Exploring correlation between alkaline phosphatase and venous thromboembolism in East Asian populations: a cohort and Mendelian randomization study

Exploring correlation between alkaline phosphatase and venous thromboembolism in East Asian populations: a cohort and Mendelian randomization study | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Exploring correlation between alkaline phosphatase and venous thromboembolism in East Asian populations: a cohort and Mendelian randomization study Haichao Wu, Haobo Li, Tao Wang, Zhongtiao Xu, Haiwei Chu, Long Zhou, and 4 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6431721/v2 This work is licensed under a CC BY 4.0 License Status: Posted Version 2 posted You are reading this latest preprint version Show more versions Abstract Background Several studies have explored the association between alkaline phosphatase (ALP) and venous thromboembolism (VTE), yet the findings remain inconclusive and inconsistent. The correlation between them has yet to be definitively established. We conducted a multicenter cohort study and a two-sample MR analysis with the aim of investigating the association between serum ALP levels and acute VTE in East Asian populations. Methods We collected data on VTE patients from Taizhou Municipal Hospital and Taizhou Hospital between January 2019 and October 2024, alongside data from routine health check-up participants as the control group. Employing propensity score matching (PSM) and cubic spline model analysis, we investigated the relationship between ALP levels and VTE. Additionally, we performed a bidirectional two-sample Mendelian randomization (MR) analysis using GWAS data to assess the potential causal effect between them. Results The retrospective cohort study involving 720 VTE patients and 1,000 healthy controls found significantly lower ALP levels in the VTE group, which remained consistent after propensity score matching, with a non-linear relationship between ALP levels and VTE risk. Two-sample MR analysis confirmed a negative causal effect of ALP on VTE, with no evidence of pleiotropy in the results. Conclusions Our study suggested a negative association between ALP levels and the risk of VTE in the East Asian population. This finding may provide valuable insights into the role of ALP as a potential biomarker for VTE risk assessment. alkaline phosphatase venous thromboembolism cohort study Mendelian randomization Full Text Additional Declarations The authors declare no competing interests. Supplementary Files SupplementaryFigure1.Scatterplot.png SupplementaryFigure2.funnelplots.png SupplementaryFigure3.leaveoneout.png SupplementaryTable1.csv SupplementaryTable2.csv SupplementaryTable3.docx Cite Share Download PDF Status: Posted Version 2 posted You are reading this latest preprint version Show more versions Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. 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randomization","lastPublishedDoi":"10.21203/rs.3.rs-6431721/v2","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-6431721/v2","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003ch2\u003eBackground\u003c/h2\u003e \u003cp\u003eSeveral studies have explored the association between alkaline phosphatase (ALP) and venous thromboembolism (VTE), yet the findings remain inconclusive and inconsistent. The correlation between them has yet to be definitively established. We conducted a multicenter cohort study and a two-sample MR analysis with the aim of investigating the association between serum ALP levels and acute VTE in East Asian populations.\u003c/p\u003e\u003ch2\u003eMethods\u003c/h2\u003e \u003cp\u003eWe collected data on VTE patients from Taizhou Municipal Hospital and Taizhou Hospital between January 2019 and October 2024, alongside data from routine health check-up participants as the control group. Employing propensity score matching (PSM) and cubic spline model analysis, we investigated the relationship between ALP levels and VTE. Additionally, we performed a bidirectional two-sample Mendelian randomization (MR) analysis using GWAS data to assess the potential causal effect between them.\u003c/p\u003e\u003ch2\u003eResults\u003c/h2\u003e \u003cp\u003eThe retrospective cohort study involving 720 VTE patients and 1,000 healthy controls found significantly lower ALP levels in the VTE group, which remained consistent after propensity score matching, with a non-linear relationship between ALP levels and VTE risk. Two-sample MR analysis confirmed a negative causal effect of ALP on VTE, with no evidence of pleiotropy in the results.\u003c/p\u003e\u003ch2\u003eConclusions\u003c/h2\u003e \u003cp\u003eOur study suggested a negative association between ALP levels and the risk of VTE in the East Asian population. This finding may provide valuable insights into the role of ALP as a potential biomarker for VTE risk assessment.\u003c/p\u003e","manuscriptTitle":"Exploring correlation between alkaline phosphatase and venous thromboembolism in East Asian populations: a cohort and Mendelian randomization study","msid":"","msnumber":"","nonDraftVersions":[{"code":2,"date":"2025-09-03 12:57:05","doi":"10.21203/rs.3.rs-6431721/v2","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}},{"code":1,"date":"2025-05-07 06:08:35","doi":"10.21203/rs.3.rs-6431721/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"0d56f2f2-fda7-49ed-aff5-f3127ff6b6b9","owner":[],"postedDate":"September 3rd, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2025-05-16T11:38:48+00:00","versionOfRecord":[],"versionCreatedAt":"2025-09-03 12:57:05","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v2","identity":"rs-6431721","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-6431721","identity":"rs-6431721","version":["v2"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}