":"jpg","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":82578,"visible":true,"origin":"","legend":"\u003cp\u003eCONSORT flow diagram\u003c/p\u003e","description":"","filename":"Figures1.jpg","url":"https://assets-eu.researchsquare.com/files/rs-4843786/v1/c6c63f92aecf496b165cecda.jpg"},{"id":65308736,"identity":"75abaa41-c199-407e-8b2e-bd3608f2e4e2","added_by":"auto","created_at":"2024-09-26 01:47:11","extension":"jpg","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":81557,"visible":true,"origin":"","legend":"\u003cp\u003ePNF Application Procedure for Upper Extremity (A1-A3) and Scapula (B1, B2)\u003c/p\u003e","description":"","filename":"Figure2.jpg","url":"https://assets-eu.researchsquare.com/files/rs-4843786/v1/c69a2bd4a3d4585906edbdd7.jpg"},{"id":65308734,"identity":"b4c30b4e-0658-41f4-bf4b-c5d32be58dd1","added_by":"auto","created_at":"2024-09-26 01:47:11","extension":"jpg","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":26750,"visible":true,"origin":"","legend":"\u003cp\u003eEvaluation of median difference in theROM of shoulder movements after treatment\u003c/p\u003e","description":"","filename":"Figure3.jpg","url":"https://assets-eu.researchsquare.com/files/rs-4843786/v1/f749a2ecd0848f4c28c8b457.jpg"},{"id":65309906,"identity":"bbaa8b01-7460-4811-a7e2-569797e8fa8c","added_by":"auto","created_at":"2024-09-26 01:55:11","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":903706,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4843786/v1/9edab490-2948-4a41-b013-b4f4c1e90d9d.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Effectiveness of Proprioceptive Neuromuscular Facilitation Pattern on Upper Extremity and Scapula in Patients with Adhesive Capsulitis: A single centre assessor-blinded Randomised controlled trial (RCT)","fulltext":[{"header":"Background","content":"\u003cp\u003eAdhesive capsulitis (AC) is a musculoskeletal disorder characterized by painful limitation of upper limb activities that is associated with a combination of pain and limitation of passive movements [\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e]. The joint capsule of the shoulder becomes inflamed and gradual limitation of shoulder range of motion occurs; the condition is often explained as a stiff shoulder [\u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e]. Aetiology is unknown but cardiovascular disease, cervical spine conditions, diabetes, sudden trauma, stroke, Parkinson's disease, Coronary artery bypass grafting (CABG) and humeral fractures are often associated with AC [\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e]. Moreover, some intrinsic factors such as injuries to the rotator cuff muscles, long head of the biceps tendon, joint inflammation and calcification of the biceps tendon and other tendons may contribute to AC [\u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eAC is believed to be a self-limiting condition lasting two to three years, some studies have reported that up to 40% of patients have persistent symptoms and stiffness even after three years because pain and inflammation are self-remitting but the muscle atrophy and joint pathology are consistent if not treated [\u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e]. It is difficult to find an actual population prevalence of AC, the incidence is evident between 3% and 5% of the normal population in a given year [\u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e, \u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e]. AC greatly affects between forty to sixty years of aged people, with females being higher than males [\u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e]. About 20 to 30 percent of people having AC in one shoulder develop symptoms in the opposite shoulder after a certain period [\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eThe biomechanical abnormalities of the scapulohumeral rhythm and muscle control of the prime movers of the shoulder especially the deltoid and rotator cuff play vital role in developing AC associated musculoskeletal issues [\u003cspan citationid=\"CR8\" class=\"CitationRef\"\u003e8\u003c/span\u003e]. Moreover, the painful movement contributes to kinesiophobia that gradually hinders the flexibility of the muscles of shoulder and causes disruption to the scapula-humeral synergistic relationship [\u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e9\u003c/span\u003e]. The medical treatment for AC involves the use of nonsteroidal anti-inflammatory medications, short-term oral corticosteroids, intra-articular corticosteroid injections, physiotherapy, acupuncture, and hydro-dilatation [\u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e]. The role of physiotherapy is to facilitate the arthrokinematic motion of glenohumeral joint, acromio-clavicular joint and improve the osteokinemetics of these joints including scapula-thoracic articulation [\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e]. The gradual mobility and motion facilitate a range of motion (ROM), mimic the inflammatory mediators, and break down the scars of the joint capsule [\u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e12\u003c/span\u003e]. The convex-concave rule explains the necessity of neuro-muscular flexibility in improving the movement limitations in capsular patterns [\u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e13\u003c/span\u003e, \u003cspan citationid=\"CR32\" class=\"CitationRef\"\u003e32\u003c/span\u003e]. There are a couple of concepts in the management of AC; Proprioceptive neuromuscular facilitation (PNF) is one of them [\u003cspan citationid=\"CR14\" class=\"CitationRef\"\u003e14\u003c/span\u003e].\u003c/p\u003e \u003cp\u003ePNF has three theoretical mechanisms, autogenous inhibition, behaviour modification, and stress relaxation [\u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e]. The gate control theory of PNF is explained to enhance ROM that facilitates muscle activation [\u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e]. Evidence suggests PNF treatment increases blood flow by up to 71% in the administered muscles and eases subjective pain by up to 16% from baseline [\u003cspan citationid=\"CR14\" class=\"CitationRef\"\u003e14\u003c/span\u003e]. PNF added with conventional stretching and mobilization can decrease subjective pain by up to 21% [\u003cspan citationid=\"CR17\" class=\"CitationRef\"\u003e17\u003c/span\u003e]. In AC, PNF effects on stretch reflex mechanism and facilitates muscles by utilizing diagonal movement patterns to activate the bi-articular muscles of the shoulder [\u003cspan citationid=\"CR18\" class=\"CitationRef\"\u003e18\u003c/span\u003e].\u003c/p\u003e \u003cp\u003ePNF is a promising treatment among therapeutic approaches to improve pain, muscle flexibility, and increase ROM [\u003cspan citationid=\"CR17\" class=\"CitationRef\"\u003e17\u003c/span\u003e]. An all-encompassing strategy for AC management necessitates methods to enhance the effective functioning of the upper limbs, taking into account both the movement and stability of the scapula. The scapula pattern of Proprioceptive Neuromuscular Facilitation (PNF) simultaneously engages both upper extremity movement patterns and scapular motions [\u003cspan citationid=\"CR19\" class=\"CitationRef\"\u003e19\u003c/span\u003e]. The effects of PNF on the shoulder have been investigated in some studies [\u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e, \u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e20\u003c/span\u003e]. Nevertheless, there is a lack of research that explores the impact of upper extremity and scapular proprioceptive neuromuscular facilitation (PNF) exercises in the rehabilitation of the acromioclavicular (AC) joint. Hence, the objective of this study was to assess the efficacy of the Proprioceptive neuromuscular facilitation pattern (PNF) in treating adhesive capsulitis by comparing its efficiency on the upper extremity and scapula with traditional physiotherapy. Our hypothesis was PNF alone might have a superior effect compared to conventional exercise therapy in improving pain, muscle flexibility, ROM, shoulder function and disability in Adhesive capsulitis (AC).\u003c/p\u003e"},{"header":"Methods","content":"\u003cdiv id=\"Sec3\" class=\"Section2\"\u003e \u003ch2\u003eStudy Design\u003c/h2\u003e \u003cp\u003eAn accessor-blinded Randomised controlled trial (RCT) was conducted in the outpatient Physiotherapy musculoskeletal unit at the Department of Physiotherapy at the Centre for the Rehabilitation of the Paralysed (CRP), Savar, Dhaka- 1343, Bangladesh between May and December 2023. The population of interest was people having adhesive capsulitis diagnosis in hospital records. Ethical approval was received from the Institutional Review Board of Bangladesh Health Professions Institute (BHPI), and trial registration was obtained prospectively from the Australian New Zealand Clinical Trial registry platform (ACTRN12621001299897) (Date: 27/09/2021) [Annexure 1].