A new method for the conservation of ancient colored paintings on ramie textiles

A new method for the conservation of ancient colored paintings on ramie textiles | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research article A new method for the conservation of ancient colored paintings on ramie textiles Jiaojiao Liu, Yuhu Li, Daodao Hu, Huiping Xing, Xiaolian Cao, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-76692/v2 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 31 Jan, 2021 Read the published version in npj Heritage Science → Version 2 posted 4 You are reading this latest preprint version Show more versions Abstract Textiles are valuable cultural heritage items that are susceptible to several degradation processes due to their sensitive nature, such as the case of ancient ma colored-paintings. Therefore, it is important to take measures to protect the precious ma artifacts. Generally, ″ma″ includes ramie, hemp, flax, oil flax, kenaf, jute, and so on. In this paper, an examination and analysis of a painted ma textile were the first step in proposing an appropriate conservation treatment. Standard fiber and light microscopy were used to identify the fiber type of the painted ma textile. Moreover, custom-made reinforcement materials and technology were introduced with the principles of compatibility, durability and reversibility. The properties of tensile strength, aging resistance and color alteration of the new material to be added were studied before and after dry heat aging, wet heat aging and UV light aging. After systematic examination and evaluation of the painted ma textile and reinforcement materials, the optimal conservation treatment was established, and exhibition method was established. Our work presents a new method for the conservation of ancient Chinese painted ramie textiles that would promote the protection of these valuable artifacts. Cultural Studies Art History Painted textiles ramie fiber conservation methods reinforcement cultural relic. Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Figure 9 Figure 10 1. Introduction Textiles in all forms are an essential part of human civilization [1]. The raw materials that have been used for textiles include cotton, ma, silk, wool, goat hair, and so on. Previous reports mainly focus on the degradation and ageing of linen, wool and silk textiles [2-5], the preservation environment of archaeological textiles [6, 7], the analysis and identification of textile components [8-13], the fungal contamination and splicing technology of textile objects [14, 15]. After the first conference on textile conservation held by IIC in The Netherlands in 1964 [16], certain textile conservation methods have been developed, including disinfecting of textile objects [17], cleaning [18], mounting [19], and stitching techniques [20]. It is well known that ma textiles have been used for making uniforms, ropes, paintings, and scriptures. Among them, painted ma textiles are characterized by the flexibility, draping quality, heterogeneity, and multiple layers, making them complex objects to conserve. Ma textiles are made of ma including ramie, hemp, flax, oil flax, kenaf, jute, and so on [21]. China has a history of more than five thousand years and since the Liao and Jin era are significant periods in ancient China, many ma-colored paintings from Buddhist culture and history have been preserved (Fig. 1). These works provide an important reference to understand the historical prosperity, decline and nature of Buddhist culture in the Chinese Liao and Jin era. Unfortunately, all textiles, including ma textiles in museums are exposed to many risks such as uncontrolled relative humidity (RH) and temperature, light and air pollution, and non-standard storage and display methods [22, 23]. The ma fibers are hygroscopic, thus they could react with water, carbon dioxide, and also sulfur dioxide in the air leading to deterioration of the fibers [24]. In addition, the deposition of dust and microorganisms on the surface accelerates chemical reactions that lead to loss of tensile strength and pliability [25]. As a result, the ornamental value of ma fabric is affected. As far as we know, there are few studies on conservation methods of painted ma textiles. Previously, researchers [26, 27] reported some analytical techniques for the identification and characterization of textile materials, the categorization of painted textile, and a collaborative approach between textile and paintings conservators on lining and mounting painted textiles. Fig.1 The painted ma textile object dates to Chinese Liao and Jin era (160×30 cm). Good conservation policy for painted ma textiles should not be only about protection against the risks caused by environmental factors, but also about the use of appropriate conservation methods, required by scientific research. In this article, the selection of reinforcement materials was the first step for these conservation methods. Reinforcement materials should possess an appropriate structure, weight, and fiber to provide adequate support to the ma textiles [28]. Thereby, the type of fibers and extent of damage were studied through different ways of investigation. Before the identification on the fiber type of the original object, painted ma textile was used. After it is completed, painted ramie textile, ramie fiber or ramie net was used. Then, based on the principles of compatibility, durability and reversibility, the new reinforcement materials and techniques were decided. Finally, the conservation methods of the Chinese painted ramie textiles were developed, including realignment of the distorted weave, consolidation of the painting layer, wet cleaning and stain removal, fine realignment of the fibers, reinforcement of the ramie net and in-fill of areas of loss. This work aimed to propose conservation methods for Chinese painted ramie textiles, which would enrich conservation scientist’s information about the conditions of conservation and physical chemical properties of reinforcement materials [29]. 2. Experimental 2.1 Historical context As shown in Fig. 1, there is a colored ma drawing: a pair of Bodhisattva banner-hangings. The banner hand on the left and right of the banner body and the banner foot below have been lost, and both have height of 160 cm and width of 30 cm. The body of this pair of banner-hanging Bodhisattvas showed the image of Bodhisattvas in Early Indian Buddhism, and it is a rare Buddha painting made on ramie fiber. The painting shows light and dark expressive methods, and the painting style has characteristics that integrate many artistic features of India, Greece and the Western Regions of China. This pair of the colored ramie drawing Bodhisattva banner-hangings originates from the Western Regions of China during the Northern and Southern Dynasties [30]. As shown in Fig. 2, there were many signs of damage on the object, such as many missing areas and weft yarn. Fig.2 The types of damage of the ma textile colored painting (a): There are mainly two signs of damage on this object: missing areas (b); missing weft yarn (c). 2.2 Characterization of ma fiber in the painted textile Based on the compatibility principle of reinforcement materials, this experiment applied one analysis method to identify the fiber type of colored painting of ma textile. Preparation of Herzberg coloring agent and samples has been reported [31, 32]. Light microscopy (LM) was used to observe and compare the fiber morphology of ma fiber from painted textile and standard fiber. 2.3 Evaluation of reinforcement material The Xi’an Yuanhaohuazang Museum has collected the colored ramie paintings of the Chinese Liao and Jin era, and samples were taken from the fragments of the degraded colored ramie paintings. The reinforcement material was prepared with the ramie net. Polyvinyl alcohol 217 (PVA-217), ramie fiber and water-based fluororesin were purchased from a commercial company and used as received. 2.3. 1 Accelerated aging of reinforcement material Three general aging methods were employed for the accelerated aging of the reinforcement material, and three parallel experiments were carried out for each group of aging methods. Each sample was aged for 3, 5, and 7 days. Heating textiles or paper for 72 h (3 days) at 100 °C is equivalent of approximately 25 years of natural aging under normal condition [16]. One aging method was dry heat aging, the prepared ramie net and PVA film were put in a blast drying aging box at 105 °C. The second method was wet heat aging, the prepared ramie net and PVA film were put in a constant damp heat aging test box at a temperature of 80 °C and a relative humidity of 65%. The third method was ultraviolet light aging, an ultraviolet weather resistant test box was to age the ramie net and PVA film, the temperature of the aging box was set at 25 °C, the power of the ultraviolet lamp was 60 W, and the vertical distance between the sample and the ultraviolet lamp was 5 cm. 2.3.2 Tensile strength of reinforcement material before and after aging The QT-1136 universal material testing machine was used to test the tensile strength of the ramie net before and after aging for 5 days. The samples were cut into long strips of about 15 mm×5 mm, then a universal material testing machine was used to measure the load when the ramie net was broken. Each sample was measured 10 times to get the average value of the load. The tensile strength was calculated according to the formula: S=F/Lw, where F was the load at break, and Lw was the width of the sample. The X-RiteVS-450 spectrophotometer was applied to test the chromatic aberration ∆E* of the reinforcement materials before and after aging for 3, 5, and 7 days, and each sample was measured 5 times to get the average value. Four layers of ramie net were superimposed to perform the test. The larger the value of ∆E*, the greater the color change of the sample. 2.4 Conservation process The Chinese Liao and Jin era dates back more than a thousand years, and as such many colored paintings of ma fabrics have been buried underground for years, and they are damaged, severely affecting their viewing and exhibition in museums. Certain conservation, such as realignment of the distorted weave, consolidation of the painting layer, wet cleaning and stain removal, fine realignment of the fibers, reinforcement of the ramie net and in-fill of areas of loss. 3. Results And Discussion 3.1 Fiber analysis of ma textile sample LM was used to identify the fiber type of ma textile sample. As shown in Fig. 3a and 3b, the morphology of standard ramie fiber is relatively thick (the average width of the fiber is 30-60 μm), one part of the fiber is shaped like a circular tube, and another part of the fiber is shaped like a flat long ribbon. It shows clear longitudinal stripes, obvious horizontal knots on the fiber wall, and a wine-red color [33, 34]. Careful morphology comparison between standard ramie fiber and fiber of painted ma textile sample, showed that the painted ma textile sample displays the same fiber morphology and color with standard ramie fiber. Therefore, it is deduced that the fiber type of ma textile sample is ramie fiber. Fig.3 Fiber analysis of the painted ma textile: fiber morphology of ramie (a, LM×200; b, LM×400), fiber morphology of painted ma textile (c, LM×200; d, LM×400). 3.2 Preparation of reinforcement material According to the fiber identification result and the principle of compatibility, the reinforcement material was prepared with a ramie net and PVA-217 adhesive. As shown in Fig. 4(a), the ramie thread with a diameter of 0.8~1 mm was woven as a net with a mesh of 3 mm×3 mm. Fig. 4(b) shows that the 5% concentration of the PVA-217 aqueous solution was prepared as the adhesive, and a spray gun was used to evenly spray the adhesive on the ramie net. The distance between the gun head and the ramie net is 3 cm, the spray pressure of the spray gun is 0.5 MPa~0.8 MPa, and the coating thickness of the adhesive is 15~20 μm. Finally, the sprayed ramie net was dried naturally, and cut out of the weaving frame. Fig.4 Preparation of reinforcement net: spin a web on a webbing machine net (a), spray water-soluble adhesive on the woven net (b). 