Design, synthesis and bioactivity studies of flavonol bio-containing β-amino alcohols

Design, synthesis and bioactivity studies of flavonol bio-containing β-amino alcohols | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Design, synthesis and bioactivity studies of flavonol bio-containing β -amino alcohols Yujiao Qiu, Chunmei Hu, Qing Zhou, Qingxue Hu, Dan Shen, Xianghui Ruan, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6989432/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract A series of flavonoid derivatives containing β -amino alcohols were designed and synthesized using the natural product flavonols as the lead compounds, and their structures were verified by NMR and HRMS, and the antimicrobial activities of 21 flavonoid derivatives containing β -amino alcohols were systematically evaluated against nine plant pathogenic bacteria. Among the tested compounds, some of them showed good inhibitory effects against Phytophthora capsici ( P.c ), Sclerotinia mycoides ( S.s ), and Phytophthora kiwifruit anopheles ( P.s ). It is noteworthy that compound Y17 exhibited significant inhibitory activity against P.c . At a treatment concentration of 100 µg/mL, Y17 inhibited mycelial growth by 100%, and its half effective concentration (EC 50 ) was 5.2 µg/mL, which was significantly better than that of the azoxystrobin ( Az , 98.4 µg/mL). In addition, at a concentration of 200 µg/mL, the protective (85.7%) and curative (77.8%) effects of Y17 against eggplant fruit blight were significantly higher than those of the Az (42.9% protective and 35.5% curative effects). Scanning electron microscopy (SEM) further confirmed the disruption of bacterial membrane integrity by Y17 . The kinetic simulations showed that Y17 has similarity in nature and energy with the commercial drug Az . Therefore, flavonols derivatives containing β -amino alcohols have a high probability to be used as potential antifungal agents. flavonols β-amino alcohols phytophthora capsica (P.c) mechanism of fungal activity Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Figure 9 Figure 10 Figure 11 Figure 12 1 Introduction Plant diseases are a major factor constraining agricultural production and development, significantly reducing crop yield and quality, and causing severe economic losses [ 1 , 2 ]. The primary pathogenic agents of plant diseases include microorganisms such as fungi [ 3 ], bacteria [ 4 ], and viruses [ 5 , 6 ]. Among them, P.c , a highly destructive plant pathogenic oomycete [ 7 ], can infect a wide range of economically important crops, including Solanaceae (capsicum annuum, solanum lycopersicum [ 8 ], solanum tuberosum, solanum melongena) and Cucurbitaceae [ 9 ] (Cucumis sativus, Cucurbita moschata, Citrullus lanatus, Cucumis melo, Cucurbita pepo) species. Currently, chemical fungicides remain the primary means of controlling plant diseases [ 10 ]. However, the long-term and exclusive use of traditional fungicides can easily lead to pathogen resistance, increasing the difficulty of disease management [ 11 ]. Therefore, the development of low-toxicity, highly effective, and environmentally friendly novel pesticides has become a key research focus in the field of plant protection. Natural products, also known as secondary metabolites, encompass a majority of chemical substances such as flavonoids, phenols, terpenoids, alkaloids, terpenes, and polysaccharides [ 12 , 13 ]. Among these, flavonol compounds are widely distributed in various plants [ 14 ] (ginkgo, onion, tea). Studies have shown that flavonoids have bacteriostatic activity [ 15 , 16 ], and this class of compounds exhibits varying degrees of inhibitory effects on a wide range of microorganisms, including gram-positive, gram-negative [ 17 ], and fungi [ 18 ]. Due to their remarkable antimicrobial efficacy, flavonol compounds hold significant research value and application potential. β -Amino alcohols are important fundamental chemical raw materials [ 19 ] with extensive applications across multiple fields, primarily including organic synthetic chemistry [ 20 ], pharmaceutical chemistry [ 21 ], natural product synthesis, and chemical production [ 22 ]. Moreover, β -amino alcohols exhibit wide-ranging applications in medicine [ 23 ], grinding aids [ 24 ], plasticizers, and surfactant. Many β -amino alcohol derivatives have attracted considerable attention due to their remarkable biological activities, including antiviral [ 25 ], antibacterial [ 26 ], antioxidant, anti-inflammatory, antiproliferative, and anticancer properties [ 27 , 28 ]. Based on the natural product flavonol as a lead compound, we designed and synthesized a series of flavonol derivatives containing β -amino alcohol motifs through a scaffold-hopping strategy (Fig. 1 ). The antifungal activity of all 21 synthesized compounds was evaluated against nine fungal strains. Y17 exhibited the most potent inhibitory effect against P.c , prompting further investigation into its antifungal mechanism of action. Figure 1 . 2 Results and Discussion 2.1 Chemistry The target compounds Y1-Y21 were synthesized via a four-step reaction sequence, with the synthetic route illustrated in Scheme 1 . The structure of Y1 - Y21 was characterized using NMR and HRMS. Detailed physical characteristics and spectral data are provided in the supporting information. Scheme 1 2.2 Analysis of antifungal activity 2.2.1 Antifungal activity analysis in vitro The antibacterial activity of Y1 - Y21 against 9 plant fungi was measured at 100 µg/mL. From Table 1 , the experimental results demonstrated that several compounds exhibited significant antifungal activity at a concentration of 100 µg/mL. Notably, compounds Y11 , Y12 , Y15 , Y16 , Y17 , Y18 , Y21 exhibited > 90% inhibition rates against P.c outperforming Az (48.9%), compounds Y11 , Y12 , Y16 , Y17 , Y19 and Y20 exhibited > 90% inhibition rates against S.s , outperforming Az (88.8%), compounds Y12 , Y17 , Y18 , Y19 and Y20 exhibited > 90% inhibition rates against P.s , outperforming Az (60.6%), the inhibition rates of Y15 , Y17 , and Y21 on Botryosphaeria dothidea (B.d) were 75.9, 82.4 and 74.3%, which were superior than Az (69.1%), the inhibition rates of Y20 on Fusarium graminearum (F.g) were 74.4%, which were superior than Az (72.7%), To further investigate the antifungal potential of these compounds, the EC 50 values of the most active derivatives were determined according to published methodologies, with detailed results presented in Table 2 . According to Table 2 , the EC 50 of compounds Y17 and Y21 against P.c were 5.2, 7.6 µg/mL, respectively, which were superior to that of Az (98.5 µg/mL), and those of Y12 and Y20 against S.s were 13.4 and 10.5 µg/mL, respectively, which were superior to that of Az (50.1 µg/mL). Among them, Y17 had the best EC 50 value, and the antifungal effect was the most significant. the growth of P.c at different concentrations of Y17 (0, 3.125, 6.25, 25, and 50 µg/mL) and Az (0, 50, 100, 150, and 200 µg/mL) is shown in Fig. 2 . Table 1 . Table 2 . Figure 2 . 2.2.2 Structure − activity relationship analysis. To further investigate the effect of R 1 , R 2 and R 3 substituents on the inhibitory activity of the compounds, their structure-activity relationships were synthesized. As shown in Table 1 , Y1, Y2, Y3, Y4, and Y6 all showed less than 30% inhibition of P.c , we can speculate that when R 3 is the same, changes in the substituents R 1 and R 2 of the compounds do not have much effect on the fungicidal activity. However, after replacing R 3 from unsaturated to saturated hydrocarbon group, the inhibitory activity of the compounds is significantly enhanced. Among them, Y11 (96.7), Y12 (90.5), Y15 (93.5), Y16 (92.6), Y17 (100), Y18 (97.1), Y19 (91.3) and Y21 (92.6%) showed more than 90% of fungicidal activity. Notably, although the fungicidal activity of Y5 (28.1%) and Y13 (53.7%) with the introduction of short-chain saturated hydrocarbon groups was still lower than 60%, the overall experimental data indicated that the introduction of saturated hydrocarbon groups could improve the fungicidal activity of the compounds. 2.3 Antifungal activity of Y17 against P.c in vivo In this experiment, Y17 was selected for in vivo antifungal activity assay on eggplant fruits. As shown in Table 3 and Fig. 3 the range of eggplant fruit spots affected by Y17 was significantly smaller than that of the control agent Az , indicating that Y17 had good protective and curative effects. At a concentration of 200 µg/mL, Y17 had strong protective and curative effects on eggplant (85.7 and 77.8%, respectively), which was superior to Az (42.9 and 35.5%). The curative and protective effects of Y17 on eggplant at a concentration of 100 µg/mL were also stronger than those of the control agent. The results showed that Y17 had good antifungal activity on eggplant fruits in vivo and could effectively control fungal diseases of eggplant crops. Table 3 . Figure 3 . 2.4 SEM mycelial morphology analysis The effect of Y17 on the mycelial morphology of P.c was further investigated using SEM, and the results were consistent with the conclusions of the pre-fungal inhibitory activity test, which showed that the mycelial morphology of mycosphaerella treated with Y17 was significantly changed. As shown in Fig. 4 , the mycelium of the undrug-treated control group had a full morphology with a smooth and uniform surface. When the concentration of Y17 was 12.5 µg/mL, the mycelium produced folds, a few mycelia were bent, and growth was inhibited. When the concentration of Y17 was increased to 25 µg/mL, most of the mycelium surface appeared serious shrinkage, wrinkles, mycelium became flattened and bent, and the growth status was obviously poor. In summary, Y17 could destroy the mycelial morphology of P.c mycelium and inhibit its normal growth. And the degree of mycelium destruction was intensifying as the concentration increased. Figure 4 . 2.5 Fluorescence microscope (FM) experiments Propidium iodide (PI) is a nuclear DNA stain that does not pass through living cell membranes, but can cross broken cell membranes to stain the nucleus, and is commonly used for apoptosis detection. As shown in Fig. 5 , after different concentrations of Y17 (100 and 50 µg/mL) treated mycelium and stained with PI, the morphology of mycelium as well as the fluorescence effect was observed under fluorescence electron microscope, the results showed that the morphology of undrugged mycelium was intact and full, and there was no red fluorescence after staining, and the mycelium was destroyed and the red fluorescence became more and more obvious with the increasing concentration of Y17 , which indicated that the destruction of the cell membrane of P.c cells by the elevated concentration of the drug was intensifying and causing cell death. Figure 5 2.6 ROS accumulation ROS is a by-product of biological aerobic metabolism, when the level of ROS increases dramatically, it will cause an imbalance of the body's oxidation-antioxidant action, oxidative stress, causing lipid peroxidation, and ultimately lead to cell death. Among them, DCFH-DA fluorescent probe itself has no fluorescence, but it can freely pass through the cell membrane, and once it enters the cell, it can be hydrolyzed by intracellular esterase to generate non-fluorescent 2,7-dichlorodihydrofluorescein (DCFH), which is a fluorescence-less fluorescence. Once inside the cell, it can be hydrolyzed by intracellular esterase to form DCFH, and then DCFH will be oxidized by intracellular ROS to form a strong fluorescent product 2,7-dichlorofluorescein (DCF). Therefore, the level of ROS in the mycelial cells of P.c was determined by using DCFH-DA as an indicator of oxidative stress [ 28 ]. As show in Fig. 6 , the blank control group showed almost no green fluorescence, whereas the fluorescence intensity of P.c mycelium treated with different concentrations of Y17 increased with the concentration of Y17 , further indicating that Y17 can induce ROS production and cause membrane lipid peroxidation oxidation. Figure 6 . 2.7 Effect on cytoplasmic leakage Cells contain many macromolecules, and rupture of the cell membrane releases proteins, nucleic acids, and other macromolecules. The macromolecular leakage content of cell contents can be determined by measuring the absorbance of mycelial suspensions at 260 and 280 nm. From Fig. 8 , it can be clearly seen that the absorbance value of the mycelial suspension treated with Y17 increased significantly compared with the control group, Y17 and with the increase in the concentration of Y17 solution absorbance also increased, which shows that the Y17 treatment can increase the amount of leakage of cytoplasmic contents, causing cell membrane damage, and the degree of damage increases with the increase in concentration. Figure 7 . 2.8 Exploration of the determination of cell membrane permeability The cell membrane serves as an important barrier for communication between the cell and the external environment, providing a relatively stable internal environment for the cell's life activities, and plays an important role in the normal life activities of the cell. When the cell membrane is damaged, intracellular electrolytes will extravasate and the relative conductivity will become larger. The relative conductivity changes of mycelium treated with different concentrations of Y17 (0, 12.5, 25, 50, 100 µg/mL) are shown in Fig. 9 . The results showed that, after 1 h of dosing, the relative conductivity value of Y17 concentration of 100 µg/mL (85.7%) was 3.2 times higher than that of the blank group (26.3%), and the relative conductivity value of Y17 concentration of 12.5 µg/mL (62.6%) was 2.3 times higher than that of the blank group (26.3%), and the relative conductivity became larger with the increase of Y17 concentration. When the action time of Y17 was between 1–3 hours, the conductivity showed a more obvious upward trend and increased proportionally with the drug concentration and action time. These results suggest that Y17 can destroy mycelial cell membranes and increase cell membrane permeability, thus leading to fungal death. Figure 8 . 2.9 Cell dry weight experiment Mycelial dry weight measurements were used to assess mycelial growth, and the experimental results were consistent with previous in vitro antifungal activity tests. As shown in Fig. 10 , the dry weight of mycelium gradually decreased and the antifungal activity was enhanced as the concentration of Y17 increased. The concentration of Y17 was directly proportional to the antifungal activity and inversely proportional to the dry weight of mycelium. It can be seen that Y17 can inhibit the normal growth of mycelium. Figure 9 . 2.10 Determination of the MDA content MDA is one of the end products of lipid peroxidation and is a major indicator of monitoring cell membrane permeability, and its production can exacerbate cell membrane damage. Malondialdehyde yield was measured using malondialdehyde kit to detect the effect of Y17 on cell membrane, and the results were shown in Fig. 11 , which showed that the malondialdehyde content of Y17 concentration of 100, 50, 25, and 12.5 µg/mL were increased by 1.88, 1.72, 1.44, and 1.37 times, respectively, in a dose-dependent manner, compared with that of the control group. The above results indicated that the drug could disrupt the cell membrane of P.c leading to cell membrane damage. Figure 10 . 2.11 Safety analysis As shown in Fig. 12 , spraying Y17 at concentrations of 100, 200 and 400 µg/mL and equal doses of DMSO on chili pepper seeds was found to have almost no effect on the germination of chili pepper seeds. Similarly, spraying concentrations of 100, 200 and 400 µg/mL of Y17 and equal doses of DMSO on chili peppers with the same growth cycle had almost no effect on the growth of chili peppers. It indicates that Y17 has no significant effect on the germination and growth of chili pepper seeds and has good biosafety. Figure 11 . 2.12 Energy calculation From the Table 4 , it can be concluded that Az and Y17 have similar molecular weights and number of benzene rings, and both have similar core aromatic skeletons, which gives them similar membrane permeability and implies that they have similar solubility in solvents. E total: Az (-1389.0443 Eh) is comparable to Y1 7 (-1327.1366 Eh), indicating that they have similar stability. Az and Y17 differed in electronic structure and reactivity. Activation energy ( Ea ): the energy barrier of Az (0.1728 eV) is higher than that of Y17 (0.1448 eV), which indicates that Y17 is more reactive and kinetically more susceptible to reactions. The HOMO-LUMO energy gap (Δ E ): the energy barrier of Az (0.1728 eV) is greater than that of Y17 (0.1448 eV), which means that Y17 is more susceptible to electronic transitions (electron transport). Susceptible to electron leaps (UV absorption may be red-shifted); more chemically reactive (front orbitals are more likely to be involved in reactions). Electronegativity ( χ ): The electronegativity of Y17 (4.0817 eV) is higher than that of Az (3.8150 eV), suggesting that it is more capable of attracting electrons and may be more likely to participate in polar reactions (nucleophilic attack). The chemical potential ( µ = - χ ) is lower (-4.0817 eV), suggesting that Y17 is more thermodynamically stable. Electrophilicity index ( ω ): Y17 (4.2283 eV) is significantly higher than Az (3.0953 eV), suggesting that it is more capable of acting as an electron acceptor and may bind more efficiently to target proteins (bactericidal activity). Hardness ( η ) vs. Softness ( S ) Hardness: Az (2.3511 eV) > Y17 (1.9701 eV) Az is “harder”, with a more difficult to polarize electronic structure, greater resistance to charge transfer, and greater spatial site resistance Softness: Y17 (0.2538 eV − ¹) higher, more susceptible to charge transfer, suitable for catalytic or coordination reactions. Table 4 . We investigated the biological activities and mechanisms using frontline molecular orbital theory. As shown in Fig. 12 , the HOMO of Y17 is mainly concentrated on the β -amino alcohol structure; the LUMO is mainly located on the backbone and ring of flavonols with an energy difference of 0.1448 eV. The electron transfer is from β -amino alcohol to chalcone ring. The HOMO of Az is mainly located on the benzene ring close to the carbonyl group, on the methoxy and carbonyl structures, and the LUMO is mainly located on the cyano group and on the benzene ring attached to the cyano group, with an energy difference of 0.1728 eV. The electron transfer is from the benzene ring at one end to the benzene ring at the other end. From the above study, it is clear that the structure of current commercial drug Az has electron migration phenomenon indicating that the structure with electron migration behavior may be biologically active. Y17 both have electron migration phenomenon and lower energy difference value indicating that it has high reactivity. Figure 12 . 3 Conclusion In conclusion, twenty-one flavonol derivatives of β -amino alcohols were designed and synthesized. The target compound Y17 exhibited superior antifungal activity against P.c with EC 50 values of 5.2 µg/mL, respectively, which exceeded that of Az (98.5 µg/mL, and at a concentration of 200 µg/mL, Y17 showed significant protection in eggplant fruits (85.7%), which was superior to that of the Az (42.9%). Scanning electron microscopy and fluorescence microscopy analyses of Y17 -treated aspergillus oryzae mycelium of pepper revealed significant morphological changes, including mycelial shrinkage, structural deformation and membrane rupture. In addition, further studies showed that Y17 was able to disrupt the integrity of the cell membrane of the pathogenic fungus, affecting its lipid peroxidation and leading to the release of intracellular solutes. Chemical studies through computational simulations such as molecular dynamics (MD). The experimental results further confirmed that flavonol ketone derivatives containing β -amino alcohol moiety are promising candidates for the development of novel fungicides. 4. Materials and methods 4.1 Chemicals and Instruments Proton nuclear magnetic resonance (NMR) spectra were obtained by using the JEOL-JMTC-600(Japan, Hyogo), JEOL-ECX500 (Tokyo, Japan) and Bruker-ASCEND400 (Karlsruhe, Germany) spectrometers operated at room temperature with CDCl 3 as the solvent and tetramethylsilane as the internal standard. HRMS was conducted using Thermo Scientific Q Exactive (Thermo Scientific, Missouri). The morphology of the fungal mycelia was examined using a Nova Nano SEM 450 instrument (Field Electron and Ion Co.), an Olympus-BX53 fluorescence microscope (Olympus, Japan). The cell permeability was measured on the conductivity meter, Leici DDSJ-3O8F (Shanghai Instrument & Electric Science Instrument Co., Ltd., Shanghai, China), and the cell leakage was recorded on the N-5000 ultraviolet spectrophotometer (Shanghai Yoke Instrument Co., Ltd., Shanghai, China). The MDA assay kit was purchased from Beijing Solarbio Science & Technology Co. The reagents and solvents used in the experiment were purchased from Bositai Technology Co., Ltd. (Chongqing, China) or Shanghai Titan Chemical Co., Ltd. (Shanghai, China). The melting point measurements were obtained by using an X-4B melting point instrument (Shanghai INESA Co., Ltd., Shanghai, China). 4.2 Synthesis of intermediates and target compounds 4.2.1 Synthesis of intermediates 1–3 References [ 25 , 29 ], substituted 2'-Hydroxyacetophenone(1.66 mmol) and substituted benzaldehyde (1.66 mmol) were put into a 500 mL round-bottomed flask, ethanol was used as solvent, and hydroxyl aldehyde condensation took place at room temperature and under alkaline conditions, and at the end of the reaction it was precipitated out in iced water (pH = 4–5) to obtain intermediate 1 ; in a 500 mL round-bottomed flask, intermediate 1 (1 mmol) reacted with hydrogen peroxide under alkaline conditions to obtain intermediate 2 ; epoxy bromopropane (1.50 mmol) and intermediate 2 (2.20 mmol) in DMF solution, and after TCL detection the reaction was completed. In a 500 mL round bottom flask, intermediate 1 (1 mmol) and hydrogen peroxide under alkaline conditions, the ring-closing reaction to obtain intermediate 2 ; epoxybromopropane (1.50 mmol) and intermediate 2 (2.20 mmol) in DMF solution, TCL detection reaction after the end of the extraction with dichloromethane, concentrated under reduced pressure to get the intermediate 3 . 