\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec4\" class=\"Section2\"\u003e \u003ch2\u003eRecruitment of Patients and Randomization\u003c/h2\u003e \u003cp\u003eThe names and contact details of patients who had adhesive capsulitis were retrieved from hospital records of outpatient department software named Patient Data Management System (PDMS) at CRP. They were population frames. Then, patients were then contacted via a telephone call to invite them to the study. Patients interested in the study were screened against the inclusion/exclusion criteria using a telephone-administered questionnaire. The inclusion criteria were (1) adults aged between 21 and 70 years [\u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e21\u003c/span\u003e], (2) re-diagnosis as unilateral adhesive capsulitis (AC) by a qualified physiotherapist according to the Cyriax concept capsular pattern theory and (3) patient with shoulder pain at least one month [\u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e]. Patients were excluded if they reported (1) a history of surgery on the shoulder or manipulation under anaesthesia, (2) neurologic deficits affecting shoulder functioning during ADL or diagnosed as brachial plexopathy by nerve conduction study (NCS) (3) pain or disorders of the cervical spine, elbow, wrist, or hand and (4) other conditions involving the shoulder as rotator cuff tear, or tendinitis etc. [\u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e]. Eligible participants were referred to the musculoskeletal unit one and two of the Department of Physiotherapy, CRP for further screening with a concealed random allocation after the screening process. Before going for the treatment in every unit, there was a separate cubicle for the blinded assessor to complete the informed consent and baseline assessment. After the end of the treatment protocol, each patient re-entered the same room for outcome evaluation. A computerised random allocation technique using Microsoft Excel 2013 was used to assign the participants in both groups. The Consolidated Standards of Reporting Trial (CONSORT) was supplied in Fig.\u0026nbsp;1. The sample size was estimated using statistical power analysis processes with the assistance of the PASS 2005 program (NCSS, Kaysville, UT, USA). Based on a power analysis, it was determined that each group would require 30 individuals in order to achieve a power of 90% and maintain a type I error rate of 5%. To account for a dropout rate of 20% in the trial, we enrolled 40 participants in each group to guarantee a power of 90%. The power analysis conducted for our study revealed a statistical power of 90%, with adhesive capsulitis being the key outcome measure.\u003c/p\u003e \u003cp\u003e \u003cb\u003eFigure\u0026nbsp;1\u003c/b\u003e CONSORT flow diagram\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec5\" class=\"Section2\"\u003e \u003ch2\u003eOutcome Measurement\u003c/h2\u003e \u003cp\u003e \u003cstrong\u003eNumerical pain rating scale\u003c/strong\u003e \u003cp\u003eAs the primary outcome, we used the numeric pain rating scale (NPRS). The NPRS is a linear scale where the individual being evaluated indicates the intensity of their pain by marking a point on the line. The endpoints of the linear scale correspond to the farthest thresholds of pain, where 0 indicates the absence of pain, 1\u0026ndash;3 denotes mild discomfort, 4\u0026ndash;6 signifies moderate pain, and 7\u0026ndash;10 represents the most excruciating agony ever encountered [\u003cspan citationid=\"CR22\" class=\"CitationRef\"\u003e22\u003c/span\u003e].\u003c/p\u003e \u003c/p\u003e \u003cp\u003e \u003cstrong\u003eGoniometer\u003c/strong\u003e \u003cp\u003eTo measure another primary outcome goniometer was used to measure ROM. We assessed the range of motion at the initial assessment and post-test assessment with the same goniometer to ensure consistency. A goniometer is a valid and reliable tool for measuring ROM globally [\u003cspan citationid=\"CR23\" class=\"CitationRef\"\u003e23\u003c/span\u003e].\u003c/p\u003e \u003c/p\u003e \u003cp\u003e \u003cstrong\u003eShoulder pain and disability index (SPADI)\u003c/strong\u003e \u003cp\u003eAdditionally, we assessed function, pain level, and disability using the SPADI scale as a supplementary measure. The system is composed of two sections, one dedicated to pain-inducing activities and the other focused on functional tasks, the pain dimension comprises 5 inquiries regarding the intensity of an individual's pain. The assessment of functional activities involved the use of eight specific questions aimed at determining the level of difficulty that individuals experience in performing various daily tasks that require the use of their upper limbs. The SPADI can be completed by a patient in a time frame of five to ten minutes and is the only dependable and accurate measure specifically designed for assessing the shoulder [\u003cspan citationid=\"CR24\" class=\"CitationRef\"\u003e24\u003c/span\u003e].\u003c/p\u003e \u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec6\" class=\"Section2\"\u003e \u003ch2\u003eIntervention\u003c/h2\u003e \u003cdiv id=\"Sec7\" class=\"Section3\"\u003e \u003ch2\u003eExperimental group\u003c/h2\u003e \u003cp\u003eWithin this group, we employed the Proprioceptive Neuromuscular Facilitation Technique (PNF) specifically for the upper extremity [\u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e21\u003c/span\u003e]. The patient was resting on their back with their arms positioned alongside their torso. The individuals were instructed to loosen the tension in their shoulder muscles. The therapist positioned themselves next to the impacted side of the patient. The technique used was the \"Flexion-abduction-external rotation\" (D2F pattern) with a straight elbow pattern of proprioceptive neuromuscular facilitation (PNF) using the \"hold relax\" technique. This equipment is designed to provide relaxation by using isometric contractions against maximum resistance. It is intended to enhance the passive range of motion and reduce pain. The individual executed isometric contractions for 5\u0026ndash;8 seconds, applying maximal resistance. These contractions were specifically targeted to prevent the opposing muscles from contracting and included a rotation at the point of maximum joint movement. The technique was done multiple times until reaching the point of movement restrictions and then advanced to additional stages. When implementing the D2F pattern with a straight elbow pattern of PNF, the therapists used their hands to grasp the patient's upper limb on the side opposite to the hip. The patient's shoulder was positioned in extension, adduction, and internal rotation, while the elbow was extended and the forearm was pronated. The physiotherapist thereafter instructs the patient to elevate his hand above his head. The patient endeavoured to execute this motion by engaging in shoulder flexion, abduction, and external rotation. During these movements, the therapist would stabilize the patient's arm with his other hand (Fig.\u0026nbsp;2). The duration of treatment was 15 minutes for every 10 sets of PNF. There were 5 repetitions per set, with 5-second hold time, and 2 seconds of rest time. There was no progression of sets. Treatment was provided for 6 weeks and 4 sessions per week in alternative days.\u003c/p\u003e \u003cp\u003eThe second technique was the Proprioceptive Neuromuscular Facilitation Technique for the Scapula and it has two patterns (Fig.\u0026nbsp;2). Firstly anterior elevation \u0026amp; posterior depression. The patient reclines on the unaffected side, while the therapist positions themselves behind the patient, with one hand resting on the upper edge of the scapula and the other on the lower corner of the scapula. The therapist provided the patient with manual resistance and directed them to perform scapular movements by pushing up and down. The duration of treatment was 7 minutes for 5 sets. Treatment repetitions were 5 repetitions/set, with hold time for 5 second\u003cb\u003es\u003c/b\u003e, and rest time for 2 seconds. The treatment was given for a total of 6 weeks, 4 sessions per week.\u003c/p\u003e \u003cp\u003eAdditionally, in cases of posterior elevation and anterior depression, the patient is positioned on the side that is not affected. The therapist stood behind the patient, with one hand positioned on the superior border of the scapula and the other on the inferior angle of the scapula. The patient received instructions to exert force in a backward direction and apply pressure to the front of the scapula while the therapist provided resistance. The duration of treatment was 8 minutes per 5 sets. Repetitions were 5 repetitions/set, hold time for 5 seconds, and rest time for 2 seconds. The treatment was provided for a total of 6 weeks, 4 sessions per week.\u003c/p\u003e \u003cp\u003e \u003cb\u003eFigure\u0026nbsp;2\u003c/b\u003e PNF Application Procedure for Upper Extremity (A1-A3) and Scapula (B1, B2)\u003c/p\u003e \u003c/div\u003e \u003c/div\u003e \u003cdiv id=\"Sec8\" class=\"Section2\"\u003e \u003ch2\u003eControl group\u003c/h2\u003e \u003cp\u003eThe control group was given conventional physiotherapy only according to the patient\u0026rsquo;s response to treatment. Both groups underwent a standard intervention program consisting of conventional physiotherapy techniques, such as Capsular stretching, Accessory movements, Pendulum exercise, Pulley exercise, Infrared radiation applied to the affected shoulder for 15 minutes, and Ultrasound therapy tailored to the patient's condition and prescribed frequency. The control group received 30 minutes of treatment per session for 4 times a week for up to 6 weeks. The control treatment was according to the standard physiotherapy exercise protocol set by the outpatient musculoskeletal unit, Department of Physiotherapy, CRP, Savar, Dhaka-1343, Bangladesh.