Trace Analysis of Emerging Virus: an Ultrasensitive ECL-Scan Imaging System for Viral Infectious Disease

Trace Analysis of Emerging Virus: an Ultrasensitive ECL-Scan Imaging System for Viral Infectious Disease | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Trace Analysis of Emerging Virus: an Ultrasensitive ECL-Scan Imaging System for Viral Infectious Disease Yunxia Wu, Qikang Wu, Yu Fu, Mingxing Huang, Zhijin Fan, Bowen Shu, and 6 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-373673/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background Emerging infectious disease have brought a huge impact on human society in recent years. The outbreak of Zika virus (ZIKV) in the Americas resulted in a large number of babies born with microcephaly. More seriously, the Coronavirus Disease 2019 (COVID-19) caused the global spreads and immeasurable damages. Thus, the monitoring of highly pathogenic virus is of significance to the prevention and control of emerging infectious disease. Results Herein, a dendritic polymer probe-amplified ECL-scan imaging system was constructed to realize trace analysis of viral emerging infectious disease. Dendritic polymer probe was employed as the efficient signal giving-out component that could generate amplified electrochemiluminescence (ECL) signal on the integrated chip. And the signal was detected by a single-photon level charge coupled device-based ECL-scan imaging system. With this strategy, the ZIKV in the complex system of blood, urine and saliva were detected. The results indicated that high sensitivity of 50 copies and superior specificity were achieved. Furthermore, this strategy realized highly sensitive detection (10 copies) of S and N protein gene sequence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-Cov2) and spiked pseudovirus samples. Conclusions Thus, the dendritic polymer probe-amplified ECL-scan imaging system suitably met the strict clinical-requirements for trace analysis of emerging virus, and thus has the potential to serve as a paradigm for monitoring of emerging infectious disease. Nanoscience Emerging Virus Amplified Electrochemiluminescence Viral Infectious Disease ECL-Scan Imaging System Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Full Text Supplementary Files Supportinginformation.pdf Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-373673","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research","associatedPublications":[],"authors":[{"id":18962903,"identity":"8eb75cf6-1dd6-4f27-b5d3-8eb054a9ac17","order_by":0,"name":"Yunxia Wu","email":"","orcid":"","institution":"the First People's Hospital of Foshan","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Yunxia","middleName":"","lastName":"Wu","suffix":""},{"id":18962904,"identity":"65419426-87aa-4d5d-a39e-680c69f4a1a4","order_by":1,"name":"Qikang Wu","email":"","orcid":"","institution":"the First People's Hospital of 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A. Formation process of dendritic\npolymer probe. B. ECL detection process of dendritic Ru(bpy)32+-polymer amplified ECL-scan imaging system. C. Structure of ECLscan\nimaging system. D. Structure of ECL-scan imaging system. E. Details of ECL chip.","description":"","filename":"1.jpg","url":"https://assets-eu.researchsquare.com/files/rs-373673/v1/ddad6718f7d03deabd06dfe0.jpg"},{"id":7591446,"identity":"5cbc5c48-95db-451b-966f-62f13e6c5480","added_by":"auto","created_at":"2021-04-01 21:18:51","extension":"jpg","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":114263,"visible":true,"origin":"","legend":"Characterization of Dendritic Ru(bpy)3\n2+-Polymer. A. Molecular structure and activation process of Ru(bpy)32+. B.