3.3 Durability evaluations of reinforcement material before and after aging Conservation professional groups always employ reinforcement materials with good durability to carry out conservation methods. As the addition to the support fabric will be a permanent intervention to stabilize weakened areas and safely transport the painted ma textile, the durability of the reinforcement material should be evaluated before using it. In this work, the reinforcement materials were evaluated with three kinds of general accelerated aging: dry heat, wet heat, and ultraviolet light. As shown in Table 4, the retention rate of tensile strength of the ramie net after dry heat aging for 5 days and wet heat aging for 5 days is above 80%, and the retention rate of tensile strength after UV light aging for 5 days is above 90%. These aging results show that the ramie net as the reinforcement material possesses good durability for the support and protection of the painted ramie textile. Generally, the durability of the reinforcement material should not be higher than that of the original object, as that would put a strain and cause mechanical stress to the fragile object. Our reinforced ramie net possessed good compatibility with the original object to balance the strain and mechanical stress. Fig. 5 showed that the wet heat aging and ultraviolet light aging had less effect on the color of the reinforcement materials, while the dry heat aging makes more obvious color changes of the PVA film and ramie net. Therefore, it is speculated that the discoloration of the ramie net was caused by the color change of the PVA after aging. The discoloration of the PVA film was caused by the thermal oxidation reaction of the PVA in the dry heat aging box at 105 °C, and some of the hydroxyl groups are oxidized to carbonyl groups, and the reaction mechanism is shown in Fig. 6. Fig. 5 The color alteration data and effect pictures of several ramie nets with PVA-217 adhesive before and after aging several days by using three aging methods. The chromatic aberration data (white numbers) were listed in the effect pictures. With the increasing aging time, the carbonyl content becomes high, and the color of the PVA film gradually changes from colorless to light yellow, and finally turns to yellow. Meanwhile, the PVA film weakens and becomes brittle and hard. Therefore, it is necessary to further improve the color alterations before and after dry heat aging of the reinforcement material, before it can be applied to cultural relics. Fig.6 Reaction mechanism of PVA film during heating aging process. 3.4 R eversibility process Retreat-ability is the main concern of conservators nowadays [35]. The water-soluble adhesive has good reversibility as a bonding agent for ramie net and painted ramie textile, and they are very stable bonding (Fig. 7a). The ramie net could be removed from painted ramie textile (Fig. 7b) after spraying an appropriate amount of ultra-pure water for a few minutes (Fig. 7c). During the above process, the painted ramie textile sample almost has no damage unless adhesive residues, and it had good reprocessing properties (Fig. 7d). Fig. 7 Reversibility process: before (a) and after (d) removing reinforcement material, spray ultra-pure water (b), removal of reinforcement net (c). 3.5 Conservation process and result Through the above scientific evaluation of the reinforcement material, the principles of compatibility, safety, durability, and reversibility for conservation have been considered. Hence, this reinforcement material was applied to the conservation of the painted textile. As shown in Fig. 14(a), this original painted ramie textile was decayed and broken before it was reinforced and restored. In order to preserve the original appearance of the cultural relics, some broken holes, breakages, and breaks were woven and conserved. For safe exhibition and stable preservation of this painted ramie textile, the back of this artwork was strengthened by ramie net, and ramie net was used as the supporting material, stabilizing weakened areas of the painted ramie textile for safe transport. On the other hand, supporting material was also used as a skeleton to stitch the broken parts and helped to fill the missing areas. Overall, the conservation treatment was successful (Fig. 14b). Reinforcement technology of the painted ramie textile in this paper would provide an important reference for the conservation of decayed and extensively damaged textiles. As shown in Fig. 8-13, the conservation process showed down as the following: (1) Realignment of the distorted weave and mechanical cleaning: The painted ramie textile was laid on a table and use tweezers to arrange the damaged fibers on the edge one by one (Fig. 8a). The mechanical cleaning was done by fine and soft wool brush to remove loose dust and dirt (Fig. 8b). Fig. 8 Using tweezers (a) and soft wool brush (b) to arrange the damaged fibers on the edge one by one. (2) Consolidation of pigment in the colored painting: Because the objects would inevitably get into contact with water during the conservation process, in order to prevent loss of pigment particles, we performed the consolidation with 8% concentration of aqueous fluorine ethanol and water solution (Fig. 9a). Absorbent cotton swabs were dipped into an appropriate amount of water-based fluororesin solution, and then it was evenly applied on the colored painting (Fig. 9b), and left to air dry at room temperature. Fig. 9 Consolidation process of pigment in the painted ramie textile. (3) Wet cleaning and stain removal: We added ultra-pure water into the ultra-fine atomization sprayer, and sprayed slowly on the ramie fabric. With the spray, the ultra-pure water would be flowed out of the backing paper until the liquid flowing out was relatively clean (Fig. 10). Then we rolled it out with a rolled towel to make the objects flat on the tabletop. Fig. 10 Wet cleaning and stain removal (4) Fine realignment on the back of the painted ramie textile: The separated and warped threads were arranged in their original place with fine tweezers (Fig. 11). Because some of the weft threads were missing, the scattered weft threads were arranged in the original place before the reinforcement procedure. Fig. 11 Fine realignment of the warped threads was based on the original texture of the fabric. (5) Reinforcement of the ramie net: We coated the prepared ramie net with PVA-217 adhesive, and attached it to the back of the object and flattened it with a rolled wet towel (Fig. 12a). During this process, PVA-217 adhesive attached to the ramie net would be partly dissolved in water, thereby being sticky enough to adhere and support the object (Fig. 12b), and then left to air dry at room temperature. Fig. 12 Reinforcement process of the ramie net: We attached the prepared ramie net to the back of the object (a) and flattened it with a rolled wet towel (b). (6) In-fill of areas of loss: We used the ramie net as the support where the torn parts were stitched, and the missing areas were woven with aged ramie threads (Fig. 13). Aged ramie was preferred as its mechanical properties would be similar to those of the original object. Fig. 13 The weave process of decayed ramie fabrics: before weaving (a), weaving with ramie threads (b), and after weaving (c). Fig. 14 Effect pictures of decayed ramie fabrics before (a) and after (b) conservation. 3.6 Exhibition and collection After conservation, the painted ramie textile has been stably exhibited for a long time, which is an important mean to evaluate the conservation materials and treatment. Period of time, the painted ramie textile has been monitored for safety and stability since 2016. The results showed no obvious discoloration, and it has been exhibited in the museum under stable temperature and humidity of 23 °C and 50% (Fig. 15), respectively. For the storage, we recommend that it should be unfolded and laid flat if the storage space permits. Fig.15 Exhibition photo of the painted ramie textile in one museum. 4. Conclusion The painted ramie textile reflects the lifestyle and cultural preferences of the people at that time. In this work, a new method was reported for the conservation of ancient colored paintings on ramie textiles. Firstly, the light microscopy observation results showed that the fiber type of the painted ma textile was ramie. Thereby, the ramie net was chosen as the reinforcement material with the principles of compatibility. Then, the reinforcement materials were evaluated with three kinds of artificial accelerated aging. The characterized results of the tensile strength and color alteration indicate that the ramie net as the reinforcement material possesses good durability to support and protect the painted ramie textile. For the reversibility, the reinforced ramie net could be removed from the original object, after spraying an appropriate amount of ultra-pure water. Finally, the optimal conservation methods were implemented. The use of water-based fluororesin helps to improve the stability of color paint. In-fill of areas of loss could achieve good compatibility with the original object to balance the strain and mechanical stress. After conservation, long-term exhibition has also been shown. Overall, after the identification of the fiber type in the painted ma textile and the performance evaluation of the reinforcement materials, the actual conservation methods have successfully applied to the painted ramie textile during the Liao and Jin Dynasties in China. This work provides an important reference for the fiber identification and conservation of ancient painted ramie textiles. Abbreviations LM: light microscopy; RH: relative humidity. Declarations Acknowledgements The authors would like to thank the conservators and archaeologists in Xi'an Yuanhao Huazang Museum, China for their contributions, and also thank Cong Chen for her help in IR test and Xiaolin Shang for providing guidance on the conservation process. Authors’ contributions JL and YL provided support and guidance for this study. JL performed the conservation process of ramie colored drawing and was a major contributor in writing the manuscript. HX and DH carried out literature and examination. XC and JC assisted in sample testing and data analysis. ZJ was involved in the initial concept of the examination. All authors read and approved the final manuscript. Funding The research is financially supported by National Natural Science Foundation of China (51403121), the Key Research and Development Program of Shaanxi Province (2020SF-358, 2017ZDXM-SF-084) and the Fundamental Research Funds for the Central Universities (GK201903054). Availability of data and materials All data are available on request. Competing interests The authors declare that they have no competing interests. References Bergfjord, U. Mannering, K. M. Frei, M. Gleba, A. B. Scharff, I. Skals, J. Heinemeier, M.-L. Nosch, B. Holst, Nettle as a distinct Bronze Age textile plant, Sci. Rep. 2 (2012) 664/1-664/4. Dilillo, A. Restivo, I. Degano, E. Ribechini, M. P. Colombini, GC/MS investigations of the total lipid fraction of wool: A new approach for modeling the ageing processes induced by iron-gallic dyestuffs on historical and archaeological textiles, Microchem. J. 118 (2015) 131-140. Brzozowska, A. Bogdanowicz, P. Szczęsny, U. Zielenkiewicz, A. Laudy, Evaluation of bacterial diversity on historical silk velvet textiles from the Museum of King John III's Palace at Wilanów, Poland, Int. Biodeter. Biodegr. 131 (2018) 78-87. Kavkler, N. Gunde-Cimerman, P. Zalar, A. Demšar, FTIR spectroscopy of biodegraded historical textiles, Polym. Degrad. Stab. 96(4) (2011) 574-580. Han, J. Wanrooij, M. V. Bommel, A. Quye, Characterisation of chemical components for identifying historical Chinese textile dyes by ultra-high performance liquid chromatography–photodiode array–electrospray ionisation mass spectrometer, J. Chromatogr. A 1479 (2017) 87-96. Pelosi, G. Falletta, B. D. Dominicis, P. Baraldi, The painted silk panels of Palazzo Barberini at Rome. The scientific investigation and preservation challenge, Procedia Chem. 8 (2013) 248-257. Serrano, A. Brokerhof, B. Ankersmit, M. V. Bommel, From the bottom of the sea to the display case: A study into the long-term preservation of archaeological maritime silk textiles in controlled atmosphere, J. Cult. Herit. 