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec9\" class=\"Section2\"\u003e \u003ch2\u003eStatistical Analysis\u003c/h2\u003e \u003cp\u003eA parametric test was used to analyse interval or ratio data and a non-parametric test was used to analyse the nominal/ordinal data. The normality of data was checked by the Kolmogorov-Smirnov test. The value of the Kolmogorov-Smirnov test is less than 0.05, which indicates that the data distribution was not normal [\u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e25\u003c/span\u003e]. So, descriptive statistics like median and interquartile range (IQR) were used to analyse the sociodemographic information where the between-group mean difference analysis of pain intensity, improvement of ROM of different movements of shoulder and improvement of functional performance of the participants was analysed by Mann-Whitney U-test and the within-group analysis of pain intensity, improvement of ROM of different movements of shoulder and improvement of functional performance of the participants was done by Wilcoxon signed rank test. The alpha value was set as p\u0026thinsp;\u0026lt;\u0026thinsp;0.05.\u003c/p\u003e \u003c/div\u003e"},{"header":"Results","content":"\u003cdiv id=\"Sec11\" class=\"Section2\"\u003e \u003ch2\u003eDemographic and clinical characteristics\u003c/h2\u003e \u003cp\u003eThe sociodemographic and clinical information are summarised in Table\u0026nbsp;1. The median age of the participants in the experimental group was 48 (43.25 to 60) and in the control group 51 (45 to 57) years. In both groups, the male participants number was the maximum, 52.5% (n\u0026thinsp;=\u0026thinsp;21) in the experimental group and 62.5% (n\u0026thinsp;=\u0026thinsp;25) in the control group. Regarding educational status, most participants 32.5% (n\u0026thinsp;=\u0026thinsp;13) completed SSC in the experimental group whereas 37.5% (n\u0026thinsp;=\u0026thinsp;15) completed primary education in the control group. Most of the participants in the experimental group lived in the rural areas 47.5% (n\u0026thinsp;=\u0026thinsp;19) but in the control group, they were mostly from urban areas, 42.5% (n\u0026thinsp;=\u0026thinsp;17). Except for housewives, most of the participants were service holders in both groups, 27.5% (n\u0026thinsp;=\u0026thinsp;11) in the experimental group and 25.0% (n\u0026thinsp;=\u0026thinsp;10) in the control group. Regarding clinical characteristics, most of the participants had direct trauma in both groups, 60.0% (n\u0026thinsp;=\u0026thinsp;24) in the experimental group and 62.5% (n\u0026thinsp;=\u0026thinsp;25) in the control group. Hypertension was the most common chronic illness in both groups, 47.5% (n\u0026thinsp;=\u0026thinsp;19) in the experimental group and 45.0% (n\u0026thinsp;=\u0026thinsp;18) in the control group.\u003c/p\u003e \u003cp\u003e \u003cb\u003eTable\u0026nbsp;1\u003c/b\u003e Demographic and clinical characteristics of participants at baseline\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec12\" class=\"Section2\"\u003e \u003ch2\u003ePain severity\u003c/h2\u003e \u003cp\u003eBoth group participants were screened for their pain severity in the shoulder joint (Table\u0026nbsp;2). Based on some common activities patients were evaluated for pain in resting position where the within-group analysis showed significant change was found in both experimental group and control group (p\u0026thinsp;\u0026lt;\u0026thinsp;0.001). In the case of pain during rising arm sideways, within-group analysis showed significant change was found in both the experimental group and control group (p\u0026thinsp;\u0026lt;\u0026thinsp;0.001) and for pain during combing hair, within-group analysis showed significant change was found in both experimental group and control group (p\u0026thinsp;\u0026lt;\u0026thinsp;0.001). For between-group analyses, significant changes were found in all three activities of the shoulder joint (p\u0026thinsp;\u0026lt;\u0026thinsp;0.001).\u003c/p\u003e \u003cp\u003e \u003cb\u003eTable\u0026nbsp;2\u003c/b\u003e Baseline and after-treatment status of pain severity by Numeric Pain Rating Scale (NPRS)\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec13\" class=\"Section2\"\u003e \u003ch2\u003eRange of Motion (ROM)\u003c/h2\u003e \u003cp\u003eLimitation in shoulder joint was checked for only three movements shoulder abduction, lateral rotation and medial rotation. A significant median difference was observed after providing treatment between control and experimental group participants in terms of shoulder lateral and medial rotation movement (p\u0026thinsp;\u0026lt;\u0026thinsp;0.001) but not for shoulder abduction (p\u0026thinsp;\u0026gt;\u0026thinsp;0.5). (Fig.\u0026nbsp;3)\u003c/p\u003e \u003cp\u003e \u003cb\u003eFigure\u0026nbsp;3\u003c/b\u003e Evaluation of median difference in the ROM of shoulder movements after treatment\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec14\" class=\"Section2\"\u003e \u003ch2\u003eShoulder pain and disability index\u003c/h2\u003e \u003cp\u003eParticipants were screened by Shoulder pain and disability index at baseline and after completing the whole treatment sessions (Table\u0026nbsp;3). In the case of pain intensity, within-group analysis showed significant change was found in both the experimental group (p\u0026thinsp;\u0026lt;\u0026thinsp;0.001) and control group (p\u0026thinsp;\u0026lt;\u0026thinsp;0.05). In the case of disability level, within-group analysis showed significant change was found in both the experimental group (p\u0026thinsp;\u0026lt;\u0026thinsp;0.001) and control group (p\u0026thinsp;\u0026lt;\u0026thinsp;0.05) and for total SPADI score, within-group analysis showed significant change was found in both experimental group (p\u0026thinsp;\u0026lt;\u0026thinsp;0.001) and control group (p\u0026thinsp;=\u0026thinsp;0.021). For between-group analyses, significant changes were found in all parameters of SPADI (p\u0026thinsp;\u0026lt;\u0026thinsp;0.001).\u003c/p\u003e \u003cp\u003e \u003cb\u003eTable\u0026nbsp;3\u003c/b\u003e Baseline and after-treatment scores for pain, disability and total SPADI score by using Shoulder Pain and Disability Index (SPADI)\u003c/p\u003e \u003c/div\u003e"},{"header":"Discussion","content":"\u003cp\u003eThis study was carried out to determine the effect of PNF compared to standard physiotherapy in adhesive capsulitis in a Bangladeshi setting. The primary objective was to determine the superiority of PNF in relieving pain, improving ROM and improving the functional and disability status of AC. The objective was successfully examined with statistically significant superiority results in the PNF group in all indicators. In consideration, it was revealed that the mean age of the participants was 50 (44.25 to 60) years and among men 21.3% were government and non-government service holders and among women 42.5% were housewives. As for major working positions, 66.3% worked in a sitting position and 21.3% worked in a standing position. Among the participants, 55% had a history of overuse injury. These incidences are indicating the relationship between age and working characteristics with adhesive capsulitis. A randomized controlled study conducted on 36 participants by Akbas and colleagues [\u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e21\u003c/span\u003e] revealed that the mean age of the participants was 53.94\u0026thinsp;\u0026plusmn;\u0026thinsp;9.38 and only 11 of 36 patients (30.6%) with adhesive capsulitis were still working in various jobs and sectors actively, rest of them weren\u0026rsquo;t working (15 retired \u0026minus;\u0026thinsp;10 unemployed) as of 69.4%.\u003c/p\u003e \u003cp\u003eThis study demonstrated the efficacy of scapular proprioceptive neuromuscular facilitation (PNF) exercises in reducing pain intensity during rest, arm abduction, and hair combing. Additionally, the study revealed that the PNF group had greater improvement in shoulder range of motion compared to the control group. However, the distinction was not statistically significant for abduction movements. Nevertheless, it was shown to be statistically significant for external and internal rotation movements. Additionally, significant improvements were observed in functional performance, such as discomfort and handicap status. In a randomized controlled study conducted by Lin and colleagues [\u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e20\u003c/span\u003e], it was found that both the scapular proprioceptive neuromuscular facilitation (PNF) group and the conventional physiotherapy group showed significant improvement. However, the PNF group had a greater reduction in pain and disability compared to the conventional group, as indicated by the mean value.