4.2.2 Synthesis of the target compounds Intermediate 3 (1 mmol), substituted ammonia (3 mmol), isopropanol as solvent and K 2 CO 3 (3 mmol) as acid-binding agent were put into a 100 mL round-bottomed flask and stirred at reflux at 80°C. After the completion of the reaction was monitored by TLC, the product was cooled down to room temperature, poured into iced water to precipitate, left to stand, filtered and dried [ 30 ]. The crude product was isolated and purified by column chromatography (methanol: dichloromethane = 1:10, v/v ) to obtain the target compounds Y1-Y21 . 4.2.3 Synthesis of compounds Y1 - Y21. 3-(3-(benzylamino)-2-hydroxypropoxy)-2-phenyl-4 H -chromen-4-one( Y1 ). White solid; m.p. 124.9-126.5 ℃; Yield 56%; 1 H NMR (500 MHz, CDCl 3 ) δ 8.27 (d, J = 8.0 Hz, 1H), 8.09 (m, 2H), 7.72 (m, 1H), 7.60–7.50 (m, 4H), 7.44 (t, J = 7.5 Hz, 1H), 7.14 (m, 2H), 6.65 (m, 3H), 5.33 (s, 1H), 4.31–3.88 (m, 4H), 3.24 (m, 2H). 13 C NMR (125 MHz, CDCl 3 ) δ 176.18, 157.07, 155.54, 148.46, 140.65, 134.20, 131.41, 130.56, 129.32, 128.96, 128.68, 126.05, 125.28, 123.73, 118.27, 117.62, 113.25, 76.69, 68.98, 45.99. HRMS(ESI) m/z [M + H] + Calcd for C 24 H 22 NO 4 388.15433 found 388.15308. 2-(4-bromophenyl)-3-(2-hydroxy-3-(phenylamino)propoxy)-4 H -chromen-4-one ( Y2 ). Orange oily; Yield 61%; 1 H NMR (500 MHz, CDCl 3 ) δ 8.29–8.23 (m, 1H), 8.00–7.95 (m, 2H), 7.78–7.58 (m, 4H), 7.50 (m, 2H), 7.14 (m, 2H), 6.68 (t, J = 7.5 Hz, 1H), 6.62 (m, 2H), 4.17–4.12 (m, 1H), 4.02 (m, 1H), 3.94 (m, J = 10.8, 7.0 Hz, 1H), 3.31 (m, 1H), 3.23–3.16 (m, 1H). 13 C NMR (125 MHz, CDCl 3 ) δ 176.01, 155.82, 155.43, 148.41, 140.72, 134.33, 132.25, 130.14, 129.44, 129.34, 126.09, 126.07, 125.40, 123.72, 118.21, 117.72, 113.28, 76.56, 69.04, 46.03. HRMS(ESI) m/z [M + H] + Calcd for C 24 H 21 O 4 NBr 466.06485, found 466.06363. 3-(2-hydroxy-3-(phenylamino)propoxy)-2-(4-methoxyphenyl)-4 H -chromen-4-one( Y3 ). Yellow solid; m.p. 112.8-113.7℃; Yield 47%; 1 H NMR (500 MHz, CDCl 3 ) δ 8.32–8.02 (m, 3H), 7.75–7.65 (m, 1H), 7.58–7.39 (m, 2H), 7.28–6.97 (m, 5H), 6.69–6.59 (m, 2H), 5.43 (s, 1H), 4.33–3.95 (m, 4H), 3.93–3.82 (m, 3H), 3.24 (m, 2H). 13 C NMR (125 MHz, CDCl 3 ) δ 175.96, 162.01, 157.03, 155.39, 148.50, 139.96, 133.94, 130.47, 129.31, 126.01, 125.12, 123.72, 122.78, 118.12, 117.59, 114.39, 113.25, 76.48, 69.02, 55.59, 46.02. HRMS(ESI) m/z [M + H] + Calcd for C 25 H 24 O 5 N 418.16490, found 418.16373. 2-(4-chlorophenyl)-3-(2-hydroxy-3-(phenylamino)propoxy)-4 H -chromen-4-one( Y4 ).Yellow solid; m.p. 141.1-142.5 ℃; Yield 51%; 1 H NMR (500 MHz, CDCl 3 ) δ 8.30–8.22 (m, 1H), 8.12–7.98 (m, 2H), 7.72 (ddd, J = 15, 8.0, 6.5 Hz, 1H), 7.49 (m, 4H), 7.14 (t, J = 7.5 Hz, 2H), 6.66 (m, 3H), 5.55–4.82 (m, 1H), 4.20–4.11 (m, 1H), 3.97 (m, 2H), 3.37–3.15 (m, 2H). 13 C NMR (126 MHz, CDCl 3 ) δ 175.96, 162.01, 157.03, 155.39, 148.50, 139.96, 133.94, 130.47, 129.31, 126.01, 125.12, 123.72, 122.78, 118.12, 117.59, 114.39, 113.25, 76.48, 69.02, 55.59, 46.02. HRMS(ESI) m/z [M + H] + Calcd for C 24 H 21 O 4 NCl 422.11536, found 422.11398. 3-(3-(ethylamino)-2-hydroxypropoxy)-2-phenyl-4 H -chromen-4-one (Y5) .Orange oily; Yield 47%; 1 H NMR (500 MHz, CDCl 3 ) δ 8.15 (dd, J = 8.0, 2.0 Hz, 1H), 8.04 (dd, J = 7.5, 2.5 Hz, 2H), 7.69–7.65 (m, 1H), 7.52–7.48 (m, 4H), 7.37 (t, J = 7.5 Hz, 1H), 5.09 (s, 2H), 4.21 (t, J = 5.0 Hz, 1H), 3.94 (dd, J = 10.5, 4.5 Hz, 1H), 3.88 (dd, J = 10.5, 5.5 Hz, 1H), 3.03 (d, J = 5.5 Hz, 2H), 2.97–2.84 (m, 2H), 1.28 (t, J = 7.5 Hz, 3H). 13 C NMR (125 MHz, CDCl 3 ) δ 176.06, 157.11, 155.45, 140.16, 134.24, 131.48, 130.24, 128.95, 128.67, 125.84, 125.26, 123.52, 118.24, 74.98, 67.29, 51.30, 44.16, 13.26. HRMS(ESI) m/z [M + H] + Calcd for C 20 H 22 O 4 N 340.15633, found 340.15403. 3-(3-(benzylamino)-2-hydroxypropoxy)-2-(p-tolyl)-4 H -chromen-4-one( Y6 ). Orange oily; Yield 57%; 1 H NMR (500 MHz, CDCl 3 ) δ 8.26 (dd, J = 8.0, 1.5 Hz, 1H), 8.00 (d, J = 8.5 Hz, 2H), 7.70 (m, 1H), 7.55 (d, J = 8.5 Hz, 1H), 7.43–7.40 (m, 1H), 7.33 (d, J = 8.0 Hz, 2H), 7.18–7.11 (m, 3H), 6.71–6.60 (m, 4H), 4.20–3.89 (m, 4H), 3.30 (dd, J = 12.5, 5.0 Hz, 1H), 3.20 (dd, J = 12.5, 6.5 Hz, 1H), 2.44 (s, 3H). 13 C NMR (125 MHz, CDCl 3 ) δ 176.09, 157.28, 155.47, 148.51, 142.03, 140.38, 134.08, 129.71, 129.39, 129.33, 128.63, 127.69, 125.98, 125.19, 123.72, 118.24, 117.59, 113.27, 76.50, 69.00, 46.04, 29.86, 21.77. HRMS(ESI) m/z [M + H] + Calcd for C 25 H 24 O 4 N. 402.16998, found 402.16885. 3-(3-(benzylamino)-2-hydroxypropoxy)-2-phenyl-4 H -chromen-4-one( Y7 ). Yellow oily; Yield 53%; 1 H NMR 1 H NMR (500 MHz, CDCl 3 ) δ 8.11–7.91 (m, 4H), 7.42 (m, 6H), 7.24 (m, 4H), 5.51 (s, 2H), 4.21 (d, J = 5.5 Hz, 1H), 4.05 (d, J = 6.5 Hz, 2H), 3.86–3.82 (m, 1H), 3.01 (m, 1H), 2.82 (d, J = 36 Hz, 2H). 13 C NMR (125 MHz, CDCl 3 ) δ 175.88, 162.82, 156.98, 155.36, 140.07, 134.45, 134.26, 131.48, 130.14, 129.66, 128.93, 128.84, 128.63, 125.72, 125.26, 123.44, 118.24, 74.83, 67.07, 52.75, 50.59. HRMS(ESI) m/z [M + H] + Calcd for C 25 H 24 O 4 N. 402.16998, found 402.16879 2-(4-(tert-butyl)phenyl)-3-(2-hydroxy-3-(phenylamino)propoxy)-4 H -chromen-4-one( Y8 ). Yellow oily; Yield 42%; 1 H NMR (400 MHz, CDCl3) δ 8.29 (dd, J = 8.0, 1.6 Hz, 1H), 8.06 (d, J = 8.8 Hz, 2H), 7.75–7.70 (m, 1H), 7.56 (dd, J = 8.4,6.0 Hz, 3H), 7.45 (t, J = 7.6 Hz, 1H), 7.16 (dd, J = 8.4, 7.2 Hz, 2H), 6.67 (m, 3H), 5.41 (s, 1H), 4.33–4.13 (m, 2H), 4.05 (dd, J = 10.8, 2.6 Hz, 1H), 3.96 (dd, J = 10.8, 7.2 Hz, 1H), 3.33 (dd, J = 12.4, 4.8 Hz, 1H), 3.21 (dd, J = 12.4, 6.4 Hz, 1H), 1.38 (s, 9H). 13 C NMR (100 MHz, CDCl 3 ) δ 176.07, 157.15, 155.43, 154.96, 148.40, 140.39, 133.98, 129.23, 128.36, 127.58, 125.97, 125.89, 125.11, 123.66, 118.13, 117.53, 113.16, 76.62, 68.94, 45.91, 35.09, 31.16. HRMS(ESI) m/z [M + H] + Calcd for C 28 H 30 O 4 N. 444.21693, found 444.21591. 3-(3-(benzylamino)-2-hydroxypropoxy)-2-(p-tolyl)-4 H -chromen-4-one (Y9) . White solid; m.p. 139.9-141.6℃; Yield 57%; 1 H NMR 1 H NMR (400 MHz, CDCl 3 ) δ 8.25 (dd, J = 8.0, 1.6 Hz, 1H), 8.00 (d, J = 8.4 Hz, 2H), 7.69 (m, 1H), 7.54 (m, 1H), 7.43–7.39 (m, 1H), 7.36–7.26 (m, 7H), 4.57–4.25 (m, 1H), 4.07–3.95 (m, 2H), 3.85 (dd, J = 10.8, 7.6 Hz, 1H), 3.78 (d, J = 1.6 Hz, 2H), 3.53–3.27 (m, 1H), 2.72 (d, J = 5.6 Hz, 2H), 2.44 (s, 3H). 13 C NMR (100 MHz, CDCl 3 ) δ 175.90, 156.97, 155.36, 141.77, 140.30, 140.12, 133.83, 129.53, 128.86, 128.52, 128.38, 128.19, 128.08, 127.71, 126.94, 125.90, 124.98, 123.72, 118.09, 76.61, 69.19, 53.98, 51.13, 21.64. HRMS(ESI) m/z [M + H] + Calcd for C 26 H 26 O 4 N. 416.18563, found 416.18393. 3-(3-(benzylamino)-2-hydroxypropoxy)-2-(4-chlorophenyl)-7-methoxy-4 H -chromen-4-one ( Y10 ). brown solid; m.p. 103.2-104.1℃; Yield 48%; 1 H NMR (500 MHz, CDCl 3 ) δ 8.14 (d, J = 9 Hz, 1H), 8.03–8.01 (m, 2H), 7.49–7.46 (m, 2H), 7.14 (dd, J = 8.5, 7.5 Hz, 2H), 6.99 (dd, J = 9.0, 2.5 Hz, 1H), 6.91 (d, J = 2.5 Hz, 1H), 6.68 (dd, J = 8.0, 7 Hz, 1H), 6.63–6.61 (m, 2H), 5.45 (s, 1H), 4.19–4.09 (m, 2H), 4.01–3.99 (m, 1H), 3.94 (d, J = 6.5 Hz, 2H), 3.92 (s, 3H), 3.89 (d, J = 5.5 Hz, 1H), 3.31–3.27 (m, 1H), 3.21–3.17 (m, 1H). 13 C NMR (126 MHz, CHLOROFORM- D ) δ 175.34, 164.71, 157.29, 155.16, 148.46, 140.52, 137.31, 129.82, 129.32, 129.22, 129.07, 127.39, 117.65, 117.50, 115.23, 113.26, 100.05, 76.62, 69.04, 56.07, 46.04. HRMS(ESI) m/z [M + H] + Calcd for C 26 H 25 O 5 NCl. 466.14158, found 466.14111. 3-(3-(benzylamino)-2-hydroxypropoxy)-2-(4-chlorophenyl)-4 H -chromen-4-one (Y11) . Brown oily; Yield 35%; 1 H NMR (400 MHz, CDCl 3 ) δ 8.24 (dd, J = 8.1, 1.7 Hz, 1H), 8.07 (d, J = 8.8 Hz, 2H), 7.74–7.70 (m, 1H), 7.49 (d, J = 8.7 Hz, 2H), 7.35–7.28 (m, 7H), 4.61 (s, 1H), 4.05–3.98 (m, 2H), 3.95–3.84 (m, 2H), 3.78 (d, J = 1.5 Hz, 2H), 2.75–2.69 (m, 2H). 13 C NMR (101 MHz, CDCl 3 ) δ 175.80, 155.44, 155.29, 140.60, 139.98, 137.35, 134.10, 129.94, 129.10, 128.43, 128.19, 127.02, 125.95, 125.20, 123.68, 118.08, 76.50, 69.16, 53.94, 51.06.HRMS(ESI) m/z [M + H] + Calcd for C 25 H 23 O 4 NCl. 436.13319, found 436.12988. 3-(3-(hexylamino)-2-hydroxypropoxy)-2-phenyl-4 H -chromen-4-one (Y12) . Brown oily; Yield 40%; 1 H NMR (600 MHz, CDCl 3 ) δ 8.20 (dd, J = 8.4, 1.8 Hz, 1H), 8.09–8.05 (m, 2H), 7.69 (m, 1H), 7.55–7.50 (m, 4H), 7.40 (m, 1H), 4.21 (s, 2H), 4.09 (m, 1H), 3.96 (dd, J = 10.2, 3.6 Hz, 1H), 3.86 (dd, J = 10.2, 6.6 Hz, 1H), 2.86 (d, J = 5.4 Hz, 2H), 2.74–2.65 (m, 2H), 1.60–1.51 (m, 2H), 1.30–1.23 (m, 6H), 0.84 (t, J = 6.6 Hz, 3H). 13 C NMR (150 MHz, CDCl 3 ) δ 176.05, 156.97, 155.49, 140.40, 134.13, 131.37, 130.45, 128.90, 128.67, 125.94, 125.20, 123.68, 118.22, 75.80, 68.17, 51.75, 49.91, 31.69, 28.95, 26.90, 22.65, 14.12. HRMS(ESI) m/z [M + H] + Calcd for C 24 H 30 O 4 N. 396.21693, found 396.21567. 3-(3-(ethylamino)-2-hydroxypropoxy)-2-(4-fluorophenyl)-4 H -chromen-4-one( Y13 ). brown oily; Yield 47%; 1 H NMR (500 MHz, CDCl 3 ) δ 8.07–7.96 (m, 2H), 7.72 (dd, J = 8.0, 3.0 Hz, 1H), 7.56–7.45 (m, 4H), 7.40 (m, 1H), 4.37 (t, J = 5.5 Hz, 1H), 4.00–3.89 (m, 2H), 3.37–3.16 (m, 4H), 1.45 (t, J = 7.5 Hz, 3H). 13 C NMR (125 MHz, CDCl 3 ) δ 175.17, 160.45, 158.48, 157.51, 151.74, 139.54, 131.78, 129.78, 129.06, 128.69, 124.65, 124.59, 122.81, 122.60, 120.50, 120.44, 110.49, 110.30, 73.70, 65.82, 50.91, 44.14, 11.46. 19 F NMR (565 MHz, CDCl 3 ) δ -120.31. HRMS(ESI) m/z [M + H] + Calcd for C 20 H 21 FNO 4 . 358.14491, found 358.14377. 3-(3-((4-fluorobenzyl)-l2-azaneyl)-2-hydroxypropoxy)-2-phenyl-4 H -chromen-4-one( Y14 ). Brown oily; Yield 54%; 1 H NMR (500 MHz, CDCl 3 ) δ 8.23 (dd, J = 8.0, 2.0 Hz, 1H), 8.08 (m, 2H), 7.70 (m, 1H), 7.56–7.50 (m, 4H), 7.41 (t, J = 7.5 Hz, 1H), 7.27 (dd, J = 8.5, 5.5 Hz, 2H), 6.95 (m, 2H), 4.05 (s, 1H), 3.96 (m, 1H), 3.87–3.74 (m, 4H), 2.74 (d, J = 4.5 Hz, 2H). 13 C NMR (125 MHz, CHLOROFORM- D ) δ 176.10, 163.07, 161.12, 156.91, 155.49, 140.54, 135.05, 134.14, 131.35, 130.55, 130.09, 130.03, 128.90, 128.67, 125.97, 125.22, 123.71, 118.24, 115.39, 115.22, 76.47, 68.91, 53.12, 51.09. 19 FNMR (471 MHz, CDCl 3 ) δ-115.56. HRMS (ESI)m/z. [M + H] + Calcd for C 25 H 23 FNO 4 . 420.16056, found 420.15924 2-(4-bromophenyl)-3-(3-(hexylamino)-2-hydroxypropoxy)-7-methoxy-4 H -chromen-4-one( Y15 ). White solid; m.p. 131.2-132.1℃; Yield 36%; 1 H NMR (500 MHz, CDCl 3 ) δ 8.29–8.23 (m, 1H), 8.00–7.95 (m, 2H), 7.78–7.58 (m, 4H), 7.50 (m, 2H), 7.14 (m, 2H), 6.68 (t, J = 7.5 Hz, 1H), 6.62 (m, 2H), 4.17–4.12 (m, 1H), 4.02 (m, 1H), 3.94 (m, J = 10.8, 7.0 Hz, 1H), 3.31 (m, 1H), 3.23–3.16 (m, 1H). 13 C NMR (125 MHz, CDCl 3 ) δ 175.21, 164.70, 157.23, 155.15, 140.12, 132.16, 129.99, 129.27, 127.16, 125.86, 117.32, 115.30, 99.95, 75.26, 67.55, 56.07, 51.60, 49.67, 31.60, 28.31, 26.82, 22.64, 14.13. HRMS(ESI) m/z [M + H] + Calcd for C 25 H 31 BrNO 5 . 504.13801, found 504.14053 3-(3-(benzylamino)-2-hydroxypropoxy)-7-methoxy-2-(p-tolyl)-4 H -chromen-4-one(Y16). White solid; m.p. 112.8-113.7℃; Yield 57%; 1 H NMR (500 MHz, CDCl 3 ) δ 8.05 (d, J = 9.0 Hz, 1H), 7.92 (d, J = 8.5 Hz, 2H), 7.59 (d, J = 8.5 Hz, 2H), 7.31–7.22 (m, 6H), 6.93 (dd, J = 9.0, 2.5 Hz, 1H), 6.86 (d, J = 2.5 Hz, 1H), 4.06–4.03 (m, 1H), 3.93 (dd, J = 10.5, 3.0 Hz, 1H), 3.88 (s, 3H), 3.86–3.75 (m, 4H), 2.78–2.68 (m, 2H). 13 C NMR (125 MHz, CDCl 3 ) δ 175.20, 164.60, 157.18, 154.95, 140.43, 139.51, 132.09, 129.98, 128.54, 128.43, 127.26, 127.22, 125.70, 117.47, 115.17, 99.97, 76.40, 69.00, 56.07, 53.88, 51.14. HRMS(ESI) m/z [M + H] + Calcd for C 26 H 25 BrNO 5 . 510.09106, found 510.08975 3-(3-(hexylamino)-2-hydroxypropoxy)-2-(p-tolyl)-4 H -chromen-4-one( Y17 ). Orange oily; Yield 61%; 1 H NMR (400 MHz, CDCl 3 ) δ 8.21 (dd, J = 8.0, 1.6 Hz, 1H), 8.03 (d, J = 8.4 Hz, 2H), 7.79–7.70 (m, 1H), 7.59 (d, J = 8.5 Hz, 1H), 7.45 (t, J = 7.6 Hz, 1H), 7.35 (d, J = 8.0 Hz, 2H), 4.88 (s, 2H), 4.29 (t, J = 4.8 Hz, 1H), 3.97 (m, 2H), 3.28 (m, 2H), 3.00 (m, 2H), 2.44 (s, 3H), 1.88 (m, 2H), 1.45–1.28 (m, 6H), 0.92–0.85 (m, 3H). 13 C NMR (100 MHz, CDCl 3 ) δ 176.09, 157.64, 155.43, 142.32, 139.51, 134.25, 129.72, 128.53, 127.12, 125.74, 125.26, 123.31, 118.21, 73.50, 66.53, 51.84, 49.41, 31.34, 26.95, 26.55, 22.52, 21.66, 14.02. HRMS(ESI) m/z [M + H] + Calcd for C 25 H 32 NO 4 . 410.23258, found 410.23117. 3-(3-(hexylamino)-2-hydroxypropoxy)-2-(2-methoxyphenyl)-4 H -chromen-4-one( Y18 ). White solid; m.p. 136.7-137.5℃; Yield 64%; 1 H NMR (500 MHz, CDCl 3 ) δ 8.20 (dd, J = 8.0, 1.5 Hz, 1H), 7.70 (m, 1H), 7.52–7.40 (m, 4H), 7.10–7.01 (m, 2H), 4.16 (m, 1H), 3.91–3.82 (m, 5H), 3.27–3.15 (m, 2H), 3.09–2.97 (m, 2H), 1.90 (p, J = 8.0 Hz, 2H), 1.41–1.27 (m, 6H), 0.89–0.82 (m, 3H). 13 C NMR (100 MHz, CDCl 3 ) δ 176.09, 157.64, 155.43, 142.32, 139.51, 134.25, 129.72, 128.53, 127.12, 125.74, 125.26, 123.31, 118.21, 73.50, 66.53, 51.84, 49.41, 31.34, 26.95, 26.55, 22.52, 21.66, 14.02. HRMS(ESI) m/z [M + H] + Calcd for C 25 H 32 NO 5 . 426.22750, found 426.22601. 2-(3-bromophenyl)-3-(3-(hexylamino)-2-hydroxypropoxy)-4 H -chromen-4-one( Y19 ). Orange oily; Yield 31%; 1 H NMR (500 MHz, CDCl 3 ) δ 8.20–8.05 (m, 3H), 7.75–7.67 (m, 1H), 7.63–7.51 (m, 2H), 7.44–7.36 (m, 2H), 5.42 (s, 2H), 4.32 (t, J = 5.0 Hz, 1H), 3.97 (t, J = 5.5 Hz, 2H), 3.24 (d, J = 5.0 Hz, 2H), 2.99 (d, J = 8.5 Hz, 2H), 1.82–1.79 (m, 1H), 1.36 (s, 1H), 1.26 (m, 6H), 0.86–0.81 (m, 3H). 13 C NMR (125 MHz, CDCl 3 ) δ 175.89, 155.41, 155.28, 140.26, 134.55, 134.37, 132.04, 131.11, 130.68, 127.58, 125.87, 125.49, 123.45, 122.92, 118.30, 74.01, 66.41, 51.47, 49.34, 31.39, 26.87, 26.58, 22.57, 14.07. HRMS(ESI) m/z [M + H] + Calcd for C 24 H 29 BrNO 4 . 474.12745, found 474.12610. 3-3-(((4-fluorobenzyl)amino)-2-hydroxypropoxy)-2-(4-methoxyphenyl)-4 H -chromen-4-one ( Y20 ). White solid; m.p. 146.3-147.1℃; Yield 64%; 1 H NMR (500 MHz, CDCl 3 ) δ 8.05 (t, J = 8.5 Hz, 3H), 7.68–7.64 (m, 3H), 7.51 (d, J = 8.5 Hz, 1H), 7.38 (d, J = 7.5 Hz, 1H), 7.03–6.97 (m, 4H), 4.37 (dd, J = 25.0, 11.0 Hz, 4H), 3.94–3.86 (m, 3H), 3.82 (s, 3H), 3.36 (d, J = 5.0 Hz, 1H), 2.37 (s, 1H). 13 C NMR (125 MHz, CDCl 3 ) δ 176.0, 175.8, 164.3, 162.3, 162.4, 157.6, 155.4, 155.3, 142.5, 139.4, 138.9, 134.38, 134.27, 132.84, 132.77, 130.56, 129.81, 128.58, 126.96, 126.72, 125.64, 125.59, 125.39, 125.34, 123.23, 123.18, 121.96, 118.28, 118.18, 116.18, 116.01, 114.56, 73.39, 73.26, 65.80, 55.60, 51.50, 50.56, 50.43, 21.69. 19 FNMR (471 MHz, CDCl 3 ) δ -111.52. HRMS (ESI) m/z [M + H] + Calcd for C 26 H 25 NO 5 F. 450.17113, found 450.16974. 3-(3-(hexylamino)-2-hydroxypropoxy)-2-(4-methoxyphenyl)-4 H -chromen-4-one( Y21 ). Yellow oily; Yield 53%; 1 H NMR (500 MHz, CDCl 3 ) δ 8.11 (m, 3H), 7.71–7.67 (m, 1H), 7.53 (d, J = 8.5 Hz, 1H), 7.38 (m, 1H), 7.01 (d, J = 9.0 Hz, 2H), 4.38–4.32 (m, 1H), 3.96 (t, J = 5.0 Hz, 2H), 3.85 (d, J = 1.0 Hz, 3H), 3.38 (d, J = 5.0 Hz, 2H), 3.10 (d, J = 8.5 Hz, 2H), 1.90 (d, J = 2.5 Hz, 1H), 1.49–1.12 (m, 9H), 0.86–0.83 (m, 3H). 13 C NMR (125MHz, CDCl 3 ) δ 175.95, 162.31, 157.49, 155.35, 138.99, 134.24, 130.58, 125.69, 125.28, 123.25, 122.07, 118.19, 114.56, 73.20, 65.96, 55.61, 51.72, 49.32, 31.30, 26.46, 26.30, 22.55, 14.07. HRMS(ESI) m/z [M + H] + Calcd for C 25 H 32 NO 5 . 426.22750, found 426.22626. 4.3. Plant Pathogenic Fungi 4.3.1. Fungal sources The fungi used in this study were all collected from natural habitats and kindly provided by the National Key Laboratory of Green Pesticide. 4.3.2. Cultivation of fungi The medium was boiled in the ratio of potato: glucose: agar = 10: 10: 9, dispensed in 250 mL conical flasks and sterilized using autoclave at 121°C for 21 min, after completion of sterilization the cake which had been activated for 30 min was inoculated in the cooled medium and placed in the incubator for 5 d [ 7 , 31 ]. 4.4 Antifungal test 4.4.1 Antifungal activity in vitro The fungicidal activity of the target compounds was determined by mycelial growth rate [ 32 ]. The antifungal activity of Y1-Y21 at a concentration of 100 µg/mL was determined using mycelial growth method. The medium was prepared according to the ratio of potato dextrose agar (PDA), dispensed in 50 mL conical flasks and sterilized at 121 degrees celsius for 21 min. The compounds were dissolved in dimethyl sulfoxide (DMSO) and mixed with PDA to give a final concentration of 100 µg/mL, PDA containing the drug solution was poured into petri dishes and each set was repeated three times. The EC 50 of some of the compounds was further determined after screening the compounds with better activity. I —Inhibition rate C —Blank control mycelium diameter T — Mycelial diameter of drug treatment group 4.4.2 Antifungal activity in vivo The curative and protective efficacy of Y17 against P.c was assessed in vivo , following the method reported in the literature. DMSO dissolved Y17 was formulated in different concentrations (100 and 200 µg/mL), fresh eggplant fruits were used as experimental material, the commercial drug, Az was used as a positive control group, and sterile distilled water (containing 0.2 mL of DMSO and 0.1% Tween − 80) was used as negative control. Curative activity was tested by punching a 5 mm hole in the surface of the eggplant, placing a cake of the fungus, and spraying the cake with Y17 solution after 24 h. Protective activity was tested by punching a 5 mm hole in the surface of the eggplant, spraying Y17 solution, and placing a cake of the fungus after 24 h. The area of infected eggplant fruits was measured after 5 d using the cross method [ 33 , 34 ]. C —Control effect ACK —Blank control group lesion diameter A —The diameter of the lesion after medication treatment. 4.5. Scanning electron microscope observation P.c was inoculated on potato medium at 28℃ for 5 d, selected mycelia with a diameter of 5mm at the edge of the petri dish, picked 2–3 cakes in 20 mL PDA medium, cultured at 28℃, 180 r for 3–4 d, then added Y17 with DMSO solvent solution to make the final concentration of 0, 12.5, 25 µg/mL, under the same conditions, cultured for another 24 h. A small number of bacteria was picked and fixed with glutaraldehyde for 12 h, and the medium and other impurities were washed away with PBS. After replacing the medium with 30, 50, 70, 90, 100% for 2 times respectively, the bacteria were fixed with tert-butanol for 30 min (refrigerator freezing) and freeze-dried for 4 h. The bacteria were placed on conductive adhesive, sprayed with gold and the morphology of hyphae was observed in the scanning electron microscope [ 35 ]. 4.6. FM Observation. P.c was inoculated on PDA medium at 28℃ for 5 d, selected mycelia with a diameter of 5mm at the edge of the petri dish, picked 2–3 cakes in 20 mL PDA medium, incubated at 28℃, 180r for 3–4 d, and then added the solution of Y17 solvented with DMSO to make the final concentration of 0, 50, 100 µg/mL, and incubated for another 24 h under the same conditions. A small amount of mycelium was picked and washed with PBS for 3 times, incubated with PI stain at 37°C for 15 min, then washed off the stain using PBS, and the mycelium was placed on slides for sampling with a sterile inoculation needle, and the mycelial morphology was observed under a fluorescence microscope [ 36 ]. 4.7. ROS accumulation Referring to the method in the literature [ 37 ], the mycelium with the diameter of 5 mm at the edge of the petri dish was selected, and 2 cakes were picked in 25 mL PDA medium, incubated at 28℃, 180r for 2–3 d, then Y17 solution was added to make the final concentration of 0, 50, 100 µg/mL, and incubated under the same conditions for another 24 h. A small amount of mycelium was picked and incubated with the staining agent for 30 min at 37℃, and the staining agent was washed away with PBS [ 38 ]. The stain was washed off, and the mycelium was placed on a slide with a sterile inoculation needle to make a sample, and the mycelial morphology was observed under a fluorescence microscope. 4.8 Determination of leakage of intracellular solutes Referring to the experimental method in the literature [ 39 ], a 5-mm cake of mycelium was selected and incubated with PDA medium at 28°C, 180 r for 4–5 d, then washed with sterile water for three times and filtered; 100 mg of mycelium was weighed into 20 mL of sterile water, and DMSO-solubilized Y17 was added to obtain mycelial suspensions with solution concentrations of 12.5, 25, 50, and 100 µg/mL, respectively. The obtained suspensions were incubated at 28°C for 10 h. The absorbance of the supernatant was measured at 260 and 280 nm using a UV-Vis spectrophotometer. 4.9 Determination of cell membrane permeability Referring to the experimental method in the literature [ 40 ], 5mm cakes of mycelium were selected and incubated with PDA medium at 28°C, 180 r for 4–5 d, then 200 mg of mycelium was added to 20 mL of sterile water with DMSO-solubilized Y17 , to obtain mycelial suspensions with solution concentrations of 12.5, 25, 50, and 100 µg/mL, respectively, and the solutions were tested for their electrical conductivities at 1h intervals. After 6 h of testing, boiled in water at 100°C for 60 min, cooled, and measured its conductivity. 4.10 Cell dry weight experiment Referring to the literature method [ 41 ], Capsicum blight fungus was inoculated on PDA medium for 5 d at 28°C. Mycelia with a diameter of 5 mm at the edge of the Petri dish were selected, and 2 cakes were picked in 20 mL of PDA medium, and then Y17 solution solubilized with DMSO was added to make the final concentration of 0, 3.125, 6.25, 12.5, and 25 µg/mL, after incubation at 28°C, 180 r for 3–4 d, the sample was washed 3–4 times with PBS, filtered, dried at 50°C and weighed. 4.11 Determination of MDA content Mycobacterium chiliensis was cultured in sterile PDA medium and incubated in a constant temperature shaker at 25°C for 2–3 d. Under the same conditions, the strains were incubated in Y17 solution containing different concentrations (0, 12.5, 25, 50, and 100 µg/mL) for 1 d, washed with sterile water, and the mycelia were collected and freeze-dried for 6 h. The dry weight of 100mg of mycelium under different treatments was weighed and experiments were carried out according to the kit instructions to determine the absorbance of the samples at 532 and 600 nm [ 37 ]. 4.12 Experimental safety tests Referring to previous literature reports [ 42 ]. Pepper seeds were soaked with different concentrations of Y17 (0, 100, 200 and 400 µg/mL). The germination of chili pepper seeds was observed every 3 d, and the leaves and roots of chili pepper seedlings were sprayed with the above concentrations of Y17 for one consecutive week to observe the growth of the seedlings. 