Antimalarial effect of ethanol extract of Parinari curatellifolia stem-bark in Plasmodium berghei-NK 65 infected mice.

Antimalarial effect of ethanol extract of Parinari curatellifolia stem-bark in Plasmodium berghei-NK 65 infected mice. | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Antimalarial effect of ethanol extract of Parinari curatellifolia stem-bark in Plasmodium berghei -NK 65 infected mice. Lomi Oruchukwu Michael, Parker Elijah Joshua, Christian Chijioke Amah, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3586444/v2 This work is licensed under a CC BY 4.0 License Status: Posted Version 2 posted You are reading this latest preprint version Show more versions Abstract Background: The use of Parinari curatellifolia for the management of malaria by Africans without scientific validation, prompted this study, to investigate the antimalarial effect of ethanol extract of Parinari curatellifolia stem bark (EEPCSB) in Plasmodium berghei (NK-65) -infected mice. Method: Thirty mice (16 -21 g) were grouped into six of five mice each. Group 1 (uninfected) served as normal control while groups 2-6 were infected . Group 2 (positive control) was untreated while group 3 (standard control) received 20/120 mg/kg b.w. of arthemeter/lumefantrine, groups 4-6 received 200, 400 and 600 mg/kg b.w. of EEPCSB respectively. The treatment lasted for four consecutive days. Parasitemia, heamatological and biochemical tests were carried out using established methods. The obtained data were analysed using one-way analysis of variance (ANOVA). Result: The extract significantly (p < 0.05) suppressed the parasitemia, and produced significant (p < 0.05) increase in the PCV, RBC, and Hgb and a significant (p < 0.05) decrease in the WBC of groups 4-6 compared to the positive control. The liver function marker enzymes - AST, ALT, ALP, and total bilirubin were all significantly (p < 0.05) reduced in the treated animals, while there was a significant (p < 0.05) decrease in the total cholesterol, triacylglycerol and LDL, and a significant (p < 0.05) increase in the HDL of the infected animals compared to the positive control. While there was a significant (p < 0.05) reduction in lipid peroxidation marker MDA in the treated groups, the activities of the SOD and catalase, significantly (p < 0.05) increased compared to the positive control. The GSH concentration reduced significantly (p < 0.05) in the treated groups compared to the untreated group. Conclusion: The extract showed excellent antimalarial effect by reducing parasitemia and reversing haematological and biochemical aberrations in Plasmodium berghei -infected mice as demonstrated in this study. Therefore, Parinari curatellifolia stem bark could be a source of lead compound for a novel and effective antimalarial drug. Parinari curatellifolia antimalarial parasitemia Plasmodium berghei ethanol extract Mobola plum Introduction Malaria has continued to be a major public health problem despite efforts at controlling it. It is widespread throughout tropical and subtropical regions of the world, and Africa carries a disproportionately high share of the global malaria burden, both in terms of total malaria morbidity and mortality. World Health Organization (WHO) reported that, in 2021, 247 million cases of malaria and 619,000 deaths in 87 malaria endemic countries globally were recorded. Africa accounted for 95% of the total cases and deaths. Ninety-six percent (96%) of the cases and deaths took place in 29 of the 87 malaria endemic countries (WHO, 2022). According to the 2022 World Malaria Report, Nigeria had the highest number of global malaria cases (27 % of global malaria cases) in 2021 and accounted for the highest number of deaths (31 % of global malaria deaths). Malaria is caused by Plasmodium parasites that are transmitted to humans through the bites of infected female Anopheles mosquitoes. Plasmodium falciparum, Plasmodium malariae, Plasmodium ovale curtisi, Plasmodium ovale wallicker, Plasmodium vivax, and Plasmodium knowlesi , are the five species of the parasite that infects humans, with Plasmodium falciparum being the deadliest (Milner, 2018; Prabhu et al ., 2021). Plasmodium has a complex life cycle involving both sexual and asexual replications in both the insect and human hosts respectively (Garrido-Cardenas et al ., 2019). During a blood meal, the female Anopheles mosquito transmits the sporozoites to humans. The sporozoites travels through the blood capillaries to the liver where it replicates asexually in the hepatocyte and mature into the liver schizont containing many merozoites, which are released into the blood stream. The merozoites invade the red blood cells and undergo intra-erythrocytic asexual replications and mature into the erythrocytic schizonts. The cycle continues, resulting in acute and chronic parasitemia. Some parasites commit to producing gametocytes, which are picked up by the mosquito during a blood meal. The sporozoites which are transmitted to another person upon blood meal, are produced through the sexual fusion of the gametocytes (Venugopal et al ., 2020). The classic malaria paroxysms, which are marked by recurrent fevers and chills, are caused by the release of merozoites from infected red blood cells (iRBC) (Oliver et al ., 2014). Depending on the plasmodium species, the host's inherent and acquired immunity, and the selection of an appropriate and timely course of therapy, the clinical signs of malaria might differ significantly. Consequently, asymptomatic, uncomplicated and severe are three types of malaria seen in humans (Garrido-Cardenas et al ., 2019). Malaria initially manifests as flu-like symptoms, including fever, chills, headaches, joint pains, nausea, vomiting, coughing, chest discomfort, myalgia, and weakness. Few patients go on to develop severe malaria which leads to coma, acute renal failure, severe anemia, acidosis, pulmonary edema and hypoglycemia (Su et al ., 2019). Quinine has been the foremost drug for treating severe malaria for more than 200 years. It was isolated from the bark of Cinchona species in 1820. Despite the significant limitations of quinine, it has nonetheless had long history of efficacy. The case-fatality rate in severe malaria, when treated promptly with quinine exceeds 20% (Li & Weina, 2010). Chloroquine, which is another important antimalarial drug, was synthesized in 1940. It remained the first line of treatment for uncomplicated malaria, until it was recently replaced with artemisinin-based therapy due to widespread resistance to chloroquine especially by Plasmodium falciparum (Saxena et al ., 2003). Based on the recommendations of WHO, artemisinin combination therapy (ACT) was introduced in twenty African countries between 2001 and 2004, for treatment of malaria. Currently, ACT is the first-line treatment for uncomplicated falciparum malaria in Africa, Asia and South America (Maiga et al ., 2021). Nevertheless, as was initially noted in Cambodia in 2006 and expanding to other regions of South-east Asia and Africa, resistance to artemisinin is emerging (Maiga et al ., 2021; Tajbakhsh et al ., 2021), has necessitated the search for a novel antimalarial drug. In several regions of the world, particularly in malaria-endemic countries like Africa, numerous plants can be used to cure malaria. A good number of these plants have been documented in various ethnobotanical surveys of plants used in treating malaria (Iyamah and Idu, 2015; Ungogo et al ., 2020; Enebeli-Ekwutoziam et al ., 2021). As there has been an increasing resistance to the front-line antimalarial drugs, the quest to discover and develop new anti-malarial drugs has led to various in vitro and in vivo studies of plants species used ethno-medically in treating malaria in Africa. A considerable number of plant species have shown appreciable anti-plasmodial and antimalarial activity, however, not much success has been recorded in developing a new antimalarial drug, as only one clinical trial with Cochlospermum planchonii has been carried out till date (Tajbakhsh et al ., 2021). Parinari curatellifolia Planch ex Benth (Chrysobalanaceae), commonly known as Mbola or Mobola plum, is a large ever green, spreading tree up to 22 metres tall with a single bare stem and a dense, roundish to mushroom shaped crown. The plant's therapeutic qualities make it extremely valuable. In ethnomedicine, it is used to cure diabetes, fractures, wounds, cuts, and sores as well as fevers, pneumonia, and toothaches (Halilu et al ., 2008; Ogbonnia et al ., 2009). Various in vitro anti-malarial studies of root, root bark, and stem bark extracts of the plant using various solvents have been carried out with reports of good activity (Omoniwa et al ., 2021; Tajbakhsh et al ., 2021). However, there is no reported in vivo study on the anti-plasmodial/antimalarial activity of this plant till date. Materials and methods 2.1 Materials 2.1.1 Plant Materials Fresh stem-bark of Parinari curatellifolia was collected from Agu-orba in Udenu Local Government Area of Enugu State, Nigeria. The stem-bark was identified and authenticated by a taxonomist of the International Centre for Ethnomedicine and Drug Development, Nsukka Enugu State, Nigeria. It was assigned the voucher number InterCEDD/16291 and deposited in the Centre’s herbarium. 2.1.2 Animals Forty-eight (48) Albino mice of average body weight of 20 ± 5g mice were used for this study. The animals were purchased from the Animal House of the Faculty of Veterinary Medicine, University of Nigeria, Nsukka. The animals were kept under standard laboratory condition for one week prior to the commencement of the experiment, with a 12-hour light and dark cycle and maintained on a regular feed (commercial chicken grower’s mash) and water ad libitum . They were given adequate care throughout the period of the experiment in accordance with the ethical rules and recommendations of the University of Nigeria Ethical committee on the care and use of laboratory animals. 2.1.3 Parasite Chloroquine-sensitive Plasmodium berghei NK 65 strain which was used for the study was obtained from blood samples collected from infected donor mice from the faculty of Veterinary Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria. The blood sample was diluted with normal saline before inoculation into the animals. 2.1.4 Equipment and Instruments The equipment used for this study was obtained from the Department of Biochemistry, the Department of Veterinary Medicine of the University of Nigeria Nsukka, and other scientific shops in Nsukka. They include: Test strips (Pyrex, England), PCV tubes (Pyrex, England), Haematocrit Centrifuge (Vickas Ltd, England), Colorimeter (EI Scientific Co. India), Measuring Cylinder (Pyrex, England), Weighing Balance (Vickas Ltd, England), Centrifuge (Vickas Ltd, England), Spectrophotometer (E312 Model) (Jenway, UK), Timer test tubes (Pyrex, England), Haemoglobin graph (Jenway, UK), Whatman No 1 filter paper, Water bath (Gallenkamp, England), mechanical grinder (Vickas Ltd, England), Muslin cloth, Flat bottomed flask (Pyrex, England). Light microscope (Olympus), chemical balance (Gallenkamp, England), micro – pipettes (Perfect, USA), microscope slides, capillary tube, Refrigerator (Haier thermocool, England), microscope (XSZ – 107BN, India), counting chamber (MC Qiujing, China). 2.1.5 Chemicals and Reagents All the chemicals that were used in this study were of analytical grade, and were products of Sigma Aldrich, USA, British Drug House (BDH) (England), Burgoyne, (India), Harkin and Williams, (England), Qualikems (India), Fluka (Germany), May and Baker (England). The commercial kits used for the assays were products of Randox, USA and Teco (TC), USA. 2.2 Methods 2.2.1 Extraction Procedure The fresh stem-bark of P. curatellifolia was cut into smaller sizes and air-dried to a constant weight for three months. The dried stem bark was then pulverized into powdered form with the aid of a mechanical grinder. Thereafter, the coarsely powdered (700 g) bark was subjected to cold maceration using absolute ethanol (2.5 L) for seventy-two hours in a 5 L transparent plastic container. It was intermittently stirred with the aid of a glass rod. Thereafter, it was filtered with a muslin cloth to separate the marc from the micelle. Further filtration was carried out using Whatman grade 1 qualitative filter papers. The filtrate was concentrated using vacuum evaporation and stored in a sterile screw-capped bottle and stored until needed for analysis. 2.2.2 Determination of the Percentage Yield of the Ethanol Extract of P. curatellifolia Stem-bark The percentage yield of the ethanol extract was determined from the weight of the dried pulverized stem-bark before maceration and the weight of the crude extract after concentration following the formula below; 2.2.5 Acute Toxicity Studies A total of 18 albino mice weighing 14-21 g were used to determine the acute toxicity and the median lethal dose of the extract using the method of Lorke (1983). The study was done in two phases, with both phases having three mice each in three groups. In phase 1, groups 1, 2, and 3 received 10 mg/kg b.w, 100 mg/kg b.w, and 1000 mg/kg b.w respectively. Groups 1, 2, and 3 in phase 2 received 1,600 mg/kg b.w, 2,900 mg/kg b.w and 5,000 mg/kg b.w of the extract respectively. The mice were then observed for 24 hours for lethality or any behavioural changes that shows signs of toxicity. 2.2.6 Parasite Inoculation For inoculum preparation, donor mouse blood infected with P. berghei was used. Blood was drawn from the donor mouse via heart puncture and serially diluted in normal saline to create a suspension containing approximately 1x10 6 infected RBCs per 0.2 ml suspension. This suspension (0.2 ml) was injected intraperitoneally into the experimental animals to initiate infection. Malaria infection was confirmed after 72 hours of inoculation. 2.2.7 Experimental Design The inoculated animals (30 in number) were then randomly divided into six (6) groups of five (5) mice each and treated for four consecutive days (Peters et al ., 1975). On the fifth day, the tail tip of each mice was cut with razor and blood collected for determination of parasitemia. Thereafter, blood samples for hematological and biochemical studies were collected via ocular puncture into EDTA bottles and plain bottles respectively. The animals were subsequently sacrificed and the liver homogenate was used for further biochemical studies. Table 1 : Experimental design Groups Description Treatment Group 1 Normal control Administered with distilled water. Group 2 Positive control Administered with 0.2 ml Plasmodium berghei- infected blood and no treatment Group 3 Standard control Administered with 0.2 ml Plasmodium berghei- infected blood and treated with 20/120 mg/kg b.w. of arthemeter/lumefantrine (standard drug) Group 4 Treatment group 1 Administered with 0.2 ml Plasmodium berghei- infected blood and treated with 200 mg/kg b.w. of P. curatellifolia Group 5 Treatment group 2 Administered with 0.2 ml Plasmodium berghei- infected blood and treated with 400 mg/kg b.w. of P. curatellifolia Group 6 Treatment group 3 Administered with 0.2 ml Plasmodium berghei- infected blood and treated with 600 mg/kg b.w. of P. curatellifolia 2.2.8 Determination of Malaria parasitemia The method described by Dacie and Lewis (2011) was used to determine the malaria parasitemia (Mp+). The tip of the tail of each mouse was cut with a razor and gently squeezed to get a drop of blood unto the Centre of a microscopic slide. The blood was immediately spread on the slide with the aid of smooth-edged slide spreader to make a film of blood. The microscopic slide was then labelled at one end with black lead pencil and air dried in a horizontal position. The air-dried blood films were Placed on a staining rack, flooded with Leishman stain and left for 30 s to 1 min to fix. Thereafter, twice as much buffered distilled water (pH 7.2) from a plastic wash bottle was poured on the films, and then left to stain. After 10 minutes the stain was washed off with water. Finally, the back of the slides was wiped and placed in draining rack and allowed to air dry. After drying, the stained films were placed on counting chamber and viewed under the microscope at 100x magnification. The percentage parasitemia was determined from this. 2.2.9 Statistical Analysis The results were expressed as Mean ± standard deviation and test of statistical significance was carried out using one-way analysis of variance (ANOVA). The statistical package used was the Statistical Product and Service Solutions (SPSS) version 21, the result being considered significant at p < 0.05. Results 3.1 Percentage Yield of Ethanol Extract of Parinari curatellifolia Stem-Bark Seven hundred grams (700 g) of the coarsely powdered Parinari curatellifolia stem bark yielded 57.57 g of extract which is 8.23% extract after extraction with absolute ethanol. 3.2 Qualitative Phytochemical Screening of Ethanol Extract of Parinari curatellifolia Stem Bark The phytochemical screening carried out on the ethanol extract of Parinari curatellifolia stem bark showed it contains the secondary metabolites as shown in Table 3. Table 2: Secondary metabolites found in ethanol extract of Parinari curatellifolia stem bark Phytochemical constituents Presence Tannin +++ Phenolics +++ Saponin ++ Flavonoids ++ Steroids + Glycosides ++ Terpenoids ++ Alkaloid ++ Key: Highly present +++ Moderately present ++ Slightly present + 3.3 Quantitative Phytochemical Composition of Ethanol Extract of Parinari curatellifolia Stem Bark The table below shows the quantitative phytochemical composition of ethanol extract of Parinari curatellifolia stem bark. Table 3. Quantitative phytochemical constituents of ethanol extract of Parinari curatellifolia stem bark Phytochemical constituent Concentration (mg/100g) Tannin 1771.69 Phenols 1281.99 Saponin 123.10 Flavonoids 493.83 Steroid 51.58 Glycosides 645.68 Terpenoids 246.23 Alkaloids 709.59 3.4 Acute Toxicity of Ethanol Extract of Parinari curatellifolia Stem Bark The result of the acute toxicity test of ethanol extract of Parinari curatellifolia stem bark showed there was no mortality recorded as a result of the extract at the doses tested, ranging from 10 mg/kg b.w to 5000 mg/kg b.w as can be seen in Table 4. Table 4. Phases I & II of acute toxicity test of ethanol extract of Parinari curatellifolia stem bark extract Phase/Groups Dosage of extract (mg/kg b.w) Mortality Phase I Group 1 10 0/3 Group 2 100 0/3 Group 3 1000 0/3 Phase II Group 1 1600 0/3 Group 2 2900 0/3 Group 3 5000 0/3 n = 3 3.5 Effect of Ethanol Extract of Parinari curatellifolia Stem Bark on the Percentage parasitemia of Plasmodium berghei -infected Mice Table 5 shows the percentage parasitemia of the mice treated with the ethanol extract of Parinari curatellifolia stem bark. Before the treatment, the percentage parasitemia of the mice that were infected with malaria (groups 2 – 6) were significantly higher (p 0.05) difference among all the infected groups. After the treatment however, the percentage parasitemia in groups 3 to 6, were significantly lower (p 0.05) difference in the percentage parasitemia of the treated groups. Table 5: Percentage parasitemia of Plasmodium berghei -infected mice treated with ethanol extract of Parinari curatellifolia stem bark Group Parasitemia (%) Before treatment After treatment Reduction 1 0.00 ± 0.00 a 0.00 ± 0.00 a 0.00 ± 0.00 2 70.20 ± 10.62 b 103.00 ± 3.32 c - 32.80 ± 10.47 3 69.80 ± 11.30 b 6.40 ± 1.95 b 63.40 ± 11.33 4 76.20 ± 6.87 b 8.40 ± 2.07 b 67.80 ± 7.19 5 74.00 ± 3.67 b 6.40 ± 1.67 b 67.60 ± 3.21 6 78.00 ± 5.66 b 5.40 ± 2.51 b 72.60 ± 6.87 Results are expressed in Mean ± SD (n = 5) Mean values with different letters as superscript down the column are considered significant at P < 0.05. Group 1 : Normal control (Unparasitized and treated with distilled water) Group 2: Positive control (Parasitized and untreated) Group 3: Standard control (Parasitized and treated with 20/120 mg/kg b.w Artemether/lumefantrine) Group 4: Test group (Parasitized and treated with 200 mg/kg b. wt of EEPCSB) Group 5: Test group (Parasitized and treated with 400 mg/kg b.w of EEPCSB) Group 6: Test group (parasitized and treated with 600 mg/kg b.w of EEPCSB) In this study, the extract significantly (p < 0.05) reduced the percentage parasitemia of the treated animals compared to the untreated group. This corroborates the earlier report of the antiplasmodial activity of Parinari curatellifolia stem bark extract (Omoniwa et al ., 2021). This antimalarial effect of this extract could be attributed to the activities of bioactive compounds found in it, most likely the alkaloids, terpenoids and/or saponins. These compounds, acting singly or in synergy could have exerted a cytotoxic effect on the parasite. This result shows that Parinari curatellifolia stem bark is effective in the managing of malarial and can serve as source of a lead compound for a novel antimalarial drug. 3.6 Effect of Ethanol Extract of Parinari curatellifolia Stem Bark on Some Hematological Parameters in Plasmodium berghei -infected Mice Table 6 below shows the effect of the ethanol extract of Parinari curatellifolia stem bark on mice induced with malaria. 