Ganoderic Acid A enhances glycometabolism in mouse gastrocnemius muscle during exercise-induced fatigue via AMPK/PGC-1α pathway activation

Ganoderic Acid A enhances glycometabolism in mouse gastrocnemius muscle during exercise-induced fatigue via AMPK/PGC-1α pathway activation | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 19 April 2025 V1 Latest version Share on Ganoderic Acid A enhances glycometabolism in mouse gastrocnemius muscle during exercise-induced fatigue via AMPK/PGC-1α pathway activation Authors : Jialin Zhu , Fenglin Peng , Lin Zhang , Yanju Guo , Weiguo Liu , Jingrong Li , Shuju Shang , Bohan Zhang , and Taotao Qiu 0000-0001-8659-5364 [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.174504963.33711366/v1 Published Food Science & Nutrition Version of record Peer review timeline 216 views 116 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Objective: Exercise-induced fatigue (EIF) is closely associated with impaired glycometabolism in skeletal muscle. This study investigated the protective effects of Ganoderic acid A (GAA) on glycometabolism in EIF mice and explored its underlying mechanisms. Methods: Sixty KM mice were divided into five groups: a blank control (BC), a model control (MC), and three GAA-treated groups (20, 40, and 60 mg/kg/d). After a 7-week intervention, exhaustive treadmill tests and biochemical analyses were conducted to assess fatigue resistance, metabolic parameters, and molecular pathways. Results: GAA administration significantly prolonged the exhaustive running time (p < 0.01), reduced serum levels of blood urea nitrogen (BUN), creatine kinase (CK), lactate dehydrogenase (LDH), and lactate (LD) (p < 0.05), and increased glycogen content in the liver and gastrocnemius muscle. Mechanistically, GAA activated AMPK phosphorylation, upregulated PGC-1α and GLUT4 expression, and enhanced succinate dehydrogenase (SDH) and Ca²⁺-Mg²⁺-ATPase activities. Conclusion: The results demonstrate that GAA alleviates EIF by enhancing energy metabolism through the AMPK/PGC-1α/GLUT4 pathway. These findings highlight GAA as a promising natural supplement for combating exercise-induced fatigue by glycometabolic. Supplementary Material File (manuscript.pdf) Download 1.62 MB Information & Authors Information Version history V1 Version 1 19 April 2025 Peer review timeline Published Food Science & Nutrition Version of Record 6 Mar 2026 Published Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords endocrine pharmacology natural product pharmacodynamics Authors Affiliations Jialin Zhu Guangxi Normal University View all articles by this author Fenglin Peng Guangxi Normal University View all articles by this author Lin Zhang Guangxi Normal University View all articles by this author Yanju Guo Guangxi Normal University View all articles by this author Weiguo Liu Guangxi Normal University View all articles by this author Jingrong Li Guangxi Normal University View all articles by this author Shuju Shang Guangxi Normal University View all articles by this author Bohan Zhang Guangxi Normal University View all articles by this author Taotao Qiu 0000-0001-8659-5364 [email protected] Guangxi Normal University View all articles by this author Metrics & Citations Metrics Article Usage 216 views 116 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Jialin Zhu, Fenglin Peng, Lin Zhang, et al. Ganoderic Acid A enhances glycometabolism in mouse gastrocnemius muscle during exercise-induced fatigue via AMPK/PGC-1α pathway activation. Authorea . 19 April 2025. DOI: https://doi.org/10.22541/au.174504963.33711366/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . Format Please select one from the list RIS (ProCite, Reference Manager) EndNote BibTex Medlars RefWorks Direct import Tips for downloading citations document.getElementById('citMgrHelpLink').addEventListener('click', function() { popupHelp(this.href); return false; }); $(".js__slcInclude").on("change", function(e){ if ($(this).val() == 'refworks') $('#direct').prop("checked", false); $('#direct').prop("disabled", ($(this).val() == 'refworks')); }); View Options View options PDF View PDF Figures Tables Media Share Share Share article link Copy Link Copied! Copying failed. Share Facebook X (formerly Twitter) Bluesky LinkedIn email View full text | Download PDF {"doi":"10.22541/au.174504963.33711366/v1","type":"Article"} Now Reading: Share Figures Tables Close figure viewer Back to article Figure title goes here Change zoom level Go to figure location within the article Download figure Toggle share panel Toggle share panel Share Toggle information panel Toggle information panel Go to previous graphic Go to next graphic Go to previous table Go to next table All figures All tables View all material View all material xrefBack.goTo xrefBack.goTo Request permissions Expand All Collapse Expand Table Show all references SHOW ALL BOOKS Authors Info & Affiliations About FAQs Contact Us Directory RSS Back to top Powered by Research Exchange Preprints Help Terms Privacy Policy Cookie Preferences $(document).ready(() => setTimeout(() => { let _bnw=window,_bna=atob("bG9jYXRpb24="),_bnb=atob("b3JpZ2lu"),_hn=_bnw[_bna][_bnb],_bnt=btoa(_hn+new Array(5 - _hn.length % 4).join(" ")); $.get("/resource/lodash?t="+_bnt); },4000)); (function(){function c(){var b=a.contentDocument||a.contentWindow.document;if(b){var d=b.createElement('script');d.innerHTML="window.__CF$cv$params={r:'a002b0c7f8f2df94',t:'MTc3OTUyNDk0Mg=='};var a=document.createElement('script');a.src='/cdn-cgi/challenge-platform/scripts/jsd/main.js';document.getElementsByTagName('head')[0].appendChild(a);";b.getElementsByTagName('head')[0].appendChild(d)}}if(document.body){var a=document.createElement('iframe');a.height=1;a.width=1;a.style.position='absolute';a.style.top=0;a.style.left=0;a.style.border='none';a.style.visibility='hidden';document.body.appendChild(a);if('loading'!==document.readyState)c();else if(window.addEventListener)document.addEventListener('DOMContentLoaded',c);else{var e=document.onreadystatechange||function(){};document.onreadystatechange=function(b){e(b);'loading'!==document.readyState&&(document.onreadystatechange=e,c())}}}})();