Investigating DNA-Interaction and Anticancer Activity of new Cu +2 complex Coordinated with Dipicolinic Acid. Experimental and Computational Studies

Investigating DNA-Interaction and Anticancer Activity of new Cu +2 complex Coordinated with Dipicolinic Acid. Experimental and Computational Studies | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Investigating DNA-Interaction and Anticancer Activity of new Cu +2 complex Coordinated with Dipicolinic Acid. Experimental and Computational Studies Ameneh Heidari, Effat Dehghanian, Somaye Shahraki, Zohreh Razmara, and 4 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5739643/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract A complex formulized as [Cu(dmpn) 2 (OH 2 )][Cu 2 (DPA) 4 µ-Cu(dmpn) 2 ] ( 1 ), where dmpn is 2,2 ׳ -dimethyl-1,3-diaminepropane and DPA is dipicolinic acid (H 2 dipic), was synthesized and characterized by X-ray crystallography, conductivity measurement, thermogravimetric (TG) analysis and spectroscopic methods (IR, UV-Vis). The initial in vitro cytotoxic activity of complex ( 1 ) shows promising results compared to known anticancer drugs such as cisplatin against HCT116 colon cell lines. In a recent study, the mode of the interaction between DNA and complex ( 1 ) was explored using different techniques. The experiments reveal that the mechanism of fluorescence quenching is static and the characteristic of this interaction is exothermic. The apparent binding constant (K app = 1.9×10 3 M − 1 ) shows that the interaction between the cu(II) complex and DNA is probably non-intercalation. Based on other data, it can be suggested that hydrogen bonding, and van der Waals forces play a major role in this interaction. Molecular docking simulation supports the achieved experimental results. Likewise, complex ( 1 ) shows nuclease activity in the presence of H 2 O 2 and can cleavage structure of DNA. DNA-binding Cytotoxicity DNA-cleavage Dipicolinic acid Fluorescence spectroscopy Full Text Additional Declarations No competing interests reported. Supplementary Files Supplementary.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-5739643","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":398343747,"identity":"6055a5c7-8f8a-4240-ad98-231f45242424","order_by":0,"name":"Ameneh Heidari","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA00lEQVRIiWNgGAWjYBACNgYeBoYENiCDvQEqxExACz9cC88BIrVINvCA7WJgkEgg0mEG184efPCgzC6fT/KN4cMfDHbyDOy8D/BruZ2XbJBwLtmyTTrH2ECCIdmwgZndgICWHDOJxDZmAzZpIMOAgTmBgZkNv8Psb+eY/0hsqzdgkzxjBvROPWEtIFsYEtsOG7BJ8JhJHGA4TJQWY4mEc8cN2HjSig0bDI4bthGhxfDjj7JqA/n2wxsf/qiolufnP4ZfC7oJkDgaBaNgFIyCUUAhAADmYzcrItftkQAAAABJRU5ErkJggg==","orcid":"","institution":"University of Zabol","correspondingAuthor":true,"prefix":"","firstName":"Ameneh","middleName":"","lastName":"Heidari","suffix":""},{"id":398343748,"identity":"4fabbf9a-3480-4f37-ac99-4e9ad586dd24","order_by":1,"name":"Effat Dehghanian","email":"","orcid":"","institution":"Bu-Ali Sina University","correspondingAuthor":false,"prefix":"","firstName":"Effat","middleName":"","lastName":"Dehghanian","suffix":""},{"id":398343752,"identity":"05a6f630-d4eb-4c5d-a6ff-b2631434f543","order_by":2,"name":"Somaye Shahraki","email":"","orcid":"","institution":"University of Zabol","correspondingAuthor":false,"prefix":"","firstName":"Somaye","middleName":"","lastName":"Shahraki","suffix":""},{"id":398343754,"identity":"7e48a4a2-f16c-44a2-9dfb-144fc75ef19b","order_by":3,"name":"Zohreh Razmara","email":"","orcid":"","institution":"University of Zabol","correspondingAuthor":false,"prefix":"","firstName":"Zohreh","middleName":"","lastName":"Razmara","suffix":""},{"id":398343755,"identity":"50f20baa-7f52-4c90-852e-eae17df87185","order_by":4,"name":"Mostafa