Simultaneously Subtyping Influenza A Virus into H1, H3, N1 and N2 using one pot quadruplex RT-qPCR assay
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CC-BY-4.0
Abstract
Abstract In the current study, an one pot quadruplex RT-qPCR assay was established and evaluated. The assay’s limit of detection could reach as low as 101 copies/reaction, with good repeatability profile and no cross-reaction with other respiratory pathogens. During clinical evaluation both by blinded samples and real clinical samples, the assay exhibited a 100% coincidence rate with individual commercial RT-qPCR assays. To the best of our knowledge, this is the first report on the simultaneous subtyping influenza A virus into H1, H3, N1, and N2 by one pot quadruplex RT-qPCR assay, which could improve the preparedness for future influenza outbreaks.
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Source provenance
- europepmc
- last seen: 2026-05-19T01:45:01.086888+00:00
- unpaywall
- last seen: 2026-05-22T02:00:06.705733+00:00
License: CC-BY-4.0