\u003c/p\u003e \u003cp\u003eIt was elicited that, upper extremity and scapula PNF patterns provide additional benefit in only abduction movement of the shoulder, although do not provide benefit in resting pain, pain in an activity like raising the arm sideways or combing hair, internal and external rotation movement parameters in management of adhesive capsulitis. The within-group analysis of the experimental group revealed a significant reduction in pain at a statistically significant level of p\u0026thinsp;\u0026lt;\u0026thinsp;0.001. The underlying idea here is that PNF (Proprioceptive Neuromuscular Facilitation) has been scientifically demonstrated to generate a pain-relieving effect by means of a gate control mechanism. The PNF approach applies pressure and proprioceptive inputs that reach the spinal level, effectively inhibiting the entry and transmission of pain signals [\u003cspan citationid=\"CR26\" class=\"CitationRef\"\u003e26\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eIn this study, there is a significant improvement in shoulder movement especially in internal rotation and external rotation found in the post-interventional increase of Range of motion in the PNF group. This can be occurred due to an increment in excitability and a decrease in response time. In their randomized study, Lee and colleagues [\u003cspan citationid=\"CR27\" class=\"CitationRef\"\u003e27\u003c/span\u003e] found that the combination of general physiotherapy techniques (including a 20-minute hot pack, 5 minutes of US therapy, and 20 minutes of TENS) was effective in improving pain and features associated with myofascial pain syndrome. They observed this positive outcome in approximately 32 participants who underwent proprioceptive neuromuscular facilitation (PNF). The hold-relax proprioceptive neuromuscular facilitation (PNF) technique was implemented to induce relaxation in the upper trapezius muscle, while the reverse PNF techniques were utilized to stabilize the scapula muscles. One session of scapular proprioceptive neuromuscular facilitation (PNF) has been shown to effectively enhance shoulder range of motion in flexion and abduction [\u003cspan citationid=\"CR28\" class=\"CitationRef\"\u003e28\u003c/span\u003e, \u003cspan citationid=\"CR32\" class=\"CitationRef\"\u003e32\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eFrom this current study, the principle of the PNF pattern was a hold-relax technique in which the patient was told to hold a certain position for a specific amount of time in both the upper extremity and scapular pattern followed by a relaxation period. In their work, Rahman and colleagues [\u003cspan citationid=\"CR29\" class=\"CitationRef\"\u003e29\u003c/span\u003e] utilized rhythmic initiation and repetitive contraction of the scapula's anterior elevation and posterior depression as the strategies. Additionally, they clarified that the activation of the Golgi tendon organ, which triggers reflexive muscle relaxation, is likewise accountable for the augmentation of Range of Motion (ROM).\u003c/p\u003e \u003cp\u003eAnother factor that enhances shoulder function is the targeted application of the proprioceptive neuromuscular facilitation technique, which aims to alleviate tension in the muscles around the shoulder and limited joints, resulting in rapid improvements in range of motion. A randomized controlled trial conducted by Balci and colleagues [\u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e] found that the application of the rhythmic initiation approach in scapular proprioceptive neuromuscular facilitation (PNF) enhances motion, induces relaxation in patients, enhances coordination, and restores normal motion. The technique of repetitive contractions facilitates a rise in both the active range of motion and strength, while also guiding the patient's movement towards the desired motion. Therefore, the present study confirms the effectiveness of the PNF technique in enhancing both the quality of life and the recovery process for individuals with adhesive capsulitis. Nevertheless, scapular PNF workouts did not directly induce these enhancements. The study posits that PNF has the potential to be efficacious when implemented alongside a consistent rehabilitation regimen in the long run. In their study, Jung and Chung [\u003cspan citationid=\"CR30\" class=\"CitationRef\"\u003e30\u003c/span\u003e] examined the effects of the scapular pattern and hold-relax technique of proprioceptive neuromuscular facilitation (PNF) on range of motion (ROM) and discomfort in a group of 30 individuals with acromioclavicular (AC) issues. Over the course of four weeks, they attended to the individuals experiencing discomfort and found that proprioceptive neuromuscular facilitation (PNF) proved to be efficacious in improving range of motion (ROM) and reducing pain.\u003c/p\u003e \u003cp\u003eOverall, this research study has demonstrated that Proprioceptive Neuromuscular Facilitation (PNF) is superior to conventional or standard physiotherapy protocols in terms of pain reduction, improvement in range of motion (ROM), enhanced function, and reduced impairment. Assessing scapular stiffness following long-term treatment may yield different outcomes, as studies have demonstrated that the use of an exercise program during long-term treatment has resulted in improved shoulder discomfort and function. Since there were no participants who dropped out of the trial, there was no need to conduct an intention-to-treat analysis.\u003c/p\u003e \u003cp\u003eA significant constraint of this investigation was that the trial therapists were unable to be blinded to the treatment allocation. The researcher attempted to mitigate the impact of unbinding by providing training to the trial therapists, blinding the assessor and patient about the trial allocation. Treatment was provided in two units to prevent trial contamination. As samples were collected only from CRP- Savar, Dhaka, Bangladesh, they could not represent the wider adhesive capsulitis population and the study lacks in generalize ability of results to the wider population. There may be another possible limitation that the training dosage or number of repetitions was not sufficient and more frequent training sessions may be required. The statistical analysis was confined to non-parametric analysis and no confidence interval of mean improvement was calculated. The study did not offer any follow-up for participants which was an essential component to find out the effectiveness of treatment for a longer period. Yet, this is the single trial of PNF in Bangladesh with a standard protocol, trial registration following guidelines of the EQUATOR network. So the study will add a new dimension to the clinical practice of AC in Bangladesh.\u003c/p\u003e"},{"header":"Conclusions","content":"\u003cp\u003eProprioceptive neuromuscular facilitation technique (PNF) has greater clinical effect than conventional standard physiotherapy as exercise, stretching and electrotherapy in 6 weeks on pain remission, ROM improvement and remission of disability in shoulder adhesive capsulitis (AC). However, the results are promising and require further multicentre and multistage trials with external generalization.\u003c/p\u003e"},{"header":"Abbreviations","content":"\u003cp\u003eADL Activities of daily living\u003c/p\u003e \u003cp\u003eCRP Centre for the Rehabilitation of the Paralysed\u003c/p\u003e \u003cp\u003eNCS Nerve conduction study\u003c/p\u003e \u003cp\u003eNPRS Numeric pain rating scale\u003c/p\u003e \u003cp\u003ePASS Power Analysis \u0026amp; Sample Size\u003c/p\u003e \u003cp\u003eSPADI Shoulder pain and disability index\u003c/p\u003e "},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eEthics approval and consent to participate\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAuthors\u0026nbsp;received\u0026nbsp;the ethical permission to conduct the trial from Institutional Review Board (IRB), Bangladesh Health Professions Institute (BHPI), Centre for the Rehabilitation of the Paralysed (CRP), Savar, Dhaka-1343, Bangladesh (CRP-BHPI/IRB/06/2020/396). This trial is also listed in the Australian New Zealand Clinical Trial Registry (ACTRN) (ACTRN12621001299897) which is a primary clinical trial registry of the World Health Organization (WHO). The Helsinki Declaration was followed during the study, and we obtained informed consent from all subjects and their legal guardians.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConsent for publication\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe participants were provided with informed consent before publishing any information.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAvailability of data and materials\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe data set is available at Khan, Abid Hasan (2024), \u0026ldquo;PNF_Adhesive_Capsulitis_Abid_Hasan_Khan\u0026rdquo;, Mendeley Data, doi: 10.17632/pwyh86kxrh.1\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompeting interests\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe authors declare no competing interests.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFunding\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThis study was solely a self-funded work.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAuthors\u0026apos; contributions\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAHK wrote the main manuscript; KMAH made the necessary corrections; MFK, MZH, SJ \u0026amp; MSHB prepared the supplementary files and ER supervised the project. All authors reviewed and approved the manuscript.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAcknowledgements\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eWe are very thankful to all the participants for their voluntary participation. We are also thankful to Tanvir Rahman, Md. Saifuddin Ahmed Parvez and Md. Kudrat Hayat for helping us with the SPADI language validation process.