4.13 Energy calculation Calculate the molecular structure using Chemdraw. Then, single point energies were calculated by Gaussian view to obtain the desired energy parameters [ 16 ]. Global reactivity descriptors HOMO and LUMO energies were used to define global reactivity descriptors such as softness ( S ), global hardness ( η ), chemical potential (µ), electronegativity ( χ ), and global electrophilicity index ( ω ) using the following relational equation: Declarations Acknowledgement Authors gratefully acknowledge the Science Foundation of Guizhou Province (No. ZK2024008), Chinese Academy of Tropical Agricultural Sciences for Science and Technology Innovation Team of National Tropical Agricultural Science Center (No. CATASCXTD202410). Supporting information The Supporting Information includes the synthesis and characterization data, and NMR, HRMS spectrogram for the target compounds. Authors' contributions The current study is an outcome of constructive discussion with W.X; Revised in full by X.H.R; Y.J.Q and C.M.H performed synthesis and characterization experiments on the target compounds; Y.J.Q,Q.Z and Q.X.H completed experiments on preliminary screening and mechanism of action studies of antiviral activity; Y.J.Q, C.M.H and Q.Z carried out the NMR, and HRMS spectral analyses; Q.X.H prepared figures 1-8 and table 1-2; D,S were involved in the drafting of the manuscript and revising the manuscript. All authors read and approved the final manuscript. Conflicts of interest The authors declare that there are no competing financial interests. References Isinkaye FO, Olusanya MO, Singh PK (2024) Deep learning and content-based filtering techniques for improving plant disease identification and treatment recommendations: A comprehensive review. 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Molecular Diversity. 2025-01-22.https://doi.org/10.1007/s11030-025-11109-6 Tables Tab. 1 Antifungal activities of Y1 - Y21 at 100 μg/mL in vitro a Compd Inhibition rate (%) b P . c S.s F.o P.s R.s B.d F.g F.m F.oc Y1 28.1±3.1 38.8±2.3 31.5±3.4 55.7±2.3 27.1±0.6 14.9±1.8 34.7±2.5 34.7±1.1 13.6±2.2 Y2 13.2±2.4 8.8±1.2 12.0±1.8 34.4±0.8 29.0±1.7 24.9±1.6 12.4±1.1 21.9±2.3 12.8±2.6 Y3 14.5±3.0 14.2±3.5 22.0±3.6 41.8±3.5 53.4±4.9 38.2±4.2 32.2±4.3 31.1±2.5 9.9±4.6 Y4 17.8±2.6 26.7±2.7 19.1±2.3 38.5±1.2 56.1±2.0 24.5±2.9 20.2±2.6 18.6±3.2 19.4±1.2 Y5 28.1±3.4 43.8±3.4 24.9±4.1 50.5±2.4 58.0±3.1 6.4±4.2 26.4±3.0 9.9±2.7 15.9±4.3 Y6 40.5±3.3 40.0±4.2 30.2±2.2 67.3±4.2 45.0±0.8 42.2±2.6 36.6±1.8 56.4±1.5 59.7±2.2 Y7 86.8±1.1 10.4±2.2 11.8±3.3 20.5±2.6 59.5±2.5 14.5±4.7 21.9±4.1 55.4±3.7 5.4±2.9 Y8 62.8±2.2 - - 18.7±4.2 51.9±1.8 - 34.3±1.8 14.9±3.6 - Y9 89.5±0.9 12.5±3.4 32.4±0.9 - 17.9±0.8 11.7±2.2 - 55.8±2.9 19.8±2.3 Y10 69.0±1.8 36.7±2.1 13.7±1.8 27.8±3.8 41.2±1.1 2.8±1.8 14.5±2.7 46.3±1.8 12.4±3.6 Y11 96.7±1.1 93.3±1.9 61.0±2.3 - 39.3±2.2 34.1±1.3 40.9±2.9 82.6±2.0 27.3±1.8 Y12 90.5±0.9 96.3±3.1 43.2±1.7 93.8±0.8 64.1±3.3 66.7±1.6 10.7±3.4 40.5±1.9 36.0±0.9 Y13 53.7±3.7 38.8±2.3 31.5±3.4 25.6±1.5 67.6±2.7 - - 35.3±2.8 27.7±2.9 Y14 33.1±2.4 61.5±1.7 - 36.6±2.2 14.5±4.2 5.6±1.6 35.5±2.8 63.2±3.2 - Y15 93.5±2.7 54.1±3.4 68.9±2.3 78.4±3.8 75.6±2.1 75.9±1.4 59.5±2.7 57.4±1.0 67.8±2.0 Y16 92.6±2.0 96.3±1.2 75.1±2.0 - 69.1±1.1 57.0±0.5 33.6±0.7 40.2±1.3 35.5±2.4 Y17 100.0±0.0 95.4±3.3 41.5±5.4 96.7±1.1 77.5±3.0 82.4±2.1 70.2±3.9 69.0±3.6 34.7±3.5 Y18 97.1±0.9 83.8±1.2 70.1±1.5 91.6±0.8 60.7±2.7 52.9±0.9 36.5±2.0 67.2±3.4 30.2±1.2 Y19 91.3±0.3 97.5±0.4 60.1±3.7 96.3±1.0 85.9±1.5 62.2±2.2 31.4±1.5 77.3±2.9 30.6±2.0 Y20 71.5±1.1 98.2±0.6 63.9±9.4 93.4±0.7 11.5±0.8 66.0±0.7 74.4±1.8 79.0±1.2 45.5±1.4 Y21 92.6±2.4 87.1±2.1 55.0±2.0 87.5±1.2 60.3±2.1 74.3±1.1 67.4±0.9 67.5±0.4 38.0±2.0 Az c 48.9±2.4 88.8±2.3 78.3±2.1 60.6±3.7 76.3±3.1 69.1±2.7 72.7±2.4 64.7±1.6 60.6±3.7 a Vales are mean ± SD of three replicates. b R.s ( R.solani ), S.s ( Ssclerotiorm ) , F.g ( F.graminearm ) , P.c ( P.capsici ) , F.oc ( F.oxysporum cucumerinum ) , P.s ( Phomopsis.sp), F.o ( F.oxysporum ) , F.m ( T. Botrytis cinerea ), B.d (B. dothidea) c The commercial antifungal agent Azoxystrobin. Tab. 2 The EC 50 values of some target compounds a . Compd Phytopathogen Regression equation R 2 EC 50 (μg/mL) Y14 P.c y = 2.7324x + 2.3199 0.9939 9.5 Y17 P.c y = 1.9978x + 3.5650 0.9911 5.2 Y18 P.c y = 2.1142x + 2.9669 0.9554 9.1 Y21 P.c y = 3.1658x + 2.2059 0.9759 7.6 AZ b P.c y = 1.9578x + 1.0977 0.9833 98.5 Y12 S.s y = 2.8155x + 1.8263 0.9465 13.4 Y16 S.s y = 2.7066x + 1.4513 0.9925 20.47 Y17 S.s y = 3.0669x + 1.2437 0.9684 16.8 Y18 S.s y = 3.4447x + 0.3402 0.9248 22.5 Y20 S.s y = 2.6969x + 2.2423 0.9799 10.5 AZ b S.s y = 1.2227x + 2.9211 0.9348 50.1 Y12 P.s y = 2.7915x + 1.3955 0.9815 19.5 Y14 P.s y = 2.9388x + 0.7153 0.9783 28.7 Y17 P.s y = 2.3055x + 2.0313 0.9734 19.3 Y18 P.s y = 2.7725x + 0.9446 0.9602 29.0 Y20 P.s y = 2.6263x + 1.6147 0.9718 19.4 AZ b P.s y = 1.4157x + 2.5526 0.9448 53.5 a Vales are mean ± SD of three replicates. b The commercial antifungal agent Azoxystrobin. Tab. 3 The Protective and curative activities of Y17 and Az against P.c in vivo a Curativeactivity Protectiv e activity Compd Concentration Lesionlength a (mm±SD) Controlling efffcacy (%) Lesionlength a (mm±SD) Controlling efffcacy(%) Y17 200.0 15.4±0.9 77.8 9.0±1.3 85.7 100.0 3.0±1.1 38.8 24.3±1.5 45.7 Az b 200.0 39.4±1.5 35.5 25.7±0.8 42.9 100.0 42.4±0.6 22.2 32.1±0.9 22.9 Control 0.0 50.0±0.1 39.4±1.1 a Values are mean ± SD of three replicates. b The commercial antifungal agent Azoxystrobin. Tab. 4 Physicochemical property comparison of Y17 and Az Parameter Az Y17 MV a 403.39 409.53 Ars a 2 2 E Total b -1389.0443 -1327.1366 E HOMO b (Eh) -0.2266 -0.2224 E LUMO b (Eh) -0.0538 -0.0776 E a b (Eh) 0.1728 0.1448 χ c (eV) 3.8150 4.0817 μ c (eV) -3.8150 -4.0817 η c (eV) 2.3511 1.9701 S c (eV) 0.2127 0.2538 ω c (eV) 3.0953 4.2283 a Calculate the molecular structure using Chemdraw b Gaussian view were used to obtain the molecular energy and energy orbital values. c The unit is converted from Hartree (Eh) to eV, 1 Eh = 27.211 eV Scheme 1 Scheme 1 is available in the Supplementary Files section. Additional Declarations No competing interests reported. Supplementary Files supporinformationY3.3.pdf GraphicalAbstract.png Scheme1.png Scheme 1 Schematic representation of the synthesis of target compounds Y1–Y21 Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. 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Guizhou University","correspondingAuthor":false,"prefix":"","firstName":"Chunmei","middleName":"","lastName":"Hu","suffix":""},{"id":479204438,"identity":"ae6816bc-1654-4f1a-b070-5add5cbef59a","order_by":2,"name":"Qing Zhou","email":"","orcid":"","institution":"Center for R\u0026D of Fine Chemicals of Guizhou University","correspondingAuthor":false,"prefix":"","firstName":"Qing","middleName":"","lastName":"Zhou","suffix":""},{"id":479204439,"identity":"1858b433-25d5-4421-a69e-95e291d3136b","order_by":3,"name":"Qingxue Hu","email":"","orcid":"","institution":"Center for R\u0026D of Fine Chemicals of Guizhou University","correspondingAuthor":false,"prefix":"","firstName":"Qingxue","middleName":"","lastName":"Hu","suffix":""},{"id":479204440,"identity":"86aea134-933b-4459-b1b3-f4ee94d74ec0","order_by":4,"name":"Dan Shen","email":"","orcid":"","institution":"Center for R\u0026D of Fine Chemicals of Guizhou 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University","correspondingAuthor":true,"prefix":"","firstName":"Wei","middleName":"","lastName":"Xue","suffix":""}],"badges":[],"createdAt":"2025-06-27 08:23:07","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-6989432/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-6989432/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":85940942,"identity":"21247bbb-0ca6-4aa8-a995-06a960b14aeb","added_by":"auto","created_at":"2025-07-03 11:41:03","extension":"png","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":66454,"visible":true,"origin":"","legend":"\u003cp\u003e\u003cstrong\u003eDesign of the target compounds.\u003c/strong\u003e\u003c/p\u003e","description":"","filename":"1.png","url":"https://assets-eu.researchsquare.com/files/rs-6989432/v1/2627b25e46adfa97da64d44c.png"},{"id":85940949,"identity":"86b54806-0a8f-4410-b9e9-03d2d95dc6a6","added_by":"auto","created_at":"2025-07-03 11:41:03","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":815992,"visible":true,"origin":"","legend":"\u003cp\u003eMycelium growth of\u003cem\u003e P.c\u003c/em\u003e strain after treating with of \u003cstrong\u003eY17\u003c/strong\u003e and \u003cstrong\u003eAz\u003c/strong\u003e\u003c/p\u003e","description":"","filename":"2.png","url":"https://assets-eu.researchsquare.com/files/rs-6989432/v1/051fe0ff6bb7f9a57e58ef38.png"},{"id":85941439,"identity":"d759a776-f138-409b-9cc2-b1307a01b71c","added_by":"auto","created_at":"2025-07-03 11:49:03","extension":"png","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":299674,"visible":true,"origin":"","legend":"\u003cp\u003eThe protective and curative effect of \u003cstrong\u003eY17 \u003c/strong\u003eand \u003cstrong\u003eAz\u003c/strong\u003e against \u003cem\u003eP.c\u003c/em\u003e on eggplant.\u003c/p\u003e","description":"","filename":"3.png","url":"https://assets-eu.researchsquare.com/files/rs-6989432/v1/2ae4a77d4707c9d5948731a5.png"},{"id":85941433,"identity":"9001f90c-2b26-4a31-94ec-3a37eb077fcd","added_by":"auto","created_at":"2025-07-03 11:49:02","extension":"png","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":993434,"visible":true,"origin":"","legend":"\u003cp\u003eSEM images of the mycelium of \u003cem\u003eP.c\u003c/em\u003e after treatment with \u003cstrong\u003eY17\u003c/strong\u003e. (a):0 μg/mL, (b):25 μg/mL and (c): 12.5 μg/mL. Scale bar for (a−c) were 50 μm.\u003c/p\u003e","description":"","filename":"4.png","url":"https://assets-eu.researchsquare.com/files/rs-6989432/v1/9faf9dee45c53fc3bee5f51f.png"},{"id":85942415,"identity":"1e3ceeb0-0885-4271-9dbd-a90595ae8c44","added_by":"auto","created_at":"2025-07-03 11:57:03","extension":"png","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":417774,"visible":true,"origin":"","legend":"\u003cp\u003eMycelium after staining treatment by fluorescence microscopy. (d, d-1): 0 μg/mL; (e, e-1): 50 μg/mL; (f, f-1):100 μg/mL; magnification: 40 × 10; scale for 10 μm.\u003c/p\u003e","description":"","filename":"5.png","url":"https://assets-eu.researchsquare.com/files/rs-6989432/v1/682144304cba67f476bd1f9d.png"},{"id":85942412,"identity":"18e87c62-a82b-4bb2-a0a3-a27ff805f245","added_by":"auto","created_at":"2025-07-03 11:57:03","extension":"png","order_by":6,"title":"Figure 6","display":"","copyAsset":false,"role":"figure","size":807753,"visible":true,"origin":"","legend":"\u003cp\u003eMycelium after staining treatment by fluorescence microscopy. (g, g-1): 0 μg/mL; (h, h-1): 50μg/mL; (k, k-1): 100 μg/mL; magnification: 40 × 10; scale for 10 μm.\u003c/p\u003e","description":"","filename":"6.png","url":"https://assets-eu.researchsquare.com/files/rs-6989432/v1/c32174a1f87e94cba157a874.png"},{"id":85940952,"identity":"0549f11e-d010-43f9-9089-79a2fb0b5583","added_by":"auto","created_at":"2025-07-03 11:41:03","extension":"png","order_by":7,"title":"Figure 7","display":"","copyAsset":false,"role":"figure","size":69864,"visible":true,"origin":"","legend":"\u003cp\u003eRelease of cellular contents from \u003cem\u003eP.c.\u003c/em\u003e after treatment with \u003cstrong\u003eY17 \u003c/strong\u003eDifferent letters indicate significant differences at p \u0026lt; 0.05 in the same group\u003c/p\u003e","description":"","filename":"7.png","url":"https://assets-eu.researchsquare.com/files/rs-6989432/v1/13e70aadc4eca107d36d6dc3.png"},{"id":85941010,"identity":"ce75c49b-0f2b-48c0-93b5-385b8b4925c6","added_by":"auto","created_at":"2025-07-03 11:41:07","extension":"png","order_by":8,"title":"Figure 8","display":"","copyAsset":false,"role":"figure","size":177705,"visible":true,"origin":"","legend":"\u003cp\u003eChanges in cell membrane permeability of \u003cstrong\u003eY17\u003c/strong\u003e against \u003cem\u003eP.c \u003c/em\u003eDifferent letters indicate significant differences at p \u0026lt; 0.05 in the same group\u003c/p\u003e","description":"","filename":"8.png","url":"https://assets-eu.researchsquare.com/files/rs-6989432/v1/6ea031bd51433a364ffa13da.png"},{"id":85940951,"identity":"c112e1e9-3564-407e-b0c6-a5b8b8ca5efd","added_by":"auto","created_at":"2025-07-03 11:41:03","extension":"png","order_by":9,"title":"Figure 9","display":"","copyAsset":false,"role":"figure","size":364404,"visible":true,"origin":"","legend":"\u003cp\u003eInhibition of \u003cem\u003eP.c\u003c/em\u003e by different concentrations of \u003cstrong\u003eY17\u003c/strong\u003e and dry weight of mycelium (Different letters indicate significant differences at p \u0026lt; 0.05 in the same group).\u003c/p\u003e","description":"","filename":"9.png","url":"https://assets-eu.researchsquare.com/files/rs-6989432/v1/92b7a1391e3dcd649596463b.png"},{"id":85940979,"identity":"ff6cb2b2-1e26-40e2-8f62-c185416e5d4c","added_by":"auto","created_at":"2025-07-03 11:41:04","extension":"png","order_by":10,"title":"Figure 10","display":"","copyAsset":false,"role":"figure","size":393791,"visible":true,"origin":"","legend":"\u003cp\u003eMDA content of \u003cem\u003eP.c\u003c/em\u003e after treatment with \u003cstrong\u003eY17\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eDifferent letters indicate significant differences at p \u0026lt; 0.05 in the same group\u003c/p\u003e","description":"","filename":"10.png","url":"https://assets-eu.researchsquare.com/files/rs-6989432/v1/3bb3615d675be9af4d7845a4.png"},{"id":85941441,"identity":"26fde10a-62a2-490e-afe0-8bf2332d3a1a","added_by":"auto","created_at":"2025-07-03 11:49:03","extension":"png","order_by":11,"title":"Figure 11","display":"","copyAsset":false,"role":"figure","size":1318820,"visible":true,"origin":"","legend":"\u003cp\u003eBiological safety of\u003cstrong\u003e Y17 (A)\u003c/strong\u003ePhotos of tobacco seeds soaked in \u003cstrong\u003eY17 \u003c/strong\u003esolution of different concentrations for 1, 4, 7, 10and 13 d. \u003cstrong\u003e(B)\u003c/strong\u003e Photos taken after 7 d of growth of seedlings sprayed with different concentrations of \u003cstrong\u003eY17 \u003c/strong\u003esolution\u003c/p\u003e","description":"","filename":"11.png","url":"https://assets-eu.researchsquare.com/files/rs-6989432/v1/658a53598b468ce69dacdd57.png"},{"id":85940931,"identity":"81d68949-df16-4923-badc-4573e97e0dc6","added_by":"auto","created_at":"2025-07-03 11:41:02","extension":"png","order_by":12,"title":"Figure 12","display":"","copyAsset":false,"role":"figure","size":648147,"visible":true,"origin":"","legend":"\u003cp\u003eLUMO/HOMO energy comparison of \u003cstrong\u003eY17\u003c/strong\u003e\u003c/p\u003e","description":"","filename":"12.png","url":"https://assets-eu.researchsquare.com/files/rs-6989432/v1/d487bf0dad7af44970b3bde8.png"},{"id":87335923,"identity":"d9b374dd-f415-428a-a7b1-4a3bb8771438","added_by":"auto","created_at":"2025-07-22 20:46:40","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":9050911,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-6989432/v1/aac9ba56-9517-4c0f-8a7e-4b2d063aff4c.pdf"},{"id":85940929,"identity":"dc555494-abd7-40b0-b630-a48240120069","added_by":"auto","created_at":"2025-07-03 11:41:02","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":4444133,"visible":true,"origin":"","legend":"","description":"","filename":"supporinformationY3.3.pdf","url":"https://assets-eu.researchsquare.com/files/rs-6989432/v1/980330403c0760094a92a5d4.pdf"},{"id":85940953,"identity":"e7b6efe5-1bbd-41f2-aa34-d8a2745cfe77","added_by":"auto","created_at":"2025-07-03 11:41:03","extension":"png","order_by":2,"title":"","display":"","copyAsset":false,"role":"supplement","size":1127405,"visible":true,"origin":"","legend":"","description":"","filename":"GraphicalAbstract.png","url":"https://assets-eu.researchsquare.com/files/rs-6989432/v1/5049d07bc06e75dec2f14f56.png"},{"id":85941009,"identity":"da091618-4466-48cc-ad74-20ee54e167ac","added_by":"auto","created_at":"2025-07-03 11:41:06","extension":"png","order_by":3,"title":"","display":"","copyAsset":false,"role":"supplement","size":424263,"visible":true,"origin":"","legend":"\u003cp\u003e\u003cstrong\u003eScheme 1\u003c/strong\u003e Schematic representation of the synthesis of target compounds \u003cstrong\u003eY1\u003c/strong\u003e–\u003cstrong\u003eY21\u003c/strong\u003e\u003c/p\u003e","description":"","filename":"Scheme1.png","url":"https://assets-eu.researchsquare.com/files/rs-6989432/v1/e1cb6ed12188810f76ae9ed2.png"}],"financialInterests":"No competing interests reported.","formattedTitle":"\u003cp\u003eDesign, synthesis and bioactivity studies of flavonol bio-containing \u003cem\u003eβ\u003c/em\u003e-amino alcohols\u003c/p\u003e","fulltext":[{"header":"1 Introduction","content":"\u003cp\u003ePlant diseases are a major factor constraining agricultural production and development, significantly reducing crop yield and quality, and causing severe economic losses [\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e, \u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e]. The primary pathogenic agents of plant diseases include microorganisms such as fungi [\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e], bacteria [\u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e], and viruses [\u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e, \u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e]. Among them, \u003cem\u003eP.c\u003c/em\u003e, a highly destructive plant pathogenic oomycete [\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e], can infect a wide range of economically important crops, including Solanaceae (capsicum annuum, solanum lycopersicum [\u003cspan citationid=\"CR8\" class=\"CitationRef\"\u003e8\u003c/span\u003e], solanum tuberosum, solanum melongena) and Cucurbitaceae [\u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e9\u003c/span\u003e] (Cucumis sativus, Cucurbita moschata, Citrullus lanatus, Cucumis melo, Cucurbita pepo) species. Currently, chemical fungicides remain the primary means of controlling plant diseases [\u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e]. However, the long-term and exclusive use of traditional fungicides can easily lead to pathogen resistance, increasing the difficulty of disease management [\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e]. Therefore, the development of low-toxicity, highly effective, and environmentally friendly novel pesticides has become a key research focus in the field of plant protection.\u003c/p\u003e \u003cp\u003eNatural products, also known as secondary metabolites, encompass a majority of chemical substances such as flavonoids, phenols, terpenoids, alkaloids, terpenes, and polysaccharides [\u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e12\u003c/span\u003e, \u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e13\u003c/span\u003e]. Among these, flavonol compounds are widely distributed in various plants [\u003cspan citationid=\"CR14\" class=\"CitationRef\"\u003e14\u003c/span\u003e] (ginkgo, onion, tea). Studies have shown that flavonoids have bacteriostatic activity [\u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e, \u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e], and this class of compounds exhibits varying degrees of inhibitory effects on a wide range of microorganisms, including gram-positive, gram-negative [\u003cspan citationid=\"CR17\" class=\"CitationRef\"\u003e17\u003c/span\u003e], and fungi [\u003cspan citationid=\"CR18\" class=\"CitationRef\"\u003e18\u003c/span\u003e]. Due to their remarkable antimicrobial efficacy, flavonol compounds hold significant research value and application potential. \u003cem\u003eβ\u003c/em\u003e-Amino alcohols are important fundamental chemical raw materials [\u003cspan citationid=\"CR19\" class=\"CitationRef\"\u003e19\u003c/span\u003e] with extensive applications across multiple fields, primarily including organic synthetic chemistry [\u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e20\u003c/span\u003e], pharmaceutical chemistry [\u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e21\u003c/span\u003e], natural product synthesis, and chemical production [\u003cspan citationid=\"CR22\" class=\"CitationRef\"\u003e22\u003c/span\u003e]. Moreover, \u003cem\u003eβ\u003c/em\u003e-amino alcohols exhibit wide-ranging applications in medicine [\u003cspan citationid=\"CR23\" class=\"CitationRef\"\u003e23\u003c/span\u003e], grinding aids [\u003cspan citationid=\"CR24\" class=\"CitationRef\"\u003e24\u003c/span\u003e], plasticizers, and surfactant. Many \u003cem\u003eβ\u003c/em\u003e-amino alcohol derivatives have attracted considerable attention due to their remarkable biological activities, including antiviral [\u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e25\u003c/span\u003e], antibacterial [\u003cspan citationid=\"CR26\" class=\"CitationRef\"\u003e26\u003c/span\u003e], antioxidant, anti-inflammatory, antiproliferative, and anticancer properties [\u003cspan citationid=\"CR27\" class=\"CitationRef\"\u003e27\u003c/span\u003e, \u003cspan citationid=\"CR28\" class=\"CitationRef\"\u003e28\u003c/span\u003e]. Based on the natural product flavonol as a lead compound, we designed and synthesized a series of flavonol derivatives containing \u003cem\u003eβ\u003c/em\u003e-amino alcohol motifs through a scaffold-hopping strategy (Fig.\u0026nbsp;\u003cspan refid=\"Fig10\" class=\"InternalRef\"\u003e1\u003c/span\u003e). The antifungal activity of all 21 synthesized compounds was evaluated against nine fungal strains. \u003cb\u003eY17\u003c/b\u003e exhibited the most potent inhibitory effect against \u003cem\u003eP.c\u003c/em\u003e, prompting further investigation into its antifungal mechanism of action.\u003c/p\u003e \u003cp\u003eFigure\u0026nbsp;\u003cspan refid=\"Fig10\" class=\"InternalRef\"\u003e1\u003c/span\u003e.\u003c/p\u003e"},{"header":"2 Results and Discussion","content":"\u003cdiv id=\"Sec3\" class=\"Section2\"\u003e \u003ch2\u003e2.1 \u003cem\u003eChemistry\u003c/em\u003e\u003c/h2\u003e \u003cp\u003eThe target compounds \u003cb\u003eY1-Y21\u003c/b\u003e were synthesized via a four-step reaction sequence, with the synthetic route illustrated in Scheme \u003cspan refid=\"Sch1\" class=\"InternalRef\"\u003e1\u003c/span\u003e. The structure of \u003cb\u003eY1\u003c/b\u003e-\u003cb\u003eY21\u003c/b\u003e was characterized using NMR and HRMS. Detailed physical characteristics and spectral data are provided in the \u003cb\u003esupporting information.