45 (2020). 91-100 . E. Ahmed, I. F. Tahoun, I. Elkholy, A. B. Shehata, Y. Ziddan, Identification of natural dyes in rare Coptic textile using HPLC-DAD and mass spectroscopy in museum of Faculty of Arts, Alexandria University, Egypt, Dyes Pigm. 145 (2017) 486-492. Ford, R. L. Henderson, C. M. Rayner, R. S. Blackburn, Mild extraction methods using aqueous glucose solution for the analysis of natural dyes in textile artefacts dyed with Dyer’s madder (Rubia tinctorum L.), J. Chromatogr. A 1487 (2017) 36-46. Gulmini, A. Idone, E. Diana, D. Gastaldi, D. Vaudan, M. Aceto, Identification of dyestuffs in historical textiles: strong and weak points of a non-invasive approach, Dyes Pigm. 98(1) (2013) 136-145. Shahid, J. Wertz, I. Degano, M. Aceto, M. I. Khan, A. Quye, Analytical methods for determination of anthraquinone dyes in historical textiles: A review, Anal. Chim. Acta 1083 (2019) 58-87. Kavkler, A. Demšar, Examination of cellulose textile fibres in historical objects by micro-Raman spectroscopy, Spectrochim. Acta A: Mol. Biomol. Spectrosc. 78(2) (2011) 740-746. Al-Sharairi, I. C. A. Sandu, V. Vasilache, I. Sandu, Recognition of natural silk fibers, dyes and metal threads of historical Romanian textile fragments using the multi-analytical techniques approach, Text. Res. J. 90(15-16) (2020) 1671-1688. Kavkler, N. Gunde-Cimerman, P. Zalar, A. Demšar, Fungal contamination of textile objects preserved in Slovene museums and religious institutions, Int. Biodeter. Biodegr. 97 (2015) 51-59. Gleba, S. Harris, The first plant bast fibre technology: identifying splicing in archaeological textiles, Archaeol. Anthrop.l Sci. 11(5) (2019) 2329-2346. Á. Tímár-Balázsy, D. Eastop, Chemical principles of textile conservation . Oxford: Butterworth-Heinemann, Routledge, 1998. G. Tolmacheva, Preliminary report on conservation and studies of the archaeological textiles from TT 23 (season 2018), Египет и сопредельные страны 2 (2018) 1-18. Aslanidou, C. Tsioptsias, C. Panayiotou, A novel approach for textile cleaning based on supercritical CO 2 and Pickering emulsions, J. Supercrit. Fluids 76 (2013) 83-93. L. Chen, K. A. Jakes, D. W. Foreman, Preservation of archaeological textiles through fibre mineralization, J. Archaeol. Sci. 25(10) (1998) 1015-1021. Schön, The mechanical and supporting effect of stitches in textile conservation, University of Gothenburg , Sweden, 2017. Yang, J. Sun, Z. Xue, Production and machining of plant fiber in China, Academic Periodical of Farm Products Processing , 8 (2009) 65-69. Olaru, T. Malutan, C. M. Ursescu, M. Geba, L. Stratulat, Structural changes in hemp fibers following temperature, humidity and UV or gamma-ray radiation exposure, Cell. Chem. Technol. 50(1) (2016) 31-39. Karadag, Some non-destructive and micro-analytical methods for the conservation on textiles from cultural heritage, International Conference on Cultural Heritage and New Technologies , 2014, Vienna, Austria. R. Bresee, General effects of ageing on textiles, Journal of the American Institute for Conservation 25(1) (1986) 39-48. Maria, M. Jerzy, Archaeological textiles–A need for new methods of analysis and reconstruction, Fibres Text. East. Eur. 15(5-6) (2007) 64-65. Smith, F. Lennard, A literature review of analytical techniques for materials characterisation of painted textiles—Part 1: categorising painted textiles, sampling and the use of optical tools, Journal of the Institute of Conservation 40(1) (2017) 64-82. Cruickshank, H. Delaunay, L. Harrison, Painted textiles and canvas paintings: a collaborative approach to lining and mounting, The conservator 30(1) (2007) 5-18. Abdel-Kareem, Y. Zidan, N. Lokma, H. Ahmed, Conservation of a rare painted ancient Egyptian textile object from the Egyptian museum in Cairo, e-PRESERVATION Science Journal 5 (2008) 9-16. Abdel-Kareem, R. Alfaisal, Treatment, conservation and restoration of the Bedouin dyed textiles in the museum of Jordanian heritage, Mediterranean Archaeology and Archaeometry 10(1) (2010) 25-36. J. Li. Research on the appearance reinforcement of Buddhism ma color painting in the Liao and Jin Period. Shaanxi Normal University , Shaanxi, 2018. Li, Technical investigation of ancient Chinese paper based materials with non-destructive chemical methods, Graduate University of Chinese Academy of Sciences , Beijing, 2010. Y. Yang, J. L. Guo, D. C. Gong, Study on the relationship between morphological characteristics of the fibers of ancient paper and papermaking technologies, China Pulp & Paper 30(12) (2011) 32-35. X. Zhao, Research on identification of hemps in fabric, Prog. Text. Sci. Technol. (03) (2011) 60-62. Gao, Y. P. Wang, G. Wang, H. T. Cheng, G. L. Tian, X. S. Gao, Methods to identify several natural plant fibers, Shanghai Text. Sci. Technol. 37(09) (2009) 7-9. M. Viñas, Contemporary theory of conservation , Oxford: Butterworth-Heinemann, Elsevier, 2005. Tables Table 1 The X-ray fluorescence element analysis of sample one. Elements Ca Si Fe K Ba S Ti Cr Zn Cu Content/% 29.75 26.45 20.10 7.44 7.30 4.22 2.70 1.56 0.36 0.12 Table 2 The X-ray fluorescence element analysis of sample two. Elements Mn Ca Cl K Si S Ti Cu Content/% 41.94 24.19 14.21 13.56 4.20 1.42 0.36 0.12 Table 3 Amino acid composition ratio (%) in the sample sizing material. Sample Ala Gly Val Leu Ile Ser Pro Phe Asp Glu Hyp One 5.8 7.3 4.6 6.4 3.7 22.0 2.4 4.2 6.6 21.1 15.8 Two 11.3 20.5 4.7 6.9 4.1 20.2 10.1 3.1 5.5 9.1 4.6 Note: Asp, Ser, Glu, Pro, Gly, Ala, Val, Ile, Leu, Phe, Hyp in the table are respectively the abbreviations of aspartic acid, serine, glutamic acid, proline, glycine, alanine, valine, isoleucine, leucine, phenylalanine and hydroxyproline. Table 4 Tensile strength of ramie net before and after 5 days aging. Aging methods Without aging Dry heat aging Wet heat aging UV light aging Tensile strength /（kN/m） 1.30 1.05 1.08 1.20 Retention rate/% 100 80.67 82.52 91.95 Note: The data in the table are the average of 10 times of parallel tests. Supplementary Files GraphicalAbstract.jpg Cite Share Download PDF Status: Published Journal Publication published 31 Jan, 2021 Read the published version in npj Heritage Science → Version 2 posted Editor assigned by journal 15 Jan, 2021 Editorial decision: Accept 15 Jan, 2021 Submission checks completed at journal 15 Jan, 2021 Editor invited by journal 15 Jan, 2021 You are reading this latest preprint version Show more versions Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-76692","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research article","associatedPublications":[],"authors":[{"id":8871857,"identity":"93780177-da34-4cd2-88cb-d39465d3e612","order_by":0,"name":"Jiaojiao Liu","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA60lEQVRIiWNgGAWjYBACPmYGBoMEBgZmNgYGxgdQQQO8WtiQtDDDlBLQgsyWIE4LO49BwYMaG3Y+9sPHKn/uuJPYwN68TYKh5g4eh/EYGCQcS2Nm40lLu8175lliA8+xMgmGY88IaGE7DPRLjtltxrbDiQ0SOWYSjA2HCWj5B9TC/8as8CdIi/wbIrQktgG1AA1n4AXbwkNIC1uBQWIf0C8Sz5KlgVqM23jSii0SjuHWws9/eJvhj282yfL9yQc/Ah0m289+eOONDzW4tYAsAkVDMoILIhLwaQBG+gMgYYdfzSgYBaNgFIxoAADr/kiG+qJ/IgAAAABJRU5ErkJggg==","orcid":"","institution":"Shaanxi Normal University","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"Jiaojiao","middleName":"","lastName":"Liu","suffix":""},{"id":8871858,"identity":"b7397aa2-ed1c-46fa-8917-9b120779688c","order_by":1,"name":"Yuhu Li","email":"","orcid":"","institution":"Shaanxi Normal University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Yuhu","middleName":"","lastName":"Li","suffix":""},{"id":8871859,"identity":"3bfae74e-6491-4105-84a2-a50a45855cbd","order_by":2,"name":"Daodao Hu","email":"","orcid":"","institution":"Shaanxi Normal University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Daodao","middleName":"","lastName":"Hu","suffix":""},{"id":8871860,"identity":"0af22ea0-2841-4c93-93a7-3aea5ff86819","order_by":3,"name":"Huiping Xing","email":"","orcid":"","institution":"Shaanxi Normal University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Huiping","middleName":"","lastName":"Xing","suffix":""},{"id":8871861,"identity":"b9506484-e562-4816-8730-322626fe3565","order_by":4,"name":"Xiaolian Cao","email":"","orcid":"","institution":"Shaanxi Normal University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Xiaolian","middleName":"","lastName":"Cao","suffix":""},{"id":8871862,"identity":"108add09-ebea-481a-a42d-8b45760e8107","order_by":5,"name":"Jing Cao","email":"","orcid":"","institution":"Shaanxi Normal University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Jing","middleName":"","lastName":"Cao","suffix":""},{"id":8871863,"identity":"48b8db63-44af-4c54-b5eb-9ffb1ba9614d","order_by":6,"name":"Zhihui Jia","email":"","orcid":"","institution":"Shaanxi Normal University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Zhihui","middleName":"","lastName":"Jia","suffix":""}],"badges":[],"createdAt":"2020-09-12 10:58:32","currentVersionCode":2,"declarations":"","doi":"10.21203/rs.3.rs-76692/v2","doiUrl":"https://doi.org/10.21203/rs.3.rs-76692/v2","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1186/s40494-021-00486-4","type":"published","date":"2021-01-31T15:03:15+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":5332594,"identity":"d00a93e2-0562-4096-84ce-1c410fc234ba","added_by":"auto","created_at":"2021-01-28 11:11:35","extension":"jpg","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":35948,"visible":true,"origin":"","legend":"The photo of an ancient linen fabric.","description":"","filename":"Fig1.jpg","url":"https://assets-eu.researchsquare.com/files/rs-76692/v2/c26e619f7ea42d4d5e604103.jpg"},{"id":5332507,"identity":"08d3ab3e-e6d5-4d97-9544-affed32fcfe5","added_by":"auto","created_at":"2021-01-28 11:08:34","extension":"jpg","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":90181,"visible":true,"origin":"","legend":"The morphologies of sample one before (a) and after dyeing (b), sample two before (c) and after dyeing (d).","description":"","filename":"Fig2.jpg","url":"https://assets-eu.researchsquare.com/files/rs-76692/v2/e4c0be4317306da8ed02e9f1.jpg"},{"id":5332508,"identity":"1f33265c-4a35-46da-a1e7-0249224b4c4e","added_by":"auto","created_at":"2021-01-28 11:08:34","extension":"jpg","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":103936,"visible":true,"origin":"","legend":"The SEM morphology (a) and the surface scan element distribution diagram (b) of sample one, the SEM morphology (c) and the surface scan element distribution diagram (d) of sample two.","description":"","filename":"Fig3.jpg","url":"https://assets-eu.researchsquare.com/files/rs-76692/v2/0a5d2e6321ccd77b6b01de8c.jpg"},{"id":5332595,"identity":"de9d7771-793f-4350-b396-ed9c29c58616","added_by":"auto","created_at":"2021-01-28 11:11:35","extension":"jpg","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":31768,"visible":true,"origin":"","legend":"Gas chromatogram of sample one.","description":"","filename":"Fig4.jpg","url":"https://assets-eu.researchsquare.com/files/rs-76692/v2/06a67dcbe16e6a4112fb4c2e.jpg"},{"id":5332679,"identity":"fdb60413-c505-4efd-a54f-94609a5d7b8b","added_by":"auto","created_at":"2021-01-28 11:14:35","extension":"jpg","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":30358,"visible":true,"origin":"","legend":"Gas chromatogram of sample two.","description":"","filename":"Fig5.jpg","url":"https://assets-eu.researchsquare.com/files/rs-76692/v2/c3d7cae8764562129d09d3a8.jpg"},{"id":5332510,"identity":"e0b9caf8-5876-4e56-a072-7c517fe6e31d","added_by":"auto","created_at":"2021-01-28 11:08:34","extension":"jpg","order_by":6,"title":"Figure 6","display":"","copyAsset":false,"role":"figure","size":24360,"visible":true,"origin":"","legend":"Scatter diagram of ratio factor analysis of amino acid composition in samples one and