3.7 Effect of Ethanol Extract of Parinari curatellifolia Stem Bark on Some Liver Function Parameters of Plasmodium berghei -infected Mice In the Table 7 below, the result of the liver function parameters of malaria infected mice treated with ethanol extract of Parinari curatellifolia stem bark (EEPCSB) is shown. Table 7: Liver function parameters of Plasmodium berghei infected mice treated with ethanol extract of Parinari curatellifolia . Treatment Liver Function Parameters Group AST activity ALT activity ALP activity Bilirubin Conc. (IU/L) (IU/L) (IU/L) (mg/dl) Group 1 19.00 ± 1.22 a 29.80 ± 1.48 a 43.20 ± 2.59 a 4.74 ± 0.70 a Group 2 42.20 ± 1.79 c 66.68 ± 2.41 c 86.00 ± 4.74 d 8.02 ± 0.70 b Group 3 23.20 ± 2.28 b 34.80 ± 3.03 b 49.60 ± 1.52 bc 4.66 ± 0.60 a Group 4 23.60 ± 2.61 b 32.20 ± 1.78 ab 46.20 ± 1.48 ab 4.49 ± 0.19 a Group 5 21.60 ± 1.52 b 32.20 ± 2.74 ab 47.40 ± 4.78 abc 4.60 ± 0.61 a Group 6 23.20 ± 1.92 b 30.40 ± 2.30 a 50.80 ± 1.64 c 4.69 ± 0.61 a Results are expressed in Means ± Standard Deviation (n = 5) Mean values with different letters as superscript down the column are considered significant at p < 0.05. Group 1: Normal control (Unparasitized and treated with distilled water) Group 2: Positive control (Parasitized and untreated) Group 3: Standard control (Parasitized and treated with 20/120 mg/kg b.w Artemether/lumefantrine) Group 4: Test group (Parasitized and treated with 200 mg/kg b. wt of EEPCSB) Group 5: Test group (Parasitized and treated with 400 mg/kg b.w of EEPCSB) Group 6: Test group (parasitized and treated with 600 mg/kg b.w of EEPCSB) 3.8 Effect of Ethanol Extract of Parinari curatellifolia Stem Bark on the Lipid Profile of Plasmodium berghei -infected Mice The lipid profile of the malaria infected mice treated with ethanol extract of Parinari curatellifolia is shown in the Table 8 below. Table 8: Lipid profile of Plasmodium berghei infected mice treated with ethanol extract of Parinari curatellifolia stem bark Treatment Lipid Profile Group Total Cholesterol TAG Conc. HDL Conc. LDL Conc. (mg/dl) (mg/dl) (mg/dl) (mg/dl) Group 1 94.00 ± 3.16 a 104.80 ± 3.03 a 84.00 ± 3.16 e 20.20 ± 2.28 a Group 2 116.20 ± 4.60 d 130.00 ± 6.20 c 33.20 ± 2.28 a 65.00 ± 3.16 c Group 3 105.40 ± 2.41 c 113.60 ± 3.21 b 64.20 ± 1.79 b 25.00 ± 2.24 b Group 4 98.40 ± 4.93a b 111.60 ± 3.29 b 69.20 ± 5.54 bc 26.40 ± 3.05 b Group 5 103.00 ± 4.36 bc 109.00 ± 2.12 ab 73.20 ± 4.76 cd 24.60 ± 2.19 b Group 6 100.00 ± 3.54 b 108.460 ± 2.19 ab 75.60 ± 4.83 d 26.00 ± 4.18 b Results are expressed in Means ± Standard Deviation (n = 5) Mean values with different letters as superscript down the column are considered significant at p < 0.05. Group 1: Normal control (Unparasitized and treated with distilled water) Group 2: Positive control (Parasitized and untreated) Group 3: Standard control (Parasitized and treated with 20/120 mg/kg b.w Artemether/lumefantrine) Group 4: Test group (Parasitized and treated with 200 mg/kg b. wt of EEPCSB) Group 5: Test group (Parasitized and treated with 400 mg/kg b.w of EEPCSB) Group 6: Test group (parasitized and treated with 600 mg/kg b.w of EEPCSB) 3.9 Effect of Ethanol Extract of Parinari curatellifolia Stem Bark on the Malondialdehyde Concentration and Antioxidant Markers in Plasmodium berghei -infected Mice Table 9 shows the antioxidant markers and the malondialdehyde concentration of the Plasmodium berghei -infected mice treated with ethanol extract of Parinari curatellifolia stem bark. Table 9: Malondialdehyde concentration and antioxidant markers of Plasmodium berghei infected mice treated with ethanol extract of Parinari curatellifolia stem bark Treatment MDA Conc. SOD activity Catalase GSH Conc. group (mg/dl) (IU/L) (IU/L) (mg/dl) Group 1 3.79 ± 0.90 a 8.64 ± 0.22 c 0.58 ± 0.32 bc 3.07 ± 0.60 abc Group 2 8.85 ± 0.43 c 3.11 ± 0.73 a 0.23 ± 0.11 a 5.42 ± 0.57 d Group 3 4.97 ± 0.72 b 7.32 ± 1.24 b 0.51 ± 0.07 b 3.66 ± 0.31 c Group 4 4.63 ± 0.02 ab 8.26 ± 0.57 b 0.58 ± 0.03 bc 3.41 ± 0.73 bc Group 5 4.80 ± 0.96 b 8.51 ± 0.85 c 0.60 ± 0.05 c 2.81 ± 0.67 ab Group 6 4.79 ± 0.55 b 8.74 ± 0.44 c 0.56 ± 0.04 bc 2.35 ± 0.21 a Results are expressed in Means ± Standard Deviation (n = 5) Mean values with different letters as superscript down the column are considered significant at p < 0.05. Group 1: Normal control (Unparasitized and treated with distilled water) Group 2: Positive control (Parasitized and untreated) Group 3: Standard control (Parasitized and treated with 20/120 mg/kg b.w Artemether/lumefantrine) Group 4: Test group (Parasitized and treated with 200 mg/kg b. wt of EEPCSB) Group 5: Test group (Parasitized and treated with 400 mg/kg b.w of EEPCSB) Group 6: Test group (parasitized and treated with 600 mg/kg b.w of EEPCSB) Discussion The phytochemical screening of the ethanol extract of Parinari curatellifolia showed the presence of saponins, phenolics, flavonoids, alkaloids, glycosides, tannins, terpenoids and steroids. The quantity of steroids detected was negligible. Alkaloids, saponins, flavonoids, terpenoids, and glycosides were detected in moderate to high amounts. Our result was in agreement with that of (Halilu et al. , 2008) which reported detection of tannins, saponins, flavonoids, steroids, glycosides in methanolic extract of Parinari curatellifolia stem bark, but showed no detection of alkaloids. Furthermore, our findings is also comparable to those of (Sylvanus et al. , 2014), differing only in the absence of flavonoids in ethanolic stem bark extract of Parinari curatellifolia, of their report. The report of Issakou et al (2022) also showed the detection tannins, saponins, alkaloids, flavnoids, terpenoids, and steroids which is in tandem with our result. In addition to phenolics, tannins, saponins, glycosides, alkaloids, terpenoids, flavonoids and steroids, (Omoniwa et al ., 2021) reported the detection of resins in ethanolic extract of P.curatellifolia stem bark, which was not included in our study. The plant extract's wide range of secondary metabolites may explain why it is used in ethnomedicine to treat a number of illnesses in Africa, such as malaria, fever, toothaches, fractures, pneumonia, cancer, inflammation, bacterial infections, and diabetes ( Halilu et al ., 2008; Kuntze et al ., 2017; Mawire et al ., 2021). Alkaloids are class of small nitrogen- containing organic molecules found in plants. Many alkaloids are great therapeutic agents which are used to treat various diseases including malaria, diabetes, cardiac dysfunction e.tc (Ain et al ., 2016). Quinine, an alkaloid isolated from the bark of cinchona tree is an antimalarial drug. Isoquinoleine alkaloid extract from Fumaria capreolata , exerted antioxidant, analgesic, and intestinal anti-inflammatory activities in DNBS model of experimental colitis in mice. Antitumor efficacy is demonstrated by the alkaloids zephyrantine and narcyclasine-glucoside (Noureddine Bribi, 2018). Vincristine, an alkaloid from Vinca rosea, is an anticancer medicine, sanguinaria from Sanguinaria canadensis is antibacterial, and ementine from Carapichea ipecacuanha is antiprotozoal (Roy, 2017; Heinrich et al., 2021). Flavonoids have been reported to possess antioxidant effect, anti-bacterial effect, anti-cancer effect cardioprotective effect, anti-inflammatory effect, and immune system promoting effect (Tungmunnithum et al ., 2018). Phenolics and flavonoids are the largest secondary metabolites from plants with antioxidant properties. Chrysosplenetin, a known polymethoxylated flavonoids in Artemisia annua L . and other Chinese herbal plants, was reported to boost the antimalarial activity of Artemisinin when co-administered (Patel, 2022). Many flavonoids including triglycoside of kaempferide, kaempferol-O-rutinoside, kaempferol-3-O-b-D-glucopyranosyl isoorientin, isovitexin, vitexin, 5,6-dimethoxyflavone, 2-hydroxyisoflavanone, naringenin, naringenin 7-O-b-D-glucoside, 5-hydroxy-7,8-dimethoxyflavanone 5-rhamnoside, kaempferol 3-rhamnoside-7-xylosyl-(1-2)-rhamnoside have been found to possess antimicrobial activities (Mierziak et al ., 2014). Plant tannins are polyphenols that are generically classed as hydrolyzed and condensed tannins. They have been reported to possess antioxidant, anti-bacterial, anti-inflammatory, antiparasitic and antiviral activities (Tong et al ., 2022). Saponins are naturally occurring surface-active glycosides with a particular foaming property that are present in both wild and cultivated plants as steroidal and triterpenoid saponins, respectively. They are reported to possess a variety of biological activities including membrane-permeabiliting, immunostimulant, hypocholesterolaemic and anticarcinogenic, antiparasitic, antiprotozoal, cytotoxic, antimicrobial and antioxidant (Guclu-Ustundag and Mazza, 2007; Desai et al ., 2009). The acute toxicity study of ethanol extract of Parinari curatellifolia stem bark indicated that the median lethal dose (LD 50 ) is above 5000 mg/kg b.w, as there was no mortality recorded due to the extract in mice up to 72 hours in all the doses tested. This indicates that the extract is safe for comsumption for both animals and humans. Ogbonnia et al 2008 had earlier reported the estimated LD 50 of ethanol extract of Parinari curatellifolia seeds to be 7.2 g/kg b.w,which supports our result. However, (Atawodi et al ., 2103) reported that the LD 50 of methanolic root extract of P. curatellifolia was 1,265 mg/kg b.w in rats. It could therefore be inferred that stem bark is safer compared to the root extract. The percentage parasitemia of the animals treated with the extract in this study was significantly (p < 0.05) reduced compared to the untreated group. This result is in tandem with that of (Omoniwa et al. , 2021) which had earlier reported the in vitro antiplasmodial activity of ethanol extract of Parinari curatellifolia stem bark. The observed antimalarial effect of this extract is attributable to the activities of the constituent bioactive compounds including alkaloids, terpenoids, and/or saponins. These compounds could have acted singly or in synergy to exert cytotoxic effect on the malarial parasite. Further studies of the compounds in the extract could lead to isolation of lead compounds for novel antimalarial drug. The result of this study indicated that the hematological parameters including packed cell volume (PCV), red blood cell (RBC) count, and haemoglobin (Hgb) concentration of the treated animals were significantly (p < 0.05) increased compared to the untreated animals, while the white blood cell (WBC) count of the treated animals was significantly (p < 0.05) lower compared to the untreated group. The observed decrease in the PCV, RBC count and Hgb concentration of the untreated infected animals was in accord with previous studies (Francis et al., 2014; Nefsu and Amsalu, 2019), which adduced that the phagocytosis of parasite-infected RBC, decreased RBC formation in the bone marrow, and mechanical destruction of the parasitized red blood cells accounted for the decline. In addition, the observed rise in the WBC count of the untreated infected mice was consistent with past studies (Francis et al ., 2014; Kotepui et al ., 2015; Sirak et al ., 2016), which all suggested that such increase was attributable to enhanced leucocyte release in response to plasmodium infection. Hemolysis and anaemia are known features of malaria, due to the invasion of the red blood cells and digestion of heamoglobin by plasmodium parasite (Kavishe et al., 2017; Mawson, 2013). This accounts for the reduced PCV, RBC count and Hgb concentration in malarial infected subjects as observed in the untreated infected animals. However, there was an increase in the PCV, RBC count, Hgb concentration and a decrease in WBC count of the animals when treated with the extract, indicating a reversal of the hematological aberrations caused by the Plasmodium parasites. This result suggests that the extract possesses antiplasmodial activity, leading to drastic reduction in parasitemia and hemolysis in the treated animals. The erythrocytic schizonts attack the red blood cells, damaging the membrane through the byproducts of its haemoglobin metabolism such as ferriprotoporphyrin 9, which exert oxidative stress and lipid peroxidation to the red blood cells, causing hemolytic anemia in malaria disease. Our result also agrees with the report of (Ajibola et al ., 2012) which indicated that non-malaria infected persons has higher PCV and Hgb concentration than malaria patients. Sirak et al . (2016) had also reported that density of parasitemia determined the changes in hematological parameters in malaria patients. Patients with high malaria parasite density had lower PCV and Hgb concentration with higher WBC count while patients with low parasitemia had higher PCV and Hgb concentration with lower WBC count. Anemia is determined by estimating the packed cell volume. A PCV value of less than 30% indicates anemia (Osonuga et al ., 2006). The reduced PCV indicated anemia in the infected animals. Anemia is a common and frequently severe complication of malaria, particularly in young children and pregnant women, and accounts for more than 50% of malaria-associated mortality in endemic areas (Mohandas and An, 2013). Malaria-induced anemia is caused by multiple factors including lysis of parasitized erythrocytes, immune-mediated hemolysis, dyserythropoietic changes and shortened life span of erythrocytes (Osonuga et al ., 2006) The ethanol extract of Parinari curatellifolia was able to restore the PCV of the treated animals in groups 5 and 6 to a level that is statistically non-significantly different compared to the normal control. This also demonstrates that the extract was able to stop hemolysis of red blood cells and may have the ability to increase erythrocyte synthesis in mice. This finding also indicates that the extract reversed anemia in mice to a comparably the same level as artemether/lumefantrine. The clinical symptoms and pathologies associated with malaria are as a result of cellular and membrane changes in the human red blood cells (RBC) induced by the intra-erythrocytic parasite. These structural and biochemical changes, mediated by interactions between red cell membrane and parasite-expressed proteins, impart rigidity and adhesiveness to the infected red blood cells (iRBC) making them adhesive for a variety of cell types, including vascular endothelial cells (cytoadherence), platelets, and other infected or uninfected red cells. Infected red cells are eventually ruptured by the matured intra-erythrocytic parasite for another cycle of red cell invasion (Mohandas and An, 2013). Rigid RBCs contribute to disease pathology through sequestration of these cells and obstruction of the microvasculature, however its impact is primarily for the clearance of the parasitized cells in the spleen (Deng et al ., 2015). The extract could have induced reduced cell deformability of the infected red cells thereby enhancing their host-mediated clearance in the inter-endothelial cleft of the spleen. This idea is supported by the fact that reduced deformability of infected red blood cell was reported to be a common feature antimalarial drug. Artemether, the standard drug in this study, is thought to act by a mechanism which generate reactive oxygen species that contribute to reduced iRBC deformability through lipid peroxidation (Deng et al ., 2015). The malaria parasite digest haemoglobin, the iron containing protein of the red blood cells, during its 48-hour intra-erythrocytic cycle to obtain amino acids, leading to depletion of haemoglobin and accumulation of free heme (Joof et al ., 2021). The result of this study showed that haemoglobin concentration in the untreated animals significantly (p < 0.05) reduced compared to the uninfected animals. The administration of the extract, on the other hand, significantly (p < 0.05) raised the haemoglobin concentration in the test groups. This result is consistent with prevous studies (Aragaw et al. , 2020; Joshua et al ., 2020; Enechi et al ., 2021). The activity of some of the secondary metabolites in the extract could be responsible for the observed higher haemoglobin concentration in the treated animals. It could be adduced that some of the metabolites inhibited the digestion of haemoglobin by the parasite. This is as some quinoline drugs such as chloroquine and amodiaquine have been found to inhibit haemoglobin degradation (Famin and Ginsgurg, 2002). The increased white blood cell count found in the untreated animals indicates the immune response of the animals to the plasmodium infection. Reports have shown that severe malaria is characterized by leukocytosis. The extract however reduced the WBC count in the treated animals. This confirms that the extract was able to clear the infecting Plasmodium parasite thereby reducing the stimulation of leukocytosis. The overall health of the liver is determined by a number of blood tests generally known as liver function tests. The tests evaluate the levels of several enzymes, proteins, and other chemicals produced by the liver. This test is employed in the assessment of liver damage, determine side effect of certain drugs, diagnosis of liver disease and monitoring of its treament. Alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), gammaglutamyl transferase, serum bilirubin, prothrombin time, and serum albumin are some of the commonly available liver function test. Several liver activities such as anion excretion (bilirubin), hepatocellular integrity (transaminases), bile production and subsequent free flow (bilirubin and ALP), and protein synthesis (albumin) are reflected by this test (Hoekstra et al ., 2013; van Beek et al ., 2014). In this study, the activities of serum liver enzymes namely AST, ALT and ALP were assayed, while the concentration of the serum bilirubin was determined to assess hepatocellular damage and impairment of liver function due to malaria parasite, as well as the effect of the ethanol extract of Parinari curatellifolia stem bark on the liver. The result indicated that there was significant lowering of in the activities of AST, ALT and ALP as well as the concentration of total bilirubin in all the test groups when compared to the untreated group. The untreated group demonstrated an increase in all of the assessed metrics when compared to the normal control group. The increase in the activities of aminotransferases (AST, ALT) and alkaline phosphatase