Polymorphism Analysis and Expression Profile of the Estrogen Receptor 2 Gene in Leizhou Black Duck

Polymorphism Analysis and Expression Profile of the Estrogen Receptor 2 Gene in Leizhou Black Duck | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Polymorphism Analysis and Expression Profile of the Estrogen Receptor 2 Gene in Leizhou Black Duck Collins Amponsah Asiamah, Yuanbo Liu, Rungen Ye, Yiting Pan, Li-li Lu, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-838905/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 01 Dec, 2021 Read the published version in Poultry Science → Version 1 posted You are reading this latest preprint version Abstract Background Our previous study on the ovarian transcriptomic analysis in Leizhou black duck revealed that the ESR2 gene was involved in hormone regulation in reproduction and the estrogen signaling pathway related to reproductive performance was enriched. This suggested that ESR2 may have a functional role in the reproductive performance of the Leizhou black duck. Thus, this study aimed at evaluating the polymorphism of the ESR2 gene and its association with egg-laying traits and the distribution pattern of ESR2 mRNA in laying and non-laying Leizhou black ducks. Method In this study, genomic DNA was extracted from blood samples of 101 Leizhou black ducks to identify single nucleotide polymorphisms (SNPs) of the ESR2 gene to elucidate molecular markers highly associated with egg-laying traits. Four (4) each of laying and non-laying Leizhou black ducks were selected to collect different tissues to analyze the ESR2 gene expression. Results A total of 23 SNPs were identified and association analysis of the single SNP sites showed that SNPs g.56805646 T>C and exon 3-20G>A were significantly (P < 0.05) associated with egg weight. Ducks with CT and AG genotypes had significantly higher (P C and exon 320G>A showed that the haplotypes were significantly associated with egg weight where higher egg weight was seen in individuals with H3H4 haplotypes. In the hypothalamus-pituitary-gonadal (HPG) axis, the results of qRT/PCR showed that ESR2 mRNA was significantly (P < 0.05) expressed in the ovaries of both duck groups than in the hypothalamus and pituitary. In the oviduct, ESR2 was significantly (P < 0.05) higher in the infundibulum and magnum of laying and nonlaying ducks respectively. Conclusion This study provides molecular marker for selecting Leizhou black ducks for egg production and provides theoretical knowledge for the study of the related biological functions of the ESR2 gene at the cellular level. Animal Science ESR2 single nucleotide polymorphism egg-laying traits Leizhou black duck Full Text Cite Share Download PDF Status: Published Journal Publication published 01 Dec, 2021 Read the published version in Poultry Science → Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-838905","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research","associatedPublications":[],"authors":[{"id":51089061,"identity":"11eef3c7-4402-4220-9d8e-6effe17cb3ef","order_by":0,"name":"Collins Amponsah Asiamah","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA+0lEQVRIiWNgGAWjYBACCQbmBjCDDYiZGSpAJFQEtxZGZC1nQCQjkVoYwIrbQBQBLZLtjW3SBX/s7PnYTyd+LpxXG83fDtTyo2IbTi3SPAfbpGe2JSe28eRulp657XjujMOMDYw9Z27j1CInkdgmzdvAnMDGkLtBmnfbsdwGoBagCwlo4flTb8/G/3bzb945x3LnE9IiDdbCdpixTSJ3G9C6mtwNhLRI9hxstuZtO57YJvF2mzXPsQO5G4FaDuLzi8Tx5oO3ef5U28v3526+zVNTlzvv/OGDD35U4NYCBCwSSJzDYPIAPvVAwPwBiVNHQPEoGAWjYBSMRAAA42dXYKFCgxIAAAAASUVORK5CYII=","orcid":"https://orcid.org/0000-0001-8799-026X","institution":"Guangdong Ocean University","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"Collins","middleName":"Amponsah","lastName":"Asiamah","suffix":""},{"id":51089062,"identity":"11535e2f-5584-4bf5-9523-d95fe12c4097","order_by":1,"name":"Yuanbo Liu","email":"","orcid":"","institution":"Guangdong Ocean 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PDF\u003c/a\u003e."