Hyaluronic Acid Modified with Bisphosphonic Groups and Peptides as Organic Coating for Titanium Implants

Hyaluronic Acid Modified with Bisphosphonic Groups and Peptides as Organic Coating for Titanium Implants | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Hyaluronic Acid Modified with Bisphosphonic Groups and Peptides as Organic Coating for Titanium Implants Lyudmila V. Parfenova, Zulfiya R. Galimshina, Guzel U. Gil’fanova, and 10 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6621605/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract The work presents the results on the development of organic coatings based on conjugates of hyaluronic acid with bisphosphonates of amino acids and oligopeptides – KRGD or the osteogenic growth peptide (OGP) – for modifying the metal surface with a porous inorganic sublayer obtained through plasma-electrolytic oxidation (PEO). Derivatives of hyaluronic acid (HYA) were synthesized via a Michael reaction involving maleimides of γ-aminobutyric acid bisphosphonate and oligopeptides, as well as SH-functionalized HYA. The conjugates were introduced into the pores of PEO coating on coarse-grained Ti (Grade 2) by immersing the metal samples in the solutions. The presence of organic molecules in the PEO sublayer was confirmed by X-ray photoelectron spectroscopy (XPS). The immobilization of organic molecules on the metal surface led to an increase in its hydrophilicity. Further, studies were carried out on human dermal fibroblasts (hDF) and human adipose-derived mesenchymal stem cells (hASC) to assess the cytotoxicity of the materials, as well as to evaluate the adhesion and proliferation of cells on the modified surface. Research has demonstrated a reduction in cell adhesion and proliferation on the samples modified with conjugates of HYA with peptides and bisphosphonates, which may be attributed to the high hydrophilicity of the surface. OGP applied to the Ti-PEO sublayer exhibited a significant positive effect on hASC adhesion and proliferation. implants plasma electrolytic oxidation bisphosphonates RGD peptide osteogenic growth peptide hyaluronic acid Full Text Additional Declarations The authors declare no competing interests. Supplementary Files Supplementaryinformation.pdf Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6621605","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":453863534,"identity":"61abc781-fe09-40b3-8d5d-ec0122cfeb93","order_by":0,"name":"Lyudmila V. 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