\u003c/b\u003e\u003c/p\u003e \u003cp\u003eScheme \u003cspan refid=\"Sch1\" class=\"InternalRef\"\u003e1\u003c/span\u003e\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec4\" class=\"Section2\"\u003e \u003ch2\u003e2.2 Analysis of antifungal activity\u003c/h2\u003e \u003cdiv id=\"Sec5\" class=\"Section3\"\u003e \u003ch2\u003e2.2.1 Antifungal activity analysis \u003cem\u003ein vitro\u003c/em\u003e\u003c/h2\u003e \u003cp\u003eThe antibacterial activity of \u003cb\u003eY1\u003c/b\u003e-\u003cb\u003eY21\u003c/b\u003e against 9 plant fungi was measured at 100 \u0026micro;g/mL. From Table\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e, the experimental results demonstrated that several compounds exhibited significant antifungal activity at a concentration of 100 \u0026micro;g/mL. Notably, compounds \u003cb\u003eY11\u003c/b\u003e, \u003cb\u003eY12\u003c/b\u003e, \u003cb\u003eY15\u003c/b\u003e, \u003cb\u003eY16\u003c/b\u003e, \u003cb\u003eY17\u003c/b\u003e, \u003cb\u003eY18\u003c/b\u003e, \u003cb\u003eY21\u003c/b\u003eexhibited\u0026thinsp;\u0026gt;\u0026thinsp;90% inhibition rates against \u003cem\u003eP.c\u003c/em\u003e outperforming \u003cb\u003eAz\u003c/b\u003e (48.9%), compounds \u003cb\u003eY11\u003c/b\u003e, \u003cb\u003eY12\u003c/b\u003e, \u003cb\u003eY16\u003c/b\u003e, \u003cb\u003eY17\u003c/b\u003e, \u003cb\u003eY19\u003c/b\u003e and \u003cb\u003eY20\u003c/b\u003e exhibited\u0026thinsp;\u0026gt;\u0026thinsp;90% inhibition rates against \u003cem\u003eS.s\u003c/em\u003e, outperforming \u003cb\u003eAz\u003c/b\u003e (88.8%), compounds \u003cb\u003eY12\u003c/b\u003e, \u003cb\u003eY17\u003c/b\u003e, \u003cb\u003eY18\u003c/b\u003e, \u003cb\u003eY19\u003c/b\u003e and \u003cb\u003eY20\u003c/b\u003e exhibited\u0026thinsp;\u0026gt;\u0026thinsp;90% inhibition rates against \u003cem\u003eP.s\u003c/em\u003e, outperforming \u003cb\u003eAz\u003c/b\u003e (60.6%), the inhibition rates of \u003cb\u003eY15\u003c/b\u003e, \u003cb\u003eY17\u003c/b\u003e, and \u003cb\u003eY21\u003c/b\u003e on \u003cem\u003eBotryosphaeria dothidea (B.d)\u003c/em\u003e were 75.9, 82.4 and 74.3%, which were superior than \u003cb\u003eAz\u003c/b\u003e (69.1%), the inhibition rates of \u003cb\u003eY20\u003c/b\u003e on \u003cem\u003eFusarium graminearum (F.g)\u003c/em\u003e were 74.4%, which were superior than \u003cb\u003eAz\u003c/b\u003e (72.7%), To further investigate the antifungal potential of these compounds, the EC\u003csub\u003e50\u003c/sub\u003e values of the most active derivatives were determined according to published methodologies, with detailed results presented in Table\u0026nbsp;\u003cspan refid=\"Tab2\" class=\"InternalRef\"\u003e2\u003c/span\u003e. According to Table\u0026nbsp;\u003cspan refid=\"Tab2\" class=\"InternalRef\"\u003e2\u003c/span\u003e, the EC\u003csub\u003e50\u003c/sub\u003e of compounds \u003cb\u003eY17\u003c/b\u003e and \u003cb\u003eY21\u003c/b\u003e against \u003cem\u003eP.c\u003c/em\u003e were 5.2, 7.6 \u0026micro;g/mL, respectively, which were superior to that of \u003cb\u003eAz\u003c/b\u003e (98.5 \u0026micro;g/mL), and those of \u003cb\u003eY12\u003c/b\u003e and \u003cb\u003eY20\u003c/b\u003e against \u003cem\u003eS.s\u003c/em\u003e were 13.4 and 10.5 \u0026micro;g/mL, respectively, which were superior to that of \u003cb\u003eAz\u003c/b\u003e (50.1 \u0026micro;g/mL). Among them, \u003cb\u003eY17\u003c/b\u003e had the best EC\u003csub\u003e50\u003c/sub\u003e value, and the antifungal effect was the most significant. the growth of \u003cem\u003eP.c\u003c/em\u003e at different concentrations of \u003cb\u003eY17\u003c/b\u003e (0, 3.125, 6.25, 25, and 50 \u0026micro;g/mL) and \u003cb\u003eAz\u003c/b\u003e (0, 50, 100, 150, and 200 \u0026micro;g/mL) is shown in Fig.\u0026nbsp;\u003cspan refid=\"Fig11\" class=\"InternalRef\"\u003e2\u003c/span\u003e.\u003c/p\u003e \u003cp\u003eTable\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e.\u003c/p\u003e \u003cp\u003eTable\u0026nbsp;\u003cspan refid=\"Tab2\" class=\"InternalRef\"\u003e2\u003c/span\u003e.\u003c/p\u003e \u003cp\u003eFigure\u0026nbsp;\u003cspan refid=\"Fig11\" class=\"InternalRef\"\u003e2\u003c/span\u003e.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec6\" class=\"Section3\"\u003e \u003ch2\u003e2.2.2 Structure\u0026thinsp;\u0026minus;\u0026thinsp;activity relationship analysis.\u003c/h2\u003e \u003cp\u003eTo further investigate the effect of \u003cb\u003eR\u003c/b\u003e\u003csub\u003e\u003cb\u003e1\u003c/b\u003e\u003c/sub\u003e, \u003cb\u003eR\u003c/b\u003e\u003csub\u003e\u003cb\u003e2\u003c/b\u003e\u003c/sub\u003e and \u003cb\u003eR\u003c/b\u003e\u003csub\u003e\u003cb\u003e3\u003c/b\u003e\u003c/sub\u003e substituents on the inhibitory activity of the compounds, their structure-activity relationships were synthesized. As shown in Table\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e, Y1, Y2, Y3, Y4, and Y6 all showed less than 30% inhibition of \u003cem\u003eP.c\u003c/em\u003e, we can speculate that when \u003cb\u003eR\u003c/b\u003e\u003csub\u003e\u003cb\u003e3\u003c/b\u003e\u003c/sub\u003e is the same, changes in the substituents \u003cb\u003eR\u003c/b\u003e\u003csub\u003e\u003cb\u003e1\u003c/b\u003e\u003c/sub\u003e and \u003cb\u003eR\u003c/b\u003e\u003csub\u003e\u003cb\u003e2\u003c/b\u003e\u003c/sub\u003e of the compounds do not have much effect on the fungicidal activity. However, after replacing \u003cb\u003eR\u003c/b\u003e\u003csub\u003e\u003cb\u003e3\u003c/b\u003e\u003c/sub\u003e from unsaturated to saturated hydrocarbon group, the inhibitory activity of the compounds is significantly enhanced. Among them, \u003cb\u003eY11\u003c/b\u003e (96.7), \u003cb\u003eY12\u003c/b\u003e (90.5), \u003cb\u003eY15\u003c/b\u003e (93.5), \u003cb\u003eY16\u003c/b\u003e (92.6), \u003cb\u003eY17\u003c/b\u003e (100), \u003cb\u003eY18\u003c/b\u003e (97.1), \u003cb\u003eY19\u003c/b\u003e (91.3) and \u003cb\u003eY21\u003c/b\u003e (92.6%) showed more than 90% of fungicidal activity. Notably, although the fungicidal activity of \u003cb\u003eY5\u003c/b\u003e (28.1%) and \u003cb\u003eY13\u003c/b\u003e (53.7%) with the introduction of short-chain saturated hydrocarbon groups was still lower than 60%, the overall experimental data indicated that the introduction of saturated hydrocarbon groups could improve the fungicidal activity of the compounds.\u003c/p\u003e \u003c/div\u003e \u003c/div\u003e \u003cdiv id=\"Sec7\" class=\"Section2\"\u003e \u003ch2\u003e2.3 Antifungal activity of \u003cb\u003eY17\u003c/b\u003e against \u003cem\u003eP.c in vivo\u003c/em\u003e\u003c/h2\u003e \u003cp\u003eIn this experiment, \u003cb\u003eY17\u003c/b\u003e was selected for in vivo antifungal activity assay on eggplant fruits. As shown in Table\u0026nbsp;\u003cspan refid=\"Tab3\" class=\"InternalRef\"\u003e3\u003c/span\u003e and Fig.\u0026nbsp;\u003cspan refid=\"Fig12\" class=\"InternalRef\"\u003e3\u003c/span\u003e the range of eggplant fruit spots affected by \u003cb\u003eY17\u003c/b\u003e was significantly smaller than that of the control agent \u003cb\u003eAz\u003c/b\u003e, indicating that \u003cb\u003eY17\u003c/b\u003e had good protective and curative effects. At a concentration of 200 \u0026micro;g/mL, \u003cb\u003eY17\u003c/b\u003e had strong protective and curative effects on eggplant (85.7 and 77.8%, respectively), which was superior to \u003cb\u003eAz\u003c/b\u003e (42.9 and 35.5%). The curative and protective effects of \u003cb\u003eY17\u003c/b\u003e on eggplant at a concentration of 100 \u0026micro;g/mL were also stronger than those of the control agent. The results showed that \u003cb\u003eY17\u003c/b\u003e had good antifungal activity on eggplant fruits in vivo and could effectively control fungal diseases of eggplant crops.\u003c/p\u003e \u003cp\u003eTable\u0026nbsp;\u003cspan refid=\"Tab3\" class=\"InternalRef\"\u003e3\u003c/span\u003e.\u003c/p\u003e \u003cp\u003eFigure\u0026nbsp;\u003cspan refid=\"Fig12\" class=\"InternalRef\"\u003e3\u003c/span\u003e.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec8\" class=\"Section2\"\u003e \u003ch2\u003e2.4 SEM mycelial morphology analysis\u003c/h2\u003e \u003cp\u003eThe effect of \u003cb\u003eY17\u003c/b\u003e on the mycelial morphology of \u003cem\u003eP.c\u003c/em\u003e was further investigated using SEM, and the results were consistent with the conclusions of the pre-fungal inhibitory activity test, which showed that the mycelial morphology of mycosphaerella treated with \u003cb\u003eY17\u003c/b\u003e was significantly changed. As shown in Fig.\u0026nbsp;\u003cspan refid=\"Fig13\" class=\"InternalRef\"\u003e4\u003c/span\u003e, the mycelium of the undrug-treated control group had a full morphology with a smooth and uniform surface. When the concentration of \u003cb\u003eY17\u003c/b\u003e was 12.5 \u0026micro;g/mL, the mycelium produced folds, a few mycelia were bent, and growth was inhibited. When the concentration of \u003cb\u003eY17\u003c/b\u003e was increased to 25 \u0026micro;g/mL, most of the mycelium surface appeared serious shrinkage, wrinkles, mycelium became flattened and bent, and the growth status was obviously poor. In summary, \u003cb\u003eY17\u003c/b\u003e could destroy the mycelial morphology of \u003cem\u003eP.c\u003c/em\u003e mycelium and inhibit its normal growth. And the degree of mycelium destruction was intensifying as the concentration increased.\u003c/p\u003e \u003cp\u003eFigure\u0026nbsp;\u003cspan refid=\"Fig13\" class=\"InternalRef\"\u003e4\u003c/span\u003e.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec9\" class=\"Section2\"\u003e \u003ch2\u003e2.5 Fluorescence microscope (FM) experiments\u003c/h2\u003e \u003cp\u003ePropidium iodide (PI) is a nuclear DNA stain that does not pass through living cell membranes, but can cross broken cell membranes to stain the nucleus, and is commonly used for apoptosis detection. As shown in Fig.\u0026nbsp;\u003cspan refid=\"Fig14\" class=\"InternalRef\"\u003e5\u003c/span\u003e, after different concentrations of \u003cb\u003eY17\u003c/b\u003e (100 and 50 \u0026micro;g/mL) treated mycelium and stained with PI, the morphology of mycelium as well as the fluorescence effect was observed under fluorescence electron microscope, the results showed that the morphology of undrugged mycelium was intact and full, and there was no red fluorescence after staining, and the mycelium was destroyed and the red fluorescence became more and more obvious with the increasing concentration of \u003cb\u003eY17\u003c/b\u003e, which indicated that the destruction of the cell membrane of \u003cem\u003eP.c\u003c/em\u003e cells by the elevated concentration of the drug was intensifying and causing cell death.\u003c/p\u003e \u003cp\u003eFigure\u0026nbsp;\u003cspan refid=\"Fig14\" class=\"InternalRef\"\u003e5\u003c/span\u003e\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec10\" class=\"Section2\"\u003e \u003ch2\u003e2.6 ROS accumulation\u003c/h2\u003e \u003cp\u003eROS is a by-product of biological aerobic metabolism, when the level of ROS increases dramatically, it will cause an imbalance of the body's oxidation-antioxidant action, oxidative stress, causing lipid peroxidation, and ultimately lead to cell death. Among them, DCFH-DA fluorescent probe itself has no fluorescence, but it can freely pass through the cell membrane, and once it enters the cell, it can be hydrolyzed by intracellular esterase to generate non-fluorescent 2,7-dichlorodihydrofluorescein (DCFH), which is a fluorescence-less fluorescence. Once inside the cell, it can be hydrolyzed by intracellular esterase to form DCFH, and then DCFH will be oxidized by intracellular ROS to form a strong fluorescent product 2,7-dichlorofluorescein (DCF). Therefore, the level of ROS in the mycelial cells of \u003cem\u003eP.c\u003c/em\u003e was determined by using DCFH-DA as an indicator of oxidative stress [\u003cspan citationid=\"CR28\" class=\"CitationRef\"\u003e28\u003c/span\u003e]. As show in Fig.\u0026nbsp;\u003cspan refid=\"Fig15\" class=\"InternalRef\"\u003e6\u003c/span\u003e, the blank control group showed almost no green fluorescence, whereas the fluorescence intensity of \u003cem\u003eP.c\u003c/em\u003e mycelium treated with different concentrations of \u003cb\u003eY17\u003c/b\u003e increased with the concentration of \u003cb\u003eY17\u003c/b\u003e, further indicating that \u003cb\u003eY17\u003c/b\u003e can induce ROS production and cause membrane lipid peroxidation oxidation.\u003c/p\u003e \u003cp\u003eFigure\u0026nbsp;\u003cspan refid=\"Fig15\" class=\"InternalRef\"\u003e6\u003c/span\u003e.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec11\" class=\"Section2\"\u003e \u003ch2\u003e2.7 Effect on cytoplasmic leakage\u003c/h2\u003e \u003cp\u003eCells contain many macromolecules, and rupture of the cell membrane releases proteins, nucleic acids, and other macromolecules. The macromolecular leakage content of cell contents can be determined by measuring the absorbance of mycelial suspensions at 260 and 280 nm. From Fig.\u0026nbsp;\u003cspan refid=\"Fig17\" class=\"InternalRef\"\u003e8\u003c/span\u003e, it can be clearly seen that the absorbance value of the mycelial suspension treated with \u003cb\u003eY17\u003c/b\u003e increased significantly compared with the control group, \u003cb\u003eY17\u003c/b\u003e and with the increase in the concentration of \u003cb\u003eY17\u003c/b\u003e solution absorbance also increased, which shows that the \u003cb\u003eY17\u003c/b\u003e treatment can increase the amount of leakage of cytoplasmic contents, causing cell membrane damage, and the degree of damage increases with the increase in concentration.\u003c/p\u003e \u003cp\u003eFigure\u0026nbsp;\u003cspan refid=\"Fig16\" class=\"InternalRef\"\u003e7\u003c/span\u003e.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec12\" class=\"Section2\"\u003e \u003ch2\u003e2.8 Exploration of the determination of cell membrane permeability\u003c/h2\u003e \u003cp\u003eThe cell membrane serves as an important barrier for communication between the cell and the external environment, providing a relatively stable internal environment for the cell's life activities, and plays an important role in the normal life activities of the cell. When the cell membrane is damaged, intracellular electrolytes will extravasate and the relative conductivity will become larger. The relative conductivity changes of mycelium treated with different concentrations of \u003cb\u003eY17\u003c/b\u003e (0, 12.5, 25, 50, 100 \u0026micro;g/mL) are shown in Fig.\u0026nbsp;\u003cspan refid=\"Fig18\" class=\"InternalRef\"\u003e9\u003c/span\u003e. The results showed that, after 1 h of dosing, the relative conductivity value of \u003cb\u003eY17\u003c/b\u003e concentration of 100 \u0026micro;g/mL (85.7%) was 3.2 times higher than that of the blank group (26.3%), and the relative conductivity value of \u003cb\u003eY17\u003c/b\u003e concentration of 12.5 \u0026micro;g/mL (62.6%) was 2.3 times higher than that of the blank group (26.3%), and the relative conductivity became larger with the increase of \u003cb\u003eY17\u003c/b\u003e concentration. When the action time of \u003cb\u003eY17\u003c/b\u003e was between 1\u0026ndash;3 hours, the conductivity showed a more obvious upward trend and increased proportionally with the drug concentration and action time. These results suggest that \u003cb\u003eY17\u003c/b\u003e can destroy mycelial cell membranes and increase cell membrane permeability, thus leading to fungal death.\u003c/p\u003e \u003cp\u003eFigure\u0026nbsp;\u003cspan refid=\"Fig17\" class=\"InternalRef\"\u003e8\u003c/span\u003e.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec13\" class=\"Section2\"\u003e \u003ch2\u003e2.9 Cell dry weight experiment\u003c/h2\u003e \u003cp\u003eMycelial dry weight measurements were used to assess mycelial growth, and the experimental results were consistent with previous in vitro antifungal activity tests. As shown in Fig.\u0026nbsp;\u003cspan refid=\"Fig19\" class=\"InternalRef\"\u003e10\u003c/span\u003e, the dry weight of mycelium gradually decreased and the antifungal activity was enhanced as the concentration of \u003cb\u003eY17\u003c/b\u003e increased. The concentration of \u003cb\u003eY17\u003c/b\u003e was directly proportional to the antifungal activity and inversely proportional to the dry weight of mycelium. It can be seen that \u003cb\u003eY17\u003c/b\u003e can inhibit the normal growth of mycelium.\u003c/p\u003e \u003cp\u003eFigure\u0026nbsp;\u003cspan refid=\"Fig18\" class=\"InternalRef\"\u003e9\u003c/span\u003e.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec14\" class=\"Section2\"\u003e \u003ch2\u003e2.10 Determination of the MDA content\u003c/h2\u003e \u003cp\u003eMDA is one of the end products of lipid peroxidation and is a major indicator of monitoring cell membrane permeability, and its production can exacerbate cell membrane damage. Malondialdehyde yield was measured using malondialdehyde kit to detect the effect of \u003cb\u003eY17\u003c/b\u003e on cell membrane, and the results were shown in Fig.\u0026nbsp;\u003cspan refid=\"Fig20\" class=\"InternalRef\"\u003e11\u003c/span\u003e, which showed that the malondialdehyde content of \u003cb\u003eY17\u003c/b\u003e concentration of 100, 50, 25, and 12.5 \u0026micro;g/mL were increased by 1.88, 1.72, 1.44, and 1.37 times, respectively, in a dose-dependent manner, compared with that of the control group. The above results indicated that the drug could disrupt the cell membrane of \u003cem\u003eP.c\u003c/em\u003e leading to cell membrane damage.\u003c/p\u003e \u003cp\u003eFigure\u0026nbsp;\u003cspan refid=\"Fig19\" class=\"InternalRef\"\u003e10\u003c/span\u003e.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec15\" class=\"Section2\"\u003e \u003ch2\u003e2.11 Safety analysis\u003c/h2\u003e \u003cp\u003eAs shown in Fig.\u0026nbsp;\u003cspan refid=\"Fig9\" class=\"InternalRef\"\u003e12\u003c/span\u003e, spraying \u003cb\u003eY17\u003c/b\u003e at concentrations of 100, 200 and 400 \u0026micro;g/mL and equal doses of DMSO on chili pepper seeds was found to have almost no effect on the germination of chili pepper seeds. Similarly, spraying concentrations of 100, 200 and 400 \u0026micro;g/mL of \u003cb\u003eY17\u003c/b\u003e and equal doses of DMSO on chili peppers with the same growth cycle had almost no effect on the growth of chili peppers. It indicates that \u003cb\u003eY17\u003c/b\u003e has no significant effect on the germination and growth of chili pepper seeds and has good biosafety.\u003c/p\u003e \u003cp\u003eFigure\u0026nbsp;\u003cspan refid=\"Fig20\" class=\"InternalRef\"\u003e11\u003c/span\u003e.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec16\" class=\"Section2\"\u003e \u003ch2\u003e2.12 Energy calculation\u003c/h2\u003e \u003cp\u003eFrom the Table\u0026nbsp;\u003cspan refid=\"Tab4\" class=\"InternalRef\"\u003e4\u003c/span\u003e, it can be concluded that \u003cb\u003eAz\u003c/b\u003e and \u003cb\u003eY17\u003c/b\u003e have similar molecular weights and number of benzene rings, and both have similar core aromatic skeletons, which gives them similar membrane permeability and implies that they have similar solubility in solvents. \u003cem\u003eE\u003c/em\u003etotal: \u003cb\u003eAz\u003c/b\u003e (-1389.0443 Eh) is comparable to \u003cb\u003eY1\u003c/b\u003e7 (-1327.1366 Eh), indicating that they have similar stability.\u003c/p\u003e \u003cp\u003e \u003cb\u003eAz\u003c/b\u003e and \u003cb\u003eY17\u003c/b\u003e differed in electronic structure and reactivity. Activation energy (\u003cem\u003eEa\u003c/em\u003e): the energy barrier of \u003cb\u003eAz\u003c/b\u003e (0.1728 eV) is higher than that of \u003cb\u003eY17\u003c/b\u003e (0.1448 eV), which indicates that \u003cb\u003eY17\u003c/b\u003e is more reactive and kinetically more susceptible to reactions. The HOMO-LUMO energy gap (Δ\u003cem\u003eE\u003c/em\u003e): the energy barrier of \u003cb\u003eAz\u003c/b\u003e (0.1728 eV) is greater than that of \u003cb\u003eY17\u003c/b\u003e (0.1448 eV), which means that \u003cb\u003eY17\u003c/b\u003e is more susceptible to electronic transitions (electron transport). Susceptible to electron leaps (UV absorption may be red-shifted); more chemically reactive (front orbitals are more likely to be involved in reactions). Electronegativity (\u003cem\u003eχ\u003c/em\u003e): The electronegativity of \u003cb\u003eY17\u003c/b\u003e (4.0817 eV) is higher than that of \u003cb\u003eAz\u003c/b\u003e (3.8150 eV), suggesting that it is more capable of attracting electrons and may be more likely to participate in polar reactions (nucleophilic attack). The chemical potential (\u003cem\u003e\u0026micro;\u003c/em\u003e = -\u003cem\u003eχ\u003c/em\u003e) is lower (-4.0817 eV), suggesting that \u003cb\u003eY17\u003c/b\u003e is more thermodynamically stable. Electrophilicity index (\u003cem\u003eω\u003c/em\u003e): \u003cb\u003eY17\u003c/b\u003e (4.2283 eV) is significantly higher than \u003cb\u003eAz\u003c/b\u003e (3.0953 eV), suggesting that it is more capable of acting as an electron acceptor and may bind more efficiently to target proteins (bactericidal activity). Hardness (\u003cem\u003eη\u003c/em\u003e) vs. Softness (\u003cem\u003eS\u003c/em\u003e) Hardness: \u003cb\u003eAz\u003c/b\u003e (2.3511 eV)\u0026thinsp;\u0026gt;\u0026thinsp;\u003cb\u003eY17\u003c/b\u003e (1.9701 eV) \u003cb\u003eAz\u003c/b\u003e is \u0026ldquo;harder\u0026rdquo;, with a more difficult to polarize electronic structure, greater resistance to charge transfer, and greater spatial site resistance Softness: \u003cb\u003eY17\u003c/b\u003e (0.2538 eV\u003csup\u003e\u0026minus;\u003c/sup\u003e\u0026sup1;) higher, more susceptible to charge transfer, suitable for catalytic or coordination reactions.\u003c/p\u003e \u003cp\u003eTable\u0026nbsp;\u003cspan refid=\"Tab4\" class=\"InternalRef\"\u003e4\u003c/span\u003e.\u003c/p\u003e \u003cp\u003eWe investigated the biological activities and mechanisms using frontline molecular orbital theory. As shown in Fig.