Expression change of LIF in endometrium after electrosurgery on endometriotic niduses

Objective:To understand the meaning and mechanism of electric coagulation on endometric niduses in treatment of infertility by observing the expression change of LIF in endometrium. Methods:Fifty cases of infertility women with endometriosis in AFS stageⅠ～Ⅱ were enrolled as study group, 50 cases of infertility women without endometriosis but with husband abnormal sperma were enrolled as control group. Both groups were performed laparoscopy, endometric niduses were removed by electric coagulation as completely as possible. Immunohistochemical localization of leukaemia inhibitory factor (LIF) protein and In-situ localization of leukaemia inhibitory factor (LIF) mRNA in endometrium were performed by medical image analytical system during 19-24 days of last one menstrual cycle before laparoscopy and the first and third menstrual cycle after laparoscopy. Results: The change of In-situ localization of LIF mRNA in endometrium: the level of LIF mRNA were 3.0±0.6 before laparoscopy, 4.1±0.9 one month and 4.8±1.1 three month after laparoscopy in study group; in control group, the level was 5.9±1.2、6.1±1.1、6.3±1.0 separately. There was significant difference between two groups and significant change after laparoscopy in study group(P0.05). The change of immunohistochemical localization of LIF protein in endometrium: the level of LIF protein were 9.0±1.6 before laparoscopy, 11.6±2.4 one month and 13.6±3.4 three month after laparoscopy in study group; in control group, the level was 17.7±3.4、17.8±3.3、18.0±3.3 separately. There was significant difference between two groups and significant change after laparoscopy in study group(P0.05).Conclusion:There was defectiveness in expression of LIF in endometrium of infertility women with endometriosis. There was some improvement in expression of LIF in endometrium after electric coagulation on endometric niduses, but could not recover to normal condition.