Multidimensional analysis of the effects of drought stress on chlorophyll synthesis in Lonicera japonica leaves

Multidimensional analysis of the effects of drought stress on chlorophyll synthesis in Lonicera japonica leaves | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Multidimensional analysis of the effects of drought stress on chlorophyll synthesis in Lonicera japonica leaves Haizhen Wang, Boxiang Zhang, Caiping Feng, Xia Zhang This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4875887/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background Lonicera japonica is an important medicinal plant, and its drought resistance is often related to the yield and the economic income of farmers. The drought resistance of plants is closely related to the mechanism of chlorophyll synthesis and decomposition. At present, many scientific reports have focused on the effects of drought stress on photosynthesis and chlorophyll content of L. japonica , but have not studied the chlorophyll anabolism of L. japonica from the perspective of molecular mechanism and metabolic level. Results To analyze the response mechanism of chlorophyll synthesis in honeysuckle ( Lonicera japonica ) plants under drought stress and to screen for the key regulatory genes and precursor metabolites associated with chlorophyll synthesis, a control group (CK) and three experimental groups, which were subjected to light stress (L), moderate stress (M), or severe stress (S), were established in this study. The mechanism of chlorophyll synthesis in L. japonica leaves under drought stress was comprehensively analyzed at the physiological, biochemical, transcriptional, and metabolic levels. Physiological and biochemical studies revealed that with increasing drought stress, the total chlorophyll content ( C t ) of L. japonica leaves tended to initially exhibit a slight increase and then a sharp decrease, and the content of most chlorophyll synthesis-related enzymes decreased. A total of 37,735 differential genes (DEGs) were identified by transcriptome analysis, 16 of which were related to chlorophyll synthesis. Metabolomic analysis revealed a total of 2,132 differentially abundant metabolites, 4 of which were precursors of differentially accumulated substances in the chlorophyll synthesis pathway. Conclusions The multidimensional analysis results show that the metabolite Mg-protoporphyrin IX monomethyl ester and the closely related gene HEMA1 may be key precursors of chlorophyll synthesis and key regulators of chlorophyll synthesis in L. japonica during drought stress. However, the mechanism of the influence of HEMA1 and Mg-protoporphyrin IX monomethyl ester on chlorophyll synthesis under drought stress has yet to be explored. This study is highly important for furthering the understanding of drought resistance mechanisms in medicinal plants and for molecular breeding to produce new varieties of honeysuckle with strong drought tolerance. Lonicera japonica Drought stress Chlorophyll synthesis Metabolome Transcriptome Full Text Additional Declarations No competing interests reported. Supplementary Files Supplementarymaterials.zip Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4875887","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":339282124,"identity":"84fce97d-b7d1-4eba-a916-b111a79f112b","order_by":0,"name":"Haizhen Wang","email":"","orcid":"","institution":"Lyuliang University","correspondingAuthor":false,"prefix":"","firstName":"Haizhen","middleName":"","lastName":"Wang","suffix":""},{"id":339282125,"identity":"e7014f84-5adc-45e6-a1b7-3af46e85691d","order_by":1,"name":"Boxiang Zhang","email":"","orcid":"","institution":"Lyuliang University","correspondingAuthor":false,"prefix":"","firstName":"Boxiang","middleName":"","lastName":"Zhang","suffix":""},{"id":339282128,"identity":"b27100bb-d794-4bd8-ab06-84c9febf826b","order_by":2,"name":"Caiping Feng","email":"","orcid":"","institution":"Lyuliang University","correspondingAuthor":false,"prefix":"","firstName":"Caiping","middleName":"","lastName":"Feng","suffix":""},{"id":339282131,"identity":"02ac5575-b33e-4db3-811e-baee9fa168c0","order_by":3,"name":"Xia Zhang","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA1ElEQVRIie3PMQrCMBTG8YRAuwS6xsGeIdBFUPAq7y26iHMHh0AhHV29R8E5JeAUd0ePoLg4qalODsa6OeS/ffB+wyMkFvvDMsaMATnhWcqU3+w7GdQazbGc5YOK9iTSuaI9OltI25eQA0iDmmFjqRakHKNK9yYo6AbAkwS3T+LmqPgSgoQJMJ7wF6HaohJcBkkiUHkisKk6cutBOLfEgJOFZB1RPYhItScl5MLSagS7eaH5IkymNrucr/LOs7pqD6fVeLhOXZi8B913P9zHYrFY7EMPKjFJW997AOMAAAAASUVORK5CYII=","orcid":"","institution":"Lyuliang University","correspondingAuthor":true,"prefix":"","firstName":"Xia","middleName":"","lastName":"Zhang","suffix":""}],"badges":[],"createdAt":"2024-08-07 