\nFluorescence characterization of Ru(bpy)32+ and the activated Ru(bpy)32+. C. Absorption characterization of Ru(bpy)32+ and the\nactivated Ru(bpy)32+. D. Absorption characterization of dendritic Ru(bpy)32+-polymer probe. E. Excitation and emission of dendritic\nRu(bpy)32+-polymer probe. F. Morphology of dendritic Ru(bpy)32+-polymer probe under atomic force microscope (AFM).","description":"","filename":"2.jpg","url":"https://assets-eu.researchsquare.com/files/rs-373673/v1/13f131df3f1b6f5601e2a31e.jpg"},{"id":7591027,"identity":"3b09937b-1242-4b47-962a-4c1373f94d7b","added_by":"auto","created_at":"2021-04-01 21:15:51","extension":"jpg","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":83973,"visible":true,"origin":"","legend":"Principle Validation of Dendritic Ru(bpy)3\n2+-Polymer Amplified ECL-scan Imaging System. A. ECL generation\ncomplex with streptavidin-labeled magnetic beads under light field. B. ECL signal detection of ECL generation complex under dark\nfield. C. Particle size and Zeta potential of streptavidin-labeled magnetic beads and ECL generation complex with streptavidin-labeled\nmagnetic beads. D. Optimization of the time of data acquisition.","description":"","filename":"3.jpg","url":"https://assets-eu.researchsquare.com/files/rs-373673/v1/b1671e22e77e9e5f21667c01.jpg"},{"id":7591018,"identity":"4c57b44e-0ebc-4ade-a452-e74dab9fce1f","added_by":"auto","created_at":"2021-04-01 21:15:51","extension":"jpg","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":78701,"visible":true,"origin":"","legend":"Optimization, Sensitivity and Specificity of Dendritic Ru(bpy)3\n2+-Polymer Amplified ECL-scan Imaging System. A.\nOptimization of hybridization time. B. Optimization of electrode voltage. C. Optimization of washing time. D. Sensitivity and linear\nanalysis of dendritic Ru(bpy)32+-polymer amplified ECL-scan imaging system. E. Specificity of dendritic Ru(bpy)32+-polymer\namplified ECL-scan imaging system with S1 and S2. F. Specificity of dendritic Ru(bpy)32+-polymer amplified ECL-scan imaging\nsystem with random sequence of RS1 and RS2.","description":"","filename":"4.jpg","url":"https://assets-eu.researchsquare.com/files/rs-373673/v1/bb3523f71221e7837ffd4e7e.jpg"},{"id":7591045,"identity":"3b0af912-cc53-4ccf-94a7-c48909b0d700","added_by":"auto","created_at":"2021-04-01 21:15:54","extension":"jpg","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":202857,"visible":true,"origin":"","legend":"Sequence of ZIKV and Other Flavivirus Strains, Sequence of Capture Probe and Signal Probe, Kinetic Parameters\nand Hybrid Structure.","description":"","filename":"5.jpg","url":"https://assets-eu.researchsquare.com/files/rs-373673/v1/8fb8201e98632204fe9d8c27.jpg"},{"id":7591036,"identity":"9bc97f5f-e78d-4466-9c86-d067d78dfa8c","added_by":"auto","created_at":"2021-04-01 21:15:52","extension":"jpg","order_by":6,"title":"Figure 6","display":"","copyAsset":false,"role":"figure","size":167925,"visible":true,"origin":"","legend":"Performance Evaluation of Dendritic Ru(bpy)3\n2+-Polymer Amplified ECL-scan Imaging System for ZIKV. A.\nSensitivity and specificity of dendritic Ru(bpy)32+-polymer amplified ECL-scan imaging system for ZIKV. B. Detection of blood\nsamples of mice. C. Detection of urine samples of mice. D to F. Detection of ZIKV spiked human blood samples. G to I. Detection\nof ZIKV spiked human urine samples.","description":"","filename":"6.jpg","url":"https://assets-eu.researchsquare.com/files/rs-373673/v1/1f21aac89d6f818794472487.jpg"},{"id":7591447,"identity":"4445565c-bd96-4c3b-a35a-e3f1453c8c1d","added_by":"auto","created_at":"2021-04-01 21:18:52","extension":"jpg","order_by":7,"title":"Figure 7","display":"","copyAsset":false,"role":"figure","size":126483,"visible":true,"origin":"","legend":"Performance Evaluation of Dendritic Ru(bpy)3\n2+-Polymer Amplified ECL-scan Imaging System for SARS-Cov2. A.\nSelected sequence of S protein and N protein gene, the capture and signal probe, kinetic parameters and hybrid structure. B.