Pectobacterium versatile β-lactamase, a common good of the soft rot Pectobacteriaceae species complex

Pectobacterium versatile β-lactamase, a common good of the soft rot Pectobacteriaceae species complex | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 11 February 2025 V1 Latest version Share on Pectobacterium versatile β-lactamase, a common good of the soft rot Pectobacteriaceae species complex Authors : Camille Lorang , Pierre-Yves Canto , Erwan Gueguen , Jacques Pedron , and Marie-Anne Barny 0000-0002-2005-1388 [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.173927596.61128073/v1 183 views 79 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Little is known about the role of antibiotics and associated resistance in microbial ecosystems in the absence of clinical antibiotic pressure. The Soft Rot Pectobacteriaceae (SRP) species complex, which comprises 37 bacterial species that are collectively responsible for the severe rotting of many crops, is an interesting model to analyse the role of β-lactam and β-lactamases in natural ecosystems. In particular, within this complex, most Pectobacterium versatile strains harbour a β-lactamase called BlaPEC-1. The aim of our work was to analyse the role of BlaPEC-1 during infection. To this end, two bla PEC-1-deleted strains were constructed and compared with their wild-type counterparts in vitro and in potato tuber infections. In vitro , the BlaPEC-1 β-lactamase enables P. versatile to resist ampicillin or carbapenem produced by Pectobacterium brasiliense . In mono-infections on potato tubers, bla PEC-1-deleted strains were unaffected in terms of virulence, fitness or association with commensal bacteria. In mixed infections, the BlaPEC-1 β-lactamase proved necessary for the coexistence of P. versatile with the carbapenem-producing strain, and also for the protection of carbapenem-sensitive strains both in vitro and in planta . Interestingly, in planta protection was observed even if the bla PEC-1 gene was repressed and bacteria expressing BlaPEC-1 were in the minority within the symptom. These results indicate that BlaPEC-1 exerts a true β-lactamase function during the infection process and acts as a public good of the SRP species complex. Finally, our results highlight the important role of β-lactamase in maintaining of strains diversity in natural ecosystem. Supplementary Material File (soumission blapec.pdf) Download 4.40 MB Information & Authors Information Version history V1 Version 1 11 February 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords bacteria behavior/social evolution disease biology microbial biology population dynamics Authors Affiliations Camille Lorang Sorbonne Universite View all articles by this author Pierre-Yves Canto Sorbonne Universite View all articles by this author Erwan Gueguen Microbiologie Adaptation et Pathogenie View all articles by this author Jacques Pedron Sorbonne Universite View all articles by this author Marie-Anne Barny 0000-0002-2005-1388 [email protected] Sorbonne Universite View all articles by this author Metrics & Citations Metrics Article Usage 183 views 79 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Camille Lorang, Pierre-Yves Canto, Erwan Gueguen, et al. Pectobacterium versatile β-lactamase, a common good of the soft rot Pectobacteriaceae species complex. Authorea . 11 February 2025. DOI: https://doi.org/10.22541/au.173927596.61128073/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . Format Please select one from the list RIS (ProCite, Reference Manager) EndNote BibTex Medlars RefWorks Direct import Tips for downloading citations document.getElementById('citMgrHelpLink').addEventListener('click', function() { popupHelp(this.href); return false; }); $(".js__slcInclude").on("change", function(e){ if ($(this).val() == 'refworks') $('#direct').prop("checked", false); $('#direct').prop("disabled", ($(this).val() == 'refworks')); }); View Options View options PDF View PDF Figures Tables Media Share Share Share article link Copy Link Copied! Copying failed. 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