Comparison of Two Mouse Models of Adenomyosis Established by Sulpiride-Induction and Pituitary Transplantation

Objective The purpose of this study was to compare the adenomyosis models in mice generated by two different methods.Methods Intact 7-week old,never-pregnant female BALB /c mice were randomly divided into 3 groups: control group,sulpiride group and pituitary transplantation group,15 mice in each group.Mice of the sulpiride group were given subcutaneous injections of 800 μg sulpiride per 20 g body weight daily for 5 months.A single pituitary from male mouse was transplanted into the lumen of the right uterus of mice in the pituitary transplantation group.The control group received no treatment.All mice were killed after 5 months and the uteri and ovaries were weighed.The right uterus was examined under a 20-fold microscope.Results 5 months later,compared with the control group,the uterine wet weight,the ratio of wet uterus weight /body weight,morphology of HE stained uterine tissue and disease incidence were significantly increased(P 0.05) in both two model groups.There was no statistical difference in the body weight and the ovarian wet weight(P 0.05).Conclusion There are no significant differences in the evaluation indexes between the sulpiride treatment and pituitary transplantation groups.Both the two methods can be used to successfully establish mouse models of adenomyosis for research of this disease.