two.","description":"","filename":"Fig6.jpg","url":"https://assets-eu.researchsquare.com/files/rs-76692/v2/0e05c3f5bb53308dc10f2fba.jpg"},{"id":5332598,"identity":"93b18aa7-272b-4449-a4c4-998aecb30cf6","added_by":"auto","created_at":"2021-01-28 11:11:36","extension":"jpg","order_by":7,"title":"Figure 7","display":"","copyAsset":false,"role":"figure","size":83104,"visible":true,"origin":"","legend":"The chromatic aberration data and effect pictures of the reinforcement materials before and after aging several days by using three aging methods. The chromatic aberration data (white numbers) were listed in the effect pictures.","description":"","filename":"Fig7.jpg","url":"https://assets-eu.researchsquare.com/files/rs-76692/v2/72f29a4f4b07715848e3dd22.jpg"},{"id":5332517,"identity":"e895a972-49af-4ad6-abe7-2b44ba109218","added_by":"auto","created_at":"2021-01-28 11:08:35","extension":"jpg","order_by":8,"title":"Figure 8","display":"","copyAsset":false,"role":"figure","size":24002,"visible":true,"origin":"","legend":"Reaction mechanism of PVA film during heat aging process.","description":"","filename":"Fig8.jpg","url":"https://assets-eu.researchsquare.com/files/rs-76692/v2/089794bbe1597bd018ffc96d.jpg"},{"id":5332678,"identity":"60147804-c322-45f0-ac74-06c0fcdb0810","added_by":"auto","created_at":"2021-01-28 11:14:35","extension":"jpg","order_by":9,"title":"Figure 9","display":"","copyAsset":false,"role":"figure","size":58641,"visible":true,"origin":"","legend":"Effect pictures of decayed and rotten hemp fabrics before and after restoration.","description":"","filename":"Fig9.jpg","url":"https://assets-eu.researchsquare.com/files/rs-76692/v2/6c4df7789fc67be30a7d9d66.jpg"},{"id":5332593,"identity":"0f090ba2-b290-4442-b3b0-396a9369de74","added_by":"auto","created_at":"2021-01-28 11:11:35","extension":"jpg","order_by":10,"title":"Figure 10","display":"","copyAsset":false,"role":"figure","size":69499,"visible":true,"origin":"","legend":"The restoration process of decayed and rotten hemp fabrics.","description":"","filename":"Fig10.jpg","url":"https://assets-eu.researchsquare.com/files/rs-76692/v2/6b41a709c4cefef5f5e6fc9b.jpg"},{"id":13652033,"identity":"4c2a0866-6cce-4a72-9c90-b9c6326ddcac","added_by":"auto","created_at":"2021-09-17 09:47:59","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":880273,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-76692/v2/0400d582-b725-4c62-9053-3a149d16fce3.pdf"},{"id":5332592,"identity":"5d7aee08-786c-4d8f-ad90-8fa6944357e6","added_by":"auto","created_at":"2021-01-28 11:11:35","extension":"jpg","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":132910,"visible":true,"origin":"","legend":"","description":"","filename":"GraphicalAbstract.jpg","url":"https://assets-eu.researchsquare.com/files/rs-76692/v2/a2a99d209c441771ec5b1a30.jpg"}],"financialInterests":"","formattedTitle":"A new method for the conservation of ancient colored paintings on ramie textiles","fulltext":[{"header":"1. Introduction","content":"\u003cp\u003eTextiles in all forms are an essential part of human civilization [1]. The raw materials that have been used for textiles include cotton, ma, silk, wool, goat hair, and so on. Previous reports mainly focus on the degradation and ageing of linen, wool and silk textiles [2-5], the preservation environment of archaeological textiles [6, 7], the analysis and identification of textile components [8-13], the fungal contamination and splicing technology of textile objects [14, 15]. After the first conference on textile conservation held by IIC in The Netherlands in 1964 [16], certain textile conservation methods have been developed, including disinfecting of textile objects [17], cleaning [18], mounting [19], and stitching techniques [20].\u003c/p\u003e\n\u003cp\u003eIt is well known that ma textiles have been used for making uniforms, ropes, paintings, and scriptures. Among them, painted ma textiles are characterized by the flexibility, draping quality, heterogeneity, and multiple layers, making them complex objects to conserve. Ma textiles are made of ma including ramie, hemp, flax, oil flax, kenaf, jute, and so on [21]. China has a history of more than five thousand years and since the Liao and Jin era are significant periods in ancient China, many ma-colored paintings from Buddhist culture and history have been preserved (Fig. 1). These works provide an important reference to understand the historical prosperity, decline and nature of Buddhist culture in the Chinese Liao and Jin era. Unfortunately, all textiles, including ma textiles in museums are exposed to many risks such as uncontrolled relative humidity (RH) and temperature, light and air pollution, and non-standard storage and display methods [22, 23]. The ma fibers are hygroscopic, thus they could react with water, carbon dioxide, and also sulfur dioxide in the air leading to deterioration of the fibers [24]. In addition, the deposition of dust and microorganisms on the surface accelerates chemical reactions that lead to loss of tensile strength and pliability [25]. As a result, the ornamental value of ma fabric is affected. As far as we know, there are few studies on conservation methods of painted ma textiles. Previously, researchers [26, 27] reported some analytical techniques for the identification and characterization of textile materials, the categorization of painted textile, and a collaborative approach between textile and paintings conservators on lining and mounting painted textiles.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFig.1\u003c/strong\u003e The painted ma textile object dates to Chinese Liao and Jin era (160\u0026times;30 cm).\u003c/p\u003e\n\u003cp\u003eGood conservation policy for painted ma textiles should not be only about protection against the risks caused by environmental factors, but also about the use of appropriate conservation methods, required by scientific research. In this article, the selection of reinforcement materials was the first step for these conservation methods. Reinforcement materials should possess an appropriate structure, weight, and fiber to provide adequate support to the ma textiles [28]. Thereby, the type of fibers and extent of damage were studied through different ways of investigation. Before the identification on the fiber type of the original object, painted ma textile was used. After it is completed, painted ramie textile, ramie fiber or ramie net was used. Then, based on the principles of compatibility, durability and reversibility, the new reinforcement materials and techniques were decided. Finally, the conservation methods of the Chinese painted ramie textiles were developed, including realignment of the distorted weave, consolidation of the painting layer, wet cleaning and stain removal, fine realignment of the fibers, reinforcement of the ramie net and in-fill of areas of loss. This work aimed to propose conservation methods for Chinese painted ramie textiles, which would enrich conservation scientist\u0026rsquo;s information about the conditions of conservation and physical chemical properties of reinforcement materials [29].\u003c/p\u003e"},{"header":"2. Experimental","content":"\u003cp\u003e\u003cstrong\u003e2.1 Historical context\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAs shown in Fig. 1, there is a colored ma drawing: a pair of Bodhisattva banner-hangings. The banner hand on the left and right of the banner body and the banner foot below have been lost, and both have height of 160 cm and width of 30 cm. The body of this pair of banner-hanging Bodhisattvas showed the image of Bodhisattvas in Early Indian Buddhism, and it is a rare Buddha painting made on ramie fiber. The painting shows light and dark expressive methods, and the painting style has characteristics that integrate many artistic features of India, Greece and the Western Regions of China. This pair of the colored ramie drawing Bodhisattva banner-hangings originates from the Western Regions of China during the Northern and Southern Dynasties [30]. As shown in Fig. 2, there were many signs of damage on the object, such as\u0026nbsp;many\u0026nbsp;missing areas and weft yarn.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFig.2\u003c/strong\u003e The types of damage of the ma textile colored painting (a): There are mainly two signs of damage on this object: missing areas (b); missing weft yarn (c).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.2\u003c/strong\u003e\u003cstrong\u003e Characterization of \u003cem\u003ema\u003c/em\u003e fiber in the painted textile\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eBased on the compatibility principle of reinforcement materials, this experiment applied one analysis method to identify the fiber type of colored painting of ma textile. Preparation of Herzberg coloring agent and samples has been reported [31, 32]. Light microscopy (LM) was used to observe and compare the fiber morphology of ma fiber from painted textile and standard fiber.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.3\u003c/strong\u003e\u003cstrong\u003e Evaluation of reinforcement material\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe Xi\u0026rsquo;an Yuanhaohuazang Museum has collected the colored ramie paintings of the Chinese Liao and Jin era, and samples were taken from the fragments of the degraded colored ramie paintings. The reinforcement material was prepared with the ramie net. Polyvinyl alcohol 217 (PVA-217), ramie fiber and water-based fluororesin were purchased from a commercial company and used as received.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.3.\u003c/strong\u003e\u003cstrong\u003e1 Accelerated aging of \u003c/strong\u003e\u003cstrong\u003ereinforcement material \u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThree general aging methods were employed for the accelerated aging of the reinforcement material, and three parallel experiments were carried out for each group of aging methods. Each sample was aged for 3, 5, and 7 days. Heating textiles or paper for 72 h (3 days) at 100 \u0026deg;C is equivalent of approximately 25 years of natural aging under normal condition [16]. One aging method was dry heat aging, the prepared ramie net and PVA film were put in a blast drying aging box at 105 \u0026deg;C. The second method was wet heat aging, the prepared ramie net and PVA film were put in a constant damp heat aging test box at a temperature of 80 \u0026deg;C and a relative humidity of 65%. The third method was ultraviolet light aging, an ultraviolet weather resistant test box was to age the ramie net and PVA film, the temperature of the aging box was set at 25 \u0026deg;C, the power of the ultraviolet lamp was 60 W, and the vertical distance between the sample and the ultraviolet lamp was 5 cm.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.3.2 Tensile strength of reinforcement material before and after aging \u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe QT-1136 universal material testing machine was used to test the tensile strength of the ramie net before and after aging for 5 days. The samples were cut into long strips of about 15 mm\u0026times;5 mm, then a universal material testing machine was used to measure the load when the ramie net was broken. Each sample was measured 10 times to get the average value of the load. The tensile strength was calculated according to the formula: S=F/Lw, where F was the load at break, and Lw was the width of the sample. The X-RiteVS-450 spectrophotometer was applied to test the chromatic aberration ∆E* of the reinforcement materials before and after aging for 3, 5, and 7 days, and each sample was measured 5 times to get the average value. Four layers of ramie net were superimposed to perform the test. The larger the value of ∆E*, the greater the color change of the sample.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.4 Conservation process\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe Chinese Liao and Jin era dates back more than a thousand years, and as such many colored paintings of ma fabrics have been buried underground for years, and they are damaged, severely affecting their viewing and exhibition in museums. Certain conservation, such as realignment of the distorted weave, consolidation of the painting layer, wet cleaning and stain removal, fine realignment of the fibers, reinforcement of the ramie net and in-fill of areas of loss.