\u0026nbsp;\u003cspan refid=\"Fig9\" class=\"InternalRef\"\u003e12\u003c/span\u003e, the HOMO of \u003cb\u003eY17\u003c/b\u003e is mainly concentrated on the \u003cem\u003eβ\u003c/em\u003e-amino alcohol structure; the LUMO is mainly located on the backbone and ring of flavonols with an energy difference of 0.1448 eV. The electron transfer is from \u003cem\u003eβ\u003c/em\u003e-amino alcohol to chalcone ring. The HOMO of \u003cb\u003eAz\u003c/b\u003e is mainly located on the benzene ring close to the carbonyl group, on the methoxy and carbonyl structures, and the LUMO is mainly located on the cyano group and on the benzene ring attached to the cyano group, with an energy difference of 0.1728 eV. The electron transfer is from the benzene ring at one end to the benzene ring at the other end. From the above study, it is clear that the structure of current commercial drug \u003cb\u003eAz\u003c/b\u003e has electron migration phenomenon indicating that the structure with electron migration behavior may be biologically active. \u003cb\u003eY17\u003c/b\u003e both have electron migration phenomenon and lower energy difference value indicating that it has high reactivity.\u003c/p\u003e \u003cp\u003eFigure\u0026nbsp;\u003cspan refid=\"Fig9\" class=\"InternalRef\"\u003e12\u003c/span\u003e.\u003c/p\u003e \u003c/div\u003e"},{"header":"3 Conclusion","content":"\u003cp\u003eIn conclusion, twenty-one flavonol derivatives of \u003cem\u003eβ\u003c/em\u003e-amino alcohols were designed and synthesized. The target compound \u003cb\u003eY17\u003c/b\u003e exhibited superior antifungal activity against \u003cem\u003eP.c\u003c/em\u003e with EC\u003csub\u003e50\u003c/sub\u003e values of 5.2 \u0026micro;g/mL, respectively, which exceeded that of \u003cb\u003eAz\u003c/b\u003e (98.5 \u0026micro;g/mL, and at a concentration of 200 \u0026micro;g/mL, \u003cb\u003eY17\u003c/b\u003e showed significant protection in eggplant fruits (85.7%), which was superior to that of the \u003cb\u003eAz\u003c/b\u003e (42.9%). Scanning electron microscopy and fluorescence microscopy analyses of \u003cb\u003eY17\u003c/b\u003e-treated aspergillus oryzae mycelium of pepper revealed significant morphological changes, including mycelial shrinkage, structural deformation and membrane rupture. In addition, further studies showed that \u003cb\u003eY17\u003c/b\u003e was able to disrupt the integrity of the cell membrane of the pathogenic fungus, affecting its lipid peroxidation and leading to the release of intracellular solutes. Chemical studies through computational simulations such as molecular dynamics (MD). The experimental results further confirmed that flavonol ketone derivatives containing \u003cem\u003eβ\u003c/em\u003e-amino alcohol moiety are promising candidates for the development of novel fungicides.\u003c/p\u003e"},{"header":"4. Materials and methods","content":"\u003cdiv id=\"Sec19\" class=\"Section2\"\u003e \u003ch2\u003e4.1 Chemicals and Instruments\u003c/h2\u003e \u003cp\u003eProton nuclear magnetic resonance (NMR) spectra were obtained by using the JEOL-JMTC-600(Japan, Hyogo), JEOL-ECX500 (Tokyo, Japan) and Bruker-ASCEND400 (Karlsruhe, Germany) spectrometers operated at room temperature with CDCl\u003csub\u003e3\u003c/sub\u003e as the solvent and tetramethylsilane as the internal standard. HRMS was conducted using Thermo Scientific Q Exactive (Thermo Scientific, Missouri). The morphology of the fungal mycelia was examined using a Nova Nano SEM 450 instrument (Field Electron and Ion Co.), an Olympus-BX53 fluorescence microscope (Olympus, Japan). The cell permeability was measured on the conductivity meter, Leici DDSJ-3O8F (Shanghai Instrument \u0026amp; Electric Science Instrument Co., Ltd., Shanghai, China), and the cell leakage was recorded on the N-5000 ultraviolet spectrophotometer (Shanghai Yoke Instrument Co., Ltd., Shanghai, China). The MDA assay kit was purchased from Beijing Solarbio Science \u0026amp; Technology Co. The reagents and solvents used in the experiment were purchased from Bositai Technology Co., Ltd. (Chongqing, China) or Shanghai Titan Chemical Co., Ltd. (Shanghai, China). The melting point measurements were obtained by using an X-4B melting point instrument (Shanghai INESA Co., Ltd., Shanghai, China).\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec20\" class=\"Section2\"\u003e \u003ch2\u003e4.2 Synthesis of intermediates and target compounds\u003c/h2\u003e \u003cdiv id=\"Sec21\" class=\"Section3\"\u003e \u003ch2\u003e4.2.1 Synthesis of intermediates \u003cb\u003e1\u0026ndash;3\u003c/b\u003e\u003c/h2\u003e \u003cp\u003eReferences [\u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e25\u003c/span\u003e, \u003cspan citationid=\"CR29\" class=\"CitationRef\"\u003e29\u003c/span\u003e], substituted 2'-Hydroxyacetophenone(1.66 mmol) and substituted benzaldehyde (1.66 mmol) were put into a 500 mL round-bottomed flask, ethanol was used as solvent, and hydroxyl aldehyde condensation took place at room temperature and under alkaline conditions, and at the end of the reaction it was precipitated out in iced water (pH\u0026thinsp;=\u0026thinsp;4\u0026ndash;5) to obtain intermediate \u003cb\u003e1\u003c/b\u003e; in a 500 mL round-bottomed flask, intermediate \u003cb\u003e1\u003c/b\u003e (1 mmol) reacted with hydrogen peroxide under alkaline conditions to obtain intermediate \u003cb\u003e2\u003c/b\u003e; epoxy bromopropane (1.50 mmol) and intermediate \u003cb\u003e2\u003c/b\u003e (2.20 mmol) in DMF solution, and after TCL detection the reaction was completed. In a 500 mL round bottom flask, intermediate \u003cb\u003e1\u003c/b\u003e (1 mmol) and hydrogen peroxide under alkaline conditions, the ring-closing reaction to obtain intermediate \u003cb\u003e2\u003c/b\u003e; epoxybromopropane (1.50 mmol) and intermediate \u003cb\u003e2\u003c/b\u003e (2.20 mmol) in DMF solution, TCL detection reaction after the end of the extraction with dichloromethane, concentrated under reduced pressure to get the intermediate \u003cb\u003e3\u003c/b\u003e.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec22\" class=\"Section3\"\u003e \u003ch2\u003e4.2.2 Synthesis of the target compounds\u003c/h2\u003e \u003cp\u003eIntermediate \u003cb\u003e3\u003c/b\u003e (1 mmol), substituted ammonia (3 mmol), isopropanol as solvent and K\u003csub\u003e2\u003c/sub\u003eCO\u003csub\u003e3\u003c/sub\u003e (3 mmol) as acid-binding agent were put into a 100 mL round-bottomed flask and stirred at reflux at 80\u0026deg;C. After the completion of the reaction was monitored by TLC, the product was cooled down to room temperature, poured into iced water to precipitate, left to stand, filtered and dried [\u003cspan citationid=\"CR30\" class=\"CitationRef\"\u003e30\u003c/span\u003e]. The crude product was isolated and purified by column chromatography (methanol: dichloromethane\u0026thinsp;=\u0026thinsp;1:10, \u003cem\u003ev/v\u003c/em\u003e) to obtain the target compounds \u003cb\u003eY1-Y21\u003c/b\u003e.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec23\" class=\"Section3\"\u003e \u003ch2\u003e4.2.3 Synthesis of compounds \u003cb\u003eY1\u003c/b\u003e-\u003cb\u003eY21.\u003c/b\u003e\u003c/h2\u003e \u003cp\u003e3-(3-(benzylamino)-2-hydroxypropoxy)-2-phenyl-4\u003cem\u003eH\u003c/em\u003e-chromen-4-one(\u003cb\u003eY1\u003c/b\u003e). White solid; m.p. 124.9-126.5 ℃; Yield 56%; \u003csup\u003e1\u003c/sup\u003eH NMR (500 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ 8.27 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.0 Hz, 1H), 8.09 (m, 2H), 7.72 (m, 1H), 7.60\u0026ndash;7.50 (m, 4H), 7.44 (t, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;7.5 Hz, 1H), 7.14 (m, 2H), 6.65 (m, 3H), 5.33 (s, 1H), 4.31\u0026ndash;3.88 (m, 4H), 3.24 (m, 2H). \u003csup\u003e13\u003c/sup\u003eC NMR (125 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ 176.18, 157.07, 155.54, 148.46, 140.65, 134.20, 131.41, 130.56, 129.32, 128.96, 128.68, 126.05, 125.28, 123.73, 118.27, 117.62, 113.25, 76.69, 68.98, 45.99. HRMS(ESI) m/z [M\u0026thinsp;+\u0026thinsp;H]\u003csup\u003e+\u003c/sup\u003e Calcd for C\u003csub\u003e24\u003c/sub\u003eH\u003csub\u003e22\u003c/sub\u003eNO\u003csub\u003e4\u003c/sub\u003e 388.15433 found 388.15308.\u003c/p\u003e \u003cp\u003e2-(4-bromophenyl)-3-(2-hydroxy-3-(phenylamino)propoxy)-4\u003cem\u003eH\u003c/em\u003e-chromen-4-one (\u003cb\u003eY2\u003c/b\u003e). Orange oily; Yield 61%; \u003csup\u003e1\u003c/sup\u003eH NMR (500 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ 8.29\u0026ndash;8.23 (m, 1H), 8.00\u0026ndash;7.95 (m, 2H), 7.78\u0026ndash;7.58 (m, 4H), 7.50 (m, 2H), 7.14 (m, 2H), 6.68 (t, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;7.5 Hz, 1H), 6.62 (m, 2H), 4.17\u0026ndash;4.12 (m, 1H), 4.02 (m, 1H), 3.94 (m, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;10.8, 7.0 Hz, 1H), 3.31 (m, 1H), 3.23\u0026ndash;3.16 (m, 1H). \u003csup\u003e13\u003c/sup\u003eC NMR (125 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ 176.01, 155.82, 155.43, 148.41, 140.72, 134.33, 132.25, 130.14, 129.44, 129.34, 126.09, 126.07, 125.40, 123.72, 118.21, 117.72, 113.28, 76.56, 69.04, 46.03. HRMS(ESI) m/z [M\u0026thinsp;+\u0026thinsp;H]\u003csup\u003e+\u003c/sup\u003e Calcd for C\u003csub\u003e24\u003c/sub\u003eH\u003csub\u003e21\u003c/sub\u003eO\u003csub\u003e4\u003c/sub\u003eNBr 466.06485, found 466.06363.\u003c/p\u003e \u003cp\u003e3-(2-hydroxy-3-(phenylamino)propoxy)-2-(4-methoxyphenyl)-4\u003cem\u003eH\u003c/em\u003e-chromen-4-one(\u003cb\u003eY3\u003c/b\u003e). Yellow solid; m.p. 112.8-113.7℃; Yield 47%; \u003csup\u003e1\u003c/sup\u003eH NMR (500 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ 8.32\u0026ndash;8.02 (m, 3H), 7.75\u0026ndash;7.65 (m, 1H), 7.58\u0026ndash;7.39 (m, 2H), 7.28\u0026ndash;6.97 (m, 5H), 6.69\u0026ndash;6.59 (m, 2H), 5.43 (s, 1H), 4.33\u0026ndash;3.95 (m, 4H), 3.93\u0026ndash;3.82 (m, 3H), 3.24 (m, 2H). \u003csup\u003e13\u003c/sup\u003eC NMR (125 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ 175.96, 162.01, 157.03, 155.39, 148.50, 139.96, 133.94, 130.47, 129.31, 126.01, 125.12, 123.72, 122.78, 118.12, 117.59, 114.39, 113.25, 76.48, 69.02, 55.59, 46.02. HRMS(ESI) m/z [M\u0026thinsp;+\u0026thinsp;H]\u003csup\u003e+\u003c/sup\u003e Calcd for C\u003csub\u003e25\u003c/sub\u003eH\u003csub\u003e24\u003c/sub\u003eO\u003csub\u003e5\u003c/sub\u003eN 418.16490, found 418.16373.\u003c/p\u003e \u003cp\u003e2-(4-chlorophenyl)-3-(2-hydroxy-3-(phenylamino)propoxy)-4\u003cem\u003eH\u003c/em\u003e-chromen-4-one(\u003cb\u003eY4\u003c/b\u003e).Yellow solid; m.p. 141.1-142.5 ℃; Yield 51%; \u003csup\u003e1\u003c/sup\u003eH NMR (500 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ 8.30\u0026ndash;8.22 (m, 1H), 8.12\u0026ndash;7.98 (m, 2H), 7.72 (ddd, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;15, 8.0, 6.5 Hz, 1H), 7.49 (m, 4H), 7.14 (t, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;7.5 Hz, 2H), 6.66 (m, 3H), 5.55\u0026ndash;4.82 (m, 1H), 4.20\u0026ndash;4.11 (m, 1H), 3.97 (m, 2H), 3.37\u0026ndash;3.15 (m, 2H). \u003csup\u003e13\u003c/sup\u003eC NMR (126 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ 175.96, 162.01, 157.03, 155.39, 148.50, 139.96, 133.94, 130.47, 129.31, 126.01, 125.12, 123.72, 122.78, 118.12, 117.59, 114.39, 113.25, 76.48, 69.02, 55.59, 46.02. HRMS(ESI) m/z [M\u0026thinsp;+\u0026thinsp;H]\u003csup\u003e+\u003c/sup\u003e Calcd for C\u003csub\u003e24\u003c/sub\u003eH\u003csub\u003e21\u003c/sub\u003eO\u003csub\u003e4\u003c/sub\u003eNCl 422.11536, found 422.11398.\u003c/p\u003e \u003cp\u003e3-(3-(ethylamino)-2-hydroxypropoxy)-2-phenyl-4\u003cem\u003eH\u003c/em\u003e-chromen-4-one\u003cb\u003e(Y5)\u003c/b\u003e.Orange oily; Yield 47%; \u003csup\u003e1\u003c/sup\u003eH NMR (500 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ 8.15 (dd, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.0, 2.0 Hz, 1H), 8.04 (dd, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;7.5, 2.5 Hz, 2H), 7.69\u0026ndash;7.65 (m, 1H), 7.52\u0026ndash;7.48 (m, 4H), 7.37 (t, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;7.5 Hz, 1H), 5.09 (s, 2H), 4.21 (t, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;5.0 Hz, 1H), 3.94 (dd, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;10.5, 4.5 Hz, 1H), 3.88 (dd, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;10.5, 5.5 Hz, 1H), 3.03 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;5.5 Hz, 2H), 2.97\u0026ndash;2.84 (m, 2H), 1.28 (t, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;7.5 Hz, 3H). \u003csup\u003e13\u003c/sup\u003eC NMR (125 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ 176.06, 157.11, 155.45, 140.16, 134.24, 131.48, 130.24, 128.95, 128.67, 125.84, 125.26, 123.52, 118.24, 74.98, 67.29, 51.30, 44.16, 13.26. HRMS(ESI) m/z [M\u0026thinsp;+\u0026thinsp;H]\u003csup\u003e+\u003c/sup\u003e Calcd for C\u003csub\u003e20\u003c/sub\u003eH\u003csub\u003e22\u003c/sub\u003eO\u003csub\u003e4\u003c/sub\u003eN 340.15633, found 340.15403.\u003c/p\u003e \u003cp\u003e3-(3-(benzylamino)-2-hydroxypropoxy)-2-(p-tolyl)-4\u003cem\u003eH\u003c/em\u003e-chromen-4-one(\u003cb\u003eY6\u003c/b\u003e). Orange oily; Yield 57%; \u003csup\u003e1\u003c/sup\u003eH NMR (500 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ 8.26 (dd, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.0, 1.5 Hz, 1H), 8.00 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.5 Hz, 2H), 7.70 (m, 1H), 7.55 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.5 Hz, 1H), 7.43\u0026ndash;7.40 (m, 1H), 7.33 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.0 Hz, 2H), 7.18\u0026ndash;7.11 (m, 3H), 6.71\u0026ndash;6.60 (m, 4H), 4.20\u0026ndash;3.89 (m, 4H), 3.30 (dd, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;12.5, 5.0 Hz, 1H), 3.20 (dd, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;12.5, 6.5 Hz, 1H), 2.44 (s, 3H). \u003csup\u003e13\u003c/sup\u003eC NMR (125 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ 176.09, 157.28, 155.47, 148.51, 142.03, 140.38, 134.08, 129.71, 129.39, 129.33, 128.63, 127.69, 125.98, 125.19, 123.72, 118.24, 117.59, 113.27, 76.50, 69.00, 46.04, 29.86, 21.77. HRMS(ESI) m/z [M\u0026thinsp;+\u0026thinsp;H]\u003csup\u003e+\u003c/sup\u003e Calcd for C\u003csub\u003e25\u003c/sub\u003eH\u003csub\u003e24\u003c/sub\u003eO\u003csub\u003e4\u003c/sub\u003eN. 402.16998, found 402.16885.\u003c/p\u003e \u003cp\u003e3-(3-(benzylamino)-2-hydroxypropoxy)-2-phenyl-4\u003cem\u003eH\u003c/em\u003e-chromen-4-one(\u003cb\u003eY7\u003c/b\u003e). Yellow oily; Yield 53%; \u003csup\u003e1\u003c/sup\u003eH NMR \u003csup\u003e1\u003c/sup\u003eH NMR (500 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ 8.11\u0026ndash;7.91 (m, 4H), 7.42 (m, 6H), 7.24 (m, 4H), 5.51 (s, 2H), 4.21 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;5.5 Hz, 1H), 4.05 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;6.5 Hz, 2H), 3.86\u0026ndash;3.82 (m, 1H), 3.01 (m, 1H), 2.82 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;36 Hz, 2H).\u003csup\u003e13\u003c/sup\u003eC NMR (125 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ 175.88, 162.82, 156.98, 155.36, 140.07, 134.45, 134.26, 131.48, 130.14, 129.66, 128.93, 128.84, 128.63, 125.72, 125.26, 123.44, 118.24, 74.83, 67.07, 52.75, 50.59. HRMS(ESI) m/z [M\u0026thinsp;+\u0026thinsp;H]\u003csup\u003e+\u003c/sup\u003e Calcd for C\u003csub\u003e25\u003c/sub\u003eH\u003csub\u003e24\u003c/sub\u003eO\u003csub\u003e4\u003c/sub\u003eN. 402.16998, found 402.16879\u003c/p\u003e \u003cp\u003e2-(4-(tert-butyl)phenyl)-3-(2-hydroxy-3-(phenylamino)propoxy)-4\u003cem\u003eH\u003c/em\u003e-chromen-4-one(\u003cb\u003eY8\u003c/b\u003e). Yellow oily; Yield 42%; \u003csup\u003e1\u003c/sup\u003eH NMR (400 MHz, CDCl3) δ 8.29 (dd, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.0, 1.6 Hz, 1H), 8.06 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.8 Hz, 2H), 7.75\u0026ndash;7.70 (m, 1H), 7.56 (dd, J\u0026thinsp;=\u0026thinsp;8.4,6.0 Hz, 3H), 7.45 (t, J\u0026thinsp;=\u0026thinsp;7.6 Hz, 1H), 7.16 (dd, J\u0026thinsp;=\u0026thinsp;8.4, 7.2 Hz, 2H), 6.67 (m, 3H), 5.41 (s, 1H), 4.33\u0026ndash;4.13 (m, 2H), 4.05 (dd, J\u0026thinsp;=\u0026thinsp;10.8, 2.6 Hz, 1H), 3.96 (dd, J\u0026thinsp;=\u0026thinsp;10.8, 7.2 Hz, 1H), 3.33 (dd, J\u0026thinsp;=\u0026thinsp;12.4, 4.8 Hz, 1H), 3.21 (dd, J\u0026thinsp;=\u0026thinsp;12.4, 6.4 Hz, 1H), 1.38 (s, 9H). \u003csup\u003e13\u003c/sup\u003eC NMR (100 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ 176.07, 157.15, 155.43, 154.96, 148.40, 140.39, 133.98, 129.23, 128.36, 127.58, 125.97, 125.89, 125.11, 123.66, 118.13, 117.53, 113.16, 76.62, 68.94, 45.91, 35.09, 31.16. HRMS(ESI) m/z [M\u0026thinsp;+\u0026thinsp;H]\u003csup\u003e+\u003c/sup\u003e Calcd for C\u003csub\u003e28\u003c/sub\u003eH\u003csub\u003e30\u003c/sub\u003eO\u003csub\u003e4\u003c/sub\u003eN. 444.21693, found 444.21591.\u003c/p\u003e \u003cp\u003e3-(3-(benzylamino)-2-hydroxypropoxy)-2-(p-tolyl)-4\u003cem\u003eH\u003c/em\u003e-chromen-4-one\u003cb\u003e(Y9)\u003c/b\u003e. White solid; m.p. 139.9-141.6℃; Yield 57%; \u003csup\u003e1\u003c/sup\u003eH NMR \u003csup\u003e1\u003c/sup\u003eH NMR (400 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ 8.25 (dd, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.0, 1.6 Hz, 1H), 8.00 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.4 Hz, 2H), 7.69 (m, 1H), 7.54 (m, 1H), 7.43\u0026ndash;7.39 (m, 1H), 7.36\u0026ndash;7.26 (m, 7H), 4.57\u0026ndash;4.25 (m, 1H), 4.07\u0026ndash;3.95 (m, 2H), 3.85 (dd, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;10.8, 7.6 Hz, 1H), 3.78 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;1.6 Hz, 2H), 3.53\u0026ndash;3.27 (m, 1H), 2.72 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;5.6 Hz, 2H), 2.44 (s, 3H). \u003csup\u003e13\u003c/sup\u003eC NMR (100 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ 175.90, 156.97, 155.36, 141.77, 140.30, 140.12, 133.83, 129.53, 128.86, 128.52, 128.38, 128.19, 128.08, 127.71, 126.94, 125.90, 124.98, 123.72, 118.09, 76.61, 69.19, 53.98, 51.13, 21.64. HRMS(ESI) m/z [M\u0026thinsp;+\u0026thinsp;H]\u003csup\u003e+\u003c/sup\u003e Calcd for C\u003csub\u003e26\u003c/sub\u003eH\u003csub\u003e26\u003c/sub\u003eO\u003csub\u003e4\u003c/sub\u003eN. 416.18563, found 416.18393.\u003c/p\u003e \u003cp\u003e3-(3-(benzylamino)-2-hydroxypropoxy)-2-(4-chlorophenyl)-7-methoxy-4\u003cem\u003eH\u003c/em\u003e-chromen-4-one (\u003cb\u003eY10\u003c/b\u003e). brown solid; m.p. 103.2-104.1℃; Yield 48%;\u003csup\u003e1\u003c/sup\u003eH NMR (500 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ 8.14 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;9 Hz, 1H), 8.03\u0026ndash;8.01 (m, 2H), 7.49\u0026ndash;7.46 (m, 2H), 7.14 (dd, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.5, 7.5 Hz, 2H), 6.99 (dd, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;9.0, 2.5 Hz, 1H), 6.91 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;2.5 Hz, 1H), 6.68 (dd, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.0, 7 Hz, 1H), 6.63\u0026ndash;6.61 (m, 2H), 5.45 (s, 1H), 4.19\u0026ndash;4.09 (m, 2H), 4.01\u0026ndash;3.99 (m, 1H), 3.94 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;6.5 Hz, 2H), 3.92 (s, 3H), 3.89 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;5.5 Hz, 1H), 3.31\u0026ndash;3.27 (m, 1H), 3.21\u0026ndash;3.17 (m, 1H). \u003csup\u003e13\u003c/sup\u003eC NMR (126 MHz, CHLOROFORM-\u003cem\u003eD\u003c/em\u003e) δ 175.34, 164.71, 157.29, 155.16, 148.46, 140.52, 137.31, 129.82, 129.32, 129.22, 129.07, 127.39, 117.65, 117.50, 115.23, 113.26, 100.05, 76.62, 69.04, 56.07, 46.04. HRMS(ESI) m/z [M\u0026thinsp;+\u0026thinsp;H]\u003csup\u003e+\u003c/sup\u003e Calcd for C\u003csub\u003e26\u003c/sub\u003eH\u003csub\u003e25\u003c/sub\u003eO\u003csub\u003e5\u003c/sub\u003eNCl. 466.14158, found 466.14111.\u003c/p\u003e \u003cp\u003e3-(3-(benzylamino)-2-hydroxypropoxy)-2-(4-chlorophenyl)-4\u003cem\u003eH\u003c/em\u003e-chromen-4-one\u003cb\u003e(Y11)\u003c/b\u003e. Brown oily; Yield 35%;\u003csup\u003e1\u003c/sup\u003eH NMR (400 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ 8.24 (dd, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.1, 1.7 Hz, 1H), 8.07 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.8 Hz, 2H), 7.74\u0026ndash;7.70 (m, 1H), 7.49 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.7 Hz, 2H), 7.35\u0026ndash;7.28 (m, 7H), 4.61 (s, 1H), 4.05\u0026ndash;3.98 (m, 2H), 3.95\u0026ndash;3.84 (m, 2H), 3.78 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;1.5 Hz, 2H), 2.75\u0026ndash;2.69 (m, 2H).\u003csup\u003e13\u003c/sup\u003eC NMR (101 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ 175.80, 155.44, 155.29, 140.60, 139.98, 137.35, 134.10, 129.94, 129.10, 128.43, 128.19, 127.02, 125.95, 125.20, 123.68, 118.08, 76.50, 69.16, 53.94, 51.06.HRMS(ESI) m/z [M\u0026thinsp;+\u0026thinsp;H]\u003csup\u003e+\u003c/sup\u003e Calcd for C\u003csub\u003e25\u003c/sub\u003eH\u003csub\u003e23\u003c/sub\u003eO\u003csub\u003e4\u003c/sub\u003eNCl. 436.13319, found 436.12988.\u003c/p\u003e \u003cp\u003e3-(3-(hexylamino)-2-hydroxypropoxy)-2-phenyl-4\u003cem\u003eH\u003c/em\u003e-chromen-4-one\u003cb\u003e(Y12)\u003c/b\u003e. Brown oily; Yield 40%;\u003csup\u003e1\u003c/sup\u003eH NMR (600 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ 8.20 (dd, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.4, 1.8 Hz, 1H), 8.09\u0026ndash;8.05 (m, 2H), 7.69 (m, 1H), 7.55\u0026ndash;7.50 (m, 4H), 7.40 (m, 1H), 4.21 (s, 2H), 4.09 (m, 1H), 3.96 (dd, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;10.2, 3.6 Hz, 1H), 3.86 (dd, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;10.2, 6.6 Hz, 1H), 2.86 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;5.4 Hz, 2H), 2.74\u0026ndash;2.65 (m, 2H), 1.60\u0026ndash;1.51 (m, 2H), 1.30\u0026ndash;1.23 (m, 6H), 0.84 (t, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;6.6 Hz, 3H). \u003csup\u003e13\u003c/sup\u003eC NMR (150 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ 176.05, 156.97, 155.49, 140.40, 134.13, 131.37, 130.45, 128.90, 128.67, 125.94, 125.20, 123.68, 118.22, 75.80, 68.17, 51.75, 49.91, 31.69, 28.95, 26.90, 22.65, 14.12. HRMS(ESI) m/z [M\u0026thinsp;+\u0026thinsp;H]\u003csup\u003e+\u003c/sup\u003e Calcd for C\u003csub\u003e24\u003c/sub\u003eH\u003csub\u003e30\u003c/sub\u003eO\u003csub\u003e4\u003c/sub\u003eN. 396.21693, found 396.21567.