Screening and Role of circRNAs in Vulvar Lichen Sclerosus

Objective: ：The aim of this study was to investigate the expression profile of circRNA in Vulvar Lichen Sclerosus, and to identify the underlying core genes of Vulvar Lichen Sclerosus.Methods：rRNA removal was used for sequencing, and mRNA, lncRNA and circRNA differentially expressed between 20 groups of VLS tissues and 20 groups of healthy female vulva skin tissues were screened. Bioinformatics analysis was used to analyze its potential function. Results: ： A total of 1545 differentially expressed mRNAs were assessed in VLS patients, of which 1541 were upregulated and 1004 were down-regulated; A total of 1453 differentially expressed lncRNAs were assessed, of which 812 were up-regulated and 641 were down-regulated. A total of 79 differentially expressed circRNAs were assessed, of which 54 were upregulated and 25 were down-regulated. The differential expression of CircRNA is closely related to biological processes and molecular functions. The differences in circRNA were mainly related to the "human T-cell leukemia virus 1 infection" signaling pathway and the "axon guidance" signaling pathway. Conclusion: ： This study identified the profile of abnormal regulation of circRNA in VLS. Biological informatics analysis showed that the dysregulation of circRNAs could be related to the pathogenesis and pathological process of VLS.