\u003c/p\u003e"},{"header":"3. Results And Discussion","content":"\u003cp\u003e\u003cstrong\u003e3.1 Fiber analysis of ma textile sample\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eLM was used to identify the fiber type of ma textile sample. As shown in Fig. 3a and 3b, the morphology of standard ramie fiber is relatively thick (the average width of the fiber is 30-60 \u0026mu;m), one part of the fiber is shaped like a circular tube, and another part of the fiber is shaped like a flat long ribbon. It shows clear longitudinal stripes, obvious horizontal knots on the fiber wall, and a wine-red color [33, 34]. Careful morphology comparison between standard ramie fiber and fiber of painted ma textile sample, showed that the painted ma textile sample displays the same fiber morphology and color with standard ramie fiber. Therefore, it is deduced that the fiber type of ma textile sample is ramie fiber.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFig.3\u003c/strong\u003e Fiber analysis of the painted ma textile: fiber morphology of ramie (a, LM\u0026times;200; b, LM\u0026times;400), fiber morphology of painted ma textile (c, LM\u0026times;200; d, LM\u0026times;400).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e3.2 Preparation of reinforcement material\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAccording to the fiber identification result and the principle of compatibility, the reinforcement material was prepared with a ramie net and PVA-217 adhesive. As shown in Fig. 4(a), the ramie thread with a diameter of 0.8~1 mm was woven as a net with a mesh of 3 mm\u0026times;3 mm. Fig. 4(b) shows that the 5% concentration of the PVA-217 aqueous solution was prepared as the adhesive, and a spray gun was used to evenly spray the adhesive on the ramie net. The distance between the gun head and the ramie net is 3 cm, the spray pressure of the spray gun is 0.5 MPa~0.8 MPa, and the coating thickness of the adhesive is 15~20 \u0026mu;m. Finally, the sprayed ramie net was dried naturally, and cut out of the weaving frame.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFig.4 \u003c/strong\u003ePreparation of reinforcement net: spin a web on a webbing machine net (a), spray water-soluble adhesive on the woven net (b).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e3.3 Durability evaluations of reinforcement material before and after aging\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eConservation professional groups always employ reinforcement materials with good durability to carry out conservation methods. As the addition to the support fabric will be a permanent intervention to stabilize weakened areas and safely transport the painted ma textile, the durability of the reinforcement material should be evaluated before using it. In this work, the reinforcement materials were evaluated with three kinds of general accelerated aging: dry heat, wet heat, and ultraviolet light. As shown in Table 4, the retention rate of tensile strength of the ramie net after dry heat aging for 5 days and wet heat aging for 5 days is above 80%, and the retention rate of tensile strength after UV light aging for 5 days is above 90%. These aging results show that the ramie net as the reinforcement material possesses good durability for the support and protection of the painted ramie textile. Generally, the durability of the reinforcement material should not be higher than that of the original object, as that would put a strain and cause mechanical stress to the fragile object. Our reinforced ramie net possessed good compatibility with the original object to balance the strain and mechanical stress.\u003c/p\u003e\n\u003cp\u003eFig. 5 showed that the wet heat aging and ultraviolet light aging had less effect on the color of the reinforcement materials, while the dry heat aging makes more obvious color changes of the PVA film and ramie net. Therefore, it is speculated that the discoloration of the ramie net was caused by the color change of the PVA after aging. The discoloration of the PVA film was caused by the thermal oxidation reaction of the PVA in the dry heat aging box at 105 \u0026deg;C, and some of the hydroxyl groups are oxidized to carbonyl groups, and the reaction mechanism is shown in Fig. 6.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFig. 5 \u003c/strong\u003eThe color alteration data and effect pictures of several ramie nets with PVA-217 adhesive before and after aging several days by using three aging methods. The chromatic aberration data (white numbers) were listed in the effect pictures.\u003c/p\u003e\n\u003cp\u003eWith the increasing aging time, the carbonyl content becomes high, and the color of the PVA film gradually changes from colorless to light yellow, and finally turns to yellow. Meanwhile, the PVA film weakens and becomes brittle and hard. Therefore, it is necessary to further improve the color alterations before and after dry heat aging of the reinforcement material, before it can be applied to cultural relics.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFig.6 \u003c/strong\u003eReaction mechanism of PVA film during heating aging process.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e3.4 R\u003c/strong\u003e\u003cstrong\u003eeversibility process\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eRetreat-ability is the main concern of conservators nowadays [35]. The water-soluble adhesive has good reversibility as a bonding agent for ramie net and painted ramie textile, and they are very stable bonding (Fig. 7a). The ramie net could be removed from painted ramie textile (Fig. 7b) after spraying an appropriate amount of ultra-pure water for a few minutes (Fig. 7c). During the above process, the painted ramie textile sample almost has no damage unless adhesive residues, and it had good reprocessing properties (Fig. 7d).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFig. 7 \u003c/strong\u003eReversibility process: before (a) and after (d) removing reinforcement material, spray ultra-pure water (b), removal of reinforcement net (c).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e3.5 Conservation process and result\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThrough the above scientific evaluation of the reinforcement material, the principles of compatibility, safety, durability, and reversibility for conservation have been considered. Hence, this reinforcement material was applied to the conservation of the painted textile. As shown in Fig. 14(a), this original painted ramie textile was decayed and broken before it was reinforced and restored. In order to preserve the original appearance of the cultural relics, some broken holes, breakages, and breaks were woven and conserved. For safe exhibition and stable preservation of this painted ramie textile, the back of this artwork was strengthened by ramie net, and ramie net was used as the supporting material, stabilizing weakened areas of the painted ramie textile for safe transport. On the other hand, supporting material was also used as a skeleton to stitch the broken parts and helped to fill the missing areas. Overall, the conservation treatment was successful (Fig. 14b). Reinforcement technology of the painted ramie textile in this paper would provide an important reference for the conservation of decayed and extensively damaged textiles. As shown in Fig. 8-13, the conservation process showed down as the following:\u003c/p\u003e\n\u003cp\u003e(1) Realignment of the distorted weave and mechanical cleaning: The painted ramie textile was laid on a table and use tweezers to arrange the damaged fibers on the edge one by one (Fig. 8a). The mechanical cleaning was done by fine and soft wool brush to remove loose dust and dirt (Fig. 8b).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFig. \u003c/strong\u003e\u003cstrong\u003e8 \u003c/strong\u003eUsing tweezers (a) and soft wool brush (b) to arrange the damaged fibers on the edge one by one.\u003c/p\u003e\n\u003cp\u003e(2) Consolidation of pigment in the colored painting: Because the objects would inevitably get into contact with water during the conservation process, in order to prevent loss of pigment particles, we performed the consolidation with 8% concentration of aqueous fluorine ethanol and water solution (Fig. 9a). Absorbent cotton swabs were dipped into an appropriate amount of water-based fluororesin solution, and then it was evenly applied on the colored painting (Fig. 9b), and left to air dry at room temperature.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFig. \u003c/strong\u003e\u003cstrong\u003e9 \u003c/strong\u003eConsolidation process of pigment in the painted ramie textile.\u003c/p\u003e\n\u003cp\u003e(3) Wet cleaning and stain removal: We added ultra-pure water into the ultra-fine atomization sprayer, and sprayed slowly on the ramie fabric. With the spray, the ultra-pure water would be flowed out of the backing paper until the liquid flowing out was relatively clean (Fig. 10). Then we rolled it out with a rolled towel to make the objects flat on the tabletop.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFig. 10 \u003c/strong\u003eWet cleaning and stain removal\u003c/p\u003e\n\u003cp\u003e(4) Fine realignment on the back of the painted ramie textile: The separated and warped threads were arranged in their original place with fine tweezers (Fig. 11). Because some of the weft threads were missing, the scattered weft threads were arranged in the original place before the reinforcement procedure.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFig. 11 \u003c/strong\u003eFine realignment of the warped threads was based on the original texture of the fabric.\u003c/p\u003e\n\u003cp\u003e(5) Reinforcement of the ramie net: We coated the prepared ramie net with PVA-217 adhesive, and attached it to the back of the object and flattened it with a rolled wet towel (Fig. 12a). During this process, PVA-217 adhesive attached to the ramie net would be partly dissolved in water, thereby being sticky enough to adhere and support the object (Fig. 12b), and then left to air dry at room temperature.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFig. 12 \u003c/strong\u003eReinforcement process of the ramie net: We attached the prepared ramie net to the back of the object (a) and flattened it with a rolled wet towel (b).\u003c/p\u003e\n\u003cp\u003e(6) In-fill of areas of loss: We used the ramie net as the support where the torn parts were stitched, and the missing areas were woven with aged ramie threads (Fig. 13). Aged ramie was preferred as its mechanical properties would be similar to those of the original object.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFig. 13 \u003c/strong\u003eThe weave process of decayed ramie fabrics: before weaving (a), weaving with ramie threads (b), and after weaving (c).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFig. 14\u003c/strong\u003e Effect pictures of decayed ramie fabrics before (a) and after (b) conservation.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003e3.6 Exhibition and collection\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAfter conservation, the painted ramie textile has been stably exhibited for a long time, which is an important mean to evaluate the conservation materials and treatment. Period of time, the painted ramie textile has been monitored for safety and stability since 2016. The results showed no obvious discoloration, and it has been exhibited in the museum under stable temperature and humidity of 23 \u0026deg;C and 50% (Fig. 15), respectively. For the storage, we recommend that it should be unfolded and laid flat if the storage space permits.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFig.15 \u003c/strong\u003eExhibition photo of the painted ramie textile in one museum.