\u0026nbsp;\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\u003cli\u003e\u003cspan\u003ePatel R, Urits I, Wolf J, Murthy A, Cornett EM, Jones MR, Ngo AL, Manchikanti L, Kaye AD, Viswanath O. A comprehensive update of adhesive capsulitis and minimally invasive treatment options. Psychopharmacol Bull. 2020;50(4):91.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eNeviaser AS, Neviaser RJ. Adhesive capsulitis of the shoulder. Amer Acad Ortho Surg. 2011;19(9):536\u0026ndash;42.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eDeane KH, Jones D, Ellis-Hill C, Clarke CE, Playford ED, Ben-Shlomo Y. Physiotherapy for Parkinson\u0026rsquo;s disease: a comparison of techniques. Cochrane Database Syst Rev. 2001;1(1).\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eZuckerman JD, Rokito A. Frozen Shoulder: A consensus definition. J Shoulder Elb Surg. 2011;20(2):322\u0026ndash;5.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eBalcı NC, Yuruk ZO, Zeybek A, Gulsen M, Tekindal MA. Acute effect of scapular proprioceptive neuromuscular facilitation (PNF) techniques and classic exercises in adhesive capsulitis: A randomized controlled trial. J Phys Ther Sci. 2016;28(4):1219\u0026ndash;27.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eNoten S, Meeus M, Stassijns G, Van Glabbeek F, Verborgt O, Struyf F. Efficacy of different types of mobilization techniques in patients with primary adhesive capsulitis of the shoulder: A systematic review. Arch Phys Med Rehabil. 2016;97(5):815\u0026ndash;25.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eNeviaser AS, Hannafin JA. Adhesive capsulitis. Am J Sports Med. 2010;38(11):2346\u0026ndash;56.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eAl Dajah SB. Soft tissue mobilization and PNF improve range of motion and minimize pain level in shoulder impingement. J Phys Ther Sci. 2014;26(11):1803\u0026ndash;5.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eAlito A, Cifalin\u0026ograve; ME, Fontana JM, Verme F, Piter\u0026agrave; P, Capodaglio P. Tackling Kinesiophobia in Chronic Shoulder Pain: A Case Report on the Combined Effect of Pain Education and Whole-Body Cryostimulation. J Clin Med. 2024;13(7):2094.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eRamirez J. Adhesive capsulitis: diagnosis and management. Am Fam Physician. 2019;99(5):297\u0026ndash;300.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eLeVasseur MR, Mancini R, Berthold M, Cusano DP, McCann A, Cote GP, Gomlinski MP, Mazzocca G. Acromioclavicular joint injuries: effective rehabilitation. Open Access J Sports Med. 2021;12:73\u0026ndash;85.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eYuan H, Wang K, Zhang QB, Wang F, Zhou Y. The effect of extracorporeal shock wave on joint capsule fibrosis based on A2AR-Nrf2/HO-1 pathway in a rat extending knee immobilization model. J Orthop Surg Res. 2023;18:930.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eKrishnan R, Bj\u0026ouml;rsell N, Gutierrez-Farewik EM, Smith C. A survey of human shoulder functional kinematic representations. Med Biol Eng Comput. 2019;57(2):339\u0026ndash;67.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eGuiu-Tula FX, Cabanas-Vald\u0026eacute;s R, Sitj\u0026agrave;-Rabert M, Urr\u0026uacute;tia G, G\u0026oacute;mara-Toldr\u0026agrave; N. The Efficacy of the proprioceptive neuromuscular facilitation (PNF) approach in stroke rehabilitation to improve basic activities of daily living and quality of life: a systematic review and meta-analysis protocol. BMJ open. 2017;7(12):e016739.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eTedla JS, Sangadala DR. Proprioceptive neuromuscular facilitation techniques in adhesive capsulitis: a systematic review and meta-analysis. J Musculoskelet Neuronal Interact. 2019;19(4):482\u0026ndash;91.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eGao B, Li L, Shen P, Zhou Z, Xu P, Sun W, Zhang C, Song Q. Effects of proprioceptive neuromuscular facilitation stretching in relieving pain and balancing knee loading during stepping over obstacles among older adults with knee osteoarthritis: A randomized controlled trial. PLoS ONE. 2023;18(2):e0280941.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003ePourahmadi M, Sahebalam M, Bagheri R. Effectiveness of proprioceptive neuromuscular facilitation on pain intensity and functional disability in patients with low back pain: a systematic review and meta-analysis. Arch Bone Jt Surg. 2020;8(4):479.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eLee JH, Kim EJ. The Effect of Diagonal Exercise Training for Neurorehabilitation on Functional Activity in Stroke Patients: A Pilot Study. Brain Sci. 2023;13(5):799.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eHwang M, Lee S, Lim C. Effects of the proprioceptive neuromuscular facilitation technique on scapula function in office workers with scapula Dyskinesis. Medicina. 2021;57(4):332.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eLin P, Yang M, Huang D, Lin H, Wang J, Zhong C, Guan L. Effect of proprioceptive neuromuscular facilitation technique on the treatment of frozen shoulder: A pilot randomized controlled trial. BMC Musculoskelet Disord. 2022;23:367.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eAkbaş E, G\u0026uuml;neri S, Taş S, Erdem EU, Y\u0026uuml;ksel İ. The effects of additional proprioceptive neuromuscular facilitation over conventional therapy in patients with adhesive capsulitis. Turk J Physio Rehab. 2015;26(2):78\u0026ndash;85.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eHawker GA, Mian S, Kendzerska T, French M. Measures of adult pain: Visual Analog Scale for pain (Vas Pain), numeric rating scale for pain (NRS Pain), McGill Pain Questionnaire (MPQ), short-form McGill pain questionnaire (SF‐MPQ), chronic pain grade scale (CPGS), short form‐36 bodily pain scale (SF‐36 BPS), and measure of intermittent and constant osteoarthritis pain (ICOAP). Arthritis Care Res. 2011;63(S11):S240\u0026ndash;52.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eMullaney MJ, McHugh MP, Johnson CP, Tyler TF. Reliability of shoulder range of motion comparing a goniometer to a digital level. Physio Theor Prac. 2010;26(5):327\u0026ndash;33.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eChoi S, Lee B-H, Chung E. The effects of stability exercises on shoulder pain and function of middle-aged women. J Phys Ther Sci. 2013;25(2):155\u0026ndash;8.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eSullivan L. Nonparametric Tests [Internet]. [cited 2024 May 21]. \u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttps://sphweb.bumc.bu.edu/otlt/mph-modules/bs/bs704_nonparametric/bs704_nonparametric_print.html\u003c/span\u003e\u003cspan address=\"https://sphweb.bumc.bu.edu/otlt/mph-modules/bs/bs704_nonparametric/bs704_nonparametric_print.html\" targettype=\"URL\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eHindle K, Whitcomb T, Briggs W, Hong J. Proprioceptive Neuromuscular Facilitation (PNF): Its mechanisms and effects on range of motion and muscular function. J Human Kinetics. 2012;31(2012):105\u0026ndash;13.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eLee J-H, Park S-J, Na S-S. The effect of proprioceptive neuromuscular facilitation therapy on pain and function. J Phys Ther Sci. 2013;25(6):713\u0026ndash;6.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eAl Dajah SB. Soft tissue mobilization and PNF improve range of motion and minimize pain level in shoulder impingement. J Phys Ther Sci. 2014;26(11):1803\u0026ndash;5.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eRahman RA, Sattar H, Zulfiqar A, Butt BS, Shakir S, Fatima N, et al. Effects of proprioceptive neuromuscular facilitation technique on Scapular dyskinesis in patients with subacute stroke. J Musculoskelet Surg Res. 2024;8:125\u0026ndash;32.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eJung J, Chung Y. Effects of combining both mobilization and hold-relax technique on the function of post-surgical patients with shoulder adhesive capsulitis. Phys Ther Rehab Sci. 2020;9(2):90\u0026ndash;7.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eDas SK, Khan AH, Islam MS, Alam N, Nesaruddin M, Jonaied M, Alam TR, Shawon AA, Himel PR, Parvez MA. Specialized Physiotherapy and Pharmacological Management Strategies in Optimizing Chronic Lower Back Pain Rehabilitation. J Angio. 2024;8(5):9674.\u003c/span\u003e\u003c/li\u003e \u003cli\u003e\u003cspan\u003eAli E, Hossain F, Khan AH, Rahman MA, Rahman E, Islam S. Efficacy of Pilate\u0026rsquo;s Exercise with Postural Advice in the Management of Low Back Pain for Lower Limb Amputee Patients: A Randomised Control Trial. J Health Sci Med Res. 2023;41(6):2023966.