Analysis of Internal Transcribed Spacer Regions II Gene and Morphology of Paragonimus From Yunnan Province, China

Abstract For a long time, there is no clear-cut to identify some species of paragonimus in Yunnan Province, China. This paper involved the distribution of Paragonimus in Jinping country and Baoshan city, Yunnan province. In this experiment, the metacercariae, excysted metacercariae, eggs, adult worms were obtained from different hosts were observed and measured. Cats have been described as the appropriate definitive host of paragonimus sp., which are closed to P. cheni according to morphology. Especially the ovaries of adult worms are few and has no third branches. With SEM observation, their spines in the surface are single, a sharp-pointed knife or half-moon in shape and the end of a few of spines are bifurcate. While the clustered sequences strains of this study in the ITS2 tree clustered of Yunan is out sider of P. skirjabini complex and have the genetically greater distances than other isolates of the P. skrjabini complex. Therefore, the Paragonimus sp. of this study from Jinping County and Baoshan city are the same subspecies of P. skrjabini complex.