15:38:23","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4875887/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4875887/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":74696572,"identity":"19d7b13f-fe09-45eb-a528-fc80c2cf1b73","added_by":"auto","created_at":"2025-01-24 20:31:32","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1096392,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4875887/v1_covered_cfb14ab3-b2e3-4bf2-b276-16c56596323a.pdf"},{"id":63921488,"identity":"f2d2b7fa-5668-4125-9c5a-47eddd317a7d","added_by":"auto","created_at":"2024-09-03 19:53:05","extension":"zip","order_by":23,"title":"","display":"","copyAsset":false,"role":"supplement","size":14644989,"visible":true,"origin":"","legend":"","description":"","filename":"Supplementarymaterials.zip","url":"https://assets-eu.researchsquare.com/files/rs-4875887/v1/1d133f40447f33b36bcb2369.zip"}],"financialInterests":"No competing interests reported.","formattedTitle":"Multidimensional analysis of the effects of drought stress on chlorophyll synthesis in Lonicera japonica leaves","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Lonicera japonica, Drought stress, Chlorophyll synthesis, Metabolome, Transcriptome","lastPublishedDoi":"10.21203/rs.3.rs-4875887/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4875887/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e \u003cb\u003eBackground\u003c/b\u003e \u003cem\u003eLonicera japonica\u003c/em\u003e is an important medicinal plant, and its drought resistance is often related to the yield and the economic income of farmers. The drought resistance of plants is closely related to the mechanism of chlorophyll synthesis and decomposition. At present, many scientific reports have focused on the effects of drought stress on photosynthesis and chlorophyll content of \u003cem\u003eL. japonica\u003c/em\u003e, but have not studied the chlorophyll anabolism of \u003cem\u003eL. japonica\u003c/em\u003e from the perspective of molecular mechanism and metabolic level.\u003c/p\u003e \u003cp\u003e \u003cb\u003eResults\u003c/b\u003e To analyze the response mechanism of chlorophyll synthesis in honeysuckle (\u003cem\u003eLonicera japonica\u003c/em\u003e) plants under drought stress and to screen for the key regulatory genes and precursor metabolites associated with chlorophyll synthesis, a control group (CK) and three experimental groups, which were subjected to light stress (L), moderate stress (M), or severe stress (S), were established in this study. The mechanism of chlorophyll synthesis in \u003cem\u003eL. japonica\u003c/em\u003e leaves under drought stress was comprehensively analyzed at the physiological, biochemical, transcriptional, and metabolic levels. Physiological and biochemical studies revealed that with increasing drought stress, the total chlorophyll content (\u003cem\u003eC\u003c/em\u003e\u003csub\u003e\u003cem\u003et\u003c/em\u003e\u003c/sub\u003e) of \u003cem\u003eL. japonica\u003c/em\u003e leaves tended to initially exhibit a slight increase and then a sharp decrease, and the content of most chlorophyll synthesis-related enzymes decreased. A total of 37,735 differential genes (DEGs) were identified by transcriptome analysis, 16 of which were related to chlorophyll synthesis. Metabolomic analysis revealed a total of 2,132 differentially abundant metabolites, 4 of which were precursors of differentially accumulated substances in the chlorophyll synthesis pathway.\u003c/p\u003e \u003cp\u003e \u003cb\u003eConclusions\u003c/b\u003e The multidimensional analysis results show that the metabolite Mg-protoporphyrin IX monomethyl ester and the closely related gene \u003cem\u003eHEMA1\u003c/em\u003e may be key precursors of chlorophyll synthesis and key regulators of chlorophyll synthesis in \u003cem\u003eL. japonica\u003c/em\u003e during drought stress. However, the mechanism of the influence of \u003cem\u003eHEMA1\u003c/em\u003e and Mg-protoporphyrin IX monomethyl ester on chlorophyll synthesis under drought stress has yet to be explored. This study is highly important for furthering the understanding of drought resistance mechanisms in medicinal plants and for molecular breeding to produce new varieties of honeysuckle with strong drought tolerance.\u003c/p\u003e","manuscriptTitle":"Multidimensional analysis of the effects of drought stress on chlorophyll synthesis in Lonicera japonica leaves","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-09-03 19:52:59","doi":"10.21203/rs.3.rs-4875887/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"14ce2a16-df03-4096-93a1-b21aeaf6912f","owner":[],"postedDate":"September 3rd, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2025-01-24T20:23:23+00:00","versionOfRecord":[],"versionCreatedAt":"2024-09-03 19:52:59","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-4875887","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4875887","identity":"rs-4875887","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}