\u003c/span\u003e\u003c/li\u003e\u003c/ol\u003e"},{"header":"Tables","content":"\u003cp\u003e\u003cstrong\u003eTable 1\u003c/strong\u003e Demographic and clinical characteristics of participants at baseline\u003c/p\u003e\n\u003ctable border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd width=\"36.10648918469218%\"\u003e\n \u003cp\u003e\u003cstrong\u003eVariables\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"26.788685524126457%\"\u003e\n \u003cp\u003e\u003cstrong\u003eExperimental group (n=40)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.124792013311147%\"\u003e\n \u003cp\u003e\u003cstrong\u003eControl group (n=40)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.980033277870216%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u003cem\u003ep\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"100%\" colspan=\"4\"\u003e\n \u003cp\u003e\u003cstrong\u003eDemographic characteristics\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"36.10648918469218%\" valign=\"top\"\u003e\n \u003cp\u003eAge in years [median (IQR)]\u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"26.788685524126457%\" valign=\"top\"\u003e\n \u003cp\u003e48 (43.25 to 60)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.124792013311147%\" valign=\"top\"\u003e\n \u003cp\u003e51 (45 to 57)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.980033277870216%\" valign=\"top\"\u003e\n \u003cp\u003e0.91\u003csup\u003e*\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"100%\" colspan=\"4\" valign=\"top\"\u003e\n \u003cp\u003eGender [\u003cem\u003e% (n)\u003c/em\u003e]\u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"36.10648918469218%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;Male\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"26.788685524126457%\"\u003e\n \u003cp\u003e52.5 (21)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.124792013311147%\"\u003e\n \u003cp\u003e62.5 (25)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.980033277870216%\" rowspan=\"2\"\u003e\n \u003cp\u003e0.33\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"41.02079395085066%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;Female\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.434782608695652%\"\u003e\n \u003cp\u003e47.5 (19)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"28.544423440453688%\"\u003e\n \u003cp\u003e37.5 (15)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"100%\" colspan=\"4\" valign=\"top\"\u003e\n \u003cp\u003eEducation [\u003cem\u003e% (n)\u003c/em\u003e]\u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"36.10648918469218%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;Primary education\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"26.788685524126457%\" valign=\"top\"\u003e\n \u003cp\u003e22.5 (9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.124792013311147%\" valign=\"top\"\u003e\n \u003cp\u003e37.5 (15)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.980033277870216%\" rowspan=\"4\"\u003e\n \u003cp\u003e0.41\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"41.02079395085066%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;Completed SSC\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.434782608695652%\" valign=\"top\"\u003e\n \u003cp\u003e32.5 (13)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"28.544423440453688%\" valign=\"top\"\u003e\n \u003cp\u003e22.5 (9)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"41.02079395085066%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;Completed HSC\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.434782608695652%\" valign=\"top\"\u003e\n \u003cp\u003e22.5 (9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"28.544423440453688%\" valign=\"top\"\u003e\n \u003cp\u003e22.5 (9)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"41.02079395085066%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;Bachelor and above degree\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.434782608695652%\" valign=\"top\"\u003e\n \u003cp\u003e22.5 (9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"28.544423440453688%\" valign=\"top\"\u003e\n \u003cp\u003e17.5 (7)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"100%\" colspan=\"4\" valign=\"top\"\u003e\n \u003cp\u003eLiving area [\u003cem\u003e% (n)\u003c/em\u003e]\u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"36.10648918469218%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;Urban\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"26.788685524126457%\" valign=\"top\"\u003e\n \u003cp\u003e27.5 (11)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.124792013311147%\" valign=\"top\"\u003e\n \u003cp\u003e42.5 (17)\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.980033277870216%\" rowspan=\"3\"\u003e\n \u003cp\u003e0.09\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"41.02079395085066%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;Rural\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.434782608695652%\" valign=\"top\"\u003e\n \u003cp\u003e47.5 (19)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"28.544423440453688%\" valign=\"top\"\u003e\n \u003cp\u003e37.5 (15)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"41.02079395085066%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; Semi-urban\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.434782608695652%\" valign=\"top\"\u003e\n \u003cp\u003e25.0 (10)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"28.544423440453688%\" valign=\"top\"\u003e\n \u003cp\u003e20.0 (8)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"100%\" colspan=\"4\" valign=\"top\"\u003e\n \u003cp\u003eOccupation [\u003cem\u003e% (n)\u003c/em\u003e]\u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"36.10648918469218%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;Day labourer\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"26.788685524126457%\" valign=\"top\"\u003e\n \u003cp\u003e15.0 (6)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.124792013311147%\" valign=\"top\"\u003e\n \u003cp\u003e22.5 (9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.980033277870216%\" rowspan=\"4\"\u003e\n \u003cp\u003e0.72\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"41.02079395085066%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;Service holder\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.434782608695652%\" valign=\"top\"\u003e\n \u003cp\u003e27.5 (11)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"28.544423440453688%\" valign=\"top\"\u003e\n \u003cp\u003e25.0 (10)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"41.02079395085066%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; Businessman\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.434782608695652%\" valign=\"top\"\u003e\n \u003cp\u003e22.5 (9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"28.544423440453688%\" valign=\"top\"\u003e\n \u003cp\u003e17.5 (7)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"41.02079395085066%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;Housewife\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.434782608695652%\" valign=\"top\"\u003e\n \u003cp\u003e35.0 (14)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"28.544423440453688%\" valign=\"top\"\u003e\n \u003cp\u003e35.0 (14)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"100%\" colspan=\"4\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eClinical characteristics\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"100%\" colspan=\"4\" valign=\"top\"\u003e\n \u003cp\u003eHistory of trauma [\u003cem\u003e% (n)\u003c/em\u003e]\u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"36.10648918469218%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;Over use trauma\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"26.788685524126457%\" valign=\"top\"\u003e\n \u003cp\u003e30.0 (12)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.124792013311147%\" valign=\"top\"\u003e\n \u003cp\u003e32.5 (13)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.980033277870216%\" rowspan=\"3\"\u003e\n \u003cp\u003e0.51\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"41.02079395085066%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;Direct trauma\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.434782608695652%\" valign=\"top\"\u003e\n \u003cp\u003e60.0 (24)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"28.544423440453688%\" valign=\"top\"\u003e\n \u003cp\u003e62.5 (25)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"41.02079395085066%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; Psychological trauma\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.434782608695652%\" valign=\"top\"\u003e\n \u003cp\u003e10.0 (4)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"28.544423440453688%\" valign=\"top\"\u003e\n \u003cp\u003e5.0 (2)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"100%\" colspan=\"4\" valign=\"top\"\u003e\n \u003cp\u003eChronic illness [\u003cem\u003e% (n)\u003c/em\u003e]\u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"36.10648918469218%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;Diabetic Mellitus\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"26.788685524126457%\" valign=\"top\"\u003e\n \u003cp\u003e17.5 (7)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.124792013311147%\" valign=\"top\"\u003e\n \u003cp\u003e20.0 (8)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.980033277870216%\" rowspan=\"4\"\u003e\n \u003cp\u003e0.46\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"41.02079395085066%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; Hypertension\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.434782608695652%\" valign=\"top\"\u003e\n \u003cp\u003e47.5 (19)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"28.544423440453688%\" valign=\"top\"\u003e\n \u003cp\u003e45.0 (18)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"41.02079395085066%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;Heart disease\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.434782608695652%\" valign=\"top\"\u003e\n \u003cp\u003e20.0 (8)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"28.544423440453688%\" valign=\"top\"\u003e\n \u003cp\u003e12.5 (5)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"41.02079395085066%\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;Obesity\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"30.434782608695652%\" valign=\"top\"\u003e\n \u003cp\u003e15.0 (6)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"28.544423440453688%\" valign=\"top\"\u003e\n \u003cp\u003e22.5 (9)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003eNote: IQR, Inter quartile range; SSC, Secondary School Certificate; HSC, Higher Secondary Certificate; \u003csup\u003ea\u0026nbsp;\u003c/sup\u003eMann-Whitney U test and \u003csup\u003eb\u0026nbsp;\u003c/sup\u003ePearson Chi-square test; \u003csup\u003e*\u003c/sup\u003ebaseline compatible\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eTable 2\u0026nbsp;\u003c/strong\u003eBaseline and after-treatment status of pain severity by Numeric Pain Rating Scale (NPRS)\u003c/p\u003e\n\u003ctable border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd width=\"16.80532445923461%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eOutcome group\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"23.12811980033278%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eBaseline\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"20.133111480865225%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eAfter\u003c/strong\u003e\u003c/p\u003e\n \u003cp\u003e\u003cstrong\u003etreatment\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"20.133111480865225%\" colspan=\"3\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eWithin group change scores\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"19.800332778702163%\" colspan=\"3\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eBetween group change scores\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"100%\" colspan=\"9\"\u003e\n \u003cp\u003e\u003cem\u003ePain at resting position\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"16.80532445923461%\" rowspan=\"2\" valign=\"top\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003cp\u003eExperimental\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"23.12811980033278%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eMedian (IQR)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"20.133111480865225%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eMedian (IQR)IQR\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"10.8153078202995%\" colspan=\"2\"\u003e\n \u003cp\u003e\u003cstrong\u003eZ\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.317803660565724%\"\u003e\n \u003cp\u003e\u003cstrong\u003ep\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.484193011647255%\"\u003e\n \u003cp\u003e\u003cstrong\u003eZ\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"10.316139767054908%\" colspan=\"2\"\u003e\n \u003cp\u003e\u003cstrong\u003ep\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"27.8%\"\u003e\n \u003cp\u003e5 (3 to 6)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"24.2%\"\u003e\n \u003cp\u003e1 (0 to 2)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13%\" colspan=\"2\"\u003e\n \u003cp\u003e5.544\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.2%\"\u003e\n \u003cp\u003e0.001\u003csup\u003e*\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.4%\" rowspan=\"2\"\u003e\n \u003cp\u003e6.052\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.4%\" colspan=\"2\" rowspan=\"2\"\u003e\n \u003cp\u003e0.0001\u003csup\u003e***\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"20.95435684647303%\" valign=\"top\"\u003e\n \u003cp\u003eControl\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"28.83817427385892%\" valign=\"top\"\u003e\n \u003cp\u003e6 (3 to 6)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.103734439834025%\" valign=\"top\"\u003e\n \u003cp\u003e2 (2 to 3)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.485477178423237%\" colspan=\"2\"\u003e\n \u003cp\u003e5.047\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.618257261410788%\"\u003e\n \u003cp\u003e0.001\u003csup\u003e*\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"100%\" colspan=\"9\"\u003e\n \u003cp\u003e\u003cem\u003ePain during rising arm sideways\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"16.777408637873755%\"\u003e\n \u003cp\u003eExperimental\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"23.089700996677742%\"\u003e\n \u003cp\u003e9 (8 to 9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"20.099667774086377%\"\u003e\n \u003cp\u003e2 (1 to 2)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"10.631229235880399%\"\u003e\n \u003cp\u003e5.489\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.46843853820598%\" colspan=\"2\"\u003e\n \u003cp\u003e0.001\u003csup\u003e*\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.800664451827242%\" colspan=\"2\" rowspan=\"2\"\u003e\n \u003cp\u003e7.518\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"10.132890365448505%\" rowspan=\"2\"\u003e\n \u003cp\u003e0.0001\u003csup\u003e***\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"20.95435684647303%\" valign=\"top\"\u003e\n \u003cp\u003eControl\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"28.83817427385892%\"\u003e\n \u003cp\u003e8 (8 to 9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.103734439834025%\"\u003e\n \u003cp\u003e5 (5 to 6)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.278008298755188%\"\u003e\n \u003cp\u003e5.358\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.825726141078839%\" colspan=\"2\"\u003e\n \u003cp\u003e0.001\u003csup\u003e*\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"100%\" colspan=\"9\"\u003e\n \u003cp\u003e\u003cem\u003ePain during combing hair\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"16.777408637873755%\"\u003e\n \u003cp\u003eExperimental\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"23.089700996677742%\"\u003e\n \u003cp\u003e9 (8 to 9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"20.099667774086377%\"\u003e\n \u003cp\u003e2 (1 to 3)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"10.631229235880399%\"\u003e\n \u003cp\u003e5.542\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.46843853820598%\" colspan=\"2\"\u003e\n \u003cp\u003e0.001\u003csup\u003e*\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.800664451827242%\" colspan=\"2\" rowspan=\"2\"\u003e\n \u003cp\u003e4.990\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"10.132890365448505%\" rowspan=\"2\"\u003e\n \u003cp\u003e0.0001\u003csup\u003e***\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"20.95435684647303%\" valign=\"top\"\u003e\n \u003cp\u003eControl\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"28.83817427385892%\"\u003e\n \u003cp\u003e9 (7 to 9)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.103734439834025%\"\u003e\n \u003cp\u003e5 (2 to 5)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.278008298755188%\"\u003e\n \u003cp\u003e5.256\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.825726141078839%\" colspan=\"2\"\u003e\n \u003cp\u003e0.001\u003csup\u003e*\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"16.422764227642276%\"\u003e\u003cbr\u003e\u003c/td\u003e\n \u003ctd width=\"22.601626016260163%\"\u003e\u003cbr\u003e\u003c/td\u003e\n \u003ctd width=\"19.67479674796748%\"\u003e\u003cbr\u003e\u003c/td\u003e\n \u003ctd width=\"10.40650406504065%\"\u003e\u003cbr\u003e\u003c/td\u003e\n \u003ctd width=\"0.3252032520325203%\"\u003e\u003cbr\u003e\u003c/td\u003e\n \u003ctd width=\"9.105691056910569%\"\u003e\u003cbr\u003e\u003c/td\u003e\n \u003ctd width=\"9.268292682926829%\"\u003e\u003cbr\u003e\u003c/td\u003e\n \u003ctd width=\"0.16260162601626016%\"\u003e\u003cbr\u003e\u003c/td\u003e\n \u003ctd width=\"12.032520325203253%\"\u003e\u003cbr\u003e\u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003eNote: \u003csup\u003e***\u003c/sup\u003eSignificant at 95% Confidence Level; Within-group analysis: Wilcoxon rank test; between-group analysis: Mann-Whitney U test\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eTable 3\u003c/strong\u003e Baseline and after-treatment scores for pain, disability and total SPADI score by using Shoulder Pain and Disability Index (SPADI)\u003c/p\u003e\n\u003ctable border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd width=\"16.80532445923461%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eOutcome group\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"23.12811980033278%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eBaseline\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"20.133111480865225%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eAfter\u003c/strong\u003e\u003c/p\u003e\n \u003cp\u003e\u003cstrong\u003etreatment\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"20.133111480865225%\" colspan=\"3\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eWithin group change scores\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"19.800332778702163%\" colspan=\"3\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eBetween group change scores\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"100%\" colspan=\"9\"\u003e\n \u003cp\u003e\u003cem\u003ePain