Experimental Evaluation of an Antimicrobial Protein from Bacillus Amyloliquefaciens MBL27 for Wound Healing Potential in Rats

Abstract Objective: This study was aimed at assessing the ability of the antimicrobial protein (AMP) produced by Bacillus amyloliquefaciens MBL27 as a potent wound healant. Methods: Rat models were used to study the efficacy of AMP and AMP incorporated chitosan sheet along with control groups.Results: AMP and AMP incorporated chitosan sheet significantly improved wound contraction when compared to controls. Rate of wound contraction (97.23%), decreased period of epithelialization (14 days) and the levels of biochemical markers such as hydroxyproline (collagen), hexosamine, uronic acid and total protein in the granulation tissue on various days of wound healing revealed the wound healing efficacy of the AMP. The histological examinations also correlated well with the biochemical findings, confirming the wound healing efficacy of the AMP. Conclusion: The results indicate the beneficial effects of AMP from B.amyloliquefaciens MBL27 and its potential to be developed into new therapeutic agent for dermal wound healing.