}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"ESR2, single nucleotide polymorphism, egg-laying traits, Leizhou black duck","lastPublishedDoi":"10.21203/rs.3.rs-838905/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-838905/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eBackground\u003c/p\u003e\u003cp\u003eOur previous study on the ovarian transcriptomic analysis in Leizhou black duck revealed that the ESR2 gene was involved in hormone regulation in reproduction and the estrogen signaling pathway related to reproductive performance was enriched. This suggested that ESR2 may have a functional role in the reproductive performance of the Leizhou black duck. Thus, this study aimed at evaluating the polymorphism of the ESR2 gene and its association with egg-laying traits and the distribution pattern of ESR2 mRNA in laying and non-laying Leizhou black ducks. \u003c/p\u003e\u003cp\u003eMethod\u003c/p\u003e\u003cp\u003eIn this study, genomic DNA was extracted from blood samples of 101 Leizhou black ducks to identify single nucleotide polymorphisms (SNPs) of the ESR2 gene to elucidate molecular markers highly associated with egg-laying traits. Four (4) each of laying and non-laying Leizhou black ducks were selected to collect different tissues to analyze the ESR2 gene expression. \u003c/p\u003e\u003cp\u003eResults\u003c/p\u003e\u003cp\u003eA total of 23 SNPs were identified and association analysis of the single SNP sites showed that SNPs g.56805646 T\u0026gt;C and exon 3-20G\u0026gt;A were significantly (P \u0026lt; 0.05) associated with egg weight. Ducks with CT and AG genotypes had significantly higher (P \u0026lt; 0.05) egg weights than their respective other genotypes. Haplotype association analysis of g.56805646 T\u0026gt;C and exon 320G\u0026gt;A showed that the haplotypes were significantly associated with egg weight where higher egg weight was seen in individuals with H3H4 haplotypes. In the hypothalamus-pituitary-gonadal (HPG) axis, the results of qRT/PCR showed that ESR2 mRNA was significantly (P \u0026lt; 0.05) expressed in the ovaries of both duck groups than in the hypothalamus and pituitary. In the oviduct, ESR2 was significantly (P \u0026lt; 0.05) higher in the infundibulum and magnum of laying and nonlaying ducks respectively. \u003c/p\u003e\u003cp\u003eConclusion\u003c/p\u003e\u003cp\u003eThis study provides molecular marker for selecting Leizhou black ducks for egg production and provides theoretical knowledge for the study of the related biological functions of the ESR2 gene at the cellular level.\u003c/p\u003e","manuscriptTitle":"Polymorphism Analysis and Expression Profile of the Estrogen Receptor 2 Gene in Leizhou Black Duck","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2021-09-14 14:31:26","doi":"10.21203/rs.3.rs-838905/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"b247c722-aaea-4b3e-95c8-d3080d32e745","owner":[],"postedDate":"September 14th, 2021","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[{"id":7166260,"name":"Animal Science"}],"tags":[],"updatedAt":"2021-12-01T08:18:05+00:00","versionOfRecord":{"articleIdentity":"rs-838905","link":"https://doi.org/10.1016/j.psj.2021.101630","journal":{"identity":"poultry-science","isVorOnly":true,"title":"Poultry Science"},"publishedOn":"2021-12-01 08:18:05","publishedOnDateReadable":"December 1st, 2021"},"versionCreatedAt":"2021-09-14 14:31:26","video":"","vorDoi":"10.1016/j.psj.2021.101630","vorDoiUrl":"https://doi.org/10.1016/j.psj.2021.101630","workflowStages":[]},"version":"v1","identity":"rs-838905","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-838905","identity":"rs-838905","version":["v1"]},"buildId":"WrCJVZZCHTDjtuVLN7oU0","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}