intensity\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"16.80532445923461%\" rowspan=\"2\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003cp\u003eExperimental\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"23.12811980033278%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eMedian (IQR)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"20.133111480865225%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eMedian (IQR)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"10.8153078202995%\" colspan=\"2\"\u003e\n \u003cp\u003e\u003cstrong\u003eZ\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.317803660565724%\"\u003e\n \u003cp\u003e\u003cstrong\u003ep\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.484193011647255%\"\u003e\n \u003cp\u003e\u003cstrong\u003eZ\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"10.316139767054908%\" colspan=\"2\"\u003e\n \u003cp\u003e\u003cstrong\u003ep\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"27.8%\"\u003e\n \u003cp\u003e72 (64.50 to 76)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"24.2%\"\u003e\n \u003cp\u003e26 (22 to 30)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13%\" colspan=\"2\"\u003e\n \u003cp\u003e5.519\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.2%\"\u003e\n \u003cp\u003e0.001\u003csup\u003e*\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.4%\" rowspan=\"2\"\u003e\n \u003cp\u003e7.648\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"12.4%\" colspan=\"2\" rowspan=\"2\"\u003e\n \u003cp\u003e0.0001\u003csup\u003e***\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"20.95435684647303%\"\u003e\n \u003cp\u003eControl\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"28.83817427385892%\" valign=\"top\"\u003e\n \u003cp\u003e75 (68.50 to 79.50)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.103734439834025%\" valign=\"top\"\u003e\n \u003cp\u003e50 (46 to 54)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.485477178423237%\" colspan=\"2\"\u003e\n \u003cp\u003e2.820\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.618257261410788%\"\u003e\n \u003cp\u003e0.032\u003csup\u003e*\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"100%\" colspan=\"9\"\u003e\n \u003cp\u003e\u003cem\u003eDisability level\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"16.777408637873755%\"\u003e\n \u003cp\u003eExperimental\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"23.089700996677742%\"\u003e\n \u003cp\u003e64.50 (60 to 69.75)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"20.099667774086377%\"\u003e\n \u003cp\u003e22 (18 to 25)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"10.631229235880399%\"\u003e\n \u003cp\u003e5.516\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.46843853820598%\" colspan=\"2\"\u003e\n \u003cp\u003e0.001\u003csup\u003e*\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.800664451827242%\" colspan=\"2\" rowspan=\"2\"\u003e\n \u003cp\u003e7.696\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"10.132890365448505%\" rowspan=\"2\"\u003e\n \u003cp\u003e0.0001\u003csup\u003e***\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"20.95435684647303%\" valign=\"top\"\u003e\n \u003cp\u003eControl\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"28.83817427385892%\"\u003e\n \u003cp\u003e67 (62 to 74)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.103734439834025%\"\u003e\n \u003cp\u003e44.50 (41 to 48)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.278008298755188%\"\u003e\n \u003cp\u003e2.685\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.825726141078839%\" colspan=\"2\"\u003e\n \u003cp\u003e0.028\u003csup\u003e*\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"100%\" colspan=\"9\"\u003e\n \u003cp\u003e\u003cem\u003eTotal Score\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"16.777408637873755%\"\u003e\n \u003cp\u003eExperimental\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"23.089700996677742%\"\u003e\n \u003cp\u003e67 (62 to 71)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"20.099667774086377%\"\u003e\n \u003cp\u003e23 (20 to 27)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"10.631229235880399%\"\u003e\n \u003cp\u003e5.517\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.46843853820598%\" colspan=\"2\"\u003e\n \u003cp\u003e0.001\u003csup\u003e*\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"9.800664451827242%\" colspan=\"2\" rowspan=\"2\"\u003e\n \u003cp\u003e7.707\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"10.132890365448505%\" rowspan=\"2\"\u003e\n \u003cp\u003e0.0001\u003csup\u003e***\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"20.95435684647303%\" valign=\"top\"\u003e\n \u003cp\u003eControl\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"28.83817427385892%\"\u003e\n \u003cp\u003e70 (64.75 to 75)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"25.103734439834025%\"\u003e\n \u003cp\u003e46.50 (43 to 49)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.278008298755188%\"\u003e\n \u003cp\u003e2.515\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"11.825726141078839%\" colspan=\"2\"\u003e\n \u003cp\u003e0.021\u003csup\u003e*\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003eNote: \u003csup\u003e***\u003c/sup\u003eSignificant at 95% Confidence Level; Within-group analysis: Wilcoxon rank test; between-group analysis: Mann-Whitney U test\u003c/p\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Adhesive Capsulitis, Proprioceptive Neuromuscular Facilitation (PNF) Pattern, Upper Extremity, Standard Physiotherapy","lastPublishedDoi":"10.21203/rs.3.rs-4843786/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4843786/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003ch2\u003eBackground\u003c/h2\u003e \u003cp\u003eAdhesive capsulitis (AC) is a progressive inflammatory condition of the shoulder that causes functional limitations and leads to long-term disability. The study aimed to elicit the effectiveness of Proprioceptive neuromuscular facilitation (PNF) compared to standard physiotherapy approaches on AC.\u003c/p\u003e\u003ch2\u003eMethods\u003c/h2\u003e \u003cp\u003eAn assessor-blinded single-centre Randomized control trial (RCT) was carried out on 80 AC patients between May and December 2023 in Bangladesh. Random assigned and concealed allocated patients were recruited equally (n\u0026thinsp;=\u0026thinsp;40) to each PNF and conventional capsular stretching group. The experimental group received a PNF approach, and the control group received capsular stretching to the affected shoulder for 24 sessions in 6 weeks. Both groups received electrical modalities as standard treatment. The primary outcome was pain measured by the Numeric pain rating scale (NPRS) and range of motion in a universal goniometer. The secondary outcome was functional limitation measured by the Shoulder pain and disability index (SPADI). As per the distribution of data, non-parametric tests were employed to analyse the superiority between and within groups with intention-to-treat analysis.\u003c/p\u003e\u003ch2\u003eResults\u003c/h2\u003e \u003cp\u003eBaseline compatibility was noted in all the key variables (P\u0026thinsp;\u0026gt;\u0026thinsp;0.05). Both PNF and capsular stretching had a decrease in pain compared to baseline in all capsular pattern positions (P\u0026thinsp;\u0026lt;\u0026thinsp;0.001), and PNF had more significant improvement compared to control (P\u0026thinsp;\u0026lt;\u0026thinsp;0.001). Both groups had equal improvement in shoulder ROM (P\u0026thinsp;\u0026lt;\u0026thinsp;0.001) except abduction (P\u0026thinsp;\u0026lt;\u0026thinsp;0.05). Both groups had improvements in disability (P\u0026thinsp;\u0026lt;\u0026thinsp;0.05), and PNF had statistical superiority of improvement (P\u0026thinsp;\u0026lt;\u0026thinsp;0.001).\u003c/p\u003e\u003ch2\u003eConclusions\u003c/h2\u003e \u003cp\u003eThe findings of this study support the potential of PNF intervention for 6 weeks as a treatment for shoulder Adhesive capsulitis, showing improvements in pain, ROM, and functional disability. However, further multicentre trials with a follow-up design are needed to fully understand the superiority of PNF on shoulder AC, encouraging continued engagement in this area of research.\u003c/p\u003e\u003ch2\u003eTrial registration:\u003c/h2\u003e \u003cp\u003eThe Australian New Zealand Clinical Trial Registry (\u003cspan class=\"ExternalRef\"\u003e\u003cspan class=\"RefSource\"\u003ehttp://www.anzctr.org.au\u003c/span\u003e\u003cspan address=\"http://www.anzctr.org.au\" targettype=\"URL\" class=\"RefTarget\"\u003e\u003c/span\u003e\u003c/span\u003e) (ACTRN12621001299897).\u003c/p\u003e","manuscriptTitle":"Effectiveness of Proprioceptive Neuromuscular Facilitation Pattern on Upper Extremity and Scapula in Patients with Adhesive Capsulitis: A single centre assessor-blinded Randomised controlled trial (RCT)","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-09-26 01:47:06","doi":"10.21203/rs.3.rs-4843786/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"2e539eb4-a4d0-4761-af72-0d850e452791","owner":[],"postedDate":"September 26th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2024-09-26T01:47:06+00:00","versionOfRecord":[],"versionCreatedAt":"2024-09-26 01:47:06","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-4843786","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4843786","identity":"rs-4843786","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}