may be as a result of possible hepatocellular damage (Gowda et al ., 2009) while the increased hyperbilirubinemia could be due to parenchymal liver cell destruction and biliary blockage caused by malaria parasite, liver schizonts (Limdi and Hyde, 2003; Hoekstra et al ., 2013). This finding is in agreement with earlier research (Enechi et al ., 2021; Iyiola, 2020; Joshua et al ., 2020) which discovered higher hepatic enzyme activity and hyperbilirubinemia in mice infected with Plasmodium berghei . The reduction in increased AST, ALT, and ALP levels demonstrated that the extract was efficient in correcting plasmodium parasite-induced hepatocellular damage. It is evident from this result that the has hepatoprotective ability which could be due to the synergistic action of the several secondary metabolites in the extract. The hepatoprotective effect could also be due to the action of a single phytochemical in the extract. The concentration of bilirubin in plasma provides indirect information on the liver's uptake, conjugation, and excretion functions. Elevated plasma concentration of bilirubin are specific markers for serious liver injury and, consequently, liver function loss (Hoekstra et al ., 2013). The reduction in plasma concentration of bilirubin of mice infected with Plasmodium berghei and treated with the extract shows that the extract reversed liver cell damage and restored liver cell function. This is congruent with the findings of Joshua et al . (2020) which demonstrated that in mice with Plasmodium berghei infection, E. sengalensis extract reduced the total bilirubin levels. Visser et al ., 2017 had reported changes in lipid profile associated with malaria infection. This study determined the total cholesterol, HDL-cholesterol, LDL-cholesterol, and triglycerides. The result of the showed that the ethanol extract of Parinari curatellifolia stem bark significantly (p < 0.05) reduced the total cholesterol, triglyceride and LDL cholesterol while significantly (p < 0.05) raising the HDL cholesterol in the treated animals compared to the untreated ones (positive control). Previous studies (Enechi et al ., 2021; Joshua et al ., 2020), supports this result. This study further revealed that malaria infection results in mixed hyperlipoproteinemia in mice, which is characterized by increased total cholesterol, triglyceride, LDL-cholesterol and decreased HDL-cholesterol (Natesan and Kim, 2021). However, the mechanism involved in the changes in plasma lipid profile related to malaria is yet to be completely understood. It has been suggested to be partly host-related (Acharya and Patil, 2009). This is in contrast with the reportedly observed dyslipidemia in human malaria which showed increased triacylglycerol and decreased HDL, LDL and total cholesterol plasma concentrations in infected patients ( Visser et al ., 2013; Dias et al ., 2016; Sirak et al ., 2016; Visser et al ., 2017). This observed difference could be due to differing pattern of lipid metabolism in mice and humans. The accumulation of triglyceride and LDL-cholesterol causes damage to the arteries, contributing to cardiovascular disease, while on the other hand HDL-cholesterol is useful in helping the body to get rid of bad cholesterol (Natesan and Kim, 2021). The finding of this study reveal that the extract possesses anti-lipidemia activity as it was able to reduce total cholesterol, triglyceride and LDL-cholesterol in the treated mice. The anti-lipidemia effect of this extract as found in this study also supports the previous report of Ogbonnia et al . (2008), which demonstrated that the hydro ethanolic extract of P. curatellifolia seed significantly reduced plasma lipid concentration in diabetic rats. Malondialdehyde (MDA) is used as an indirect measure of the cumulative lipid peroxidation as it is a unstable end product of lipid peroxidation (Mao et al ., 2019). This aldehyde known for its high cytotoxicity and carcinogenicity is a product of free radicals’ attack on phospholipids. Increased level of MDA is associated with decreased antioxidant parameters (Maurya et al ., 2021), and this is as a result of increased production of oxidative species (Percário et al ., 2012), associated with malaria infection. The result of this study indicated that MDA concentration significantly (p < 0.05) reduced in the treated mice compared to the positive control (the untreated infected mice). This shows that the extract has free radical scavenging activity which could be attributed to the phytochemical constituents in the extract including flavonoids, phenols, tannins, and terpenoids. This finding is consistent with previous studies which showed Parinari curatellifolia to be a potent scavenger of nitrite free radicals (Boora et al ., 2014) and inhibitor of phospholipid peroxidation due to free radical activity (Ndhlala et al ., 2006) . Superoxide dismutase (SOD) is utilized both the human host and Plasmodium parasite as first line of defense against superoxide radicals. They metalloproteins that dismutate superoxide radicals (O 2 - ) into hydrogen peroxide (H 2 O 2 ) and oxygen (O 2 ) using copper/zinc (Cu/Zn), iron (Fe), or manganese (Mn) as cofactors (Kavishe et al ., 2017). The SOD activity of the mice treated with the extract of Parinari curatellifolia stem bark was found to significantly (p < 0.05) increase compared to the untreated animals. The SOD activity of the untreated group decreased significantly compared to the uninfected group. This could be due to increased concentration of oxidants generated by the host cell in response to malaria infection, leading to the depletion of the enzyme and reduced activity observed. The extract however restored the activity of the enzyme to a level that is non-significantly different from the normal control in all the treated groups. This effect could be attributed to the antioxidant activity of the extract. The extract contains antioxidative phytochemicals such as flavonoids, phenols and tannins. Previous studies in rats (Olaleye et al ., 2014; Yakubu, 2012; Atawodi et al ., 2013), has showed that Parinari curatellifolia possess antioxidant activity, is consistent with the result of our study. The catalase activity of the treated animals in this study was also found to increase significantly (p < 0.05) in the group of mice treated with the extract compared to the untreated group. The catalase activity in the untreated group significantly decreased compared to the normal control, possibly due to excessive generation of hydrogen peroxide (H 2 O 2 ). Catalase limits the accumulation of H 2 O 2 (Kavishe et al ., 2017) . Previous studies has shown that malaria infection is associated with decreased activity of catalase (Vasquez et al ., 2021). The result of this study antioxidative activity of the ethanol extract of Parinari curatellifolia stem bark. However, the result showed that the reduced glutathione (GSH) concentration significantly increased in the untreated group compared to the normal control (unparasitized mice). This observation is consistent with that of Stocker et al ., 1985, which reported increased GSH concentration in malaria patient with high number of infected red blood cells due to the enhancement of glutathione reductase. They suggested that significant portion of the increase was associated with the production of GSH by the parasite itself. The administration of the extract resulted in significant (p < 0.05) decrease in GSH concentration of the treated group compared to the untreated group. The effect might be due to generation of oxidative stress by a phytochemical component of the extract, as it is known that some anti-malarial drugs exert their antiplasmodial effect by increasing oxidative stress on the parasite. A study by (Akanbi et al ., 2010) had reported lower levels of GSH in malarial patients treated with anti-malarial drug compared to patients who had not taken drug. Conclusion The findings from this study so far have shown that the ethanol extract of Parinari curatellifolia stem bark can serve as an effective and safe regimen for the treatment of malaria in the mouse model. The extract is composed of phytochemicals that can be beneficial to human health, and caused no mortality in the acute toxicity test in mice, showing its safety for human use. The extract also effectively reduced parasitemia in infected mice, while restoring hematological aberrations including PCV, RBC count, WBC count, haemoglobin concentration and biochemical aberrations including liver enzymes activities, bilirubin concentration and lipid profile, induced by the Plasmodium parasite. This study also showed that the extract countered lipid peroxidation by reducing the MDA level in treated animals as well as restored the antioxidant markers to the levels found in the uninfected animals. This study has so far demonstrated that ethanol extract of Parinari curatellifolia stem bark is effective and safe for the treatment of malaria, thereby justifying its use for managing malaria in various part of the African continent. This plant can as well serve as a source of lead compound for the development of a novel, safe and effective antimalarial drug if exploited. Abbreviations ACT Artemisinin combination therapy ALT Alaninine aminotranferase ALP Alkaline phosphatase AST Aspartate aminotransferase CAT Catalase DCM-MeOH Dichloromethame-methanol EEPCSB Ethanol extract of Parinari curatellifolia stem bark FFA Free fatty acids FP Ferriprotoporphyrin GGT Gamma-glutamyl transferase GGTP Gamma glutamyl transpeptidase G6PD Glucose-6-phosphate dehydrogenase GSH Reduced glutathione HDL High density lipoprotein Hgb Haemoglobin IC50 Median inhibitory concentration LDL Low density lipoprotein MCV Mean Cell Volume MDA Malondialdehyde PCV Packed cell volume RBC Red blood cell RNS Reactive nitrogen species ROS Reactive oxygen species SPSS Statistical Product and Service Solution SOD Superoxide dismutase VLDL Very low density lipoprotein WBC White blood cell WHO World Health Organization Declarations Ethics approval This study was conducted in line with the ethical rules and recommendations of the University of Nigeria Ethical committee on the care and use of laboratory animals. Consent for publication Not Applicable Availability of data and materials The datasets used and analyzed during the current study are available from the corresponding author on reasonable request. Competing interests The authors declare that they have no competing interest. Funding This research received no funding from any institution, organization or agency. Authors’ contributions LOM, PEJ, CCA; Conceptualization, Methodology, Software, LOM: data curation, writing – original manuscript preparation, PEJ: supervision, PEJ, CCA, LOM: investigation, validation PEJ, CCA, IO, JOF: writing – reviewing and editing. All authors read and approve the final manuscript. Acknowledgements Not applicable. References Acharya M, Patil SL. Variation in common lipid parameters in malaria infected patients. Indian J. Physiol. Pharmacol. 2009;53(3):271–4. Ain QU, Khan H, Mubarak MS, Pervaiz A. Plant alkaloids as antiplatelet agent: Drugs of the future in the light of recent developments. Front. 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A review on the alkaloids an important therapeutic compound from plants Remediation of environmental contamination View project Micropropagation of Centella asiatica View project. International Journal of Plant Biotechnology. 2017; 3 (2):1–9. Saxena S, Pant N, Jain DC, Bhakuni RS. Antimalarial agents from plant sources. 2003; 85 (9):1314–1329. Sirak S, Fola AA., Worku L, Biadgo B. Malaria parasitemia and its association with lipid and hematological parameters among malaria- infected patients attending at Metema Hospital , Northwest Ethiopia. Pathology and Laboratory Medicine International. 2016; 8 : 43–50. Sylvanus U, Olakunle F, Amos J, Olutayo O. Antibacterial activity and phytochemical evaluation of the leaf root and stem bark extracts of parinari curatellifolia (planch. ex benth). Int J Adv Chem. 2014;2:178-81. Tajbakhsh E, Kwenti TE, Kheyri P, Nezaratizade S, Lindsay DS, Khamesipour F. 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Inter J Basic Appl Chem Sci. 2012;2(3):82-9. Additional Declarations The authors declare no competing interests. Cite Share Download PDF Status: Posted Version 2 posted You are reading this latest preprint version Show more versions Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3586444","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":248734105,"identity":"2391e508-d32a-4834-9056-d7827a0eb8d6","order_by":0,"name":"Lomi Oruchukwu Michael","email":"data:image/png;base64,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","orcid":"","institution":"University of Nigeria, Nsukka Enugu State","correspondingAuthor":true,"prefix":"","firstName":"Lomi","middleName":"Oruchukwu","lastName":"Michael","suffix":""},{"id":248734106,"identity":"6e0d6aeb-a132-40dc-9530-8164ee0c6d45","order_by":1,"name":"Parker Elijah Joshua","email":"","orcid":"","institution":"University of Nigeria, Nsukka Enugu State","correspondingAuthor":false,"prefix":"","firstName":"Parker","middleName":"Elijah","lastName":"Joshua","suffix":""},{"id":248734107,"identity":"8ce38b5e-5215-4772-b6ed-101385634e46","order_by":2,"name":"Christian Chijioke Amah","email":"","orcid":"","institution":"University of Nigeria, Nsukka Enugu State","correspondingAuthor":false,"prefix":"","firstName":"Christian","middleName":"Chijioke","lastName":"Amah","suffix":""},{"id":248734108,"identity":"29f27a3e-eede-48c8-8e9e-652d9c1fcf91","order_by":3,"name":"Ikechukwu Jacob Okoro","email":"","orcid":"","institution":"David Umahi Federal University of Health Sciences","correspondingAuthor":false,"prefix":"","firstName":"Ikechukwu","middleName":"Jacob","lastName":"Okoro","suffix":""},{"id":248734109,"identity":"ccbdb35b-e3c8-4937-9cbb-db471c62234f","order_by":4,"name":"Joshua Ogah Felix","email":"","orcid":"","institution":"University of Nigeria, Nsukka Enugu State","correspondingAuthor":false,"prefix":"","firstName":"Joshua","middleName":"Ogah","lastName":"Felix","suffix":""}],"badges":[],"createdAt":"2023-11-09 17:59:14","currentVersionCode":2,"declarations":{"humanSubjects":false,"vertebrateSubjects":false,"conflictsOfInterestStatement":false,"humanSubjectEthicalGuidelines":false,"humanSubjectConsent":false,"humanSubjectClinicalTrial":false,"humanSubjectCaseReport":false,"vertebrateSubjectEthicalGuidelines":false},"doi":"10.21203/rs.3.rs-3586444/v2","doiUrl":"https://doi.org/10.21203/rs.3.rs-3586444/v2","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":54792244,"identity":"0e46195d-5070-47d2-b2e1-dbe5fb1cd613","added_by":"auto","created_at":"2024-04-16 21:54:46","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":803927,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-3586444/v2/ed1fdb69-9f93-4354-94c4-a0af850cb9e0.pdf"}],"financialInterests":"The authors declare no competing interests.","formattedTitle":"\u003cp\u003e\u003cstrong\u003eAntimalarial effect of ethanol extract of \u003c/strong\u003e\u003cem\u003e\u003cstrong\u003eParinari curatellifolia\u003c/strong\u003e\u003c/em\u003e\u003cstrong\u003e stem-bark in \u003c/strong\u003e\u003cem\u003e\u003cstrong\u003ePlasmodium berghei\u003c/strong\u003e\u003c/em\u003e\u003cstrong\u003e-NK 65 infected mice.\u003c/strong\u003e\u003c/p\u003e","fulltext":[{"header":"Introduction","content":"\u003cp\u003eMalaria has continued to be a major public health problem despite efforts at controlling it. It is widespread throughout tropical and subtropical regions of the world, and Africa carries a disproportionately high share of the global malaria burden, both in terms of total malaria morbidity and mortality. World Health Organization (WHO) reported that, in 2021, 247 million cases of malaria and 619,000 deaths in 87 malaria endemic countries globally were recorded. Africa accounted for 95% of the total cases and deaths. Ninety-six percent (96%) of the cases and deaths took place in 29 of the 87 malaria endemic countries\u0026nbsp;(WHO, 2022). According to the 2022 World Malaria Report, Nigeria had the highest number of global malaria cases (27 % of global malaria cases) in 2021 and accounted for the highest number of deaths (31 % of global malaria deaths).\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eMalaria is caused by\u0026nbsp;\u003cem\u003ePlasmodium\u003c/em\u003e parasites that are transmitted to humans through the bites of infected female \u003cem\u003eAnopheles\u003c/em\u003e mosquitoes. \u003cem\u003ePlasmodium falciparum, Plasmodium malariae, Plasmodium ovale curtisi, Plasmodium ovale wallicker, Plasmodium vivax,\u003c/em\u003e and \u003cem\u003ePlasmodium knowlesi\u003c/em\u003e, are the five species of the parasite that infects humans, with \u003cem\u003ePlasmodium falciparum\u003c/em\u003e being the deadliest\u0026nbsp;(Milner, 2018; Prabhu \u003cem\u003eet al\u003c/em\u003e., 2021). \u003cem\u003ePlasmodium\u003c/em\u003e has a complex life cycle involving both sexual and asexual replications in both the insect and human hosts respectively\u0026nbsp;(Garrido-Cardenas \u003cem\u003eet al\u003c/em\u003e., 2019). During a blood meal, the female Anopheles mosquito transmits the sporozoites to humans. The sporozoites travels through the blood capillaries to the liver where it replicates asexually in the hepatocyte and mature into the liver schizont containing many merozoites, which are released into the blood stream. The merozoites invade the red blood cells and undergo intra-erythrocytic asexual replications and mature into the erythrocytic schizonts. The cycle continues, resulting in acute and chronic parasitemia. Some parasites commit to producing gametocytes, which are picked up by the mosquito during a blood meal. The sporozoites which are transmitted to another person upon blood meal, are produced through the sexual fusion of the gametocytes\u0026nbsp;(Venugopal \u003cem\u003eet al\u003c/em\u003e., 2020). The classic malaria paroxysms, which are marked by recurrent fevers and chills, are caused by the release of merozoites from infected red blood cells (iRBC) (Oliver \u003cem\u003eet al\u003c/em\u003e., 2014).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eDepending on the plasmodium species, the host\u0026apos;s inherent and acquired immunity, and the selection of an appropriate and timely course of therapy, the clinical signs of malaria might differ significantly. Consequently, asymptomatic, uncomplicated and severe are three types of malaria seen in humans\u0026nbsp;(Garrido-Cardenas \u003cem\u003eet al\u003c/em\u003e., 2019). Malaria initially manifests as flu-like symptoms, including fever, chills, headaches, joint pains, nausea, vomiting, coughing, chest discomfort, myalgia, and weakness. Few patients go on to develop severe malaria which leads to coma, acute renal failure, severe anemia, acidosis, pulmonary edema and hypoglycemia (Su \u003cem\u003eet al\u003c/em\u003e., 2019).\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eQuinine has been the foremost drug for treating severe malaria for more than 200 years. It was isolated from the bark of Cinchona species in 1820. Despite the significant limitations of \u0026nbsp;quinine, it has nonetheless had long history of efficacy. The case-fatality rate in severe malaria, when treated promptly with quinine exceeds 20%\u0026nbsp;(Li \u0026amp; Weina, 2010). Chloroquine, which is another important antimalarial drug, was synthesized in 1940. It remained the first line of treatment for uncomplicated malaria, until it was recently replaced with artemisinin-based therapy due to widespread resistance to chloroquine especially by \u003cem\u003ePlasmodium falciparum\u003c/em\u003e (Saxena \u003cem\u003eet al\u003c/em\u003e., 2003). Based on the recommendations of WHO, artemisinin combination therapy (ACT) was introduced in twenty African countries between 2001 and 2004, for treatment of malaria. \u0026nbsp;Currently, ACT is the first-line treatment for uncomplicated \u003cem\u003efalciparum\u003c/em\u003e malaria in Africa, Asia and South America\u0026nbsp;(Maiga \u003cem\u003eet al\u003c/em\u003e., 2021). Nevertheless, as was initially noted in Cambodia in 2006 and expanding to other regions of South-east Asia and Africa, resistance to artemisinin is emerging\u0026nbsp;(Maiga \u003cem\u003eet al\u003c/em\u003e., 2021;\u0026nbsp;Tajbakhsh \u003cem\u003eet al\u003c/em\u003e., 2021), has necessitated the search for a novel antimalarial drug.