The High-quality genome assembly of Lactarius hatsudake

Lactarius hatsudake is a species of Lactarius commonly found in pine forests, is edible with a delicious and nutritious fruiting body and exhibits medicinal properties. It is an ideal natural multi-functional food with bioactive components including fungal polysaccharides, crude fiber, unsaturated fatty acids, nucleic acid derivatives, various amino acids, and vitamins. However, biological and genomic analyses of this mycorrhizal mushroom is sparse, thereby hindering large-scale cultivation. Previously, we isolated and screened L. hatsudake JH5 strains and have been applied our garnered knowledge to the large-scale cultivation of mycorrhizal seedlings. In this study we produced a high-quality genome assembly of L. hatsudake JH5 by combining Illumina paired-end (PE) and PacBio single molecule real-time (SMRT) sequencing which resulted in PacBio SMRT reads of 7.67Gb and Illumina Pair-End (PE) reads of 1560Mb. Based on the distribution of k-mer frequencies, the genome size of this strain was estimated to be 63.84Mb (1.14% heterozygosity). Based on de novo genome assembly, a final genome size was determined to be 73.16 Mb, with scaffold N50 of 223.2 kb and N90 of 54.5 kb, and a GC content of 54.38%. BUSCO assessment showed that genome completeness was 89.0%. The N50 length of the JH5 genome was 43.62% longer than that of the previously published L. hatsudake MG20 genome. This high-quality L. hatsudake genome assembly will facilitate research on the functional genome, molecular breeding, yield enhancement and sustainability of L. hatsudake cultivation.