Detection of macrophage migration inhibitory factor secretion of ectopic and eutopic endometrial cells cultured with progesterone

Aim: To evaluate the regulatory effects of progesterone on the secretion of migration inhibitory factor(MIF) by ectopic and eutopic endometrial cells in vitro. Methods: Eutopic and ectopic endometrial cells from 13 cases of endometriosis were cultured and exposed to progesterone(10 -5 mol/L,10 -7 mol/L, respectively). MIF secretion was analyzed in the culture supernatant by enzyme-linked immumosorbent assay (ELISA). Results: Progesterone could inhibit the MIF secretion in ectopic and eutopic endometrial cells. Level of MIF in ectopic group was significantly higher than that of eutopic group. The effects of down regulation were more obvious when the concentration of progesterone increased. Conclusion: Progesterone can inhibit the MIF secretion in ectopic and eutopic endometrial cells, especially to the ectopic endometrial cells.