Rapid Detection ofCandida aurisUsing a Loop-Mediated Isothermal Amplification (LAMP) method

Background Candida auris (C. auris) can cause nosocomial transmission in susceptible hosts. This study aimed to use the Loop-Mediated Isothermal Amplification (LAMP) technique as a novel detection technique for C. auris and compare the efficacy with PCR

This study was performed on C. auris fungal isolates as positive samples and five different standard Candida species as negative control samples. The sensitivity and specificity were determined using different C. auris DNA concentrations. LAMP products were identified by electrophoresis, turbidity, and colorimetric methods. Finally, the products were sequenced

The LOD (limit of detection) of the LAMP was 10 times lower than the PCR reaction. LAMP and PCR correctly identified C. auris with 100% specificity. The nBLAST results showed the similarity between the sequences of the PCR products and Gene Bank sequences, confirming the specific amplification of target gene in C. auris genome by LAMP and PCR methods.

The reproducibility and reliability of the LAMP reaction in differentiating C. auris from closely related species were confirmed. The developed LAMP technique holds promise for testing the clinical samples for quick detection of C. auris with excellent accuracy. Competing Interest Statement The authors have declared no competing interest. Funding Statement Tabriz University of Medical Sciences Author Declarations I confirm all relevant ethical guidelines have been followed, and any necessary IRB and/or ethics committee approvals have been obtained. Yes I confirm that all necessary patient/participant consent has been obtained and the appropriate institutional forms have been archived, and that any patient/participant/sample identifiers included were not known to anyone (e.g., hospital staff, patients or participants themselves) outside the research group so cannot be used to identify individuals. Yes I understand that all clinical trials and any other prospective interventional studies must be registered with an ICMJE-approved registry, such as ClinicalTrials.gov. I confirm that any such study reported in the manuscript has been registered and the trial registration ID is provided (note: if posting a prospective study registered retrospectively, please provide a statement in the trial ID field explaining why the study was not registered in advance). Yes I have followed all appropriate research reporting guidelines, such as any relevant EQUATOR Network research reporting checklist(s) and other pertinent material, if applicable. Yes Data Availability All data produced in the present study are available upon reasonable request to the authors