\u003c/p\u003e \u003cp\u003e3-(3-(ethylamino)-2-hydroxypropoxy)-2-(4-fluorophenyl)-4\u003cem\u003eH\u003c/em\u003e-chromen-4-one(\u003cb\u003eY13\u003c/b\u003e). brown oily; Yield 47%; \u003csup\u003e1\u003c/sup\u003eH NMR (500 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ 8.07\u0026ndash;7.96 (m, 2H), 7.72 (dd, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.0, 3.0 Hz, 1H), 7.56\u0026ndash;7.45 (m, 4H), 7.40 (m, 1H), 4.37 (t, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;5.5 Hz, 1H), 4.00\u0026ndash;3.89 (m, 2H), 3.37\u0026ndash;3.16 (m, 4H), 1.45 (t, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;7.5 Hz, 3H). \u003csup\u003e13\u003c/sup\u003eC NMR (125 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ 175.17, 160.45, 158.48, 157.51, 151.74, 139.54, 131.78, 129.78, 129.06, 128.69, 124.65, 124.59, 122.81, 122.60, 120.50, 120.44, 110.49, 110.30, 73.70, 65.82, 50.91, 44.14, 11.46.\u003csup\u003e19\u003c/sup\u003eF NMR (565 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ -120.31. HRMS(ESI) m/z [M\u0026thinsp;+\u0026thinsp;H]\u003csup\u003e+\u003c/sup\u003e Calcd for C\u003csub\u003e20\u003c/sub\u003eH\u003csub\u003e21\u003c/sub\u003eFNO\u003csub\u003e4\u003c/sub\u003e. 358.14491, found 358.14377.\u003c/p\u003e \u003cp\u003e3-(3-((4-fluorobenzyl)-l2-azaneyl)-2-hydroxypropoxy)-2-phenyl-4\u003cem\u003eH\u003c/em\u003e-chromen-4-one(\u003cb\u003eY14\u003c/b\u003e). Brown oily; Yield 54%; \u003csup\u003e1\u003c/sup\u003eH NMR (500 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ 8.23 (dd, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.0, 2.0 Hz, 1H), 8.08 (m, 2H), 7.70 (m, 1H), 7.56\u0026ndash;7.50 (m, 4H), 7.41 (t, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;7.5 Hz, 1H), 7.27 (dd, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.5, 5.5 Hz, 2H), 6.95 (m, 2H), 4.05 (s, 1H), 3.96 (m, 1H), 3.87\u0026ndash;3.74 (m, 4H), 2.74 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;4.5 Hz, 2H). \u003csup\u003e13\u003c/sup\u003eC NMR (125 MHz, CHLOROFORM-\u003cem\u003eD\u003c/em\u003e) δ 176.10, 163.07, 161.12, 156.91, 155.49, 140.54, 135.05, 134.14, 131.35, 130.55, 130.09, 130.03, 128.90, 128.67, 125.97, 125.22, 123.71, 118.24, 115.39, 115.22, 76.47, 68.91, 53.12, 51.09. \u003csup\u003e19\u003c/sup\u003eFNMR (471 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ-115.56. HRMS (ESI)m/z. [M\u0026thinsp;+\u0026thinsp;H]\u003csup\u003e+\u003c/sup\u003e Calcd for C\u003csub\u003e25\u003c/sub\u003eH\u003csub\u003e23\u003c/sub\u003eFNO\u003csub\u003e4\u003c/sub\u003e. 420.16056, found 420.15924\u003c/p\u003e \u003cp\u003e2-(4-bromophenyl)-3-(3-(hexylamino)-2-hydroxypropoxy)-7-methoxy-4\u003cem\u003eH\u003c/em\u003e-chromen-4-one(\u003cb\u003eY15\u003c/b\u003e). White solid; m.p. 131.2-132.1℃; Yield 36%; \u003csup\u003e1\u003c/sup\u003eH NMR (500 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ 8.29\u0026ndash;8.23 (m, 1H), 8.00\u0026ndash;7.95 (m, 2H), 7.78\u0026ndash;7.58 (m, 4H), 7.50 (m, 2H), 7.14 (m, 2H), 6.68 (t, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;7.5 Hz, 1H), 6.62 (m, 2H), 4.17\u0026ndash;4.12 (m, 1H), 4.02 (m, 1H), 3.94 (m, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;10.8, 7.0 Hz, 1H), 3.31 (m, 1H), 3.23\u0026ndash;3.16 (m, 1H). \u003csup\u003e13\u003c/sup\u003eC NMR (125 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) δ 175.21, 164.70, 157.23, 155.15, 140.12, 132.16, 129.99, 129.27, 127.16, 125.86, 117.32, 115.30, 99.95, 75.26, 67.55, 56.07, 51.60, 49.67, 31.60, 28.31, 26.82, 22.64, 14.13. HRMS(ESI) m/z [M\u0026thinsp;+\u0026thinsp;H]\u003csup\u003e+\u003c/sup\u003e Calcd for C\u003csub\u003e25\u003c/sub\u003eH\u003csub\u003e31\u003c/sub\u003eBrNO\u003csub\u003e5\u003c/sub\u003e. 504.13801, found 504.14053\u003c/p\u003e \u003c/div\u003e \u003c/div\u003e\n\u003cp\u003e3-(3-(benzylamino)-2-hydroxypropoxy)-7-methoxy-2-(p-tolyl)-4\u003cem\u003eH\u003c/em\u003e-chromen-4-one(Y16).\u003c/p\u003e\n\u003cdiv class=\"Heading\"\u003eWhite solid; m.p. 112.8-113.7℃; Yield 57%; \u003csup\u003e1\u003c/sup\u003eH NMR (500 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) \u0026delta; 8.05 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;9.0 Hz, 1H), 7.92 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.5 Hz, 2H), 7.59 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.5 Hz, 2H), 7.31\u0026ndash;7.22 (m, 6H), 6.93 (dd, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;9.0, 2.5 Hz, 1H), 6.86 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;2.5 Hz, 1H), 4.06\u0026ndash;4.03 (m, 1H), 3.93 (dd, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;10.5, 3.0 Hz, 1H), 3.88 (s, 3H), 3.86\u0026ndash;3.75 (m, 4H), 2.78\u0026ndash;2.68 (m, 2H).\u003csup\u003e13\u003c/sup\u003eC NMR (125 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) \u0026delta; 175.20, 164.60, 157.18, 154.95, 140.43, 139.51, 132.09, 129.98, 128.54, 128.43, 127.26, 127.22, 125.70, 117.47, 115.17, 99.97, 76.40, 69.00, 56.07, 53.88, 51.14. HRMS(ESI) m/z [M\u0026thinsp;+\u0026thinsp;H]\u003csup\u003e+\u003c/sup\u003e Calcd for C\u003csub\u003e26\u003c/sub\u003eH\u003csub\u003e25\u003c/sub\u003eBrNO\u003csub\u003e5\u003c/sub\u003e. 510.09106, found 510.08975\u003c/div\u003e\n\u003cp\u003e3-(3-(hexylamino)-2-hydroxypropoxy)-2-(p-tolyl)-4\u003cem\u003eH\u003c/em\u003e-chromen-4-one(\u003cstrong\u003eY17\u003c/strong\u003e). Orange oily; Yield 61%; \u003csup\u003e1\u003c/sup\u003eH NMR (400 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) \u0026delta; 8.21 (dd, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.0, 1.6 Hz, 1H), 8.03 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.4 Hz, 2H), 7.79\u0026ndash;7.70 (m, 1H), 7.59 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.5 Hz, 1H), 7.45 (t, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;7.6 Hz, 1H), 7.35 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.0 Hz, 2H), 4.88 (s, 2H), 4.29 (t, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;4.8 Hz, 1H), 3.97 (m, 2H), 3.28 (m, 2H), 3.00 (m, 2H), 2.44 (s, 3H), 1.88 (m, 2H), 1.45\u0026ndash;1.28 (m, 6H), 0.92\u0026ndash;0.85 (m, 3H). \u003csup\u003e13\u003c/sup\u003eC NMR (100 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) \u0026delta; 176.09, 157.64, 155.43, 142.32, 139.51, 134.25, 129.72, 128.53, 127.12, 125.74, 125.26, 123.31, 118.21, 73.50, 66.53, 51.84, 49.41, 31.34, 26.95, 26.55, 22.52, 21.66, 14.02. HRMS(ESI) m/z [M\u0026thinsp;+\u0026thinsp;H]\u003csup\u003e+\u003c/sup\u003e Calcd for C\u003csub\u003e25\u003c/sub\u003eH\u003csub\u003e32\u003c/sub\u003eNO\u003csub\u003e4\u003c/sub\u003e. 410.23258, found 410.23117.\u003c/p\u003e\n\u003cp\u003e3-(3-(hexylamino)-2-hydroxypropoxy)-2-(2-methoxyphenyl)-4\u003cem\u003eH\u003c/em\u003e-chromen-4-one(\u003cstrong\u003eY18\u003c/strong\u003e). White solid; m.p. 136.7-137.5℃; Yield 64%; \u003csup\u003e1\u003c/sup\u003eH NMR (500 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) \u0026delta; 8.20 (dd, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.0, 1.5 Hz, 1H), 7.70 (m, 1H), 7.52\u0026ndash;7.40 (m, 4H), 7.10\u0026ndash;7.01 (m, 2H), 4.16 (m, 1H), 3.91\u0026ndash;3.82 (m, 5H), 3.27\u0026ndash;3.15 (m, 2H), 3.09\u0026ndash;2.97 (m, 2H), 1.90 (p, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.0 Hz, 2H), 1.41\u0026ndash;1.27 (m, 6H), 0.89\u0026ndash;0.82 (m, 3H). \u003csup\u003e13\u003c/sup\u003eC NMR (100 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) \u0026delta; 176.09, 157.64, 155.43, 142.32, 139.51, 134.25, 129.72, 128.53, 127.12, 125.74, 125.26, 123.31, 118.21, 73.50, 66.53, 51.84, 49.41, 31.34, 26.95, 26.55, 22.52, 21.66, 14.02. HRMS(ESI) m/z [M\u0026thinsp;+\u0026thinsp;H]\u003csup\u003e+\u003c/sup\u003e Calcd for C\u003csub\u003e25\u003c/sub\u003eH\u003csub\u003e32\u003c/sub\u003eNO\u003csub\u003e5\u003c/sub\u003e. 426.22750, found 426.22601.\u003c/p\u003e\n\u003cp\u003e2-(3-bromophenyl)-3-(3-(hexylamino)-2-hydroxypropoxy)-4\u003cem\u003eH\u003c/em\u003e-chromen-4-one(\u003cstrong\u003eY19\u003c/strong\u003e). Orange oily; Yield 31%; \u003csup\u003e1\u003c/sup\u003eH NMR (500 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) \u0026delta; 8.20\u0026ndash;8.05 (m, 3H), 7.75\u0026ndash;7.67 (m, 1H), 7.63\u0026ndash;7.51 (m, 2H), 7.44\u0026ndash;7.36 (m, 2H), 5.42 (s, 2H), 4.32 (t, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;5.0 Hz, 1H), 3.97 (t, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;5.5 Hz, 2H), 3.24 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;5.0 Hz, 2H), 2.99 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.5 Hz, 2H), 1.82\u0026ndash;1.79 (m, 1H), 1.36 (s, 1H), 1.26 (m, 6H), 0.86\u0026ndash;0.81 (m, 3H). \u003csup\u003e13\u003c/sup\u003eC NMR (125 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) \u0026delta; 175.89, 155.41, 155.28, 140.26, 134.55, 134.37, 132.04, 131.11, 130.68, 127.58, 125.87, 125.49, 123.45, 122.92, 118.30, 74.01, 66.41, 51.47, 49.34, 31.39, 26.87, 26.58, 22.57, 14.07. HRMS(ESI) m/z [M\u0026thinsp;+\u0026thinsp;H]\u003csup\u003e+\u003c/sup\u003e Calcd for C\u003csub\u003e24\u003c/sub\u003eH\u003csub\u003e29\u003c/sub\u003eBrNO\u003csub\u003e4\u003c/sub\u003e. 474.12745, found 474.12610.\u003c/p\u003e\n\u003cp\u003e3-3-(((4-fluorobenzyl)amino)-2-hydroxypropoxy)-2-(4-methoxyphenyl)-4\u003cem\u003eH\u003c/em\u003e-chromen-4-one (\u003cstrong\u003eY20\u003c/strong\u003e). White solid; m.p. 146.3-147.1℃; Yield 64%; \u003csup\u003e1\u003c/sup\u003eH NMR (500 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) \u0026delta; 8.05 (t, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.5 Hz, 3H), 7.68\u0026ndash;7.64 (m, 3H), 7.51 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.5 Hz, 1H), 7.38 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;7.5 Hz, 1H), 7.03\u0026ndash;6.97 (m, 4H), 4.37 (dd, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;25.0, 11.0 Hz, 4H), 3.94\u0026ndash;3.86 (m, 3H), 3.82 (s, 3H), 3.36 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;5.0 Hz, 1H), 2.37 (s, 1H). \u003csup\u003e13\u003c/sup\u003eC NMR (125 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) \u0026delta; 176.0, 175.8, 164.3, 162.3, 162.4, 157.6, 155.4, 155.3, 142.5, 139.4, 138.9, 134.38, 134.27, 132.84, 132.77, 130.56, 129.81, 128.58, 126.96, 126.72, 125.64, 125.59, 125.39, 125.34, 123.23, 123.18, 121.96, 118.28, 118.18, 116.18, 116.01, 114.56, 73.39, 73.26, 65.80, 55.60, 51.50, 50.56, 50.43, 21.69. \u003csup\u003e19\u003c/sup\u003eFNMR (471 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) \u0026delta; -111.52. HRMS (ESI) m/z [M\u0026thinsp;+\u0026thinsp;H]\u003csup\u003e+\u003c/sup\u003e Calcd for C\u003csub\u003e26\u003c/sub\u003eH\u003csub\u003e25\u003c/sub\u003eNO\u003csub\u003e5\u003c/sub\u003eF. 450.17113, found 450.16974.\u003c/p\u003e\n\u003cp\u003e3-(3-(hexylamino)-2-hydroxypropoxy)-2-(4-methoxyphenyl)-4\u003cem\u003eH\u003c/em\u003e-chromen-4-one(\u003cstrong\u003eY21\u003c/strong\u003e). Yellow oily; Yield 53%; \u003csup\u003e1\u003c/sup\u003eH NMR (500 MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) \u0026delta; 8.11 (m, 3H), 7.71\u0026ndash;7.67 (m, 1H), 7.53 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.5 Hz, 1H), 7.38 (m, 1H), 7.01 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;9.0 Hz, 2H), 4.38\u0026ndash;4.32 (m, 1H), 3.96 (t, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;5.0 Hz, 2H), 3.85 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;1.0 Hz, 3H), 3.38 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;5.0 Hz, 2H), 3.10 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;8.5 Hz, 2H), 1.90 (d, \u003cem\u003eJ\u003c/em\u003e\u0026thinsp;=\u0026thinsp;2.5 Hz, 1H), 1.49\u0026ndash;1.12 (m, 9H), 0.86\u0026ndash;0.83 (m, 3H). \u003csup\u003e13\u003c/sup\u003eC NMR (125MHz, CDCl\u003csub\u003e3\u003c/sub\u003e) \u0026delta; 175.95, 162.31, 157.49, 155.35, 138.99, 134.24, 130.58, 125.69, 125.28, 123.25, 122.07, 118.19, 114.56, 73.20, 65.96, 55.61, 51.72, 49.32, 31.30, 26.46, 26.30, 22.55, 14.07. HRMS(ESI) m/z [M\u0026thinsp;+\u0026thinsp;H]\u0026thinsp;+\u0026thinsp;Calcd for C\u003csub\u003e25\u003c/sub\u003eH\u003csub\u003e32\u003c/sub\u003eNO\u003csub\u003e5\u003c/sub\u003e. 426.22750, found 426.22626.\u003c/p\u003e\n\u003cdiv id=\"Sec25\" class=\"Section2\"\u003e\n \u003ch2\u003e4.3. \u003cstrong\u003ePlant Pathogenic Fungi\u003c/strong\u003e\u003c/h2\u003e\n \u003cdiv id=\"Sec26\" class=\"Section3\"\u003e\n \u003ch2\u003e4.3.1. Fungal sources\u003c/h2\u003e\n \u003cp\u003eThe fungi used in this study were all collected from natural habitats and kindly provided by the National Key Laboratory of Green Pesticide.\u003c/p\u003e\n \u003c/div\u003e\n \u003cdiv id=\"Sec27\" class=\"Section3\"\u003e\n \u003ch2\u003e4.3.2. Cultivation of fungi\u003c/h2\u003e\n \u003cp\u003eThe medium was boiled in the ratio of potato: glucose: agar\u0026thinsp;=\u0026thinsp;10: 10: 9, dispensed in 250 mL conical flasks and sterilized using autoclave at 121\u0026deg;C for 21 min, after completion of sterilization the cake which had been activated for 30 min was inoculated in the cooled medium and placed in the incubator for 5 d [\u003cspan class=\"CitationRef\"\u003e7\u003c/span\u003e, \u003cspan class=\"CitationRef\"\u003e31\u003c/span\u003e].\u003c/p\u003e\n \u003c/div\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec28\" class=\"Section2\"\u003e\n \u003ch2\u003e4.4 Antifungal test\u003c/h2\u003e\n \u003cdiv id=\"Sec29\" class=\"Section3\"\u003e\n \u003ch2\u003e4.4.1 Antifungal activity \u003cem\u003ein vitro\u003c/em\u003e\u003c/h2\u003e\n \u003cp\u003eThe fungicidal activity of the target compounds was determined by mycelial growth rate [\u003cspan class=\"CitationRef\"\u003e32\u003c/span\u003e]. The antifungal activity of \u003cstrong\u003eY1-Y21\u003c/strong\u003e at a concentration of 100 \u0026micro;g/mL was determined using mycelial growth method. The medium was prepared according to the ratio of potato dextrose agar (PDA), dispensed in 50 mL conical flasks and sterilized at 121 degrees celsius for 21 min. The compounds were dissolved in dimethyl sulfoxide (DMSO) and mixed with PDA to give a final concentration of 100 \u0026micro;g/mL, PDA containing the drug solution was poured into petri dishes and each set was repeated three times. The EC\u003csub\u003e50\u003c/sub\u003e of some of the compounds was further determined after screening the compounds with better activity.\u003c/p\u003e\n \u003cp\u003e\u003cimg src=\"data:image/png;base64,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\"\u003e\u003c/p\u003e\n \u003cp\u003e\u003cem\u003eI\u003c/em\u003e\u0026mdash;Inhibition rate\u003c/p\u003e\n \u003cp\u003e\u003cem\u003eC\u003c/em\u003e\u0026mdash;Blank control mycelium diameter\u003c/p\u003e\n \u003cp\u003e\u003cem\u003eT\u003c/em\u003e\u0026mdash; Mycelial diameter of drug treatment group\u003c/p\u003e\n \u003c/div\u003e\n \u003cdiv id=\"Sec30\" class=\"Section3\"\u003e\n \u003ch2\u003e4.4.2 Antifungal activity \u003cem\u003ein vivo\u003c/em\u003e\u003c/h2\u003e\n \u003cp\u003eThe curative and protective efficacy of \u003cstrong\u003eY17\u003c/strong\u003e against \u003cem\u003eP.c\u003c/em\u003e was assessed \u003cem\u003ein vivo\u003c/em\u003e, following the method reported in the literature. DMSO dissolved \u003cstrong\u003eY17\u003c/strong\u003e was formulated in different concentrations (100 and 200 \u0026micro;g/mL), fresh eggplant fruits were used as experimental material, the commercial drug, \u003cstrong\u003eAz\u003c/strong\u003e was used as a positive control group, and sterile distilled water (containing 0.2 mL of DMSO and 0.1% Tween \u0026minus;\u0026thinsp;80) was used as negative control. Curative activity was tested by punching a 5 mm hole in the surface of the eggplant, placing a cake of the fungus, and spraying the cake with \u003cstrong\u003eY17\u003c/strong\u003e solution after 24 h. Protective activity was tested by punching a 5 mm hole in the surface of the eggplant, spraying \u003cstrong\u003eY17\u003c/strong\u003e solution, and placing a cake of the fungus after 24 h. The area of infected eggplant fruits was measured after 5 d using the cross method [\u003cspan class=\"CitationRef\"\u003e33\u003c/span\u003e, \u003cspan class=\"CitationRef\"\u003e34\u003c/span\u003e].\u003c/p\u003e\n \u003cp\u003e\u003cimg 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\"\u003e\u003c/p\u003e\n \u003cp\u003e\u003cem\u003eC\u003c/em\u003e\u0026mdash;Control effect\u003c/p\u003e\n \u003cp\u003e\u003cem\u003eACK\u003c/em\u003e\u0026mdash;Blank control group lesion diameter\u003c/p\u003e\n \u003cp\u003e\u003cem\u003eA\u003c/em\u003e\u0026mdash;The diameter of the lesion after medication treatment.\u003c/p\u003e\n \u003c/div\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec31\" class=\"Section2\"\u003e\n \u003ch2\u003e4.5. Scanning electron microscope observation\u003c/h2\u003e\n \u003cp\u003e\u003cem\u003eP.c\u003c/em\u003e was inoculated on potato medium at 28℃ for 5 d, selected mycelia with a diameter of 5mm at the edge of the petri dish, picked 2\u0026ndash;3 cakes in 20 mL PDA medium, cultured at 28℃, 180 r for 3\u0026ndash;4 d, then added \u003cstrong\u003eY17\u003c/strong\u003e with DMSO solvent solution to make the final concentration of 0, 12.5, 25 \u0026micro;g/mL, under the same conditions, cultured for another 24 h. A small number of bacteria was picked and fixed with glutaraldehyde for 12 h, and the medium and other impurities were washed away with PBS. After replacing the medium with 30, 50, 70, 90, 100% for 2 times respectively, the bacteria were fixed with tert-butanol for 30 min (refrigerator freezing) and freeze-dried for 4 h. The bacteria were placed on conductive adhesive, sprayed with gold and the morphology of hyphae was observed in the scanning electron microscope [\u003cspan class=\"CitationRef\"\u003e35\u003c/span\u003e].\u003c/p\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec32\" class=\"Section2\"\u003e\n \u003ch2\u003e4.6. FM Observation.\u003c/h2\u003e\n \u003cp\u003e\u003cem\u003eP.c\u003c/em\u003e was inoculated on PDA medium at 28℃ for 5 d, selected mycelia with a diameter of 5mm at the edge of the petri dish, picked 2\u0026ndash;3 cakes in 20 mL PDA medium, incubated at 28℃, 180r for 3\u0026ndash;4 d, and then added the solution of \u003cstrong\u003eY17\u003c/strong\u003e solvented with DMSO to make the final concentration of 0, 50, 100 \u0026micro;g/mL, and incubated for another 24 h under the same conditions. A small amount of mycelium was picked and washed with PBS for 3 times, incubated with PI stain at 37\u0026deg;C for 15 min, then washed off the stain using PBS, and the mycelium was placed on slides for sampling with a sterile inoculation needle, and the mycelial morphology was observed under a fluorescence microscope [\u003cspan class=\"CitationRef\"\u003e36\u003c/span\u003e].\u003c/p\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec33\" class=\"Section2\"\u003e\n \u003ch2\u003e4.7. ROS accumulation\u003c/h2\u003e\n \u003cp\u003eReferring to the method in the literature [\u003cspan class=\"CitationRef\"\u003e37\u003c/span\u003e], the mycelium with the diameter of 5 mm at the edge of the petri dish was selected, and 2 cakes were picked in 25 mL PDA medium, incubated at 28℃, 180r for 2\u0026ndash;3 d, then \u003cstrong\u003eY17\u003c/strong\u003e solution was added to make the final concentration of 0, 50, 100 \u0026micro;g/mL, and incubated under the same conditions for another 24 h. A small amount of mycelium was picked and incubated with the staining agent for 30 min at 37℃, and the staining agent was washed away with PBS [\u003cspan class=\"CitationRef\"\u003e38\u003c/span\u003e]. The stain was washed off, and the mycelium was placed on a slide with a sterile inoculation needle to make a sample, and the mycelial morphology was observed under a fluorescence microscope.\u003c/p\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec34\" class=\"Section2\"\u003e\n \u003ch2\u003e4.8 Determination of leakage of intracellular solutes\u003c/h2\u003e\n \u003cp\u003eReferring to the experimental method in the literature [\u003cspan class=\"CitationRef\"\u003e39\u003c/span\u003e], a 5-mm cake of mycelium was selected and incubated with PDA medium at 28\u0026deg;C, 180 r for 4\u0026ndash;5 d, then washed with sterile water for three times and filtered; 100 mg of mycelium was weighed into 20 mL of sterile water, and DMSO-solubilized \u003cstrong\u003eY17\u003c/strong\u003e was added to obtain mycelial suspensions with solution concentrations of 12.5, 25, 50, and 100 \u0026micro;g/mL, respectively. The obtained suspensions were incubated at 28\u0026deg;C for 10 h. The absorbance of the supernatant was measured at 260 and 280 nm using a UV-Vis spectrophotometer.\u003c/p\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec35\" class=\"Section2\"\u003e\n \u003ch2\u003e4.9 Determination of cell membrane permeability\u003c/h2\u003e\n \u003cp\u003eReferring to the experimental method in the literature [\u003cspan class=\"CitationRef\"\u003e40\u003c/span\u003e], 5mm cakes of mycelium were selected and incubated with PDA medium at 28\u0026deg;C, 180 r for 4\u0026ndash;5 d, then 200 mg of mycelium was added to 20 mL of sterile water with DMSO-solubilized \u003cstrong\u003eY17\u003c/strong\u003e, to obtain mycelial suspensions with solution concentrations of 12.5, 25, 50, and 100 \u0026micro;g/mL, respectively, and the solutions were tested for their electrical conductivities at 1h intervals. After 6 h of testing, boiled in water at 100\u0026deg;C for 60 min, cooled, and measured its conductivity.\u003c/p\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec36\" class=\"Section2\"\u003e\n \u003ch2\u003e4.10 Cell dry weight experiment\u003c/h2\u003e\n \u003cp\u003eReferring to the literature method [\u003cspan class=\"CitationRef\"\u003e41\u003c/span\u003e], Capsicum blight fungus was inoculated on PDA medium for 5 d at 28\u0026deg;C. Mycelia with a diameter of 5 mm at the edge of the Petri dish were selected, and 2 cakes were picked in 20 mL of PDA medium, and then \u003cstrong\u003eY17\u003c/strong\u003e solution solubilized with DMSO was added to make the final concentration of 0, 3.125, 6.25, 12.5, and 25 \u0026micro;g/mL, after incubation at 28\u0026deg;C, 180 r for 3\u0026ndash;4 d, the sample was washed 3\u0026ndash;4 times with PBS, filtered, dried at 50\u0026deg;C and weighed.