The exhaustive genome comparison effort. A quarter-century later

A quarter century after the Benner and Gonnet groups began their collaboration in evolutionary bioinformatics, evolution-based functional genomics is a field with considerable scope. Even with the remarkable advances in computing power over this period, the explosion of data derived from genomic and protein sources have required more and more sophisticated approaches be developed and utilized. We describe here new software combined with data organization techniques and illustrate how we are harnessing these to place physiological function of protein sequence data using natural history.