Inhibition effect of an antiangiogenesis therapy on endometrium growth in a nude mouse model

Objective:To investigate the effect of an antiangiogenesis therapy on inhibition of endometrium growth in the nude mouse model.Methods:Recombinant adenovirus was constructed with AdEasy-1 system and AAV 293 cells.Apoptosis of ECV-304 cells was induced by Ad-ES and growth inhibition was observed.The nude mouse endometriosis model was established by subcutaneous implantation.Injected in local focus with Ad-ES,Ad-Track or physiologic saline,the ectopic focuses were detected by microscopy after HE staining.The microvessel densities (MVD) were detected by immunohistochemistry and the apoptosis were detected by TUNEL.Results:The conduction of Ad-ES was proved by PCR,and titer of Ad-ES was 2.06×1010 pfu /ml.The apoptosis of ECV-304 cells induced by Ad-ES was analyzed by flow cytometer and Hoechst 33258 staining.The nude mouse endometriosis model was established by subcutaneous implantation,and the volume of endometriotic lesions treated by Ad-ES was deminished significantly compared with the other two control groups (P 0.05).MVD and the apoptosis were decreased significantly in the group using AdES compared with the two control groups.Conclusion:ES can induce ECV-304 cells to apoptosis and can inhibit the growth of endometrium in the nude mouse model.Antiangiogenesis may be a potential way for therapy of endometriosis.