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This preprint reviews the yeast Saccharomyces cerevisiae as a key eukaryotic model organism and microbial chassis, highlighting its rapid growth, tractable genetics, conserved biological pathways with higher eukaryotes, and broad use in molecular biology and biotechnology. It summarizes major contributions such as the 1996 completion of the yeast genome sequence, foundational protein–protein interaction tools like the yeast two-hybrid system, and applications in gene cloning, protein expression, and metabolic engineering, including industrial production of biofuels, biopharmaceuticals, and enzymes. A major caveat explicitly noted is that the work is a preprint and has not been peer reviewed, so the presentation is preliminary. The paper does not explicitly discuss endometriosis or adenomyosis; it was included in the corpus via a keyword match in the upstream search index.
Abstract
Saccharomyces cerevisiae, commonly known as baker's or brewer's yeast, is a single-celled eukaryotic microorganism that has been a cornerstone of molecular biology and industrial biotechnology for decades. Its rapid growth, simple genetics, and status as a eukaryotic model organism make it an invaluable tool for studying fundamental biological processes that are conserved in higher eukaryotes, including humans. Its utility extends beyond the laboratory, as it serves as a workhorse for the production of biopharmaceuticals, industrial enzymes, biofuels, and fine chemicals. The completion of its genome sequence in 1996 marked a major milestone, providing a blueprint for understanding eukaryotic gene organization and function. From pioneering the yeast two-hybrid system for studying protein interactions to serving as a microbial chassis for metabolic engineering, S. cerevisiae has made unparalleled contributions. This review comprehensively examines the role of S. cerevisiae in molecular biology and biotechnology, focusing on key strains, applications in gene cloning, protein expression, and its broad impact on industrial processes, including biofuels and biopharmaceutical production.
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Saccharomyces cerevisiae in Molecular Biology and Biotechnology | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 20 August 2025 V1 Latest version Share on Saccharomyces cerevisiae in Molecular Biology and Biotechnology Author : Shobith Suresh 0000-0001-6336-1418 [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.175571720.05945169/v1 368 views 146 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Saccharomyces cerevisiae, commonly known as baker's or brewer's yeast, is a single-celled eukaryotic microorganism that has been a cornerstone of molecular biology and industrial biotechnology for decades. Its rapid growth, simple genetics, and status as a eukaryotic model organism make it an invaluable tool for studying fundamental biological processes that are conserved in higher eukaryotes, including humans. Its utility extends beyond the laboratory, as it serves as a workhorse for the production of biopharmaceuticals, industrial enzymes, biofuels, and fine chemicals. The completion of its genome sequence in 1996 marked a major milestone, providing a blueprint for understanding eukaryotic gene organization and function. From pioneering the yeast two-hybrid system for studying protein interactions to serving as a microbial chassis for metabolic engineering, S. cerevisiae has made unparalleled contributions. This review comprehensively examines the role of S. cerevisiae in molecular biology and biotechnology, focusing on key strains, applications in gene cloning, protein expression, and its broad impact on industrial processes, including biofuels and biopharmaceutical production. Supplementary Material File (yeast.pdf) Download 260.01 KB Information & Authors Information Version history V1 Version 1 20 August 2025 Copyright This work is licensed under a Creative Commons Attribution 4.0 International License Keywords biofuels biopharmaceuticals biotechnology eukaryotic chassis metabolic engineering model organism molecular biology protein expression saccharomyces cerevisiae yeast Authors Affiliations Shobith Suresh 0000-0001-6336-1418 [email protected] Jawaharlal Nehru Centre for Advanced Scientific Research View all articles by this author Metrics & Citations Metrics Article Usage 368 views 146 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Shobith Suresh. Saccharomyces cerevisiae in Molecular Biology and Biotechnology. Authorea . 20 August 2025. DOI: https://doi.org/10.22541/au.175571720.05945169/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. 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