\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eIn several regions of the world, particularly in malaria-endemic countries like Africa, numerous plants can be used to cure malaria. A good number of these plants have been documented in various ethnobotanical surveys of plants used in treating malaria\u0026nbsp;(Iyamah and Idu, 2015; Ungogo \u003cem\u003eet al\u003c/em\u003e., 2020; Enebeli-Ekwutoziam \u003cem\u003eet al\u003c/em\u003e., 2021). As there has been an increasing resistance to the front-line antimalarial drugs, the quest to discover and develop new anti-malarial drugs has led to various \u003cem\u003ein vitro\u003c/em\u003e and \u003cem\u003ein vivo\u003c/em\u003e studies of plants species used ethno-medically in treating malaria in Africa. A considerable number of plant species have shown appreciable anti-plasmodial and antimalarial activity, however, not much success has been recorded in developing a new antimalarial drug, as only one clinical trial with \u003cem\u003eCochlospermum planchonii\u003c/em\u003e has been carried out till date\u0026nbsp;(Tajbakhsh \u003cem\u003eet al\u003c/em\u003e., 2021).\u003c/p\u003e\n\u003cp\u003e\u003cem\u003e\u0026nbsp;\u003c/em\u003e\u003c/p\u003e\n\u003cp\u003e\u003cem\u003eParinari curatellifolia\u003c/em\u003e Planch ex Benth (Chrysobalanaceae), commonly known as Mbola or Mobola plum, is a large ever green, spreading tree up to 22 metres tall with a single bare stem and a dense, roundish to mushroom shaped crown. The plant\u0026apos;s therapeutic qualities make it extremely valuable. In ethnomedicine, it is used to cure diabetes, fractures, wounds, cuts, and sores as well as fevers, pneumonia, and toothaches\u0026nbsp;(Halilu \u003cem\u003eet al\u003c/em\u003e., 2008; Ogbonnia \u003cem\u003eet al\u003c/em\u003e., 2009). Various \u003cem\u003ein vitro\u003c/em\u003e anti-malarial studies of root, root bark, and stem bark extracts of the plant using various solvents have been carried out with reports of good activity\u0026nbsp;(Omoniwa \u003cem\u003eet al\u003c/em\u003e., 2021; Tajbakhsh \u003cem\u003eet al\u003c/em\u003e., 2021). However, there is no reported \u003cem\u003ein vivo\u0026nbsp;\u003c/em\u003estudy on the anti-plasmodial/antimalarial activity of this plant till date.\u003c/p\u003e"},{"header":"Materials and methods","content":"\u003cp\u003e\u003cstrong\u003e2.1 Materials\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.1.1\u0026nbsp; \u0026nbsp;\u0026nbsp;Plant Materials\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eFresh stem-bark of \u003cem\u003eParinari curatellifolia\u0026nbsp;\u003c/em\u003ewas collected from Agu-orba in Udenu Local Government Area of Enugu State, Nigeria. The stem-bark was identified and authenticated by a taxonomist of the International Centre for Ethnomedicine and Drug Development, Nsukka \u0026nbsp;Enugu State, Nigeria. It was assigned the voucher number InterCEDD/16291 and deposited in the Centre\u0026rsquo;s herbarium.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.1.2\u0026nbsp; \u0026nbsp;\u0026nbsp;Animals\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eForty-eight (48) Albino mice of average body weight of 20 \u0026plusmn; 5g mice were used for this study. The animals were purchased from the Animal House of the Faculty of Veterinary Medicine, University of Nigeria, Nsukka. The animals were kept under standard laboratory condition for one week prior to the commencement of the experiment, with a 12-hour light and dark cycle and maintained on a regular feed (commercial chicken grower\u0026rsquo;s mash) and water \u003cem\u003ead libitum\u003c/em\u003e. They were given adequate care throughout the period of the experiment in accordance with the ethical rules and recommendations of the University of Nigeria Ethical committee on the care and use of laboratory animals.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.1.3\u0026nbsp; \u0026nbsp;\u0026nbsp;Parasite\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eChloroquine-sensitive \u003cem\u003ePlasmodium berghei\u003c/em\u003e NK 65 strain which was used for the study was obtained from blood samples collected from infected donor mice from the faculty of Veterinary Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria. The blood sample was diluted with normal saline before inoculation into the animals.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.1.4\u0026nbsp; \u0026nbsp;\u0026nbsp;Equipment and Instruments\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe equipment used for this study was obtained from the Department of Biochemistry, the Department of Veterinary Medicine of the University of Nigeria Nsukka, and other scientific shops in Nsukka. They include: Test strips (Pyrex, England), PCV tubes (Pyrex, England), Haematocrit Centrifuge (Vickas Ltd, England), Colorimeter (EI Scientific Co. India), Measuring Cylinder (Pyrex, England), Weighing Balance (Vickas Ltd, England), Centrifuge (Vickas Ltd, England), Spectrophotometer (E312 Model) (Jenway, UK), Timer test tubes (Pyrex, England), Haemoglobin graph (Jenway, UK), Whatman No 1 filter paper, Water bath (Gallenkamp, England), mechanical grinder (Vickas Ltd, England), Muslin cloth, Flat bottomed flask\u0026nbsp;(Pyrex, England). Light microscope (Olympus), chemical balance (Gallenkamp, England), micro \u0026ndash; pipettes (Perfect, USA), microscope slides, capillary tube,\u0026nbsp;Refrigerator (Haier thermocool, England), microscope (XSZ \u0026ndash; 107BN, India), counting chamber (MC Qiujing, China).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.1.5\u0026nbsp; \u0026nbsp;\u0026nbsp;Chemicals and Reagents\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAll the chemicals that were used in this study were of analytical grade, and were products of Sigma Aldrich, USA, British Drug House (BDH) (England), Burgoyne, (India), Harkin and Williams, (England), Qualikems (India), Fluka (Germany), May and Baker (England). The commercial kits used for the assays were products of Randox, USA and Teco (TC), USA.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.2\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;Methods\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.2.1\u0026nbsp; \u0026nbsp;\u0026nbsp;Extraction Procedure\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe fresh stem-bark of \u003cem\u003eP. curatellifolia was\u003c/em\u003e cut into smaller sizes and air-dried to a constant weight for three months. The dried stem bark was then pulverized into powdered form with the aid of a mechanical grinder. Thereafter, the coarsely powdered (700 g) bark was subjected to cold maceration using absolute ethanol (2.5 L) for seventy-two hours in a 5 L transparent plastic container. It was intermittently stirred with the aid of a glass rod. Thereafter, it was filtered with a muslin cloth to separate the marc from the micelle. Further filtration was carried out using Whatman grade 1 qualitative filter papers. The filtrate was concentrated using vacuum evaporation and stored in a sterile screw-capped bottle and stored until needed for analysis.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.2.2\u0026nbsp; \u0026nbsp;\u0026nbsp;Determination of the Percentage Yield of the Ethanol Extract of \u003cem\u003eP. curatellifolia\u0026nbsp;\u003c/em\u003eStem-bark\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe percentage yield of the ethanol extract was determined from the weight of the dried pulverized stem-bark before maceration and the weight of the crude extract after concentration following the formula below;\u003c/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp\u003e\u003cimg src=\"data:image/png;base64,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\" width=\"506\" height=\"43\"\u003e\u003c/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.2.5 Acute Toxicity Studies\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eA total of 18 albino mice weighing 14-21 g were used to determine the acute toxicity and the median lethal dose of the extract using the method of Lorke (1983). The study was done in two phases, with both phases having three mice each in three groups. In phase 1, groups 1, 2, and 3 received 10 mg/kg b.w, 100 mg/kg b.w, and 1000 mg/kg b.w respectively. Groups 1, 2, and 3 in phase 2 received 1,600 mg/kg b.w, 2,900 mg/kg b.w and 5,000 mg/kg b.w of the extract respectively.\u003c/p\u003e\n\u003cp\u003eThe mice were then observed for 24 hours for lethality or any behavioural changes that shows signs of toxicity.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.2.6 Parasite Inoculation\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eFor inoculum preparation, donor mouse blood infected with \u003cem\u003eP. berghei\u003c/em\u003e was used. Blood was drawn from the donor mouse via heart puncture and serially diluted in normal saline to create a suspension containing approximately 1x10\u003csup\u003e6\u003c/sup\u003e infected RBCs per 0.2 ml suspension. This suspension (0.2 ml) was injected intraperitoneally into the experimental animals to initiate infection. Malaria infection was confirmed after 72 hours of inoculation.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.2.7\u0026nbsp; \u0026nbsp;\u0026nbsp;Experimental Design\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe inoculated animals (30 in number) were then randomly divided into six (6) groups of five (5) mice each and treated for four consecutive days (Peters \u003cem\u003eet al\u003c/em\u003e., 1975). On the fifth day, the tail tip of each mice was cut with razor and blood collected for determination of parasitemia. Thereafter, blood samples for hematological and biochemical studies were collected via ocular puncture into EDTA bottles and plain bottles respectively. The animals were subsequently sacrificed and the liver homogenate was used for further biochemical studies.\u003c/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eTable 1\u003c/span\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003e: Experimental design\u003c/span\u003e\u003c/p\u003e\n\u003ctable style=\"width:501.9pt;margin-left:-.05in;background:white;border-collapse:collapse;border:none;\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 73.5pt;border-width: 1pt medium;border-style: solid none;border-color: black currentcolor;padding: 0in 5.4pt;height: 18.35pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height: normal;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;color:black;'\u003eGroups\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 110.35pt;border-width: 1pt medium;border-style: solid none;border-color: black currentcolor;padding: 0in 5.4pt;height: 18.35pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height: normal;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;color:black;'\u003eDescription\u0026nbsp;\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 318.05pt;border-width: 1pt medium;border-style: solid none;border-color: black currentcolor;padding: 0in 5.4pt;height: 18.35pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height: normal;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;color:black;'\u003eTreatment\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 73.5pt;border: medium;padding: 0in 5.4pt;height: 20.6pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height: normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;color:black;'\u003eGroup 1\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 110.35pt;border: medium;padding: 0in 5.4pt;height: 20.6pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height: normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;color:black;'\u003eNormal control\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 318.05pt;border: medium;padding: 0in 5.4pt;height: 20.6pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height: normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;color:black;'\u003eAdministered with distilled water.\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 73.5pt;border: medium;padding: 0in 5.4pt;height: 31.5pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height: normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;color:black;'\u003eGroup 2\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 110.35pt;border: medium;padding: 0in 5.4pt;height: 31.5pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height: normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;color:black;'\u003ePositive control\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 318.05pt;border: medium;padding: 0in 5.4pt;height: 31.5pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height: normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;color:black;'\u003eAdministered with 0.2 ml \u003cem\u003ePlasmodium berghei-\u0026nbsp;\u003c/em\u003einfected blood and no treatment\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 73.5pt;border: medium;padding: 0in 5.4pt;height: 45pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height: normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;color:black;'\u003eGroup 3\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 110.35pt;border: medium;padding: 0in 5.4pt;height: 45pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height: normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;color:black;'\u003eStandard control\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 318.05pt;border: medium;padding: 0in 5.4pt;height: 45pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height: normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;color:black;'\u003eAdministered with 0.2 ml \u003cem\u003ePlasmodium berghei-\u003c/em\u003e infected blood and treated with 20/120 mg/kg b.w. of arthemeter/lumefantrine (standard drug)\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 73.5pt;border: medium;padding: 0in 5.4pt;height: 0.5in;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height: normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;color:black;'\u003eGroup 4\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 110.35pt;border: medium;padding: 0in 5.4pt;height: 0.5in;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height: normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;color:black;'\u003eTreatment group 1\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 318.05pt;border: medium;padding: 0in 5.4pt;height: 0.5in;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height: normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;color:black;'\u003eAdministered with 0.2 ml \u003cem\u003ePlasmodium berghei-\u003c/em\u003e infected blood and treated with 200 mg/kg b.w. of \u003cem\u003eP. curatellifolia\u003c/em\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 73.5pt;border: medium;padding: 0in 5.4pt;height: 0.5in;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height: normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;color:black;'\u003eGroup 5\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 110.35pt;border: medium;padding: 0in 5.4pt;height: 0.5in;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height: normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;color:black;'\u003eTreatment group 2\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 318.05pt;border: medium;padding: 0in 5.4pt;height: 0.5in;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height: normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;color:black;'\u003eAdministered with 0.2 ml \u003cem\u003ePlasmodium berghei-\u003c/em\u003e infected blood and treated with 400 mg/kg b.w. of \u003cem\u003eP. curatellifolia\u003c/em\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 73.5pt;border-width: medium medium 1pt;border-style: none none solid;border-color: currentcolor currentcolor black;border-image: none;padding: 0in 5.4pt;height: 0.5in;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height: normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;color:black;'\u003eGroup 6\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 110.35pt;border-width: medium medium 1pt;border-style: none none solid;border-color: currentcolor currentcolor black;border-image: none;padding: 0in 5.4pt;height: 0.5in;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height: normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;color:black;'\u003eTreatment group 3\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 318.05pt;border-width: medium medium 1pt;border-style: none none solid;border-color: currentcolor currentcolor black;border-image: none;padding: 0in 5.4pt;height: 0.5in;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height: normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;color:black;'\u003eAdministered with 0.2 ml \u003cem\u003ePlasmodium berghei-\u003c/em\u003e infected blood and treated with 600 mg/kg b.w. of \u003cem\u003eP. curatellifolia\u003c/em\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.2.8 Determination of Malaria parasitemia\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003emethod described by Dacie and Lewis (2011) was used to determine the malaria parasitemia (Mp+). The tip of the tail of each mouse was cut with a razor and gently squeezed to get a drop of blood unto the Centre of a microscopic slide. The blood was immediately spread on the slide with the aid of smooth-edged slide spreader to make a film of blood. The microscopic slide was then labelled at one end with black lead pencil and air dried in a horizontal position. The air-dried blood films were Placed on a staining rack, flooded with Leishman stain and left for 30 s to 1 min to fix. Thereafter, twice as much buffered distilled water (pH 7.2) from a plastic wash bottle was poured on the films, and then left to stain. After 10 minutes the stain was washed off with water. Finally, the back of the slides was wiped and placed in draining rack and allowed to air dry. After drying, the stained films were placed on counting chamber and viewed under the microscope at 100x magnification. The percentage parasitemia was determined from this.