\u003c/p\u003e"},{"header":"4. Conclusion","content":"\u003cp\u003eThe painted ramie textile reflects the lifestyle and cultural preferences of the people at that time. In this work, a new method was reported for the conservation of ancient colored paintings on ramie textiles. Firstly, the light microscopy observation results showed that the fiber type of the painted ma textile was ramie. Thereby, the ramie net was chosen as the reinforcement material with the principles of compatibility. Then, the reinforcement materials were evaluated with three kinds of artificial accelerated aging. The characterized results of the tensile strength and color alteration indicate that the ramie net as the reinforcement material possesses good durability to support and protect the painted ramie textile. For the reversibility, the reinforced ramie net could be removed from the original object, after spraying an appropriate amount of ultra-pure water. Finally, the optimal conservation methods were implemented. The use of water-based fluororesin helps to improve the stability of color paint. In-fill of areas of loss could achieve good compatibility with the original object to balance the strain and mechanical stress. After conservation, long-term exhibition has also been shown. Overall, after the identification of the fiber type in the painted ma textile and the performance evaluation of the reinforcement materials, the actual conservation methods have successfully applied to the painted ramie textile during the Liao and Jin Dynasties in China. This work provides an important reference for the fiber identification and conservation of ancient painted ramie textiles.\u003c/p\u003e"},{"header":"Abbreviations","content":"\u003cp\u003eLM: light microscopy; RH: relative humidity.\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eAcknowledgements\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe authors would like to thank the conservators and archaeologists in Xi'an Yuanhao Huazang Museum, China for their contributions, and also thank Cong Chen for her help in IR test and Xiaolin Shang for providing guidance on the conservation process.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAuthors\u0026rsquo; contributions\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eJL and YL provided support and guidance for this study. JL performed the conservation process of ramie colored drawing and was a major contributor in writing the manuscript. HX and DH carried out literature and examination. XC and JC assisted in sample testing and data analysis. ZJ was involved in the initial concept of the examination. All authors read and approved the final manuscript.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFunding\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe research is financially supported by National Natural Science Foundation of China (51403121), the\u0026nbsp;Key\u0026nbsp;Research and\u0026nbsp;Development Program\u0026nbsp;of\u0026nbsp;Shaanxi\u0026nbsp;Province (2020SF-358, 2017ZDXM-SF-084) and the Fundamental Research Funds for the Central Universities (GK201903054).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAvailability of data and materials\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAll data are available on request.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompeting interests\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe authors declare that they have no competing interests.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\n\u003cli\u003eBergfjord, U. Mannering, K. M. Frei, M. Gleba, A. B. Scharff, I. Skals, J. Heinemeier, M.-L. Nosch, B. Holst, Nettle as a distinct Bronze Age textile plant, \u003cem\u003eSci. Rep.\u003c/em\u003e2 (2012) 664/1-664/4.\u003c/li\u003e\n\u003cli\u003eDilillo, A. Restivo, I. Degano, E. Ribechini, M. P. Colombini, GC/MS investigations of the total lipid fraction of wool: A new approach for modeling the ageing processes induced by iron-gallic dyestuffs on historical and archaeological textiles,\u0026nbsp;\u003cem\u003eMicrochem. J. \u003c/em\u003e118 (2015) 131-140.\u003c/li\u003e\n\u003cli\u003eBrzozowska, A. Bogdanowicz, P. Szczęsny, U. Zielenkiewicz, A. Laudy, Evaluation of bacterial diversity on historical silk velvet textiles from the Museum of King John III's Palace at Wilan\u0026oacute;w, Poland, \u003cem\u003eInt. Biodeter. Biodegr.\u003c/em\u003e131 (2018) 78-87.\u003c/li\u003e\n\u003cli\u003eKavkler, N. Gunde-Cimerman, P. Zalar, A. Dem\u0026scaron;ar, FTIR spectroscopy of biodegraded historical textiles,\u0026nbsp;\u003cem\u003ePolym. Degrad. Stab.\u003c/em\u003e96(4) (2011) 574-580.\u003c/li\u003e\n\u003cli\u003eHan, J. Wanrooij, M. V. Bommel, A. Quye,\u0026nbsp; Characterisation of chemical components for identifying historical Chinese textile dyes by ultra-high performance liquid chromatography\u0026ndash;photodiode array\u0026ndash;electrospray ionisation mass spectrometer,\u0026nbsp;\u003cem\u003eJ. Chromatogr. A\u003c/em\u003e1479 (2017) 87-96.\u003c/li\u003e\n\u003cli\u003ePelosi, G. Falletta, B. D. Dominicis, P. Baraldi, The painted silk panels of Palazzo Barberini at Rome. The scientific investigation and preservation challenge, \u003cem\u003eProcedia Chem. \u003c/em\u003e8 (2013) 248-257.\u003c/li\u003e\n\u003cli\u003eSerrano, A. Brokerhof, B. Ankersmit, M. V. Bommel, From the bottom of the sea to the display case: A study into the long-term preservation of archaeological maritime silk textiles in controlled atmosphere,\u0026nbsp;\u003cem\u003eJ. Cult. Herit. \u003c/em\u003e45 (2020). \u003ca href=\"https://doi.org/10.1016/j.culher.2020.04.004\"\u003e91-100\u003c/a\u003e.\u003c/li\u003e\n\u003cli\u003eE. Ahmed, I. F. Tahoun, I. Elkholy, A. B. Shehata, Y. Ziddan, Identification of natural dyes in rare Coptic textile using HPLC-DAD and mass spectroscopy in museum of Faculty of Arts, Alexandria University, Egypt,\u0026nbsp;\u003cem\u003eDyes Pigm. \u003c/em\u003e145 (2017) 486-492.\u003c/li\u003e\n\u003cli\u003eFord, R. L. Henderson, C. M. Rayner, R. S. Blackburn, Mild extraction methods using aqueous glucose solution for the analysis of natural dyes in textile artefacts dyed with Dyer\u0026rsquo;s madder (Rubia tinctorum L.),\u0026nbsp;\u003cem\u003eJ. Chromatogr. A\u003c/em\u003e1487 (2017) 36-46.\u003c/li\u003e\n\u003cli\u003eGulmini, A. Idone, E. Diana, D. Gastaldi, D. Vaudan, M. Aceto, Identification of dyestuffs in historical textiles: strong and weak points of a non-invasive approach,\u0026nbsp;\u003cem\u003eDyes Pigm. \u003c/em\u003e98(1) (2013) 136-145.\u003c/li\u003e\n\u003cli\u003eShahid, J. Wertz, I. Degano, M. Aceto, M. I. Khan, A. Quye, Analytical methods for determination of anthraquinone dyes in historical textiles: A review, \u003cem\u003eAnal. Chim. Acta\u003c/em\u003e1083 (2019) 58-87.\u003c/li\u003e\n\u003cli\u003eKavkler, A. Dem\u0026scaron;ar, Examination of cellulose textile fibres in historical objects by micro-Raman spectroscopy,\u0026nbsp;\u003cem\u003eSpectrochim. Acta A: Mol. Biomol. Spectrosc. \u003c/em\u003e78(2) (2011) 740-746.\u003c/li\u003e\n\u003cli\u003eAl-Sharairi, I. C. A. Sandu, V. Vasilache, I. Sandu, Recognition of natural silk fibers, dyes and metal threads of historical Romanian textile fragments using the multi-analytical techniques approach,\u0026nbsp;\u003cem\u003eText. Res. J.\u003c/em\u003e 90(15-16) (2020) 1671-1688.\u003c/li\u003e\n\u003cli\u003eKavkler, N. Gunde-Cimerman, P. Zalar, A. Dem\u0026scaron;ar, Fungal contamination of textile objects preserved in Slovene museums and religious institutions, \u003cem\u003eInt. Biodeter. Biodegr.\u003c/em\u003e97 (2015) 51-59.\u003c/li\u003e\n\u003cli\u003eGleba, S. Harris, The first plant bast fibre technology: identifying splicing in archaeological textiles, \u003cem\u003eArchaeol. Anthrop.l Sci.\u003c/em\u003e 11(5) (2019) 2329-2346.\u003c/li\u003e\n\u003cli\u003e\u0026Aacute;. T\u0026iacute;m\u0026aacute;r-Bal\u0026aacute;zsy, D. Eastop, \u003cem\u003eChemical principles of textile conservation\u003c/em\u003e. Oxford: Butterworth-Heinemann, Routledge, 1998.\u003c/li\u003e\n\u003cli\u003eG. Tolmacheva, Preliminary report on conservation and studies of the archaeological textiles from TT 23 (season 2018), \u003cem\u003eЕгипет и сопредельные страны\u003c/em\u003e 2 (2018) 1-18.\u003c/li\u003e\n\u003cli\u003eAslanidou, C. Tsioptsias, C. Panayiotou, A novel approach for textile cleaning based on supercritical CO\u003csub\u003e2\u003c/sub\u003e and Pickering emulsions,\u003cem\u003e J. Supercrit. Fluids\u003c/em\u003e76 (2013) 83-93.\u003c/li\u003e\n\u003cli\u003eL. Chen, K. A. Jakes, D. W. Foreman, Preservation of archaeological textiles through fibre mineralization,\u0026nbsp;\u003cem\u003eJ. Archaeol. Sci.\u003c/em\u003e25(10) (1998) 1015-1021.\u003c/li\u003e\n\u003cli\u003eSch\u0026ouml;n, The mechanical and supporting effect of stitches in textile conservation, \u003cem\u003eUniversity of Gothenburg\u003c/em\u003e, Sweden, 2017.\u003c/li\u003e\n\u003cli\u003eYang, J. Sun, Z. Xue, Production and machining of plant fiber in China, \u003cem\u003eAcademic Periodical of Farm Products Processing\u003c/em\u003e, 8 (2009) 65-69.\u003c/li\u003e\n\u003cli\u003eOlaru, T. Malutan, C. M. Ursescu, M. Geba, L. Stratulat, Structural changes in hemp fibers following temperature, humidity and UV or gamma-ray radiation exposure,\u0026nbsp;\u003cem\u003eCell. Chem. Technol.\u003c/em\u003e50(1) (2016) 31-39.\u003c/li\u003e\n\u003cli\u003eKaradag, Some non-destructive and micro-analytical methods for the conservation on textiles from cultural heritage, \u003cem\u003eInternational Conference on Cultural Heritage and New Technologies\u003c/em\u003e, 2014, Vienna, Austria.\u003c/li\u003e\n\u003cli\u003eR. Bresee, General effects of ageing on textiles,\u0026nbsp;\u003cem\u003eJournal of the American Institute for Conservation\u003c/em\u003e25(1) (1986) 39-48.\u003c/li\u003e\n\u003cli\u003eMaria, M. Jerzy, Archaeological textiles\u0026ndash;A need for new methods of analysis and reconstruction, \u003cem\u003eFibres Text. East. Eur.\u003c/em\u003e15(5-6) (2007) 64-65.\u003c/li\u003e\n\u003cli\u003eSmith, F. Lennard, A literature review of analytical techniques for materials characterisation of painted textiles\u0026mdash;Part 1: categorising painted textiles, sampling and the use of optical tools,\u0026nbsp;\u003cem\u003eJournal of the Institute of Conservation\u003c/em\u003e40(1) (2017) 64-82.\u003c/li\u003e\n\u003cli\u003eCruickshank, H. Delaunay, L. Harrison, Painted textiles and canvas paintings: a collaborative approach to lining and mounting,\u0026nbsp;\u003cem\u003eThe conservator\u003c/em\u003e30(1) (2007) 5-18.\u003c/li\u003e\n\u003cli\u003eAbdel-Kareem, Y. Zidan, N. Lokma, H. Ahmed, Conservation of a rare painted ancient Egyptian textile object from the Egyptian museum in Cairo,\u0026nbsp;\u003cem\u003ee-PRESERVATION Science Journal\u003c/em\u003e5 (2008) 9-16.\u003c/li\u003e\n\u003cli\u003eAbdel-Kareem, R. Alfaisal, Treatment, conservation and restoration of the Bedouin dyed textiles in the museum of Jordanian heritage, \u003cem\u003eMediterranean Archaeology and Archaeometry\u003c/em\u003e10(1) (2010) 25-36.\u003c/li\u003e\n\u003cli\u003eJ. Li. Research on the appearance reinforcement of Buddhism \u003cem\u003ema\u003c/em\u003e color painting in the Liao and Jin Period. \u003cem\u003eShaanxi Normal University\u003c/em\u003e, Shaanxi, 2018.\u003c/li\u003e\n\u003cli\u003eLi, Technical investigation of ancient Chinese paper based materials with non-destructive chemical methods, \u003cem\u003eGraduate University of Chinese Academy of Sciences\u003c/em\u003e, Beijing, 2010.\u003c/li\u003e\n\u003cli\u003eY. Yang, J. L. Guo, D. C. Gong, Study on the relationship between morphological characteristics of the fibers of ancient paper and papermaking technologies, \u003cem\u003eChina Pulp \u0026amp; Paper\u003c/em\u003e 30(12) (2011) 32-35.\u003c/li\u003e\n\u003cli\u003eX. Zhao, Research on identification of hemps in fabric, \u003cem\u003eProg. Text. Sci. Technol.\u003c/em\u003e (03) (2011) 60-62.