Rapid multiplexed qPCR analysis of cerebrospinal fluid and blood for highly prevalent somatic alterations present in central nervous system tumors
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OA: closed
CC-BY-4.0
Abstract
Abstract This protocol describes a qPCR-based assay that is designed to detect twelve potential mutations from clinical specimens that are significant to CNS neoplasms. The assay is optimized for plasma and cerebrospinal fluid (CSF), where both cell-free DNA (cfDNA) and DNA derived from circulating tumor cells (CTCs) can be analyzed.
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Source provenance
- europepmc
- last seen: 2026-05-19T01:45:01.086888+00:00
- unpaywall
- last seen: 2026-05-22T02:00:06.705733+00:00
License: CC-BY-4.0