\u003c/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp\u003e\u003cimg src=\"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAgEAAABTCAYAAAAGP9S0AAAYPElEQVR4Xu2dWasc1deHKx/AOVcqQdSAuVKcIk4QIWpUQsCZiAiKM4LG2YiIs8YIgiMRRBwTBQlq1ICCE46goHjhcCHilbMfwP/7FPz6XWdlV1f18XTnnK5fQSCnu2oPz95da+211l570b//d1W+TMAETMAETMAEekdgkZWA3o25O2wCJmACJmACNQErAZ4IJmACJmACJtBTAlYCejrw7rYJmIAJmIAJWAnwHDABEzABEzCBnhKwEtDTgXe3TcAETMAETMBKgOeACZiACZiACfSUgJWAng68u20CJmACJmACVgI8B0zABEzABEygpwSsBPR04N1tEzABEzABE7AS4DlgAiZgAiZgAj0lYCWgpwPvbpuACZiACZiAlQDPARMwARMwARPoKQErAT0deHfbBEzABEzABKwEeA6YgAmYgAmYQE8JWAno6cC72yZgAiZgAiZgJcBzwARMwARMwAR6SsBKQE8H3t02ARMwARMwASsBngMmYAImYAIm0FMCVgJ6OvDutgmYgAmYgAlYCfAcMAETMAETMIGeErAS0NOBd7dNwARMwARMwEqA54AJmIAJmIAJ9JSAlYCeDry7bQImYAImYAJWAjwHTMAETMAETKCnBKwE9HTg3W0TMAETMAETsBLgOWACJmACJmACPSVgJaCnA+9um4AJmIAJmICVAM8BEzABEzABE+gpASsBPR14d9sEMoGTTz65euutt6o99tij+v333+uvP/jgg+q4446r///6669Xp5xyyljBLVq0aGJ1jdKRN954o7rtttuqzz//vH7s33//LT7Ofaeeemr1ww8/VPvvv/8oVdT3dq1n1ILPOeecasWKFdUll1wy6qOD+7uMzZ577ln98ccfg2eYS5dddll11113DT6jLS+99NKMdhx++OHVQw89VB177LEzPmf+3XnnnfW85OK+TZs2VQcffPCs++EHZxKwEuAZYQImUBP47bffquXLl9cCLAr8W265pf7sxRdfHDspKR1NQnbsDShUoDa9//771bJly2pG33///Zw3ZVz1/Pjjj9UBBxxQ0f4sZEfphNr366+/VnvttVfxUd0jJUh/ZwUSheKFF16oUAiYd1dccUWtYEWuzLdzzz23evzxx2vlhX6gBEhBHaXtvreZgJUAzw4TMIGawFdffVXdc889AxoS+pdffnl12mmn1VYAXthr166tV2bxxX7//fdXW7ZsqT777LPBS53VHvdwXXXVVQPlIv8drQvU+fTTT1cXXHBBRb1cW7dunSG84uqQleazzz5bt41V9HnnnVcLvDfffLPauHFjdffdd1c333zzjJVoHG7a/eSTT9ZtO+mkk6pHH310xgqe7++9997B6pZ7KLt0yZJy3333Vddff30rh9jvLvXAZv369XVb6ePbb789aCvj8tRTTw3aKmsOz8Axrs4lfOlDE0sphbfeemv12GOP1d2lTspljJuuJ554ol6px3uiwNc8O+SQQ2ZYSzQOUgKkuIilf6LjI2AlYHxsXbIJLCgCvMD/+uuvasmSJfUKTCu+Aw88sPrkk0/q1R/3HHXUUdUNN9xQnXDCCbWw40IAHnbYYbWwjffwHWZxhOsRRxxRCxJMxnfccUf9DJ/xnS4EFgLkoosuqld/3LP77rsPrBAIrdWrV9fPsIrkfgTGc889Vwv9VatW1e4LTNA8v3nz5ro/JTM4Fg4UlVdeeaU2L1MeV7Z4SBmJ7SwNLIJ48eLFgxV35EAf6Lc4nHnmmQN2se/8v1SPlCyZzBmTiy++eFAGHLlkZj/77LMHgjgqaLHdTSyl5MQyd9ttt7pvwxQqyoYhY8w8YFw2bNhQt4MyZT2AywMPPDBY9Ut505hSDswZB6/6x/8KsRIwfsauwQQWBAFe4DfddFMtEBFYWAVWrlxZnXjiiTuYvxFC+GolOCXg9DcdRojw4pdQQaBzRSGThWEWbgjqL774YvAM31933XXVGWecUSsmrPxpp4S8fPJtpm+sHqxG430Iy3feeWeHlb7qbPOna/WazeWZA3+XlICmelTul19+We2yyy7V9u3bq0svvbTib8ZKq/3vvvuuaKaPClqciMNYlspkRd8WF5JjAjDlM1bRfVCKCcjjRdtQZGIswYL4ES3ARloJWICD5iabwDgI8ALXyksr8nXr1lXvvffejNWphJL8vhKoMRhOq+IoNKIQyatm+lMSoggwWRJUD/dilj7yyCNrt0FUPFAaMJMPM1nzfDY/a/VJG6K5fxR/OoLzwQcfnFF35lDqd+x7SXlh5YzQ52KVjV/8yiuvHLhIIqPSvGBc5TLR920sc5lNCk6sL84DLAfETkRrhe6lPTfeeOPAiiGLTIwHYK4oFmAcc91l/j8BKwGeDSZgAnU8ACZ+CUAFdLEaW7NmzQxBK9+0XtoI4RzUlQP8JCC0SpYJOJp7sxDNArgUNIhQjatMhFd0UzQNbV71U87SpUtnWBV4VpaFLoGKJbdBDqZrKm9YPSULRex37nP8LnMXjzaWuUz6hnI1LCAym/lLwl3tkRWjiXGOI1DQ6jgCMvv+87cS0PcZsJP6j7mPf01BVpNslqKQ20zIk2zTpOtC0BAPEM2vjA+r+7zdLfqY9X8EMT7dTz/9tFYYsh86B4xJsMVnJGgw89MWFBBWvvLRS3BFMzhBhHEOdTFZw1YCi7q4CHZE0ETfNZ93tSxwrwQnMQj77rtvvVLPHEr9bqtHVgu1lQDAOFYxbgLFCW6KHYhKyLfffltt27atHuM2lrFMtZl2Eqj38ccfF2MssrtHik0OICXQMm5Bvfrqq2e4jcSSOIpHHnmkHh8CFLOVZtK/kWmtbyxKgKJkS9CIrs0mvGmF6341E8h+0p3JqqsfeWe2cZx1x1wAURHKKzu1QQFl/I0QR+ARjMdvmwA9FILsh0YxUNwAz4l5fEZzQrkKCO675pprZqz0EcpE/OMOoEwCDGUJ6LKFTX1A4CvyvVSW7msztcdxUdti8Fx+vtRvCT25PfJYxx0ZKEXZxM7q+vTTTx/scEBQax993PYJz8hrGEtZahizhx9+uG4S+Q/ieMV2oihgSZKioN0RBBNyobj9888/g5wTepb+EHMS28V3CHyUQuUH4D5iQdriMsb1O5H1C6WzaZslbWbsFZzZlPuANjIuBLKy84KdGzuzf2NRAjSIQKCDMgEyWYn6xXTIhGyLth3XgM5FufyAHLQyFyRnlmGuc8/UJc6OgPz3cUvd7Eoa/tSk6hlH26e9zLgtk742WQvlTtL2VO4l9wEKQekZlEOsZu+++26tsEmJ2hlbIsemBABBFoHoT9OEl3a4EDM/aW/tfDBlT9OP0FynaTQXfl+whLBboinqfq56OKl65qq9fSkHwcx14YUXDnJONCkBEuLRdaaYlpxbQharrFzifsNNMu75lsdv4kpAVA52htbzXyewzGvzxZ/9X/szX5431/kyEm4HBHBNYMVUDoFxUZlUPeNqf1/KlZBvUgKQB1w5cJHxxfIdt43i5sANkGNt5J5p24Y518wnrgTIbMIPLHYW7YgAEYAp37T8gWhUBMNgWsEnQ3BLzgSmyGI9H7egAC37YLK/hudfffXV6s8//6z9mvIXYt7Ri0BpK2P2LWl50cdIfSXfHZ/HDGV5MKNmKH+ssoO1+cPw4b322mu1JkkwTWx/3M9NnYoEj/m4b7/99kFe+MgCRQ1/I+XCFr9lzG5W8qdmfytbuWLCl1g+1pRhXGlv29x4+eWX66xvzA0uYk6UAY6x5JJpjnGJfr08t+Tv6zqecQyHxcLkse4SbT7XP3aXZwJ9IhBjKeJqXDEUvNO6JiMapgTIul3KJql3QlQepBjkd8DOcglMRAmQxhMDJxDC2surQzMQ8ggZ7YtV3ACDxmdoT3x2/PHH1y9yZShTBK+eL8EEvLKQKYIWoUbb2NNK5CzBTbSLQJVjjjmm/r2QjITgmLyHNQ84g809iuCV0IlbYfJEklkIwRTLpz8oGgTLcEmgN/kmmYS0H+GHRorApUwulCUuKVxSwugjwhgW/F8/CJUlFiQ14ULIskUIgUsAkPqlPsU9vWi6UkbUfkX2xvIzQyK782ejzA2CchjLo48+unr++efr+cJnjPH5558/GE/+ox9/nFvxh9plPPv0QnVfTWChEeA9etZZZ9W7GZRciWRLvJ8UwNq1T8OUAL3HS0pA6TkdxJSVAJUzaQv5RJQA4MQozxwNiuAiAjXm0pa2JAVCMLMglN8lWhW0stTpVaUkHorSjQGKDE5UTpggMt3EASsJK6VEzduZomBBmWBVHGMJlD1L5ZeSckjTzMpCnsASXNGEqX6qX2ITs6yJbe5jqT5+WAjXqEHDI07cUoYyOMZA0BLD0mdd5sawQ0ryeMrkFvta+qF2Gc+uLxDfZwImsPMI6J3KgoDsk3H3RNdWWQnoSirdVwoMzEXFzFWlaiREmwahJMByOaU0lbonam8lIdQkIJsOEpF5WVs/ohJQ8htloRSzg5V4DDMj5326el77vUuap1bM3Num6MT2oJhgFSAHOIpaVAKkePAZKUNLR6p2UQK6zo14+lrcvjNsPHO6WKwbJX/fsPEsKWFSdtt+MvPJHaCVSVub/b0JzBcCo/5+9A6crb/dSsAsR76LEtD0As9V/hcloEk45jr+ixIgHzImc3zwu+66a72vNgoW7b3VKlym+Jgju4tS0zQcTf3M46CYAMrhdLevv/66NvF3VQIU18B+ZYQ8FoNswoqnk2Ftufbaa2coA12UgK5zYxxKQJfxnOXPwo+ZgAlMmAALQd7NXWMAusof7tNipbQw1AI0W4Rx92ZFRgvAqXQHDNPaBLDk70ZI/vLLL4PMW6XVmoRmScPDNM8glNKaapB1D3/PVglAYCBoS9nN8uqStrByjgGQMd+AJkKOHKV9sa2l39AwJYA9qfwA4L1ixYoZ6VFHsXbErG5K0pLdAbFtCuqjP3HrSxcloOvcmGslYJTxnPC7zNWZgAmMSEDvPARvjNEapZi23QG8z3R8cyy3tBDWZ7ktbXWM0t5R7p1ITEA+VSs3EF85lxIn6PvoR24CJEGB7zem/ORzTLNkrsqBhiqfe2IKzNkqARJCMTiuJJhox08//TQ0yVBTf7S7YViCIiWgiNquAgFRPvDJi2MpYLGLJQBGWC7icatZCcgJf0qHsHRRAhinLnNjrpWAruM5yg/N95qACUyegBR60igT7DzbQ4naBLRW/PG9qliunBhP8ii3RdtFJ30+wtiUgLjtqw28crdru9l+++1XH+mJqVrBggrQK51nre+0LY+82uwU0BnomgisvlEWiHjnnnhEaQy+03NM2ZI5h8FiZcvJXGzLI5Ulx5JSNlvt/v7773q7IW2gvew0oB8S0gQH6uKwE67oO4/9QeAS8b5ly5a6vJJ/XWVJwxRvmbThq1W4JiDlchLZRx99VLNGYcIaQwrYvffeu7ZqMB7aFqg6JJRRKHB5fPjhh/UOhJgOWqlS5QLIiTSkFFBHZJ25UkeXuVHaodBUh8YzWp7EW9yiIjZsPCf/SnONJmACXQloi6ACARWYzc4Adm+xlToePjWs3NJ7I94vawPvNN7TvLPZ1cX7K79DJY+QIVu3bq0t3XovzzZmoSuT0n1jUQKa9ksPcwtoKxjAABn3tufymrZicDAFJh8EHIIpCswoEHWPJkHMnS5ImPHRHOMlX43O2mZSKRI/5uHWOewoBQh8bUfR/lTKZALEC4EbzeUxnwDlbNq0aZAPvGnA4UQ/EaYEJnJlFnHvrPbMo7QQv4DCsmrVqh36HV0a6jsMuZ9cDtQbE6tElwdtiDkZSqw1L0pceX7UubF+/fpiH0rjKQVITDXGXcbzv/zw/KwJmMD4CGjBFY9Q1oIi5n4Z1gLFTuFKjblheJ+hPOSMsTn/Cu9eMk6WsuLmPCRaQMYdcuOjM7PksSgBk2r8QquHFSdWgzwplD8hnhE+m751DYCcTdl+xgRMwARMYPoIWAmY0JiWchXEqll55hPTRm2alYBRifn+2RKQC6X0fM7N0FYHplDigWKcSdszfC83UNN23S5ljPOeaPVqi4saZzty2dkq2hSNXtqunK2SpW28cuvmA+Kol8yvstgqM6wPYpvk6O9Yl5WACfGXeRkTEdnr8KdzYYp/5plnqjVr1tTxB7O9+IEtX768djPMpxfObPvj5+Y3AVxO69atq+dsTA6lbJejnBDKHu58PG7X3lM3cTUEAM/HC0UFl9N8OmwMZmQVxR2KUMZ/3XREr+J7GE/eMfjSecco26uUMTKKKqCtdECO/ODcr9gm5gp1z9exm4/zaRxtshIwDqoNZbLSId2xEspIY8576EdtUsnPPum9pqO22fcvbAKsEiU42OmhgCZWhqSwlkKbV50xUloxMjE+Jn/PtmB+L/xWyDR66KGHzhAapYySIitLAbErpI9VWTG4WHEo+ImjYMt9IuW4tvaSJIvVLBcxTPnvGIuExYRAZwKRiVPKwbCUEY+r5Xv2s+uMDtpMeznJjrJg0RQ8Now19cR06WLUdCCOvifuKfrVFasUI9gZa+7LGUFz8G0MUF7Ys3+6Wm8lYLrG070xgYkSkALaZH3CYkAQlQ5ywloVD8Nqel67ixAwnKGhsz2iAJQpulQ35f7888/1llx21iBcWflu3LhxsCtI224VDKsA1dwmueoWL1482AWzcuXKir+x7JF3g909/F06HhZFhWDVZcuWVUuXLt0hRwftYwsbUeKyinD+Ran9rMybgoTbWEsRgEUpD0meOJmvkp0h7KPVUgoAnzFuGzZsqBUqbdnWTp22XWITnbiubEDASoAngwmYwKwJsDJEiMVVtArDWkB0dNz1ks33Tc+z6tWhU5RXEviUj0As1a026KwLtYG/49bguBLX6lYneMbVrrYQRwtbzI+h7+PKupQfA0HNFmVM4PqeveUcbLN9+/bBQTcKHs7tbxqoLqzVVx3w1TboOSaA2AuUmZiauxQTkGM0FJVvN2Ub8Z3zvZWAncPdtZrAVBAoHRaljkV/sj7L/n9Wj6xMc3AYAjauqkuBtU3PRrAIXdwIKj8KYd2HUOSSObtk3tZ5GBJk2Vqg7+M26FKbY7+ikIUBQXd5h1Buf9Ok6cKaZ7NyNWwSRg4lJYNn1Yd4ABr9iNYCFD3cJ7NN2TsVP5R53AkrAfN4cNw0E5jvBKLvPLc1r/olOGNWtexzVhmUmw/fimdscF80Qzdxiu0rrdZ5DsVEeUmaUlXnAL9shWDFjok97nBA4BLLIAVE8QeySpSCBmljTGCTOQxTAmKAZIk1z5aUoKYyI1+xy+4OuQViv3MdpTgCPhsWkDjf5/00tc9KwDSNpvtiAhMkoNVvU3BZFBAKAmRVngPI8PMfdNBBdQCchAnCDx8yvnYyr2Hyx4y+ZMmSOqslJmkJyG3bthW31+bVevybYL4c2LjPPvvUK1sSbdGnb775pq4foZwtHtkKIYUHXz6X/PusiukTda9evXqHeACi6smayUWkPgGEUhra4i3iUHdhLUHeJStdKRV3jDmAiVKS60A0bQHMZ7zkssRC2fImOGVdVYGAlQBPCxMwgZEJyJ/Ng6WDU/g8ZlBTSu+8HUzpWPEjx3PeYw4APt+8eXMdXR8j+5VmOgeqqTN5Bapgw5jpk3tjGwhgXLt2bUWWuFhutnhkK4S2AKt9OiFUqbfpfwyIpN6cvTNvkxwWb5EHrAtrCeMuQYHxeGmZ+uM2ZxSbUmZYxjGme1c75U5Q5j3uUybVkSefH5hTAlYC5hSnCzMBEzCB+UkAIc4e/UkfUDM/abhVImAlwHPBBEzABKacgJKJzTYp05Tj6XX3rAT0evjdeRMwgWknoNiN0gms095396+dgJWAdka+wwRMwARMwASmkoCVgKkcVnfKBEzABEzABNoJWAloZ+Q7TMAETMAETGAqCVgJmMphdadMwARMwARMoJ2AlYB2Rr7DBEzABEzABKaSgJWAqRxWd8oETMAETMAE2glYCWhn5DtMwARMwARMYCoJWAmYymF1p0zABEzABEygnYCVgHZGvsMETMAETMAEppKAlYCpHFZ3ygRMwARMwATaCVgJaGfkO0zABEzABExgKglYCZjKYXWnTMAETMAETKCdgJWAdka+wwRMwARMwASmkoCVgKkcVnfKBEzABEzABNoJWAloZ+Q7TMAETMAETGAqCVgJmMphdadMwARMwARMoJ2AlYB2Rr7DBEzABEzABKaSgJWAqRxWd8oETMAETMAE2glYCWhn5DtMwARMwARMYCoJWAmYymF1p0zABEzABEygncD/ANvMGzS6EvMJAAAAAElFTkSuQmCC\" width=\"513\" height=\"83\"\u003e\u003c/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.2.9 Statistical Analysis\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe results were expressed as Mean \u0026plusmn; standard deviation and test of statistical significance was carried out using one-way analysis of variance (ANOVA). The statistical package used was the Statistical Product and Service Solutions (SPSS) version 21, the result being considered significant at p \u0026lt; 0.05.\u003c/p\u003e"},{"header":"Results","content":"\u003cp\u003e\u003cstrong\u003e3.1\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;Percentage Yield of Ethanol Extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e Stem-Bark\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eSeven hundred grams (700 g) of the coarsely powdered \u003cem\u003eParinari curatellifolia\u003c/em\u003e stem bark yielded 57.57 g of extract which is 8.23% extract after extraction with absolute ethanol.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e3.2\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;Qualitative Phytochemical Screening of Ethanol Extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e Stem Bark\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe phytochemical screening carried out on the ethanol extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e stem bark showed it contains the secondary metabolites as shown in Table 3.\u003c/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eTable 2: Secondary metabolites found in ethanol extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e stem bark\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;Phytochemical constituents \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;Presence\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;Tannin \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;+++\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;Phenolics\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;+++\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;Saponin\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;++\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;Flavonoids\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;++\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;Steroids\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;+\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;Glycosides\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;++\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;Terpenoids\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;++\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;Alkaloid\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;++\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u0026nbsp;Key: Highly present \u0026nbsp; \u0026nbsp; \u0026nbsp;+++ \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;Moderately present \u0026nbsp; \u0026nbsp; \u0026nbsp;++ \u0026nbsp; \u0026nbsp; \u0026nbsp; Slightly present \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;+\u003c/span\u003e\u003c/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e3.3\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;Quantitative Phytochemical Composition of Ethanol Extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e Stem Bark\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe table\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003ebelow shows the quantitative phytochemical composition of ethanol extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e stem bark.\u003c/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eTable 3. Quantitative phytochemical constituents of ethanol extract of \u003cem\u003eParinari curatellifolia\u0026nbsp;\u003c/em\u003estem bark\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003ePhytochemical constituent \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; Concentration (mg/100g)\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-indent:.5in;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eTannin \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;1771.69\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-indent:.5in;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003ePhenols\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;1281.99\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-indent:.5in;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eSaponin\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;123.10\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-indent:.5in;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eFlavonoids\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;493.83\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-indent:.5in;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eSteroid\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;51.58\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:.5in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGlycosides\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;645.68\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-indent:.5in;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eTerpenoids \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;246.23\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-indent:.5in;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eAlkaloids\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;709.59\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:8.0pt;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;'\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e3.4 Acute Toxicity of Ethanol Extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e Stem Bark\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe result of the\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003eacute toxicity test of ethanol extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e stem bark showed there was no mortality recorded as a result of the extract at the doses tested, ranging from 10 mg/kg b.w to 5000 mg/kg b.w as can be seen in Table 4.\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eTable 4. Phases I \u0026amp; II of acute toxicity test of ethanol extract of \u003cem\u003eParinari curatellifolia\u0026nbsp;\u003c/em\u003estem bark extract\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;Phase/Groups \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; Dosage of extract (mg/kg b.w) \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;Mortality\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;text-indent:.5in;line-height:normal;'\u003e\u003cstrong\u003e\u003cspan style='font-family:\"Times New Roman\",serif;'\u003ePhase I\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;text-indent:.5in;line-height:normal;'\u003e\u003cspan style='font-family:\"Times New Roman\",serif;'\u003eGroup 1\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;10\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;0/3\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;text-indent:.5in;line-height:normal;'\u003e\u003cspan style='font-family:\"Times New Roman\",serif;'\u003eGroup 2\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;100\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;0/3\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;text-indent:.5in;line-height:normal;'\u003e\u003cspan style='font-family:\"Times New Roman\",serif;'\u003eGroup 3\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;1000\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;0/3\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;text-indent:.5in;line-height:normal;'\u003e\u003cstrong\u003e\u003cspan style='font-family:\"Times New Roman\",serif;'\u003ePhase II\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;text-indent:.5in;line-height:normal;'\u003e\u003cspan style='font-family:\"Times New Roman\",serif;'\u003eGroup 1\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;1600\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;0/3\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;text-indent:.5in;line-height:normal;'\u003e\u003cspan style='font-family:\"Times New Roman\",serif;'\u003eGroup 2\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;2900\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;0/3\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;text-indent:.5in;line-height:normal;'\u003e\u003cspan style='font-family:\"Times New Roman\",serif;'\u003eGroup 3\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;5000\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;0/3\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003c/span\u003e\u003c/strong\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003en = 3\u003c/span\u003e\u003c/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e3.5\u003c/strong\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003cstrong\u003eEffect of Ethanol Extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e Stem Bark on the Percentage parasitemia of \u003cem\u003ePlasmodium berghei\u003c/em\u003e-infected Mice\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eTable 5 shows the percentage parasitemia of the mice treated with the ethanol extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e stem bark. Before the treatment, the percentage parasitemia of the mice that were infected with malaria (groups 2 \u0026ndash; 6) were significantly higher (p \u0026lt; 0.05) than non-infected ones (normal control). There was non-significant (p \u0026gt; 0.05) difference among all the infected groups. After the treatment however, the percentage parasitemia in groups 3 to 6, were significantly lower (p \u0026lt; 0.05) compared with group 2 (untreated group). The treatment produced non-significant (p \u0026gt; 0.05) difference in the percentage parasitemia of the treated groups.\u003c/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eTable 5: Percentage parasitemia of \u003cem\u003ePlasmodium berghei\u003c/em\u003e-infected mice treated with ethanol extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e stem bark\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup\u0026nbsp;\u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; Parasitemia (%)\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;Before treatment\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;After treatment \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;Reduction\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003e1\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;0.00 \u0026plusmn; 0.00\u003csup\u003ea\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;0.00 \u0026plusmn; 0.00\u003csup\u003ea\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; 0.00 \u0026plusmn; 0.00\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003e2\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;70.20 \u0026plusmn; 10.62\u003csup\u003eb\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;103.00 \u0026plusmn; 3.32\u003csup\u003ec\u003c/sup\u003e\u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;- 32.80 \u0026plusmn; 10.47\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003e3\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;69.80 \u0026plusmn; 11.30\u003csup\u003eb\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;6.40 \u0026plusmn; 1.95\u003csup\u003eb\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;63.40 \u0026plusmn; 11.33\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003e4\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;76.20 \u0026plusmn;\u003c/span\u003e \u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003e6.87\u003csup\u003eb\u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;8.40 \u0026plusmn; 2.07\u003csup\u003eb\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;67.80 \u0026plusmn; 7.19\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003e5\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;74.00 \u0026plusmn; 3.67\u003csup\u003eb\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;6.40 \u0026plusmn; 1.67\u003csup\u003eb\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;67.60 \u0026plusmn; 3.21\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003e6\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;78.00 \u0026plusmn; 5.66\u003csup\u003eb\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;5.40 \u0026plusmn; 2.51\u003csup\u003eb\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;72.60 \u0026plusmn; 6.87 \u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eResults are expressed in Mean \u0026plusmn; SD \u0026nbsp;(n = 5)\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eMean values with different letters as superscript down the column are considered significant at P \u0026lt; 0.05.