Development of a Quantum Dot–Based Fluorescent Immunochromatographic Assay for Rapid Detection of Antibodies Against Varicella-Zoster Virus gL Protein

Development of a Quantum Dot–Based Fluorescent Immunochromatographic Assay for Rapid Detection of Antibodies Against Varicella-Zoster Virus gL Protein | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Development of a Quantum Dot–Based Fluorescent Immunochromatographic Assay for Rapid Detection of Antibodies Against Varicella-Zoster Virus gL Protein Fengyuan Wang, Jingming Zhou, Hongliang Liu, Enping Liu, Xifang Zhu, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9617943/v1 This work is licensed under a CC BY 4.0 License Status: Under Revision Version 1 posted 22 You are reading this latest preprint version Abstract Varicella-Zoster Virus (VZV) infection remains a globally prevalent disease, posing serious health risks, particularly to the elderly and immunocompromised populations. Traditional serological detection methods are often time-consuming and operationally complex, making them unsuitable for the need for rapid screening in primary healthcare settings. Quantum dot-based fluorescent immunochromatographic technology, with its high sensitivity, strong photostability, and operational simplicity, provides a promising approach for the rapid detection of VZV antibodies. In this study, we expressed and purified the recombinant VZV gL protein, screened high-affinity monoclonal antibodies, and constructed a quantum dot-labeled immunochromatographic test strip. Validation using 22 clinical samples demonstrated that the method exhibits high specificity and sensitivity, with a detection limit of 1:10⁶. The concordance rate with a commercial ELISA kit was 95.5%, offering a reliable technical tool for the rapid diagnosis of VZV infection and assessment of immune status. VZV gL protein monoclonal antibody quantum dots fluorescent immunochromatography Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Under Revision Version 1 posted Editorial decision: Revision requested 19 May, 2026 Reviews received at journal 18 May, 2026 Reviews received at journal 17 May, 2026 Reviews received at journal 16 May, 2026 Reviews received at journal 15 May, 2026 Reviews received at journal 13 May, 2026 Reviews received at journal 11 May, 2026 Reviewers agreed at journal 10 May, 2026 Reviewers agreed at journal 10 May, 2026 Reviews received at journal 10 May, 2026 Reviewers agreed at journal 10 May, 2026 Reviewers agreed at journal 09 May, 2026 Reviewers agreed at journal 08 May, 2026 Reviewers agreed at journal 08 May, 2026 Reviewers agreed at journal 08 May, 2026 Reviewers agreed at journal 08 May, 2026 Reviewers agreed at journal 08 May, 2026 Reviewers agreed at journal 08 May, 2026 Reviewers invited by journal 08 May, 2026 Editor assigned by journal 06 May, 2026 Submission checks completed at journal 06 May, 2026 First submitted to journal 05 May, 2026 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-9617943","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":641106546,"identity":"e56eff2c-f931-46cd-b53a-750736c79cea","order_by":0,"name":"Fengyuan Wang","email":"","orcid":"","institution":"Zhengzhou University","correspondingAuthor":false,"prefix":"","firstName":"Fengyuan","middleName":"","lastName":"Wang","suffix":""},{"id":641106547,"identity":"ba0fe84f-b892-43d3-a6a1-27f71459fb36","order_by":1,"name":"Jingming Zhou","email":"","orcid":"","institution":"Zhengzhou 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