Modulation of 17#beta#-estradiol and/or TCDD on the expression of chemokine IL-8 and its receptor CXCR1 by endometrial stromal cells

To explore modulatory effect of the combined effect of estradiol and TCDD on IL-8 expression and auto-endocrine role in endometrial stromal cellsESC, lmmunohistochemistry was used to evaluate the protein translation of CXCR1 in eutopic endometrium and endometriotic tissue of patients with endometriosis. Treatment in vitro of the endometrial stromal cells by estradiol and/or TCDD was to evaluate the modulatory effect of estradiol and TCDD alone or in combination on the CXCR1 expression and IL-8 secretion by endometrial stromal cells. The CXCR1 translation in ESC was determined by flow cytometric assay, and the IL-8 secreting level in supernatant was detected by ELISA. It was found that CXCR1 was more translated by endometriotic tissue than eutopic endometrium. Both estradiol and TCDD inhibited the CXCR1 expression and IL-8 secretion by ESC. The combination of estradiol and TCDD up-regulated the expression of CXCR1 but further inhibited the secretion of IL-8 by the ESC. The combination of estradiol and TCDD show an inhibition effect on IL-8 secretion and auto-endocrine role in ESC. Ectopic ESC exposed to estrogen-related milieu is not account for the increased IL-8 concentration in peritoneal fluid of patients with endometriosis.