Heidari Majd","email":"","orcid":"","institution":"Zabol University of Medical Sciences","correspondingAuthor":false,"prefix":"","firstName":"Mostafa","middleName":"Heidari","lastName":"Majd","suffix":""},{"id":398343756,"identity":"b3fac301-8143-4dd0-b39c-91da198d82df","order_by":5,"name":"Hamid Ahmar","email":"","orcid":"","institution":"University of Zabol","correspondingAuthor":false,"prefix":"","firstName":"Hamid","middleName":"","lastName":"Ahmar","suffix":""},{"id":398343759,"identity":"6b963a1c-765b-4727-a0d0-cc84df057b2d","order_by":6,"name":"Mansoureh Rakhshanipour","email":"","orcid":"","institution":"University of Zabol","correspondingAuthor":false,"prefix":"","firstName":"Mansoureh","middleName":"","lastName":"Rakhshanipour","suffix":""},{"id":398343760,"identity":"1b57d1be-b57f-4f06-a035-41db124a86de","order_by":7,"name":"Srikanta Goswami","email":"","orcid":"","institution":"Institute of Physics","correspondingAuthor":false,"prefix":"","firstName":"Srikanta","middleName":"","lastName":"Goswami","suffix":""}],"badges":[],"createdAt":"2024-12-31 06:23:20","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-5739643/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-5739643/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":74345122,"identity":"9170ac89-9d17-4229-91ed-e12a0450cfb4","added_by":"auto","created_at":"2025-01-21 09:39:31","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":944691,"visible":true,"origin":"","legend":"","description":"","filename":"MANUSCRIPT.pdf","url":"https://assets-eu.researchsquare.com/files/rs-5739643/v1_covered_9bfda3b0-3140-4d5c-a693-0c5723df2573.pdf"},{"id":73312086,"identity":"7011a4e9-b685-4d5b-8449-84a35b17bbcc","added_by":"auto","created_at":"2025-01-08 18:26:25","extension":"docx","order_by":0,"title":"","display":"","copyAsset":false,"role":"supplement","size":230899,"visible":true,"origin":"","legend":"","description":"","filename":"Supplementary.docx","url":"https://assets-eu.researchsquare.com/files/rs-5739643/v1/b4f288de89629499efc1ed3a.docx"}],"financialInterests":"No competing interests reported.","formattedTitle":"Investigating DNA-Interaction and Anticancer Activity of new Cu +2 complex Coordinated with Dipicolinic Acid. 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The initial \u003cem\u003ein vitro\u003c/em\u003e cytotoxic activity of complex (\u003cb\u003e1\u003c/b\u003e) shows promising results compared to known anticancer drugs such as cisplatin against HCT116 colon cell lines. In a recent study, the mode of the interaction between DNA and complex (\u003cb\u003e1\u003c/b\u003e) was explored using different techniques. The experiments reveal that the mechanism of fluorescence quenching is static and the characteristic of this interaction is exothermic. The apparent binding constant (K\u003csub\u003eapp\u003c/sub\u003e = 1.9\u0026times;10\u003csup\u003e3\u003c/sup\u003e M\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e) shows that the interaction between the cu(II) complex and DNA is probably non-intercalation. Based on other data, it can be suggested that hydrogen bonding, and van der Waals forces play a major role in this interaction. Molecular docking simulation supports the achieved experimental results. Likewise, complex (\u003cb\u003e1\u003c/b\u003e) shows nuclease activity in the presence of H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e and can cleavage structure of DNA.\u003c/p\u003e","manuscriptTitle":"Investigating DNA-Interaction and Anticancer Activity of new Cu +2 complex Coordinated with Dipicolinic Acid. 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