Rosaniline Hydrochloride Encapsulated MCM-48: Fluorescent and Electrochemical Sensor for Dopamine

Abstract The dye Rosaniline hydrochloride (RANH) has been successfully incorporated in MCM-48 (designated as RANH@MCM-48) and characterised by various spectroscopic methods including FT-IR, SEM, EDX and N2 adsorption-desorption isotherm. RANH@MCM-48 in aqueous medium acts as fluorescence “on” sensor for neurotransmitter dopamine (DA) in presence of its main biological interfering agents ascorbic acid or vitamin c (AA) along with Glucose, Cholesterol and Uric acid. The limits of detection (LOD) were found to be 65 nM and 51 nM respectively in absence and in presence of AA. The binding of DA to RANH@MCM-48 is found to be reversible with respect to EDTA2-. The fluorescence intensity vs. pH plot shows a narrow fluorescence window of 7.2 to 8.8. RANH@MCM-48 has been successfully applied for DA detection in artificial cerebrospinal fluid (ACF) and bovine serum albumin (BSA) with LOD values 27 nM and 22.5 nM respectively. Platinum disc electrode has been modified with RANH@MCM-48 which showed distinct oxidation peaks with a separation of 0.188 V in cyclic voltammetry (CV). The LOD for DA in presence of AA determined from oxidation current is 77.5 nM. The voltammetric detection of DA is found to be free from common interfering species Na+, K+, Ca2+, Fe2+, Uric acid, Cholesterol and Glucose.