\u003c/p\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec37\" class=\"Section2\"\u003e\n \u003ch2\u003e4.11 Determination of MDA content\u003c/h2\u003e\n \u003cp\u003eMycobacterium chiliensis was cultured in sterile PDA medium and incubated in a constant temperature shaker at 25\u0026deg;C for 2\u0026ndash;3 d. Under the same conditions, the strains were incubated in \u003cstrong\u003eY17\u003c/strong\u003e solution containing different concentrations (0, 12.5, 25, 50, and 100 \u0026micro;g/mL) for 1 d, washed with sterile water, and the mycelia were collected and freeze-dried for 6 h. The dry weight of 100mg of mycelium under different treatments was weighed and experiments were carried out according to the kit instructions to determine the absorbance of the samples at 532 and 600 nm [\u003cspan class=\"CitationRef\"\u003e37\u003c/span\u003e].\u003c/p\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec38\" class=\"Section2\"\u003e\n \u003ch2\u003e4.12 Experimental safety tests\u003c/h2\u003e\n \u003cp\u003eReferring to previous literature reports [\u003cspan class=\"CitationRef\"\u003e42\u003c/span\u003e]. Pepper seeds were soaked with different concentrations of \u003cstrong\u003eY17\u003c/strong\u003e (0, 100, 200 and 400 \u0026micro;g/mL). The germination of chili pepper seeds was observed every 3 d, and the leaves and roots of chili pepper seedlings were sprayed with the above concentrations of \u003cstrong\u003eY17\u003c/strong\u003e for one consecutive week to observe the growth of the seedlings.\u003c/p\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec39\" class=\"Section2\"\u003e\n \u003ch2\u003e4.13 Energy calculation\u003c/h2\u003e\n \u003cp\u003eCalculate the molecular structure using Chemdraw. Then, single point energies were calculated by Gaussian view to obtain the desired energy parameters [\u003cspan class=\"CitationRef\"\u003e16\u003c/span\u003e]. Global reactivity descriptors HOMO and LUMO energies were used to define global reactivity descriptors such as softness (\u003cem\u003eS\u003c/em\u003e), global hardness (\u003cem\u003e\u0026eta;\u003c/em\u003e), chemical potential (\u0026micro;), electronegativity (\u003cem\u003e\u0026chi;\u003c/em\u003e), and global electrophilicity index (\u003cem\u003e\u0026omega;\u003c/em\u003e) using the following relational equation:\u003c/p\u003e\n \u003cp\u003e\u003cimg 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\"\u003e\u003c/p\u003e\n\u003c/div\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eAcknowledgement\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAuthors gratefully acknowledge the Science Foundation of Guizhou Province (No. ZK2024008),\u0026nbsp;Chinese Academy of Tropical Agricultural Sciences for Science and Technology Innovation Team of National Tropical Agricultural Science Center (No. CATASCXTD202410).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eSupporting information\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe Supporting Information includes the synthesis and characterization data, and NMR, HRMS spectrogram for the target compounds.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAuthors\u0026apos; contributions\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe current study is an outcome of constructive discussion with W.X;\u0026nbsp;Revised in full by X.H.R;\u0026nbsp;Y.J.Q and C.M.H performed synthesis and characterization experiments on the target compounds; Y.J.Q,Q.Z and Q.X.H completed experiments on preliminary screening and mechanism of action studies of antiviral activity; Y.J.Q, C.M.H and Q.Z carried out the NMR, and HRMS spectral analyses; Q.X.H prepared figures 1-8 and table 1-2; D,S were involved in the drafting of the manuscript and revising the manuscript. All authors read and approved the final manuscript.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConflicts of interest\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe authors declare that there are no competing financial interests.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\n\u003cli\u003eIsinkaye FO, Olusanya MO, Singh PK (2024) Deep learning and content-based filtering techniques for improving plant disease identification and treatment recommendations: A comprehensive review. Heliyon.10(9),29583-29602. https://doi.org/10.1016/j.heliyon.2024.e29583\u003c/li\u003e\n\u003cli\u003eWang QP, Zhang C, Wu XM, Long YH, Su Y (2021) Chitosan Augments Tetramycin against Soft Rot in Kiwifruit and Enhances Its Improvement for Kiwifruit Growth, Quality and Aroma. 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Redox Biology. 41,101944. https://doi.org/10.1016/j.redox.2021.101944\u003c/li\u003e\n\u003cli\u003eZhang MH, Feng S, Song JR, Ruan XH, Xue W (2024) Formononetin derivatives containing benzyl piperidine: A brand new, highly efficient inhibitor targeting Xanthomonas spp. Journal of Advanced Research.\u003cem\u003e \u003c/em\u003e2024(22)133-146. https://doi.org/10.1016/j.jare.2024.08.039 \u003c/li\u003e\n\u003cli\u003eZhou R, Zhan WL, Yuan CM, Zhang T, Mao P, Sun ZL, An YS, Xue W (2023) Design, Synthesis and Antifungal Activity of Novel 1,4-Pentadiene-3-one Containing Quinazolinone. International Journal of Molecular Sciences. 24 (3), 2599-2613.https://doi.org/ doi:10.3390/ijms24032599\u003c/li\u003e\n\u003cli\u003eMedin S, Dressel A, Specht DA, Sheppard TJ, Holycross ME, Reid MC, Gazel E, Wu M, Barstow B, Multiple Rounds of In Vivo Random Mutagenesis and Selection in Vibrio natriegens Result in Substantial Increases in REE Binding Capacity. 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Molecular Diversity. 2025-01-22.https://doi.org/10.1007/s11030-025-11109-6\u003c/li\u003e\n\u003c/ol\u003e"},{"header":"Tables","content":"\u003cp\u003e\u003cstrong\u003eTab. 1\u003c/strong\u003e Antifungal activities of \u003cstrong\u003eY1\u003c/strong\u003e-\u003cstrong\u003eY21\u003c/strong\u003e at 100 \u0026mu;g/mL \u003cem\u003ein vitro\u003c/em\u003e\u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e\n\u003ctable border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 55px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eCompd\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"8\" valign=\"top\" style=\"width: 477px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eInhibition rate (%)\u003csup\u003eb\u003c/sup\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 55px;\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u003cem\u003eP\u003c/em\u003e\u003c/strong\u003e\u003cstrong\u003e.\u003cem\u003ec\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u003cem\u003eS.s\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u003cem\u003eF.o\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u003cem\u003eP.s\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u003cem\u003eR.s\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u003cem\u003eB.d\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u003cem\u003eF.g\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u003cem\u003eF.m\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u003cem\u003eF.oc\u003c/em\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 55px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY1\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e28.1\u0026plusmn;3.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e38.8\u0026plusmn;2.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e31.5\u0026plusmn;3.4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e55.7\u0026plusmn;2.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e27.1\u0026plusmn;0.6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e14.9\u0026plusmn;1.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e34.7\u0026plusmn;2.5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e34.7\u0026plusmn;1.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e13.6\u0026plusmn;2.2\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 55px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY2\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e13.2\u0026plusmn;2.4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e8.8\u0026plusmn;1.2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e12.0\u0026plusmn;1.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e34.4\u0026plusmn;0.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e29.0\u0026plusmn;1.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e24.9\u0026plusmn;1.6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e12.4\u0026plusmn;1.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e21.9\u0026plusmn;2.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e12.8\u0026plusmn;2.6\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 55px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY3\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e14.5\u0026plusmn;3.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e14.2\u0026plusmn;3.5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e22.0\u0026plusmn;3.6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e41.8\u0026plusmn;3.5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e53.4\u0026plusmn;4.9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e38.2\u0026plusmn;4.2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e32.2\u0026plusmn;4.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e31.1\u0026plusmn;2.5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e9.9\u0026plusmn;4.6\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 55px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY4\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e17.8\u0026plusmn;2.6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e26.7\u0026plusmn;2.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e19.1\u0026plusmn;2.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e38.5\u0026plusmn;1.2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e56.1\u0026plusmn;2.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e24.5\u0026plusmn;2.9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e20.2\u0026plusmn;2.6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e18.6\u0026plusmn;3.2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n 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valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e26.4\u0026plusmn;3.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e9.9\u0026plusmn;2.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e15.9\u0026plusmn;4.3\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 55px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY6\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e40.5\u0026plusmn;3.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e40.0\u0026plusmn;4.2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e30.2\u0026plusmn;2.2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n 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valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e10.4\u0026plusmn;2.2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e11.8\u0026plusmn;3.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e20.5\u0026plusmn;2.6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e59.5\u0026plusmn;2.5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e14.5\u0026plusmn;4.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e21.9\u0026plusmn;4.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e55.4\u0026plusmn;3.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e5.4\u0026plusmn;2.9\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 55px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY8\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e62.8\u0026plusmn;2.2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e-\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e-\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e18.7\u0026plusmn;4.2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e51.9\u0026plusmn;1.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e-\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e34.3\u0026plusmn;1.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e14.9\u0026plusmn;3.6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e-\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 55px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY9\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e89.5\u0026plusmn;0.9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e12.5\u0026plusmn;3.4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e32.4\u0026plusmn;0.9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e-\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e17.9\u0026plusmn;0.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e11.7\u0026plusmn;2.2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e-\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e55.8\u0026plusmn;2.9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e19.8\u0026plusmn;2.3\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 55px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY10\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e69.0\u0026plusmn;1.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e36.7\u0026plusmn;2.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e13.7\u0026plusmn;1.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e27.8\u0026plusmn;3.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e41.2\u0026plusmn;1.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e2.8\u0026plusmn;1.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e14.5\u0026plusmn;2.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e46.3\u0026plusmn;1.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e12.4\u0026plusmn;3.6\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 55px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY11\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e96.7\u0026plusmn;1.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e93.3\u0026plusmn;1.9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e61.0\u0026plusmn;2.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e-\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e39.3\u0026plusmn;2.2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e34.1\u0026plusmn;1.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e40.9\u0026plusmn;2.9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e82.6\u0026plusmn;2.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e27.3\u0026plusmn;1.8\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 55px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY12\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e90.5\u0026plusmn;0.9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e96.3\u0026plusmn;3.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e43.2\u0026plusmn;1.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e93.8\u0026plusmn;0.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e64.1\u0026plusmn;3.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e66.7\u0026plusmn;1.6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e10.7\u0026plusmn;3.4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e40.5\u0026plusmn;1.9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e36.0\u0026plusmn;0.9\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 55px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY13\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e53.7\u0026plusmn;3.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e38.8\u0026plusmn;2.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e31.5\u0026plusmn;3.4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e25.6\u0026plusmn;1.5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e67.6\u0026plusmn;2.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e-\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e-\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e35.3\u0026plusmn;2.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e27.7\u0026plusmn;2.9\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 55px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY14\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e33.1\u0026plusmn;2.4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e61.5\u0026plusmn;1.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e-\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e36.6\u0026plusmn;2.2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e14.5\u0026plusmn;4.2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e5.6\u0026plusmn;1.6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e35.5\u0026plusmn;2.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e63.2\u0026plusmn;3.2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e-\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 55px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY15\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e93.5\u0026plusmn;2.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e54.1\u0026plusmn;3.4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e68.9\u0026plusmn;2.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e78.4\u0026plusmn;3.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e75.6\u0026plusmn;2.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e75.9\u0026plusmn;1.4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e59.5\u0026plusmn;2.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e57.4\u0026plusmn;1.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e67.8\u0026plusmn;2.0\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 55px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY16\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e92.6\u0026plusmn;2.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e96.3\u0026plusmn;1.2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e75.1\u0026plusmn;2.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e-\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e69.1\u0026plusmn;1.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e57.0\u0026plusmn;0.5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e33.6\u0026plusmn;0.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e40.2\u0026plusmn;1.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e35.5\u0026plusmn;2.4\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 55px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY17\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e100.0\u0026plusmn;0.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e95.4\u0026plusmn;3.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e41.5\u0026plusmn;5.4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e96.7\u0026plusmn;1.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e77.5\u0026plusmn;3.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e82.4\u0026plusmn;2.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e70.2\u0026plusmn;3.9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e69.0\u0026plusmn;3.6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e34.7\u0026plusmn;3.5\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 55px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY18\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e97.1\u0026plusmn;0.9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e83.8\u0026plusmn;1.2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e70.1\u0026plusmn;1.5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e91.6\u0026plusmn;0.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e60.7\u0026plusmn;2.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e52.9\u0026plusmn;0.9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e36.5\u0026plusmn;2.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e67.2\u0026plusmn;3.4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e30.2\u0026plusmn;1.2\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 55px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY19\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e91.3\u0026plusmn;0.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e97.5\u0026plusmn;0.4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e60.1\u0026plusmn;3.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e96.3\u0026plusmn;1.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e85.9\u0026plusmn;1.5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e62.2\u0026plusmn;2.2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e31.4\u0026plusmn;1.5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e77.3\u0026plusmn;2.9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e30.6\u0026plusmn;2.0\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 55px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY20\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e71.5\u0026plusmn;1.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e98.2\u0026plusmn;0.6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e63.9\u0026plusmn;9.4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e93.4\u0026plusmn;0.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e11.5\u0026plusmn;0.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e66.0\u0026plusmn;0.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e74.4\u0026plusmn;1.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e79.0\u0026plusmn;1.2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e45.5\u0026plusmn;1.4\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 55px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY21\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e92.6\u0026plusmn;2.4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e87.1\u0026plusmn;2.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e55.0\u0026plusmn;2.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e87.5\u0026plusmn;1.2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e60.3\u0026plusmn;2.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e74.3\u0026plusmn;1.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e67.4\u0026plusmn;0.9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e67.5\u0026plusmn;0.4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e38.0\u0026plusmn;2.0\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 55px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eAz\u003csup\u003ec\u003c/sup\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e48.9\u0026plusmn;2.4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e88.8\u0026plusmn;2.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e78.3\u0026plusmn;2.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e60.6\u0026plusmn;3.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e76.3\u0026plusmn;3.