\u003c/li\u003e\n\u003cli\u003eGao, Y. P. Wang, G. Wang, H. T. Cheng, G. L. Tian, X. S. Gao, Methods to identify several natural plant fibers, \u003cem\u003eShanghai Text. Sci. Technol. \u003c/em\u003e37(09) (2009) 7-9.\u003c/li\u003e\n\u003cli\u003eM. Vi\u0026ntilde;as, \u003cem\u003eContemporary theory of conservation\u003c/em\u003e, Oxford: Butterworth-Heinemann, Elsevier, 2005.\u003c/li\u003e\n\u003c/ol\u003e"},{"header":"Tables","content":"\u003ctable border=\"1\" data-mce-selected=\"1\" style=\"outline: black solid 1px; resize: none; color: rgb(0, 0, 0); font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial;\"\u003e\n \u003ccaption\u003e\n \u003cdiv class=\"CaptionNumber\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif; font-size: 15px;\"\u003eTable 1\u003c/span\u003e\u003c/div\u003e\n \u003cdiv class=\"CaptionContent\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eThe X-ray fluorescence element analysis of sample one.\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/div\u003e\n \u003c/caption\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eElements\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eCa\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eSi\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eFe\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eK\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eBa\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eS\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eTi\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eCr\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eZn\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eCu\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003c/tr\u003e\n \u003c/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eContent/%\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e29.75\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e26.45\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e20.10\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e7.44\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e7.30\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e4.22\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e2.70\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e1.56\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e0.36\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e0.12\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cbr\u003e\u003ctable border=\"1\" data-mce-selected=\"1\" style=\"outline: black solid 1px; resize: none; color: rgb(0, 0, 0); font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial;\"\u003e\n \u003ccaption\u003e\n \u003cdiv class=\"CaptionNumber\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif; font-size: 15px;\"\u003eTable 2\u003c/span\u003e\u003c/div\u003e\n \u003cdiv class=\"CaptionContent\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eThe X-ray fluorescence element analysis of sample two.\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/div\u003e\n \u003c/caption\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eElements\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eMn\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eCa\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eCl\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eK\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eSi\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eS\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eTi\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eCu\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003c/tr\u003e\n \u003c/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eContent/%\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e41.94\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e24.19\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e14.21\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e13.56\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e4.20\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e1.42\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e0.36\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e0.12\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cbr\u003e\u003ctable border=\"1\" data-mce-selected=\"1\" style=\"outline: black solid 1px; resize: none; color: rgb(0, 0, 0); font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial;\"\u003e\n \u003ccaption\u003e\n \u003cdiv class=\"CaptionNumber\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif; font-size: 15px;\"\u003eTable 3\u003c/span\u003e\u003c/div\u003e\n \u003cdiv class=\"CaptionContent\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eAmino acid composition ratio (%) in the sample sizing material.\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/div\u003e\n \u003c/caption\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eSample\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eAla\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eGly\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eVal\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eLeu\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eIle\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eSer\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003ePro\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003ePhe\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eAsp\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eGlu\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eHyp\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003c/tr\u003e\n \u003c/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eOne\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e5.8\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e7.3\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e4.6\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e6.4\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e3.7\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e22.0\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e2.4\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e4.2\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e6.6\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e21.1\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e15.8\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eTwo\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e11.3\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e20.5\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e4.7\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e6.9\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e4.1\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e20.2\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e10.1\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e3.1\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e5.5\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e9.1\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\n \u003cp\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e4.6\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n \u003ctfoot\u003e\n \u003ctr\u003e\n \u003ctd colspan=\"12\" style=\"font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 11px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif; font-size: 15px;\"\u003eNote: Asp, Ser, Glu, Pro, Gly, Ala, Val, Ile, Leu, Phe, Hyp in the table are respectively the abbreviations of aspartic acid, serine, glutamic acid, proline, glycine, alanine, valine, isoleucine, leucine, phenylalanine and hydroxyproline.\u003c/span\u003e\u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tfoot\u003e\n\u003c/table\u003e\u003cbr\u003e\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:6.0pt;margin-left:0in;text-align:center;line-height:normal;font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 15px; font-family: Calibri, sans-serif;\"\u003eTable 4\u0026nbsp;\u003c/span\u003e\u003c/strong\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eTensile strength of ramie net before and after 5 days aging.\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n\u003cdiv align=\"center\" style='margin-top:0in;margin-right:0in;margin-bottom:8.0pt;margin-left:0in;text-align:justify;line-height:150%;font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\n \u003ctable style=\"width: 4.3e+2pt;border-collapse:collapse;border:none;\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:120.55pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:18.85pt;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:8.0pt;margin-left:0in;text-align:center;line-height:150%;font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e\u003cspan style=\"line-height: 150%;\"\u003eAging methods\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:70.9pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:18.85pt;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:8.0pt;margin-left:0in;text-align:center;line-height:150%;font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e\u003cspan style=\"line-height: 150%;\"\u003eWithout aging\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:77.95pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:18.85pt;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:8.0pt;margin-left:0in;text-align:center;line-height:150%;font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e\u003cspan style=\"line-height: 150%;\"\u003eDry heat aging\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:77.95pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:18.85pt;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:8.0pt;margin-left:0in;text-align:center;line-height:150%;font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e\u003cspan style=\"line-height: 150%;\"\u003eWet heat aging\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:77.9pt;border-top:solid windowtext 1.5pt;border-left:none;border-bottom:solid windowtext 1.0pt;border-right:none;padding:0in 5.4pt 0in 5.4pt;height:18.85pt;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:8.0pt;margin-left:0in;text-align:center;line-height:150%;font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e\u003cspan style=\"line-height: 150%;\"\u003eUV light aging\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:120.55pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:28.45pt;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:8.0pt;margin-left:0in;text-align:center;line-height:normal;font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eTensile strength /（kN/m）\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:70.9pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:28.45pt;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:8.0pt;margin-left:0in;text-align:center;line-height:normal;font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e1.30\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:77.95pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:28.45pt;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:8.0pt;margin-left:0in;text-align:center;line-height:normal;font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e1.05\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:77.95pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:28.45pt;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:8.0pt;margin-left:0in;text-align:center;line-height:normal;font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e1.08\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:77.9pt;border:none;padding:0in 5.4pt 0in 5.4pt;height:28.45pt;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:8.0pt;margin-left:0in;text-align:center;line-height:normal;font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e1.20\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width:120.55pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:18.85pt;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:8.0pt;margin-left:0in;text-align:center;line-height:normal;font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003eRetention