Additional file 3: of Endometrium and endometriosis tissue mitochondrial energy metabolism in a nonhuman primate model

Figure S3. Targeted metabolomics experimental design and statistical analysis. Eight clinically healthy and 17 nonhuman primates (NHP) with endometriosis were identified through the Wake Forest Primate Center Comprehensive Animal Record System (CARS). Of the 17 NHPs with endometriosis, 12 had available endometrium for analysis. Endometrium from clinically healthy animals was designated nEM. Endometrium and endometriosis tissue from NHPs with endometriosis were EM and EMO, respectively. Targeted metabolomics was performed by the Wake Forest Proteomics and Metabolomics Shared Resource facility. Twenty-nine metabolites were identified using LC-MS/MS. Statistical analyses of tissue metabolite concentrations were completed using MetaboAnalyst 4.0. Metabolite concentrations were log-transformed and analyzed by partial least squares (PLS) and principle component (PCA) analyses. Significant metabolites determined with PLS and PCA were further analyzed using one-way analysis of variance (ANOVA) and GraphPad statistical software. Finally, metabolites that proved significant with both PLS/PCA and ANOVA were entered into pathway analysis algorithms associated with MetaboAnalyst 4.0 to identify relevant pathways. (DOCX 108 kb)