Construction and identification of Rab7-ROSA26 knock-in mice by CRISPR/Cas9 technology

Abstract Background: CRISPR/Cas9 system, a Genome editing technique, can achieve the purpose of gene knockout and gene insertion. Because we want to study the relationship between Rab7 and renal fibrosis in vivo, we should construct Rab7-knock-in mice. Results: We constructed Rab7-knock-in mice by CRISPR/Cas9 technology. Using the identification of PCR, southern blot and sequencing, the detected results were in line with our expectation. Conclusions: We constructed Rab7-knock-in mice successful, which can be provided for studying the relationship between Rab7 and renal fibrosis.