\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 1 : Normal control (Unparasitized and treated with distilled water)\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 2: Positive control (Parasitized and untreated)\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 3: Standard control (Parasitized and treated with 20/120 mg/kg b.w Artemether/lumefantrine)\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 4: Test group (Parasitized and treated with 200 mg/kg b. wt of EEPCSB)\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 5: Test group (Parasitized and treated with 400 mg/kg b.w of EEPCSB)\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 6: Test group (parasitized and treated with 600 mg/kg b.w of EEPCSB)\u003c/span\u003e\u003c/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp\u003eIn this study, the extract significantly (p \u0026lt; 0.05) reduced the percentage parasitemia of the treated animals compared to the untreated group. This corroborates the earlier report of the antiplasmodial activity of \u003cem\u003eParinari curatellifolia\u003c/em\u003e stem bark extract\u0026nbsp;(Omoniwa \u003cem\u003eet al\u003c/em\u003e., 2021). This antimalarial effect of this extract could be attributed to the activities of bioactive compounds found in it, most likely the alkaloids, terpenoids and/or saponins. These compounds, acting singly or in synergy could have exerted a cytotoxic effect on the parasite. \u0026nbsp;This result shows that \u003cem\u003eParinari curatellifolia\u003c/em\u003e stem bark is effective in the managing of malarial and can serve as source of a lead compound for a novel antimalarial drug.\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e3.6\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;Effect of Ethanol Extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e Stem Bark on Some Hematological Parameters in \u003cem\u003ePlasmodium berghei\u003c/em\u003e-infected Mice\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eTable 6 below shows the effect of the ethanol extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e stem bark on mice induced with malaria.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp\u003e\u003cimg 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\" height=\"374\" width=\"647\"\u003e\u003c/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e3.7\u003c/strong\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003cstrong\u003eEffect of Ethanol Extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e Stem Bark on Some Liver Function Parameters of \u003cem\u003ePlasmodium berghei\u003c/em\u003e-infected Mice\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eIn the Table 7 below, the result of the liver function parameters of malaria infected mice treated with ethanol extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e stem bark (EEPCSB) is shown.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eTable 7: Liver function parameters of \u003cem\u003ePlasmodium berghei\u003c/em\u003e infected mice treated with ethanol extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e.\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;line-height:normal;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eTreatment \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; Liver Function Parameters\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;line-height:normal;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;AST activity \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; ALT activity \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;ALP activity \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; Bilirubin Conc.\u003cbr\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; (IU/L) \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;(IU/L) \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; (IU/L) \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;(mg/dl)\u003cbr\u003e\u0026nbsp;\u003c/span\u003e\u003c/strong\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 1 \u0026nbsp; \u0026nbsp; \u0026nbsp;19.00 \u0026plusmn; 1.22\u003csup\u003ea\u003c/sup\u003e \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 29.80 \u0026plusmn; 1.48\u003csup\u003ea\u0026nbsp;\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; 43.20 \u0026plusmn; 2.59\u003csup\u003ea\u0026nbsp;\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; 4.74 \u0026plusmn; 0.70\u003csup\u003ea\u003c/sup\u003e\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 2 \u0026nbsp; \u0026nbsp; \u0026nbsp;42.20 \u0026plusmn; 1.79\u003csup\u003ec\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 66.68 \u0026plusmn; 2.41\u003csup\u003ec\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 86.00 \u0026plusmn; 4.74\u003csup\u003ed\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp;8.02 \u0026plusmn; 0.70\u003csup\u003eb\u003c/sup\u003e\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 3 \u0026nbsp; \u0026nbsp; \u0026nbsp;23.20 \u0026plusmn; 2.28\u003csup\u003eb\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 34.80 \u0026plusmn; 3.03\u003csup\u003eb\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 49.60 \u0026plusmn; 1.52\u003csup\u003ebc\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp;4.66 \u0026plusmn; 0.60\u003csup\u003ea\u003c/sup\u003e\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 4 \u0026nbsp; \u0026nbsp; \u0026nbsp;23.60 \u0026plusmn; 2.61\u003csup\u003eb\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 32.20 \u0026plusmn; 1.78\u003csup\u003eab\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 46.20 \u0026plusmn; 1.48\u003csup\u003eab\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp;4.49 \u0026plusmn; 0.19\u003csup\u003ea\u003c/sup\u003e\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 5 \u0026nbsp; \u0026nbsp; \u0026nbsp;21.60 \u0026plusmn; 1.52\u003csup\u003eb\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 32.20 \u0026plusmn; 2.74\u003csup\u003eab\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 47.40 \u0026plusmn;\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e4.78\u003csup\u003eabc\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp;4.60 \u0026plusmn; 0.61\u003csup\u003ea\u003c/sup\u003e\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 6 \u0026nbsp; \u0026nbsp; \u0026nbsp;23.20 \u0026plusmn; 1.92\u003csup\u003eb\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 30.40 \u0026plusmn; 2.30\u003csup\u003ea\u003c/sup\u003e \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 50.80 \u0026plusmn; 1.64\u003csup\u003ec\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp;4.69 \u0026plusmn; 0.61\u003csup\u003ea\u003c/sup\u003e\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/span\u003e\u003c/strong\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eResults are expressed in Means \u0026plusmn; Standard Deviation (n = 5)\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eMean values with different letters as superscript down the column are considered significant at p \u0026lt; 0.05.\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 1: Normal control (Unparasitized and treated with distilled water)\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 2: Positive control (Parasitized and untreated)\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 3: Standard control (Parasitized and treated with 20/120 mg/kg b.w Artemether/lumefantrine)\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 4: Test group (Parasitized and treated with 200 mg/kg b. wt of EEPCSB)\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 5: Test group (Parasitized and treated with 400 mg/kg b.w of EEPCSB)\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 6: Test group (parasitized and treated with 600 mg/kg b.w of EEPCSB)\u003c/span\u003e\u003c/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e3.8\u003c/strong\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003cstrong\u003eEffect of Ethanol Extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e Stem Bark on the Lipid Profile of \u003cem\u003ePlasmodium berghei\u003c/em\u003e-infected Mice\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe lipid profile of the malaria infected mice treated with ethanol extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e is shown in the Table 8 below.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eTable 8: Lipid profile of \u003cem\u003ePlasmodium berghei\u003c/em\u003e infected mice treated with ethanol extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e stem bark\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;line-height:normal;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eTreatment \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; Lipid \u0026nbsp;Profile\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;line-height:normal;'\u003e\u003cstrong\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;Total Cholesterol \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; TAG Conc. \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; HDL Conc. \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; LDL Conc.\u003cbr\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; (mg/dl) \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;(mg/dl) \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; (mg/dl) \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;(mg/dl)\u003cbr\u003e\u0026nbsp;\u003c/span\u003e\u003c/strong\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 1 \u0026nbsp; \u0026nbsp; 94.00 \u0026plusmn; 3.16\u003csup\u003ea\u003c/sup\u003e \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 104.80 \u0026plusmn; 3.03\u003csup\u003ea\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; 84.00 \u0026plusmn; 3.16\u003csup\u003ee\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; 20.20 \u0026plusmn; 2.28\u003csup\u003ea\u003c/sup\u003e\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 2 \u0026nbsp; \u0026nbsp; 116.20 \u0026plusmn; 4.60\u003csup\u003ed\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 130.00 \u0026plusmn; 6.20\u003csup\u003ec\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 33.20 \u0026plusmn; 2.28\u003csup\u003ea\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp;65.00 \u0026plusmn; 3.16\u003csup\u003ec\u003c/sup\u003e\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 3 \u0026nbsp; \u0026nbsp; 105.40 \u0026plusmn; 2.41\u003csup\u003ec\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 113.60 \u0026plusmn; 3.21\u003csup\u003eb\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 64.20 \u0026plusmn; 1.79\u003csup\u003eb\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp;25.00 \u0026plusmn; 2.24\u003csup\u003eb\u003c/sup\u003e\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 4 \u0026nbsp; \u0026nbsp; 98.40 \u0026plusmn; 4.93a\u003csup\u003eb\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 111.60 \u0026plusmn; 3.29\u003csup\u003eb\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 69.20 \u0026plusmn; 5.54\u003csup\u003ebc\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp;26.40 \u0026plusmn; 3.05\u003csup\u003eb\u003c/sup\u003e\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 5 \u0026nbsp; \u0026nbsp; 103.00 \u0026plusmn; 4.36\u003csup\u003ebc\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 109.00 \u0026plusmn; 2.12\u003csup\u003eab\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 73.20 \u0026plusmn;\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e4.76\u003csup\u003ecd\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp;24.60 \u0026plusmn; 2.19\u003csup\u003eb\u003c/sup\u003e\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 6 \u0026nbsp; \u0026nbsp; 100.00 \u0026plusmn; 3.54\u003csup\u003eb\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp;108.460 \u0026plusmn; 2.19\u003csup\u003eab\u003c/sup\u003e \u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 75.60 \u0026plusmn; 4.83\u003csup\u003ed\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp;26.00 \u0026plusmn; 4.18\u003csup\u003eb\u003c/sup\u003e\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eResults are expressed in Means \u0026plusmn; Standard Deviation (n = 5)\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eMean values with different letters as superscript down the column are considered significant at p \u0026lt; 0.05.\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 1: Normal control (Unparasitized and treated with distilled water)\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 2: Positive control (Parasitized and untreated)\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 3: Standard control (Parasitized and treated with 20/120 mg/kg b.w Artemether/lumefantrine)\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 4: Test group (Parasitized and treated with 200 mg/kg b. wt of EEPCSB)\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 5: Test group (Parasitized and treated with 400 mg/kg b.w of EEPCSB)\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;font-size:11.0pt;font-family:\"Calibri\",sans-serif;text-align:justify;line-height:normal;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003eGroup 6: Test group (parasitized and treated with 600 mg/kg b.w of EEPCSB)\u003c/span\u003e\u003c/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e3.9\u003c/strong\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u003cstrong\u003eEffect of Ethanol Extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e Stem Bark on the Malondialdehyde Concentration and Antioxidant Markers in \u003cem\u003ePlasmodium berghei\u003c/em\u003e-infected Mice\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eTable 9 shows the antioxidant markers and the malondialdehyde concentration of the \u003cem\u003ePlasmodium berghei\u003c/em\u003e-infected mice treated with ethanol extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e stem bark.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp\u003eTable 9: Malondialdehyde concentration and antioxidant markers of \u003cem\u003ePlasmodium berghei\u003c/em\u003e infected mice treated with ethanol extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e stem bark\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eTreatment \u0026nbsp; \u0026nbsp; MDA Conc. \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;SOD activity \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;Catalase \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; GSH Conc.\u003cbr\u003e\u0026nbsp; group \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; (mg/dl) \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;(IU/L) \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; (IU/L) \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;(mg/dl)\u003cbr\u003e\u0026nbsp;\u003c/strong\u003eGroup 1 \u0026nbsp; \u0026nbsp; \u0026nbsp;3.79 \u0026plusmn; 0.90\u003csup\u003ea\u003c/sup\u003e \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 8.64 \u0026plusmn; 0.22\u003csup\u003ec\u0026nbsp;\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; 0.58 \u0026plusmn; 0.32\u003csup\u003ebc\u0026nbsp;\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; 3.07 \u0026plusmn; 0.60\u003csup\u003eabc\u003c/sup\u003e\u003c/p\u003e\n\u003cp\u003eGroup 2 \u0026nbsp; \u0026nbsp; \u0026nbsp;8.85 \u0026plusmn; 0.43\u003csup\u003ec\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;3.11 \u0026plusmn; 0.73\u003csup\u003ea\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 0.23 \u0026plusmn; 0.11\u003csup\u003ea\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp;5.42 \u0026plusmn; 0.57\u003csup\u003ed\u003c/sup\u003e\u003c/p\u003e\n\u003cp\u003eGroup 3 \u0026nbsp; \u0026nbsp; \u0026nbsp;4.97 \u0026plusmn; 0.72\u003csup\u003eb\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 7.32 \u0026plusmn; 1.24\u003csup\u003eb\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 0.51 \u0026plusmn; 0.07\u003csup\u003eb\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp;3.66 \u0026plusmn; 0.31\u003csup\u003ec\u003c/sup\u003e\u003c/p\u003e\n\u003cp\u003eGroup 4 \u0026nbsp; \u0026nbsp; \u0026nbsp;4.63 \u0026plusmn; 0.02\u003csup\u003eab\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 8.26 \u0026plusmn; 0.57\u003csup\u003eb\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 0.58 \u0026plusmn; 0.03\u003csup\u003ebc\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp;3.41 \u0026plusmn; 0.73\u003csup\u003ebc\u003c/sup\u003e\u003c/p\u003e\n\u003cp\u003eGroup 5 \u0026nbsp; \u0026nbsp; \u0026nbsp;4.80 \u0026plusmn; 0.96\u003csup\u003eb\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 8.51 \u0026plusmn; 0.85\u003csup\u003ec\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 0.60 \u0026plusmn;\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e0.05\u003csup\u003ec\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp;2.81 \u0026plusmn; 0.67\u003csup\u003eab\u003c/sup\u003e\u003c/p\u003e\n\u003cp\u003eGroup 6 \u0026nbsp; \u0026nbsp; \u0026nbsp;4.79 \u0026plusmn; 0.55\u003csup\u003eb\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 8.74 \u0026plusmn; 0.44\u003csup\u003ec\u003c/sup\u003e \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp; 0.56 \u0026plusmn; 0.04\u003csup\u003ebc\u003c/sup\u003e\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp; \u0026nbsp; \u0026nbsp;2.35 \u0026plusmn; 0.21\u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u0026nbsp; \u0026nbsp;\u0026nbsp;\u003c/strong\u003eResults are expressed in Means \u0026plusmn; Standard Deviation (n = 5)\u003c/p\u003e\n\u003cp\u003eMean values with different letters as superscript down the column are considered significant at p \u0026lt; 0.05.\u003c/p\u003e\n\u003cp\u003eGroup 1: Normal control (Unparasitized and treated with distilled water)\u003c/p\u003e\n\u003cp\u003eGroup 2: Positive control (Parasitized and untreated)\u003c/p\u003e\n\u003cp\u003eGroup 3: Standard control (Parasitized and treated with 20/120 mg/kg b.w Artemether/lumefantrine)\u003c/p\u003e\n\u003cp\u003eGroup 4: Test group (Parasitized and treated with 200 mg/kg b. wt of EEPCSB)\u003c/p\u003e\n\u003cp\u003eGroup 5: Test group (Parasitized and treated with 400 mg/kg b.w of EEPCSB)\u003c/p\u003e\n\u003cp\u003eGroup 6: Test group (parasitized and treated with 600 mg/kg b.w of EEPCSB)\u003c/p\u003e"},{"header":"Discussion","content":"\u003cp\u003eThe phytochemical screening of the ethanol extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e showed the presence of saponins, phenolics, flavonoids, alkaloids, glycosides, tannins, terpenoids and steroids. The quantity of steroids detected was negligible. Alkaloids, saponins, flavonoids, terpenoids, and glycosides were detected in moderate to high amounts. Our result was in agreement with that of (Halilu \u003cem\u003eet al.\u003c/em\u003e, 2008) which reported detection of tannins, saponins, flavonoids, steroids, glycosides in methanolic extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e stem bark, but showed no detection of alkaloids. Furthermore, our findings is also comparable to those of (Sylvanus \u003cem\u003eet al.\u003c/em\u003e, 2014), differing only in the absence of flavonoids in ethanolic stem bark extract of Parinari curatellifolia, of their report. The report of Issakou \u003cem\u003eet al\u003c/em\u003e (2022) also showed the detection tannins, saponins, alkaloids, flavnoids, terpenoids, and steroids which is in tandem with our result. In addition to phenolics, tannins, saponins, glycosides, alkaloids, terpenoids, flavonoids and steroids, (Omoniwa \u003cem\u003eet al\u003c/em\u003e., 2021) reported the detection of resins in ethanolic extract of \u003cem\u003eP.curatellifolia\u003c/em\u003e stem bark, which was not included in our study.