Bone Engineering Fluffy poly Lactide-co-glycolide/hydroxyapatite Composite Scaffold with Bone Marrow Mesenchymal Stem Cells: A Promising Strategy for Bone Defects Repair

Treatment of bone defects remains crucial challenge for successful bone healing, which arouses great interests in designing and fabricating ideal biomaterials. In this regard, additive manufacturing with altered properties and characteristics of polymers was introduced as a promising approach for bone defects. The present study focuses on developing a novel fluffy scaffold of poly Lactide-co-glycolide (PLGA) composites with hydroxyapatite (HA) scaffold used in bone defect repair in rabbits. This fluffy PLGA/HA composite scaffold was fabricated by using multi-electro-spinning combined with biomineralization technology. In vitro analysis of human bone marrow mesenchymal stem cells (BMSCs) seeded onto fluffy PLGA/HA composite scaffold showed their ability to adhere, proliferate and cell viability. The micro-CT and histomorphological analysis showed higher mineralized tissue production in rabbit model treated with fluffy PLGA/HA composite scaffold. The obtained results proved a promising strategy to construct fluffy PLGA/HA composite scaffolds used in bone defects.