rate/%\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:70.9pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:18.85pt;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:8.0pt;margin-left:0in;text-align:center;line-height:normal;font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e100\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:77.95pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:18.85pt;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:8.0pt;margin-left:0in;text-align:center;line-height:normal;font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e80.67\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:77.95pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:18.85pt;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:8.0pt;margin-left:0in;text-align:center;line-height:normal;font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e82.52\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width:77.9pt;border:none;border-bottom:solid windowtext 1.5pt;padding:0in 5.4pt 0in 5.4pt;height:18.85pt;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:8.0pt;margin-left:0in;text-align:center;line-height:normal;font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u003cspan style=\"font-size: 15px;\"\u003e\u003cspan style=\"font-family: Calibri, sans-serif;\"\u003e91.95\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n \u003c/table\u003e\n\u003c/div\u003e\n\u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;text-align:justify;line-height:150%;font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u003cspan style=\"font-size: 15px; line-height: 150%; font-family: Calibri, sans-serif;\"\u003eNote: The data in the table are the average of 10 times of parallel tests. \u003c/span\u003e\u003c/p\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"npj-heritage-science","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"hsci","sideBox":"Learn more about [Heritage Science](http://heritagesciencejournal.springeropen.com)","snPcode":"40494","submissionUrl":"https://submission.nature.com/new-submission/40494/3","title":"npj Heritage Science","twitterHandle":"@SpringerOpen","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"BMC/SO AJ","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"Painted textiles, ramie fiber, conservation methods, reinforcement, cultural relic.","lastPublishedDoi":"10.21203/rs.3.rs-76692/v2","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-76692/v2","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eTextiles are valuable cultural heritage items that are susceptible to several degradation processes due to their sensitive nature, such as the case of ancient\u003cem\u003e \u003c/em\u003ema colored-paintings. Therefore, it is important to take measures to protect the precious\u003cem\u003e \u003c/em\u003ema artifacts. Generally, ″ma″ includes ramie, hemp, flax, oil flax, kenaf, jute, and so on. In this paper, an examination and analysis of a painted ma textile were the first step in proposing an appropriate conservation treatment. Standard fiber and light microscopy were used to identify the fiber type of the painted ma textile. Moreover, custom-made reinforcement materials and technology were introduced with the principles of compatibility, durability and reversibility. The properties of tensile strength, aging resistance and color alteration of the new material to be added were studied before and after dry heat aging, wet heat aging and UV light aging. After systematic examination and evaluation of the painted ma textile and reinforcement materials, the optimal conservation treatment was established, and exhibition method was established. Our work presents a new method for the conservation of ancient Chinese painted ramie textiles that would promote the protection of these valuable artifacts.\u003c/p\u003e","manuscriptTitle":"A new method for the conservation of ancient colored paintings on ramie textiles","msid":"","msnumber":"","nonDraftVersions":[{"code":2,"date":"2021-01-28 11:08:32","doi":"10.21203/rs.3.rs-76692/v2","editorialEvents":[{"type":"communityComments","content":0},{"type":"editorAssigned","content":"","date":"2021-01-16T00:00:00+00:00","index":"","fulltext":""},{"type":"decision","content":"Accept","date":"2021-01-16T00:00:00+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2021-01-15T23:00:00+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2021-01-15T23:00:00+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"npj-heritage-science","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"hsci","sideBox":"Learn more about [Heritage Science](http://heritagesciencejournal.springeropen.com)","snPcode":"40494","submissionUrl":"https://submission.nature.com/new-submission/40494/3","title":"npj Heritage Science","twitterHandle":"@SpringerOpen","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"BMC/SO AJ","inReviewEnabled":true,"inReviewRevisionsEnabled":true}},{"code":"","date":"2021-01-13 00:00:00","doi":"","editorialEvents":[{"type":"editorAssigned","content":"","date":"2021-01-13T00:00:00+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2021-01-12T23:00:00+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2021-01-12T23:00:00+00:00","index":"","fulltext":""},{"type":"notPreprinted","content":""}],"status":"timeline","journal":{"display":true,"email":"[email protected]","identity":"npj-heritage-science","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"hsci","sideBox":"Learn more about [Heritage Science](http://heritagesciencejournal.springeropen.com)","snPcode":"40494","submissionUrl":"https://submission.nature.com/new-submission/40494/3","title":"npj Heritage Science","twitterHandle":"@SpringerOpen","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"BMC/SO AJ","inReviewEnabled":true,"inReviewRevisionsEnabled":true}},{"code":"","date":"2021-01-11 00:00:00","doi":"","editorialEvents":[{"type":"editorAssigned","content":"","date":"2021-01-11T00:00:00+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2021-01-10T23:00:00+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2021-01-10T23:00:00+00:00","index":"","fulltext":""},{"type":"notPreprinted","content":""}],"status":"timeline","journal":{"display":true,"email":"[email protected]","identity":"npj-heritage-science","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"hsci","sideBox":"Learn more about [Heritage Science](http://heritagesciencejournal.springeropen.com)","snPcode":"40494","submissionUrl":"https://submission.nature.com/new-submission/40494/3","title":"npj Heritage Science","twitterHandle":"@SpringerOpen","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"BMC/SO AJ","inReviewEnabled":true,"inReviewRevisionsEnabled":true}},{"code":"","date":"2021-01-06 00:00:00","doi":"","editorialEvents":[{"type":"decision","content":"Major revision","date":"2021-01-06T00:00:00+00:00","index":"","fulltext":""},{"type":"reviewerAgreed","content":"","date":"2021-01-05T00:00:00+00:00","index":1,"fulltext":""},{"type":"editorInvitedReview","content":"","date":"2021-01-05T00:00:00+00:00","index":1,"fulltext":"Recommendation: Reviewer's comments unavailable due to the journal's policy.\n"},{"type":"editorAssigned","content":"","date":"2021-01-04T00:00:00+00:00","index":"","fulltext":""},{"type":"reviewersInvited","content":"","date":"2021-01-04T00:00:00+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2021-01-03T23:00:00+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2021-01-03T23:00:00+00:00","index":"","fulltext":""},{"type":"notPreprinted","content":""}],"status":"timeline","journal":{"display":true,"email":"[email protected]","identity":"npj-heritage-science","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"hsci","sideBox":"Learn more about [Heritage Science](http://heritagesciencejournal.springeropen.com)","snPcode":"40494","submissionUrl":"https://submission.nature.com/new-submission/40494/3","title":"npj Heritage Science","twitterHandle":"@SpringerOpen","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"BMC/SO AJ","inReviewEnabled":true,"inReviewRevisionsEnabled":true}},{"code":"","date":"2020-12-29 00:00:00","doi":"","editorialEvents":[{"type":"decision","content":"Major revision","date":"2020-12-29T00:00:00+00:00","index":"","fulltext":""},{"type":"reviewerAgreed","content":"","date":"2020-12-22T00:00:00+00:00","index":1,"fulltext":""},{"type":"editorInvitedReview","content":"","date":"2020-12-22T00:00:00+00:00","index":1,"fulltext":"Recommendation: Reviewer's comments unavailable due to the journal's policy.\n"},{"type":"editorAssigned","content":"","date":"2020-12-10T00:00:00+00:00","index":"","fulltext":""},{"type":"reviewersInvited","content":"","date":"2020-12-10T00:00:00+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2020-12-09T23:00:00+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2020-12-09T23:00:00+00:00","index":"","fulltext":""},{"type":"notPreprinted","content":""}],"status":"timeline","journal":{"display":true,"email":"[email protected]","identity":"npj-heritage-science","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"hsci","sideBox":"Learn more about [Heritage Science](http://heritagesciencejournal.springeropen.com)","snPcode":"40494","submissionUrl":"https://submission.nature.com/new-submission/40494/3","title":"npj Heritage Science","twitterHandle":"@SpringerOpen","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"BMC/SO AJ","inReviewEnabled":true,"inReviewRevisionsEnabled":true}},{"code":1,"date":"2020-09-22 18:37:38","doi":"10.21203/rs.3.rs-76692/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Major revision","date":"2020-10-05T12:00:00+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2020-10-03T12:00:00+00:00","index":1,"fulltext":"Recommendation: Reviewer's comments unavailable due to the journal's policy.\n"},{"type":"reviewerAgreed","content":"","date":"2020-09-28T12:00:00+00:00","index":3,"fulltext":""},{"type":"editorInvitedReview","content":"","date":"2020-09-28T12:00:00+00:00","index":3,"fulltext":"Recommendation: Reviewer's comments unavailable due to the journal's policy.\n"},{"type":"editorInvitedReview","content":"","date":"2020-09-28T12:00:00+00:00","index":2,"fulltext":"Recommendation: Reviewer's comments unavailable due to the journal's policy.\n"},{"type":"reviewerAgreed","content":"","date":"2020-09-24T12:00:00+00:00","index":2,"fulltext":""},{"type":"reviewerAgreed","content":"","date":"2020-09-22T12:00:00+00:00","index":1,"fulltext":""},{"type":"reviewersInvited","content":"","date":"2020-09-17T12:00:00+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2020-09-13T12:00:00+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2020-09-12T12:00:00+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2020-09-12T12:00:00+00:00","index":"","fulltext":""},{"type":"submitted","content":"","date":"2020-09-11T12:00:00+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"npj-heritage-science","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"hsci","sideBox":"Learn more about [Heritage Science](http://heritagesciencejournal.springeropen.com)","snPcode":"40494","submissionUrl":"https://submission.nature.com/new-submission/40494/3","title":"npj Heritage Science","twitterHandle":"@SpringerOpen","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"BMC/SO AJ","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"5f261601-39d7-487b-a6b4-a01ff2e5c0f5","owner":[],"postedDate":"January 28th, 2021","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[{"id":2044807,"name":"Cultural Studies"},{"id":2044808,"name":"Art History"}],"tags":[],"updatedAt":"2021-02-07T15:03:40+00:00","versionOfRecord":{"articleIdentity":"rs-76692","link":"https://doi.org/10.1186/s40494-021-00486-4","journal":{"identity":"npj-heritage-science","isVorOnly":false,"title":"npj Heritage Science"},"publishedOn":"2021-01-31 15:03:15","publishedOnDateReadable":"January 31st, 2021"},"versionCreatedAt":"2021-01-28 11:08:32","video":"","vorDoi":"10.1186/s40494-021-00486-4","vorDoiUrl":"https://doi.org/10.1186/s40494-021-00486-4","workflowStages":[]},"version":"v2","identity":"rs-76692","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-76692","identity":"rs-76692","version":["v2"]},"buildId":"FbvkV6FR0MCFSLy54lSbu","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}