\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eThe plant extract\u0026apos;s wide range of secondary metabolites may explain why it is used in ethnomedicine to treat a number of illnesses in Africa, such as malaria, fever, toothaches, fractures, pneumonia, cancer, inflammation, bacterial infections, and diabetes\u0026nbsp;( Halilu \u003cem\u003eet al\u003c/em\u003e., 2008; Kuntze \u003cem\u003eet al\u003c/em\u003e., 2017;\u0026nbsp;Mawire \u003cem\u003eet al\u003c/em\u003e., 2021). Alkaloids are class of small nitrogen- containing organic molecules found in plants. Many alkaloids are great therapeutic agents which are used to treat various diseases including malaria, diabetes, cardiac dysfunction e.tc\u0026nbsp;(Ain \u003cem\u003eet al\u003c/em\u003e., 2016). Quinine, an alkaloid isolated from the bark of cinchona tree is an antimalarial drug. Isoquinoleine alkaloid extract from \u003cem\u003eFumaria capreolata\u003c/em\u003e, exerted antioxidant, analgesic, and intestinal anti-inflammatory activities in DNBS model of experimental colitis in mice. Antitumor efficacy is demonstrated by the alkaloids zephyrantine and narcyclasine-glucoside (Noureddine Bribi, 2018). Vincristine, an alkaloid from Vinca rosea, is an anticancer medicine, sanguinaria from Sanguinaria canadensis is antibacterial, and ementine from Carapichea ipecacuanha is antiprotozoal (Roy, 2017; Heinrich et al., 2021).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eFlavonoids have been reported to possess antioxidant effect, anti-bacterial effect, anti-cancer effect \u0026nbsp;cardioprotective effect, anti-inflammatory effect, and immune system promoting effect\u0026nbsp;(Tungmunnithum \u003cem\u003eet al\u003c/em\u003e., 2018). Phenolics and flavonoids are the largest secondary metabolites from plants with antioxidant properties. Chrysosplenetin, a known polymethoxylated flavonoids in \u003cem\u003eArtemisia annua L\u003c/em\u003e. and other Chinese herbal plants, was reported to boost the antimalarial activity of Artemisinin when co-administered\u0026nbsp;(Patel, 2022). Many flavonoids including triglycoside of kaempferide, kaempferol-O-rutinoside, kaempferol-3-O-b-D-glucopyranosyl isoorientin, isovitexin, vitexin, 5,6-dimethoxyflavone, 2-hydroxyisoflavanone, naringenin, naringenin 7-O-b-D-glucoside, 5-hydroxy-7,8-dimethoxyflavanone 5-rhamnoside, kaempferol 3-rhamnoside-7-xylosyl-(1-2)-rhamnoside have been found to possess antimicrobial activities\u0026nbsp;(Mierziak \u003cem\u003eet al\u003c/em\u003e., 2014).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003ePlant tannins are polyphenols that are generically classed as hydrolyzed and condensed tannins. They have been reported to possess antioxidant, anti-bacterial, anti-inflammatory, antiparasitic and antiviral activities\u0026nbsp;(Tong \u003cem\u003eet al\u003c/em\u003e., 2022).\u0026nbsp;Saponins are naturally occurring surface-active glycosides with a particular foaming property that are present in both wild and cultivated plants as steroidal and triterpenoid saponins, respectively.\u0026nbsp;They are reported to possess a variety of biological activities including membrane-permeabiliting, immunostimulant, hypocholesterolaemic and anticarcinogenic, antiparasitic, antiprotozoal, cytotoxic, antimicrobial and antioxidant\u0026nbsp;(Guclu-Ustundag and Mazza, 2007; Desai \u003cem\u003eet al\u003c/em\u003e., 2009). \u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eThe acute toxicity study of ethanol extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e stem bark indicated that the median lethal dose (LD\u003csub\u003e50\u003c/sub\u003e) is above 5000 mg/kg b.w, as there was no mortality recorded due to the extract in mice up to 72 hours in all the doses tested. This indicates that the extract is safe for comsumption for both animals and humans. Ogbonnia et al 2008 had earlier reported the estimated LD\u003csub\u003e50\u003c/sub\u003e of ethanol extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e seeds to be 7.2 g/kg b.w,which supports our result. However, (Atawodi \u003cem\u003eet al\u003c/em\u003e., 2103) reported that the LD\u003csub\u003e50\u003c/sub\u003e of methanolic root extract of \u003cem\u003eP. curatellifolia\u003c/em\u003e was 1,265 mg/kg b.w in rats. It could therefore be inferred that stem bark is safer compared to the root extract.\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eThe percentage parasitemia of the animals treated with the extract in this study was significantly (p\u0026nbsp;\u0026lt;\u0026nbsp;0.05) reduced compared to the untreated group. This result is in tandem with that of (Omoniwa \u003cem\u003eet al.\u003c/em\u003e, 2021) which had earlier reported the\u003cem\u003e\u0026nbsp;in \u0026nbsp;vitro\u003c/em\u003e antiplasmodial activity of ethanol extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e stem bark. The observed antimalarial effect of this extract is attributable to the activities of the constituent bioactive compounds including alkaloids, terpenoids, and/or saponins. These compounds could have acted singly or in synergy to exert cytotoxic effect on the malarial parasite. Further studies of the compounds in the extract could lead to isolation of lead compounds for novel antimalarial drug.\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eThe result of this study indicated that the hematological parameters including packed cell volume (PCV), red blood cell (RBC) count, and haemoglobin (Hgb) concentration of the treated animals were significantly (p \u0026lt; 0.05) increased compared to the untreated animals, while the white blood cell (WBC) count of the treated animals was significantly (p \u0026lt; 0.05) lower compared to the untreated group. The observed decrease in the PCV, RBC count and Hgb concentration of the untreated infected animals was in accord with previous studies (Francis et al., 2014; Nefsu and Amsalu, 2019), which adduced that the phagocytosis of parasite-infected RBC, decreased RBC formation in the bone marrow, and mechanical destruction of the parasitized red blood cells accounted for the decline. In addition, the observed rise in the WBC count of the untreated infected mice was consistent with past studies (Francis \u003cem\u003eet al\u003c/em\u003e., 2014; Kotepui \u003cem\u003eet al\u003c/em\u003e., 2015; Sirak \u003cem\u003eet al\u003c/em\u003e., 2016), which all suggested that such increase was attributable to enhanced leucocyte release in response to plasmodium infection. Hemolysis and anaemia are known features of malaria, due to the invasion of the red blood cells and digestion of heamoglobin by plasmodium parasite (Kavishe et al., 2017; Mawson, 2013). This accounts for the reduced PCV, RBC count and Hgb concentration in malarial infected subjects as observed in the untreated infected animals.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eHowever, there was an increase in the PCV, RBC count, Hgb concentration and a decrease in WBC count of the animals when treated with the extract, indicating a reversal of the hematological aberrations caused by the Plasmodium parasites. This result suggests that the extract possesses antiplasmodial activity, leading to drastic reduction in parasitemia and hemolysis in the treated animals. The erythrocytic schizonts attack the red blood cells, damaging the membrane through the byproducts of its haemoglobin metabolism such as ferriprotoporphyrin 9, which exert oxidative stress and lipid peroxidation to the red blood cells, causing hemolytic anemia in malaria disease. Our result also agrees with the report of (Ajibola \u003cem\u003eet al\u003c/em\u003e., 2012) which indicated that non-malaria infected persons has higher PCV and Hgb concentration than malaria patients. Sirak \u003cem\u003eet al\u003c/em\u003e. (2016) had also reported that density of parasitemia determined the changes in hematological parameters in malaria patients. Patients with high malaria parasite density had lower PCV and Hgb concentration with higher WBC count while patients with low parasitemia had higher PCV and Hgb concentration with lower WBC count.\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eAnemia is determined by estimating the packed cell volume. A PCV value of less than 30% indicates anemia\u0026nbsp;(Osonuga \u003cem\u003eet al\u003c/em\u003e., 2006). The reduced PCV indicated anemia in the infected animals. Anemia is a common and frequently severe complication of malaria, particularly in young children and pregnant women, and accounts for more than 50% of malaria-associated mortality in endemic areas\u0026nbsp;(Mohandas and An, 2013). Malaria-induced anemia is caused by multiple factors including lysis of parasitized erythrocytes, immune-mediated hemolysis, dyserythropoietic changes and shortened life span of erythrocytes\u0026nbsp;(Osonuga \u003cem\u003eet al\u003c/em\u003e., 2006)\u0026nbsp; The ethanol extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e was able to restore the PCV of the treated animals in groups 5 and 6 to a level that is statistically non-significantly different compared to the normal control. This also demonstrates that the extract was able to stop hemolysis of red blood cells and may have the ability to increase erythrocyte synthesis in mice. This finding also indicates that the extract reversed anemia in mice to a comparably the same level as artemether/lumefantrine.\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eThe clinical symptoms and pathologies associated with malaria are as a result of cellular and membrane changes in the human red blood cells (RBC) induced by the intra-erythrocytic parasite. These structural and biochemical changes, mediated by interactions between red cell membrane and parasite-expressed proteins, impart rigidity and adhesiveness to the infected red blood cells (iRBC) making them adhesive for a variety of cell types, including vascular endothelial cells (cytoadherence), platelets, and other infected or uninfected red cells. Infected red cells are eventually ruptured by the matured intra-erythrocytic parasite for another cycle of red cell invasion\u0026nbsp;(Mohandas and An, 2013). Rigid RBCs contribute to disease pathology through sequestration of these cells and obstruction of the microvasculature, however its impact is primarily for the clearance of the parasitized cells in the spleen\u0026nbsp;(Deng \u003cem\u003eet al\u003c/em\u003e., 2015). The extract could have induced reduced cell deformability of the infected red cells thereby enhancing their host-mediated clearance in the inter-endothelial cleft of the spleen. This idea is supported by the fact that reduced deformability of infected red blood cell was reported to be a common feature antimalarial drug. Artemether, the standard drug in this study, is thought to act by a mechanism which generate reactive oxygen species that contribute to reduced iRBC deformability through lipid peroxidation\u0026nbsp;(Deng \u003cem\u003eet al\u003c/em\u003e., 2015). \u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eThe malaria parasite digest haemoglobin, the iron containing protein of the red blood cells, during its 48-hour intra-erythrocytic cycle to obtain amino acids, leading to depletion of haemoglobin and accumulation of free heme (Joof \u003cem\u003eet al\u003c/em\u003e., 2021). The result of this study showed that haemoglobin concentration in the untreated animals significantly (p \u0026lt; 0.05) reduced compared to the uninfected animals. The administration of the extract, on the other hand, significantly (p \u0026lt; 0.05) raised the haemoglobin concentration in the test groups. This result is consistent with prevous studies (Aragaw \u003cem\u003eet al.\u003c/em\u003e, 2020; Joshua \u003cem\u003eet al\u003c/em\u003e., 2020; Enechi \u003cem\u003eet al\u003c/em\u003e., 2021). The activity of some of the secondary metabolites in the extract could be responsible for the observed higher haemoglobin concentration in the treated animals. It could be adduced that some of the metabolites \u0026nbsp;inhibited the digestion of haemoglobin by the parasite. This is as some quinoline drugs such as chloroquine and amodiaquine have been found to inhibit haemoglobin degradation (Famin and Ginsgurg, 2002). The increased white blood cell count found in the untreated animals indicates the immune response of the animals to the plasmodium infection.\u0026nbsp;Reports have shown that severe malaria is characterized by leukocytosis. The extract however reduced the WBC count in the treated animals.\u0026nbsp;This confirms that the extract was able to clear the infecting Plasmodium parasite thereby reducing the stimulation of leukocytosis.\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eThe overall health of the liver is determined by a number of blood tests generally known as liver function tests. The tests evaluate the levels of several enzymes, proteins, and other chemicals produced by the liver. This test is employed in the assessment of liver damage, determine side effect of certain drugs, diagnosis of liver disease and monitoring of its treament. Alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), gammaglutamyl transferase, serum bilirubin, prothrombin time, and serum albumin are some of the commonly available liver function test. Several liver activities such as anion excretion (bilirubin), hepatocellular integrity (transaminases), bile production and subsequent free flow (bilirubin and ALP), and protein synthesis (albumin) are reflected by this test \u0026nbsp;(Hoekstra \u003cem\u003eet al\u003c/em\u003e., 2013; van Beek \u003cem\u003eet al\u003c/em\u003e., 2014). \u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eIn this study, the activities of serum liver enzymes namely AST, ALT and ALP were assayed, while the concentration of the serum bilirubin was determined to assess hepatocellular damage and impairment of liver function due to malaria parasite, as well as the effect of the ethanol extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e stem bark on the liver. The result indicated that there was significant lowering of in the activities of AST, ALT and ALP as well as the concentration of total bilirubin in all the test groups when compared to the untreated group. \u0026nbsp;The untreated group demonstrated an increase in all of the assessed metrics when compared to the normal control group. The increase in the activities of aminotransferases (AST, ALT) and alkaline phosphatase may be as a result of possible hepatocellular damage\u0026nbsp;(Gowda \u003cem\u003eet al\u003c/em\u003e., 2009)\u0026nbsp;while the increased hyperbilirubinemia could be due to parenchymal liver cell destruction and biliary blockage caused by malaria parasite, liver schizonts\u0026nbsp;(Limdi and Hyde, 2003; Hoekstra \u003cem\u003eet al\u003c/em\u003e., 2013). This finding is in agreement with earlier research\u0026nbsp;(Enechi \u003cem\u003eet al\u003c/em\u003e., 2021; Iyiola, 2020; Joshua \u003cem\u003eet al\u003c/em\u003e., 2020)\u0026nbsp;which discovered higher hepatic enzyme activity and hyperbilirubinemia in mice infected with \u003cem\u003ePlasmodium berghei\u003c/em\u003e. The reduction in increased AST, ALT, and ALP levels demonstrated that the extract was efficient in correcting plasmodium parasite-induced hepatocellular damage. It is evident from this result that the has hepatoprotective ability which could be due to the synergistic action of the several secondary metabolites in the extract. The hepatoprotective effect could also be due to the action of a single phytochemical in the extract.\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eThe concentration of bilirubin in plasma provides indirect information on the liver\u0026apos;s uptake, conjugation, and excretion functions. Elevated plasma concentration of bilirubin are specific markers for serious liver injury and, consequently, liver function loss\u0026nbsp;(Hoekstra \u003cem\u003eet al\u003c/em\u003e., 2013). The reduction in plasma concentration of bilirubin of mice infected with \u003cem\u003ePlasmodium berghei\u003c/em\u003e and treated with the extract shows that the extract reversed liver cell damage and restored liver cell function. This is congruent with the findings of Joshua \u003cem\u003eet al\u003c/em\u003e. (2020) which demonstrated that in mice with \u003cem\u003ePlasmodium berghei\u003c/em\u003e infection, \u003cem\u003eE. sengalensis\u003c/em\u003e extract reduced the total bilirubin levels.\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eVisser \u003cem\u003eet al\u003c/em\u003e., 2017 had reported changes in lipid profile associated with\u0026nbsp;malaria infection. This study determined the total cholesterol, HDL-cholesterol, LDL-cholesterol, and triglycerides. \u0026nbsp;The result of the showed that the ethanol extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e stem bark significantly (p \u0026lt; 0.05) reduced the total cholesterol, triglyceride and LDL cholesterol while significantly (p \u0026lt; 0.05) raising the HDL cholesterol in the treated animals compared to the untreated ones (positive control). Previous studies\u0026nbsp;(Enechi \u003cem\u003eet al\u003c/em\u003e., 2021; Joshua \u003cem\u003eet al\u003c/em\u003e., 2020), supports this result. This study further revealed that malaria infection results in mixed hyperlipoproteinemia in mice, which is characterized by increased total cholesterol, triglyceride, LDL-cholesterol and decreased HDL-cholesterol\u0026nbsp;(Natesan and Kim, 2021). However, the mechanism involved in the changes in plasma lipid profile related to malaria is yet to be completely understood. It has been suggested to be partly host-related\u0026nbsp;(Acharya and Patil, 2009). This is in contrast with the reportedly observed dyslipidemia in human malaria which showed increased triacylglycerol and decreased HDL, LDL and total cholesterol plasma concentrations in infected patients\u0026nbsp;( Visser \u003cem\u003eet al\u003c/em\u003e., 2013; Dias \u003cem\u003eet al\u003c/em\u003e., 2016; Sirak \u003cem\u003eet al\u003c/em\u003e., 2016; Visser \u003cem\u003eet al\u003c/em\u003e., 2017).\u0026nbsp;This observed difference could be due to differing pattern of lipid metabolism in mice and humans. The accumulation of triglyceride and LDL-cholesterol causes damage to the arteries, contributing to cardiovascular disease, while on the other hand HDL-cholesterol is useful in helping the body to get rid of bad cholesterol\u0026nbsp;(Natesan and Kim, 2021). The finding of this study reveal that the extract possesses anti-lipidemia activity as it was able to reduce total cholesterol, triglyceride and LDL-cholesterol in the treated mice. The anti-lipidemia effect of this extract as found in this study also supports the previous report of\u0026nbsp;Ogbonnia \u003cem\u003eet al\u003c/em\u003e. (2008), which demonstrated that the hydro ethanolic extract of \u003cem\u003eP. curatellifolia\u003c/em\u003e seed significantly reduced plasma lipid concentration in diabetic rats.