The effect of growth regulators on the formation of Solanum tuberosum L. micro-plants in vitro

Abstract Micro-plants of different varieties of potatoes (Solanum tuberosum L.) of different ripening times: very early ‘VB Venta’, early ‘Goda’, medium ‘VB Meda’ and very late ‘VB Aista’, as well as growth regulators IAR (indole-3-acetic acid) and kinetin (6-furfuryl aminopurine). The length (cm) and weight (g) of the potato micro-plants were evaluated during testing. Microplant explants were grown in modified MS nutrient medium with different amounts of growth regulators. The results of auxin (IAR) studies showed that the highest number of micro-plants grew from the ‘VB Meda’ variety (10.03 ± 0.96 cm), and the lowest number of micro-plants grew from ‘VB Venta’ (6.2 ± 0.75 cm); the explosives of the ‘VB Meda’ variety (0.218 ± 0.09 g) formed the largest mass of micro-plants. At a concentration of 1.75 ml l− 1 auxin, a sudden increase in the length and weight of the micro-plants was observed. The results of experiments using kinetin showed that the length of potato micro-plants of different genotypes is not the same. The longest micro-plants were formed by the ‘VB Meda’ variety (9.03 ± 0.18 cm) and the smallest by ‘VB Venta’ (5.20 ± 0.15 cm) explants. Increases in the length and weight of micro-plants were observed using kinetin at a concentration of 0.8 ml l− 1.