\nSensitivity of dendritic Ru(bpy)3\n2+-polymer amplified ECL-scan imaging system for SARS-Cov2. C. Specificity of dendritic\nRu(bpy)3\n2+-polymer amplified ECL-scan imaging system for SARS-Cov2. D. Detection of spiked saliva samples. E. Detection\nof spiked blood samples.","description":"","filename":"7.jpg","url":"https://assets-eu.researchsquare.com/files/rs-373673/v1/6557a117d57320f0cb8f0209.jpg"},{"id":13615596,"identity":"4be40791-7434-4f57-8513-c0396567e8e2","added_by":"auto","created_at":"2021-09-17 06:46:10","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":2572340,"visible":true,"origin":"","legend":"","description":"","filename":"ManuscriptFile.pdf","url":"https://assets-eu.researchsquare.com/files/rs-373673/v1_covered.pdf"},{"id":7590920,"identity":"b5728be3-56d3-414e-b3ef-68056f59528d","added_by":"auto","created_at":"2021-04-01 21:15:27","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":2699558,"visible":true,"origin":"","legend":"","description":"","filename":"ManuscriptFile.pdf","url":"https://assets-eu.researchsquare.com/files/rs-373673/v1_stamped.pdf"},{"id":7591474,"identity":"61bc1462-34fa-45fa-8239-2187452311e7","added_by":"auto","created_at":"2021-04-01 21:18:57","extension":"pdf","order_by":5,"title":"","display":"","copyAsset":false,"role":"supplement","size":477320,"visible":true,"origin":"","legend":"","description":"","filename":"Supportinginformation.pdf","url":"https://assets-eu.researchsquare.com/files/rs-373673/v1/12579eb9fc452008889198a3.pdf"}],"financialInterests":"","formattedTitle":"Trace Analysis of Emerging Virus: an Ultrasensitive ECL-Scan Imaging System for Viral Infectious Disease","fulltext":[{"header":"Full Text","content":"This preprint is available for \u003ca href='/article/rs-373673/latest.pdf' target='_blank'\u003edownload as a PDF\u003c/a\u003e."}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Emerging Virus, Amplified Electrochemiluminescence, Viral Infectious Disease, ECL-Scan Imaging System","lastPublishedDoi":"10.21203/rs.3.rs-373673/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-373673/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eBackground\u003c/p\u003e\u003cp\u003eEmerging infectious disease have brought a huge impact on human society in recent years. The outbreak of Zika virus (ZIKV) in the Americas resulted in a large number of babies born with microcephaly. More seriously, the Coronavirus Disease 2019 (COVID-19) caused the global spreads and immeasurable damages. Thus, the monitoring of highly pathogenic virus is of significance to the prevention and control of emerging infectious disease.\u003c/p\u003e\u003cp\u003eResults\u003c/p\u003e\u003cp\u003eHerein, a dendritic polymer probe-amplified ECL-scan imaging system was constructed to realize trace analysis of viral emerging infectious disease. Dendritic polymer probe was employed as the efficient signal giving-out component that could generate amplified electrochemiluminescence (ECL) signal on the integrated chip. And the signal was detected by a single-photon level charge coupled device-based ECL-scan imaging system. With this strategy,\u003c/p\u003e\u003cp\u003ethe ZIKV in the complex system of blood, urine and saliva were detected. The results indicated that high sensitivity of 50 copies and superior specificity were achieved. Furthermore, this strategy realized highly sensitive detection (10 copies) of S and N protein gene sequence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-Cov2) and spiked pseudovirus samples.\u003c/p\u003e\u003cp\u003eConclusions\u003c/p\u003e\u003cp\u003eThus, the dendritic polymer probe-amplified ECL-scan imaging system suitably met the strict clinical-requirements for trace analysis of emerging virus, and thus has the potential to serve as a paradigm for monitoring of emerging infectious disease.\u003c/p\u003e","manuscriptTitle":"Trace Analysis of Emerging Virus: an Ultrasensitive ECL-Scan Imaging System for Viral Infectious Disease","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2021-04-01 21:12:39","doi":"10.21203/rs.3.rs-373673/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"bed81ca7-ddea-41f1-b6a7-c61dc32ba9ae","owner":[],"postedDate":"April 1st, 2021","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":3358885,"name":"Nanoscience"}],"tags":[],"updatedAt":"2021-05-01T14:46:38+00:00","versionOfRecord":[],"versionCreatedAt":"2021-04-01 21:12:39","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-373673","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-373673","identity":"rs-373673","version":["v1"]},"buildId":"FbvkV6FR0MCFSLy54lSbu","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}