\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eMalondialdehyde (MDA) is used as an indirect measure of the cumulative lipid peroxidation as it is a unstable end product of lipid peroxidation\u0026nbsp;(Mao \u003cem\u003eet al\u003c/em\u003e., 2019). This aldehyde known for its high cytotoxicity and carcinogenicity is a product of free radicals\u0026rsquo; attack on phospholipids. Increased level of MDA is associated with decreased antioxidant parameters\u0026nbsp;(Maurya \u003cem\u003eet al\u003c/em\u003e., 2021), and this is as a result of increased production of oxidative species\u0026nbsp;(Perc\u0026aacute;rio \u003cem\u003eet al\u003c/em\u003e., 2012), associated with malaria infection. The result of this study indicated that MDA concentration significantly (p \u0026lt; 0.05) reduced in the treated mice compared to the positive control (the untreated infected mice). This shows that the extract has free radical scavenging activity which could be attributed to the phytochemical constituents in the extract including flavonoids, phenols, tannins, and terpenoids. This finding is consistent with previous studies which showed\u0026nbsp;\u003cem\u003eParinari curatellifolia\u003c/em\u003e to be a potent scavenger of nitrite free radicals\u0026nbsp;(Boora \u003cem\u003eet al\u003c/em\u003e., 2014)\u0026nbsp;and inhibitor of phospholipid peroxidation due to free radical activity\u003cem\u003e\u0026nbsp;\u003c/em\u003e(Ndhlala \u003cem\u003eet al\u003c/em\u003e., 2006)\u003cem\u003e.\u0026nbsp;\u003c/em\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eSuperoxide dismutase (SOD) is utilized both the human host and \u003cem\u003ePlasmodium\u003c/em\u003e parasite as first line of defense against superoxide radicals. They\u0026nbsp;metalloproteins that dismutate superoxide radicals (O\u003csub\u003e2\u003c/sub\u003e\u003csup\u003e-\u003c/sup\u003e\u003csub\u003e\u0026nbsp;\u003c/sub\u003e) into hydrogen peroxide (H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e) and oxygen (O\u003csub\u003e2\u003c/sub\u003e) using copper/zinc (Cu/Zn), iron (Fe), or manganese (Mn) as cofactors\u0026nbsp;(Kavishe \u003cem\u003eet al\u003c/em\u003e., 2017). The SOD activity of the mice treated with the extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e stem bark was found to significantly (p \u0026lt; 0.05) increase compared to the untreated animals. The SOD activity of the untreated group decreased significantly compared to the uninfected group. This could be due to increased concentration of oxidants generated by the host cell in response to malaria infection, leading to the depletion of the enzyme and reduced activity observed. The extract however restored the activity of the enzyme to a level that is non-significantly different from the normal control in all the treated groups. This effect could be attributed to the antioxidant activity of the extract. The extract contains antioxidative phytochemicals such as flavonoids, phenols and tannins. Previous studies in rats\u0026nbsp;(Olaleye \u003cem\u003eet al\u003c/em\u003e., 2014;\u0026nbsp;Yakubu, 2012; Atawodi \u003cem\u003eet al\u003c/em\u003e., 2013), has\u0026nbsp;showed that \u003cem\u003eParinari curatellifolia\u003c/em\u003e possess antioxidant activity, is consistent with the result of our study.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eThe catalase activity of the treated animals in this study was also found to increase significantly (p \u0026lt; 0.05) in the group of mice treated with the extract compared to the untreated group. The catalase activity in the untreated group significantly decreased compared to the normal control, possibly due to excessive generation of hydrogen peroxide (H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e). Catalase limits the accumulation of H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e\u003csup\u003e\u0026nbsp;\u003c/sup\u003e(Kavishe \u003cem\u003eet al\u003c/em\u003e., 2017)\u003csup\u003e.\u003c/sup\u003e Previous studies has shown that malaria infection is associated with decreased activity of catalase\u0026nbsp;(Vasquez \u003cem\u003eet al\u003c/em\u003e., 2021). The result of this study antioxidative activity of the ethanol extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e stem bark. However, the result showed that the reduced glutathione (GSH) concentration significantly increased in the untreated group compared to the normal control (unparasitized mice). This observation is consistent with that of Stocker \u003cem\u003eet al\u003c/em\u003e., 1985, which reported increased GSH concentration in malaria patient with high number of infected red blood cells due to the enhancement of glutathione reductase. They suggested that significant portion of the increase was associated with the production of GSH by the parasite itself. The administration of the extract resulted in significant (p \u0026lt; 0.05) decrease in GSH concentration of the treated group compared to the untreated group. The effect might be due to generation of oxidative stress by a phytochemical component of the extract, as it is known that some anti-malarial drugs exert their antiplasmodial effect by increasing oxidative stress on the parasite. A study by\u0026nbsp;(Akanbi \u003cem\u003eet al\u003c/em\u003e., 2010) had reported lower levels of GSH in malarial patients treated with anti-malarial drug compared to patients who had not taken drug.\u003c/p\u003e"},{"header":"Conclusion","content":"\u003cp\u003eThe findings from this study so far have shown that the ethanol extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e stem bark can serve as an effective and safe regimen for the treatment of malaria in the mouse model. The extract is composed of phytochemicals that can be beneficial to human health, and caused no mortality in the acute toxicity test in mice, showing its safety for human use. The extract also effectively reduced parasitemia in infected mice, while restoring hematological aberrations including PCV, RBC count, WBC count, haemoglobin concentration and biochemical aberrations including liver enzymes activities, bilirubin concentration and lipid profile, induced by the \u003cem\u003ePlasmodium\u003c/em\u003e parasite. This study also showed that the extract countered lipid peroxidation by reducing the MDA level in treated animals as well as restored the antioxidant markers to the levels found in the uninfected animals. This study has so far demonstrated that ethanol extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e stem bark is effective and safe for the treatment of malaria, thereby justifying its use for managing malaria in various part of the African continent. This plant can as well serve as a source of lead compound for the development of a novel, safe and effective antimalarial drug if exploited.\u003c/p\u003e"},{"header":"Abbreviations","content":"\u003cp\u003eACT \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;Artemisinin combination therapy\u003c/p\u003e\n\u003cp\u003eALT\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;Alaninine aminotranferase\u003c/p\u003e\n\u003cp\u003eALP\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;Alkaline phosphatase\u003c/p\u003e\n\u003cp\u003eAST\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;Aspartate aminotransferase\u003c/p\u003e\n\u003cp\u003eCAT\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;Catalase\u003c/p\u003e\n\u003cp\u003eDCM-MeOH\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;Dichloromethame-methanol\u003c/p\u003e\n\u003cp\u003eEEPCSB\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;Ethanol extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e stem bark\u003c/p\u003e\n\u003cp\u003eFFA\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;Free fatty acids\u003c/p\u003e\n\u003cp\u003eFP\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;Ferriprotoporphyrin\u003c/p\u003e\n\u003cp\u003eGGT\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;Gamma-glutamyl transferase\u003c/p\u003e\n\u003cp\u003eGGTP\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;Gamma glutamyl transpeptidase\u003c/p\u003e\n\u003cp\u003eG6PD\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;Glucose-6-phosphate dehydrogenase\u003c/p\u003e\n\u003cp\u003eGSH\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;Reduced glutathione\u003c/p\u003e\n\u003cp\u003eHDL\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;High density lipoprotein\u003c/p\u003e\n\u003cp\u003eHgb\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;Haemoglobin\u003c/p\u003e\n\u003cp\u003eIC50\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;Median inhibitory concentration\u003c/p\u003e\n\u003cp\u003eLDL\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;Low density lipoprotein\u003c/p\u003e\n\u003cp\u003eMCV\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;Mean Cell Volume\u003c/p\u003e\n\u003cp\u003eMDA\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;Malondialdehyde\u003c/p\u003e\n\u003cp\u003ePCV\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;Packed cell volume\u003c/p\u003e\n\u003cp\u003eRBC\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;Red blood cell\u003c/p\u003e\n\u003cp\u003eRNS\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;Reactive nitrogen species\u003c/p\u003e\n\u003cp\u003eROS\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;Reactive oxygen species\u003c/p\u003e\n\u003cp\u003eSPSS\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;Statistical Product and Service Solution\u003c/p\u003e\n\u003cp\u003eSOD\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;Superoxide dismutase\u003c/p\u003e\n\u003cp\u003eVLDL\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;\u0026nbsp;Very low density lipoprotein\u003c/p\u003e\n\u003cp\u003eWBC\u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;White blood cell\u003c/p\u003e\n\u003cp\u003eWHO \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; World Health Organization\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eEthics approval\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThis study was conducted in line with the ethical rules and recommendations of the University of Nigeria Ethical committee on the care and use of laboratory animals.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConsent for publication\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eNot Applicable\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAvailability of data and materials\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe datasets used and analyzed during the current study are available from the corresponding author on reasonable request.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompeting interests\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe authors declare that they have no competing interest.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFunding\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThis research received no funding from any institution, organization or agency.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAuthors\u0026rsquo; contributions\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eLOM, PEJ, CCA; Conceptualization, Methodology, Software, LOM: data curation, writing \u0026ndash; original manuscript preparation, PEJ: supervision, PEJ, CCA, LOM: investigation, validation \u0026nbsp;PEJ, CCA, IO, JOF: writing \u0026ndash; reviewing and editing. All authors read and approve the final manuscript.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAcknowledgements\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eNot applicable.\u003c/p\u003e"},{"header":"References","content":"\u003cp\u003eAcharya M, Patil SL. Variation in common lipid parameters in malaria infected patients. \u0026nbsp;Indian J. Physiol. Pharmacol. 2009;53(3):271\u0026ndash;4.\u003c/p\u003e\n\u003cp\u003eAin QU, Khan H, Mubarak MS, Pervaiz A. Plant alkaloids as antiplatelet agent: Drugs of the future in the light of recent developments. Front. Pharmacol. 2016; doi.org/10.3389/fphar.2016.00292.\u003c/p\u003e\n\u003cp\u003eAjibola M, Oloruntoba AC, Yetusoko ND. Evaluation of Pcv and Hemoglobin Variations among Malaria Positive and Malaria Negative Patients , At the Ecwa Community Nenchi Dooshima Yetusoko. IOSR J.\u0026nbsp;Pharm. 2012;2(6):65\u0026ndash;69.\u003c/p\u003e\n\u003cp\u003eAkanbi OM, Odaibo AB, \u0026nbsp;Ademowo OG. 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International journal of molecular sciences. 2012;13(12):16346-72.\u003c/p\u003e\n\u003cp\u003ePrabhu SR, Ware AP, Saadi AV. Erythrocyte miRNA regulators and malarial pathophysiology. Infection, Genetics and Evolution. 2021;93:105000.\u003c/p\u003e\n\u003cp\u003eWorld Health Organization. World malaria report 2022. World Health Organization; 2022 Dec 8.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eRoy A. A review on the alkaloids an important therapeutic compound from plants Remediation of environmental contamination View project Micropropagation of Centella asiatica View project. International Journal of Plant Biotechnology. 2017;\u003cem\u003e3\u003c/em\u003e(2):1\u0026ndash;9.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eSaxena S, Pant N, Jain DC, \u0026nbsp;Bhakuni RS. Antimalarial agents from plant sources. 2003;\u003cem\u003e85\u003c/em\u003e(9):1314\u0026ndash;1329.\u003c/p\u003e\n\u003cp\u003eSirak S, Fola AA., Worku L, \u0026nbsp;Biadgo B. Malaria parasitemia and its association with lipid and hematological parameters among malaria- infected patients attending at Metema Hospital , Northwest Ethiopia. Pathology and Laboratory Medicine International. 2016;\u003cem\u003e8\u003c/em\u003e: 43\u0026ndash;50.\u003c/p\u003e\n\u003cp\u003eSylvanus U, Olakunle F, Amos J, Olutayo O. Antibacterial activity and phytochemical evaluation of the leaf root and stem bark extracts of parinari curatellifolia (planch. ex benth). Int J Adv Chem. 2014;2:178-81.\u003c/p\u003e\n\u003cp\u003eTajbakhsh E, Kwenti TE, Kheyri P, Nezaratizade S, Lindsay DS, \u0026nbsp;Khamesipour F. Antiplasmodial, antimalarial activities and toxicity of African medicinal plants: a systematic review of literature. \u003cem\u003eMalaria Journal\u003c/em\u003e, 2021;\u003cem\u003e20\u003c/em\u003e(1). doi.org/10.1186/s12936-021-03866-0\u003c/p\u003e\n\u003cp\u003eTong Z, He W, Fan X, Guo A. Biological function of plant tannin and its application in animal health. Frontiers in Veterinary Science. 2022;8:1597.\u003c/p\u003e\n\u003cp\u003eTungmunnithum D. Thongboonyou A, Pholboon A, \u0026nbsp;Yangsabai A. Flavonoids and Other Phenolic Compounds from Medicinal Plants for Pharmaceutical and Medical Aspects: An Overview.\u0026nbsp;Medicines 2018;5(3), 93.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eUngogo MA, Ebiloma GU, Ichoron N, Igoli JO, de Koning HP, \u0026nbsp;Balogun EO.\u0026nbsp;A Review of the Antimalarial, Antitrypanosomal, and Antileishmanial Activities of Natural Compounds Isolated From Nigerian Flora. \u003cem\u003eFrontiers in Chemistry\u003c/em\u003e. 2020; doi.org/10.3389/fchem.2020.617448\u003c/p\u003e\n\u003cp\u003evan Beek JH, de Moor MH, de Geus EJ, Lubke GH, Vink JM, Willemsen G, Boomsma DI. The genetic architecture of liver enzyme levels: GGT, ALT and AST. Behavior genetics. 2013;43:329-39.\u003c/p\u003e\n\u003cp\u003eVasquez M, Zuniga M, Rodriguez A. Oxidative Stress and Pathogenesis in Malaria. Front\u003cem\u003e\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/em\u003eCell Infect Microbiol. 2021; doi.org/10.3389/fcimb.2021.768182\u003c/p\u003e\n\u003cp\u003eVenugopal K, Hentzschel F, Valkiūnas G, Marti M. Plasmodium asexual growth and sexual development in the haematopoietic niche of the host. Nature Reviews Microbiology. 2020;18(3):177-89.\u003c/p\u003e\n\u003cp\u003eVisser BJ, de Vries SG, Vingerling R, Gritter M, Kroon D, Aguilar LC, Kraan RB, Wieten RW, Danion F, Sjouke B, Adegnika AA. Serum lipids and lipoproteins during uncomplicated malaria: a cohort study in Lambarene, Gabon. The American Journal of Tropical Medicine and Hygiene. 2017;96(5):1205.\u003c/p\u003e\n\u003cp\u003eVisser BJ, Wieten RW, Nagel IM, Grobusch MP. Serum lipids and lipoproteins in malaria-a systematic review and meta-analysis. Malaria journal. 2013;12(1):1-6.\u003c/p\u003e\n\u003cp\u003eYakubu OE, Atawodi SE, Ojogbane E, Nwaneri-Chidozie VO. Acute toxicity and antioxidant activity of Parinari curatellifollia root methanolic extract in carbon tetrachloride-induced toxicity in wistar rats. Inter J Basic Appl Chem Sci. 2012;2(3):82-9.\u003c/p\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Parinari curatellifolia, antimalarial, parasitemia, Plasmodium berghei, ethanol extract, Mobola plum","lastPublishedDoi":"10.21203/rs.3.rs-3586444/v2","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-3586444/v2","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cstrong\u003eBackground:\u003c/strong\u003e The use of \u003cem\u003eParinari curatellifolia\u003c/em\u003e for the management of malaria by Africans without scientific validation, prompted this study, to investigate the antimalarial effect of ethanol extract of \u003cem\u003eParinari curatellifolia\u003c/em\u003e stem bark (EEPCSB) in \u003cem\u003ePlasmodium berghei (NK-65)\u003c/em\u003e-infected mice.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethod:\u003c/strong\u003e Thirty mice (16 -21 g) were grouped into six of five mice each. Group 1 (uninfected) served as normal control while groups 2-6 were infected\u003cem\u003e.\u003c/em\u003e Group 2 (positive control) was untreated while group 3 (standard control) received 20/120 mg/kg b.w. of arthemeter/lumefantrine, groups 4-6 received 200, 400 and 600 mg/kg b.w. of EEPCSB respectively. The treatment lasted for four consecutive days. Parasitemia, heamatological and biochemical tests were carried out using established methods. The obtained data were analysed using one-way analysis of variance (ANOVA).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eResult: \u003c/strong\u003eThe extract significantly (p \u0026lt; 0.05) suppressed the parasitemia, and produced significant (p \u0026lt; 0.05) increase in the PCV, RBC, and Hgb and a significant (p \u0026lt; 0.05) decrease in the WBC of groups 4-6 compared to the positive control. The liver function marker enzymes - AST, ALT, ALP, and total bilirubin were all significantly (p \u0026lt; 0.05) reduced in the treated animals, while there was a significant (p \u0026lt; 0.05) decrease in the total cholesterol, triacylglycerol and LDL, and a significant (p \u0026lt; 0.05) increase in the HDL of the infected animals compared to the positive control. While there was a significant (p \u0026lt; 0.05) reduction in lipid peroxidation marker MDA in the treated groups, the activities of the SOD and catalase, significantly (p \u0026lt; 0.05) increased compared to the positive control. The GSH concentration reduced significantly (p \u0026lt; 0.05) in the treated groups compared to the untreated group.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConclusion:\u003c/strong\u003e The extract showed excellent antimalarial effect by reducing parasitemia and reversing haematological and biochemical aberrations in \u003cem\u003ePlasmodium berghei\u003c/em\u003e-infected mice as demonstrated in this study. Therefore, \u003cem\u003eParinari curatellifolia\u003c/em\u003e stem bark could be a source of lead compound for a novel and effective antimalarial drug.\u003c/p\u003e","manuscriptTitle":"Antimalarial effect of ethanol extract of Parinari curatellifolia stem-bark in Plasmodium berghei-NK 65 infected mice.","msid":"","msnumber":"","nonDraftVersions":[{"code":2,"date":"2024-04-16 21:46:38","doi":"10.21203/rs.3.rs-3586444/v2","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}},{"code":1,"date":"2023-11-13 07:12:11","doi":"10.21203/rs.3.rs-3586444/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"c8f30564-e9c8-4ab2-964b-32318ded16ef","owner":[],"postedDate":"April 16th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2023-11-17T18:30:52+00:00","versionOfRecord":[],"versionCreatedAt":"2024-04-16 21:46:38","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v2","identity":"rs-3586444","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-3586444","identity":"rs-3586444","version":["v2"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}