Analysis of serum CircRNA in related to gastric cancer

Gastric cancer is one of the most common malignant tumors of the digestive tract and one of the leading causes of death in patients with malignant tumors worldwide. In recent years, with the deepening of circRNA research, more and more evidence indicates that circRNA plays an important role in the occurrence and development of human malignant tumors. This study firstly conducted a retrospective analysis of the case data of gastric cancer patients who were treated at the Wuwei Cancer Hospital between 2015 and 2017. Subsequently, 18 cases of primary gastric cancer patients and 9 healthy people in Wuwei, Gansu Province were used as controls. The high-throughput microarray technology was used to screen the circRNA expression profiles of healthy and gastric cancer patients, and the expression was expressed by bioinformatics methods. Differential circRNA was used for gene ontology (GO) and KEGG pathway analysis, using its enrichment to predict the relevant biological functions of the differentially expressed circRNA and its involved pathways, and predicting miRNAs interacting with differentially expressed circRNAs, and constructing circRNA-miRNA interaction network. Q-PCR, gene organization microarray and bioinformatics techniques were used to validate candidate differential circRNAs and their linear parental genes and regulatable miRNAs. The results showed that there were 137 circRNAs with significant expression differences (including up-regulation of 67 and down-regulation of 70) in gastric cancer patients, and their differential expression may be related to the occurrence and development of gastric cancer; by GO, KEGG enrichment analysis and Regulating miRNA predictive analysis, the gastric cancer-related GO classification, KEGG Pathway and circRNA-miRNA network were preliminarily obtained, suggesting that differential circRNA may participate in gastric cancer-associated GO classification and KEGG pathway by regulating the expression of parental genes and miRNAs to influence the occurrence and development of gastric cancer. Finally, by using has verified the has_circ_0000437 and its parental genes and regulatable miRNAs, it was found that has_circ_0000437 is highly expressed in gastric cancer patients, and has a certain diagnostic value for the clinical diagnosis of gastric cancer. It may regulate its linear parental gene and The expression of miRNAs affects the development, metastasis and prognosis of clinical gastric cancer.