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e69.1\u0026plusmn;2.7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e72.7\u0026plusmn;2.4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e64.7\u0026plusmn;1.6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 60px;\"\u003e\n \u003cp\u003e60.6\u0026plusmn;3.7\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003csup\u003ea\u0026nbsp;\u003c/sup\u003eVales are mean \u0026plusmn; SD of three replicates.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cem\u003e\u003csup\u003eb\u003c/sup\u003e\u003c/em\u003e\u003cem\u003eR.s\u0026nbsp;\u003c/em\u003e(\u003cem\u003eR.solani\u003c/em\u003e),\u003cem\u003e\u0026nbsp;S.s\u0026nbsp;\u003c/em\u003e(\u003cem\u003eSsclerotiorm\u003c/em\u003e)\u003cem\u003e, F.g\u0026nbsp;\u003c/em\u003e(\u003cem\u003eF.graminearm\u003c/em\u003e)\u003cem\u003e, P.c\u0026nbsp;\u003c/em\u003e(\u003cem\u003eP.capsici\u003c/em\u003e)\u003cem\u003e, F.oc\u003c/em\u003e (\u003cem\u003eF.oxysporum cucumerinum\u003c/em\u003e)\u003cem\u003e, P.s\u003c/em\u003e (\u003cem\u003ePhomopsis.sp), F.o\u0026nbsp;\u003c/em\u003e(\u003cem\u003eF.oxysporum\u003c/em\u003e)\u003cem\u003e, F.m\u0026nbsp;\u003c/em\u003e( \u003cem\u003eT. Botrytis cinerea\u003c/em\u003e), \u003cem\u003eB.d (B. dothidea)\u003c/em\u003e\u003c/p\u003e\n\u003cp\u003e\u003csup\u003ec\u0026nbsp;\u003c/sup\u003eThe commercial antifungal agent Azoxystrobin.\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003cstrong\u003eTab. 2\u0026nbsp;\u003c/strong\u003eThe EC\u003csub\u003e50\u003c/sub\u003e values of some target compounds\u003csup\u003ea\u003c/sup\u003e.\u003c/p\u003e\n\u003ctable border=\"1\" cellspacing=\"0\" cellpadding=\"0\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 73px;\"\u003e\n \u003cp\u003e\u0026nbsp; Compd\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 121px;\"\u003e\n \u003cp\u003ePhytopathogen\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 171px;\"\u003e\n \u003cp\u003eRegression equation\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 74px;\"\u003e\n \u003cp\u003eR\u003csup\u003e2\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 115px;\"\u003e\n \u003cp\u003eEC\u003csub\u003e50\u003c/sub\u003e(\u0026mu;g/mL)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 73px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY14\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 121px;\"\u003e\n \u003cp\u003e\u003cem\u003eP.c\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 171px;\"\u003e\n \u003cp\u003ey = 2.7324x + 2.3199\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 74px;\"\u003e\n \u003cp\u003e0.9939\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 115px;\"\u003e\n \u003cp\u003e9.5\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 73px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY17\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 121px;\"\u003e\n \u003cp\u003e\u003cem\u003eP.c\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 171px;\"\u003e\n \u003cp\u003ey = 1.9978x + 3.5650\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 74px;\"\u003e\n \u003cp\u003e0.9911\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 115px;\"\u003e\n \u003cp\u003e5.2\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 73px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY18\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 121px;\"\u003e\n \u003cp\u003e\u003cem\u003eP.c\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 171px;\"\u003e\n \u003cp\u003ey = 2.1142x + 2.9669\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 74px;\"\u003e\n \u003cp\u003e0.9554\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 115px;\"\u003e\n \u003cp\u003e9.1\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 73px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY21\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 121px;\"\u003e\n \u003cp\u003e\u003cem\u003eP.c\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 171px;\"\u003e\n \u003cp\u003ey = 3.1658x + 2.2059\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 74px;\"\u003e\n \u003cp\u003e0.9759\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 115px;\"\u003e\n \u003cp\u003e7.6\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 73px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eAZ\u003c/strong\u003e\u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 121px;\"\u003e\n \u003cp\u003e\u003cem\u003eP.c\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 171px;\"\u003e\n \u003cp\u003ey = 1.9578x + 1.0977\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 74px;\"\u003e\n \u003cp\u003e0.9833\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 115px;\"\u003e\n \u003cp\u003e98.5\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 73px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY12\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 121px;\"\u003e\n \u003cp\u003e\u003cem\u003eS.s\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 171px;\"\u003e\n \u003cp\u003ey = 2.8155x + 1.8263\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 74px;\"\u003e\n \u003cp\u003e0.9465\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 115px;\"\u003e\n \u003cp\u003e13.4\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 73px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY16\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 121px;\"\u003e\n \u003cp\u003e\u003cem\u003eS.s\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 171px;\"\u003e\n \u003cp\u003ey = 2.7066x + 1.4513\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 74px;\"\u003e\n \u003cp\u003e0.9925\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 115px;\"\u003e\n \u003cp\u003e20.47\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 73px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY17\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 121px;\"\u003e\n \u003cp\u003e\u003cem\u003eS.s\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 171px;\"\u003e\n \u003cp\u003ey = 3.0669x + 1.2437\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 74px;\"\u003e\n \u003cp\u003e0.9684\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 115px;\"\u003e\n \u003cp\u003e16.8\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 73px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY18\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 121px;\"\u003e\n \u003cp\u003e\u003cem\u003eS.s\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 171px;\"\u003e\n \u003cp\u003ey = 3.4447x + 0.3402\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 74px;\"\u003e\n \u003cp\u003e0.9248\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 115px;\"\u003e\n \u003cp\u003e22.5\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 73px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY20\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 121px;\"\u003e\n \u003cp\u003e\u003cem\u003eS.s\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 171px;\"\u003e\n \u003cp\u003ey = 2.6969x + 2.2423\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 74px;\"\u003e\n \u003cp\u003e0.9799\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 115px;\"\u003e\n \u003cp\u003e10.5\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 73px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eAZ\u003c/strong\u003e\u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 121px;\"\u003e\n \u003cp\u003e\u003cem\u003eS.s\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 171px;\"\u003e\n \u003cp\u003ey = 1.2227x + 2.9211\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 74px;\"\u003e\n \u003cp\u003e0.9348\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 115px;\"\u003e\n \u003cp\u003e50.1\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 73px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY12\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 121px;\"\u003e\n \u003cp\u003e\u003cem\u003eP.s\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 171px;\"\u003e\n \u003cp\u003ey = 2.7915x + 1.3955\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 74px;\"\u003e\n \u003cp\u003e0.9815\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 115px;\"\u003e\n \u003cp\u003e19.5\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 73px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY14\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 121px;\"\u003e\n \u003cp\u003e\u003cem\u003eP.s\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 171px;\"\u003e\n \u003cp\u003ey = 2.9388x + 0.7153\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 74px;\"\u003e\n \u003cp\u003e0.9783\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 115px;\"\u003e\n \u003cp\u003e28.7\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 73px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY17\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 121px;\"\u003e\n \u003cp\u003e\u003cem\u003eP.s\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 171px;\"\u003e\n \u003cp\u003ey = 2.3055x + 2.0313\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 74px;\"\u003e\n \u003cp\u003e0.9734\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 115px;\"\u003e\n \u003cp\u003e19.3\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 73px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY18\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 121px;\"\u003e\n \u003cp\u003e\u003cem\u003eP.s\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 171px;\"\u003e\n \u003cp\u003ey = 2.7725x + 0.9446\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 74px;\"\u003e\n \u003cp\u003e0.9602\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 115px;\"\u003e\n \u003cp\u003e29.0\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 73px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY20\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 121px;\"\u003e\n \u003cp\u003e\u003cem\u003eP.s\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 171px;\"\u003e\n \u003cp\u003ey = 2.6263x + 1.6147\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 74px;\"\u003e\n \u003cp\u003e0.9718\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 115px;\"\u003e\n \u003cp\u003e19.4\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 73px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eAZ\u003csup\u003eb\u003c/sup\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 121px;\"\u003e\n \u003cp\u003e\u003cem\u003eP.s\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 171px;\"\u003e\n \u003cp\u003ey = 1.4157x + 2.5526\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 74px;\"\u003e\n \u003cp\u003e0.9448\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 115px;\"\u003e\n \u003cp\u003e53.5\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003csup\u003ea\u0026nbsp;\u003c/sup\u003eVales are mean \u0026plusmn; SD of three replicates.\u003csup\u003e\u0026nbsp;b\u003c/sup\u003eThe commercial antifungal agent Azoxystrobin.\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003cstrong\u003eTab. 3\u003c/strong\u003e The Protective and curative activities of \u003cstrong\u003eY17\u003c/strong\u003e and \u003cstrong\u003eAz\u003c/strong\u003e against \u003cem\u003eP.c\u003c/em\u003e \u003cem\u003ein vivo\u003csup\u003ea\u003c/sup\u003e\u003c/em\u003e\u003c/p\u003e\n\u003cdiv align=\"center\"\u003e\n \u003ctable border=\"1\" cellspacing=\"0\" cellpadding=\"0\" width=\"102%\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 10px;\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 16px;\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eCurativeactivity\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd colspan=\"2\" valign=\"top\" style=\"width: 40px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eProtectiv\u003c/strong\u003e\u003cstrong\u003ee\u0026nbsp;\u003c/strong\u003e\u003cstrong\u003eactivity\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 10px;\"\u003e\n \u003cp\u003eCompd\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 16px;\"\u003e\n \u003cp\u003eConcentration\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 16px;\"\u003e\n \u003cp\u003eLesionlength\u003csup\u003ea\u0026nbsp;\u003c/sup\u003e(mm\u0026plusmn;SD)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 16px;\"\u003e\n \u003cp\u003eControlling efffcacy (%)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 18px;\"\u003e\n \u003cp\u003eLesionlength\u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e\n \u003cp\u003e(mm\u0026plusmn;SD)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 21px;\"\u003e\n \u003cp\u003eControlling\u003c/p\u003e\n \u003cp\u003eefffcacy(%)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 10px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY17\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 16px;\"\u003e\n \u003cp\u003e200.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 16px;\"\u003e\n \u003cp\u003e15.4\u0026plusmn;0.9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 16px;\"\u003e\n \u003cp\u003e77.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 18px;\"\u003e\n \u003cp\u003e9.0\u0026plusmn;1.3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 21px;\"\u003e\n \u003cp\u003e85.7\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 10px;\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 16px;\"\u003e\n \u003cp\u003e100.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 16px;\"\u003e\n \u003cp\u003e3.0\u0026plusmn;1.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 16px;\"\u003e\n \u003cp\u003e38.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 18px;\"\u003e\n \u003cp\u003e24.3\u0026plusmn;1.5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 21px;\"\u003e\n \u003cp\u003e45.7\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 10px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eAz\u003c/strong\u003e\u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 16px;\"\u003e\n \u003cp\u003e200.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 16px;\"\u003e\n \u003cp\u003e39.4\u0026plusmn;1.5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 16px;\"\u003e\n \u003cp\u003e35.5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 18px;\"\u003e\n \u003cp\u003e25.7\u0026plusmn;0.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 21px;\"\u003e\n \u003cp\u003e42.9\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 10px;\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 16px;\"\u003e\n \u003cp\u003e100.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 16px;\"\u003e\n \u003cp\u003e42.4\u0026plusmn;0.6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 16px;\"\u003e\n \u003cp\u003e22.2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 18px;\"\u003e\n \u003cp\u003e32.1\u0026plusmn;0.9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 21px;\"\u003e\n \u003cp\u003e22.9\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 10px;\"\u003e\n \u003cp\u003eControl\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 16px;\"\u003e\n \u003cp\u003e0.0\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 16px;\"\u003e\n \u003cp\u003e50.0\u0026plusmn;0.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 16px;\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 18px;\"\u003e\n \u003cp\u003e39.4\u0026plusmn;1.1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 21px;\"\u003e\n \u003cp\u003e\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n \u003c/table\u003e\n\u003c/div\u003e\n\u003cp\u003e\u003csup\u003ea\u0026nbsp;\u003c/sup\u003eValues are mean \u0026plusmn; SD of three replicates. \u003csup\u003eb\u003c/sup\u003eThe commercial antifungal agent Azoxystrobin.\u003c/p\u003e\n\u003cp\u003e\u0026nbsp;\u003cstrong\u003eTab. 4\u003c/strong\u003e Physicochemical property comparison of \u003cstrong\u003eY17\u003c/strong\u003e and\u003cstrong\u003e\u0026nbsp;Az\u003c/strong\u003e\u003c/p\u003e\n\u003ctable border=\"1\" cellspacing=\"0\" cellpadding=\"0\" width=\"100%\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eParameter\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eAz\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e\u003cstrong\u003eY17\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003eMV\u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e403.39\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e409.53\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003eArs\u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e2\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e\u003cem\u003eE\u003c/em\u003e\u003csub\u003eTotal\u003c/sub\u003e\u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e-1389.0443\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e-1327.1366\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e\u003cem\u003eE\u003c/em\u003e\u003csub\u003eHOMO\u003c/sub\u003e\u003csup\u003eb\u003c/sup\u003e(Eh)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e-0.2266\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e-0.2224\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e\u003cem\u003eE\u003c/em\u003e\u003csub\u003eLUMO\u003c/sub\u003e\u003csup\u003eb\u003c/sup\u003e(Eh)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e-0.0538\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e-0.0776\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e\u003cem\u003eE\u003c/em\u003ea\u003csup\u003eb\u003c/sup\u003e(Eh)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e0.1728\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e0.1448\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e\u003cem\u003e\u0026chi;\u003c/em\u003e\u003csup\u003ec\u003c/sup\u003e(eV)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e3.8150\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e4.0817\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e\u003cem\u003e\u0026mu;\u003c/em\u003e\u003csup\u003ec\u003c/sup\u003e(eV)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e-3.8150\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e-4.0817\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e\u003cem\u003e\u0026eta;\u003c/em\u003e\u003csup\u003ec\u003c/sup\u003e(eV)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e2.3511\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e1.9701\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e\u003cem\u003eS\u003c/em\u003e\u003csup\u003ec\u003c/sup\u003e(eV)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e0.2127\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e0.2538\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e\u003cem\u003e\u0026omega;\u003c/em\u003e\u003csup\u003ec\u003c/sup\u003e(eV)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e3.0953\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd valign=\"top\" style=\"width: 33px;\"\u003e\n \u003cp\u003e4.2283\u0026nbsp;\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003csup\u003ea\u003c/sup\u003e Calculate the molecular structure using Chemdraw\u003c/p\u003e\n\u003cp\u003e\u003csup\u003eb\u003c/sup\u003e Gaussian view were used to obtain the molecular energy and energy orbital values.\u003c/p\u003e\n\u003cp\u003e\u003csup\u003ec\u003c/sup\u003e The unit is converted from Hartree (Eh) to eV, 1 Eh\u0026thinsp;=\u0026thinsp;27.211\u0026thinsp;eV\u003c/p\u003e"},{"header":"Scheme 1","content":"\u003cp\u003eScheme 1 is available in the Supplementary Files section.\u003c/p\u003e\n"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"flavonols, β-amino alcohols, phytophthora capsica (P.c), mechanism of fungal activity","lastPublishedDoi":"10.21203/rs.3.rs-6989432/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-6989432/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eA series of flavonoid derivatives containing \u003cem\u003eβ\u003c/em\u003e-amino alcohols were designed and synthesized using the natural product flavonols as the lead compounds, and their structures were verified by NMR and HRMS, and the antimicrobial activities of 21 flavonoid derivatives containing \u003cem\u003eβ\u003c/em\u003e-amino alcohols were systematically evaluated against nine plant pathogenic bacteria. Among the tested compounds, some of them showed good inhibitory effects against \u003cem\u003ePhytophthora capsici\u003c/em\u003e (\u003cem\u003eP.c\u003c/em\u003e), \u003cem\u003eSclerotinia mycoides\u003c/em\u003e (\u003cem\u003eS.s\u003c/em\u003e), and \u003cem\u003ePhytophthora kiwifruit anopheles\u003c/em\u003e (\u003cem\u003eP.s\u003c/em\u003e). It is noteworthy that compound \u003cb\u003eY17\u003c/b\u003e exhibited significant inhibitory activity against \u003cem\u003eP.c\u003c/em\u003e. At a treatment concentration of 100 \u0026micro;g/mL, \u003cb\u003eY17\u003c/b\u003e inhibited mycelial growth by 100%, and its half effective concentration (EC\u003csub\u003e50\u003c/sub\u003e) was 5.2 \u0026micro;g/mL, which was significantly better than that of the \u003cb\u003eazoxystrobin\u003c/b\u003e (\u003cb\u003eAz\u003c/b\u003e, 98.4 \u0026micro;g/mL). In addition, at a concentration of 200 \u0026micro;g/mL, the protective (85.7%) and curative (77.8%) effects of \u003cb\u003eY17\u003c/b\u003e against eggplant fruit blight were significantly higher than those of the \u003cb\u003eAz\u003c/b\u003e (42.9% protective and 35.5% curative effects). Scanning electron microscopy (SEM) further confirmed the disruption of bacterial membrane integrity by \u003cb\u003eY17\u003c/b\u003e. The kinetic simulations showed that \u003cb\u003eY17\u003c/b\u003e has similarity in nature and energy with the commercial drug \u003cb\u003eAz\u003c/b\u003e. Therefore, flavonols derivatives containing \u003cem\u003eβ\u003c/em\u003e-amino alcohols have a high probability to be used as potential antifungal agents.\u003c/p\u003e","manuscriptTitle":"Design, synthesis and bioactivity studies of flavonol bio-containing β-amino alcohols","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-07-03 11:40:57","doi":"10.21203/rs.3.rs-6989432/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"8a1f6c14-865a-4826-85bd-4a9928d3abdc","owner":[],"postedDate":"July 3rd, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2025-07-22T20:38:28+00:00","versionOfRecord":[],"versionCreatedAt":"2025-07-03 11:40:57","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-6989432","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-6989432","identity":"rs-6989432","version":["v1"]},"buildId":"XKTyCvWXoU3ODBz1xrDgd","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}