Investigating the Impact of Pillar Shape on Flexible Erythrocyte Separation through Deterministic Lateral Displacement

Investigating the Impact of Pillar Shape on Flexible Erythrocyte Separation through Deterministic Lateral Displacement | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Investigating the Impact of Pillar Shape on Flexible Erythrocyte Separation through Deterministic Lateral Displacement Jiangbo Wu, Wenyu Zhang, Xiaoze Du, Jie Liu, Yao lv, Yongqing He This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3905453/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract The deterministic lateral displacement (DLD) method has gradually moved beyond the separation of spherical rigid particles and has been successfully applied to the field of biological cells, especially to the separation of disc-shaped flexible erythrocytes. Different shapes of micropillars in the DLD device significantly affect the sorting performance of erythrocytes. We investigated the effect of the flow field in the gap of micropillars of different shapes (circular, triangular, I-shaped) on the behavior of erythrocyte tumbling motion. The simulation results showed that in the I-shaped pillar DLD device, compared with the circular and triangular pillars, the erythrocytes exhibited a stable and continuous flipping motion during the movement along the array offset direction, which enabled the effective separation size to be maintained at a high level. In addition, the formula for calculating the critical diameter of the I-shaped pillar DLD array for separating erythrocytes was obtained by fitting, which provides strong support for more efficient design of the I-shaped pillar DLD device dedicated to erythrocyte sorting. Flexible erythrocyte Pillar shape Tumbling movement Effective separation size Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3905453","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":269900152,"identity":"89565d61-ceee-4316-9e84-c8d30f7e6f68","order_by":0,"name":"Jiangbo Wu","email":"","orcid":"","institution":"Lanzhou University of Technology","correspondingAuthor":false,"prefix":"","firstName":"Jiangbo","middleName":"","lastName":"Wu","suffix":""},{"id":269900153,"identity":"99d493f4-31f4-44a1-849a-631b0550e19f","order_by":1,"name":"Wenyu Zhang","email":"","orcid":"","institution":"Lanzhou University of Technology","correspondingAuthor":false,"prefix":"","firstName":"Wenyu","middleName":"","lastName":"Zhang","suffix":""},{"id":269900154,"identity":"6e5d311a-fb39-4edf-8926-c4e42c08baef","order_by":2,"name":"Xiaoze Du","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAw0lEQVRIiWNgGAWjYDCCA0D8AcbhIVYL4wyStTDDVRKlhe9GjvFn2x12cuYSCYwP3rYxyJsT0iJ5Iy3BOPdMsrHljARmw7ltDIY7GwhoMbiRfCA5t+1A4oYbCWzSvG0MCQYHCGpJbDhs2XagHqiF/TeRWpIPNjO2HUgwANrCTJQWyTPPkhl725INd/Y8bJacc07CcAMhLXzHc4w//GyzkzdnTz744U2ZjTxBWxAuZGBsAFISxKoHaxkFo2AUjIJRgAMAALSJQGyFj31pAAAAAElFTkSuQmCC","orcid":"","institution":"Lanzhou University of Technology","correspondingAuthor":true,"prefix":"","firstName":"Xiaoze","middleName":"","lastName":"Du","suffix":""},{"id":269900155,"identity":"0213de08-c6e7-4f24-b44a-8a08fd6d9dc0","order_by":3,"name":"Jie Liu","email":"","orcid":"","institution":"Lanzhou University of Technology","correspondingAuthor":false,"prefix":"","firstName":"Jie","middleName":"","lastName":"Liu","suffix":""},{"id":269900156,"identity":"fd1fee43-2cb8-4927-9972-d10e879eda4c","order_by":4,"name":"Yao lv","email":"","orcid":"","institution":"Lanzhou University of Technology","correspondingAuthor":false,"prefix":"","firstName":"Yao","middleName":"","lastName":"lv","suffix":""},{"id":269900157,"identity":"5eed75fe-a528-4bd3-aa4e-2df2b949f5b2","order_by":5,"name":"Yongqing He","email":"","orcid":"","institution":"Chongqing Technology and Business University","correspondingAuthor":false,"prefix":"","firstName":"Yongqing","middleName":"","lastName":"He","suffix":""}],"badges":[],"createdAt":"2024-01-28 10:29:13","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-3905453/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-3905453/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":51032411,"identity":"890f143f-f49b-41fb-8c3e-7cb1be774eb9","added_by":"auto","created_at":"2024-02-13 02:59:57","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1077046,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-3905453/v1_covered_bbc695fb-b0e0-40d9-9db7-dbe6828f04ea.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Investigating the Impact of Pillar Shape on Flexible Erythrocyte Separation through Deterministic Lateral Displacement","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Flexible erythrocyte, Pillar shape, Tumbling movement, Effective separation size","lastPublishedDoi":"10.21203/rs.3.rs-3905453/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-3905453/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eThe deterministic lateral displacement (DLD) method has gradually moved beyond the separation of spherical rigid particles and has been successfully applied to the field of biological cells, especially to the separation of disc-shaped flexible erythrocytes. Different shapes of micropillars in the DLD device significantly affect the sorting performance of erythrocytes. We investigated the effect of the flow field in the gap of micropillars of different shapes (circular, triangular, I-shaped) on the behavior of erythrocyte tumbling motion. The simulation results showed that in the I-shaped pillar DLD device, compared with the circular and triangular pillars, the erythrocytes exhibited a stable and continuous flipping motion during the movement along the array offset direction, which enabled the effective separation size to be maintained at a high level. 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