Methylation Analysis in Uygur and Kazak Patients with Hypertension Complicated with Left Ventricular Hypertrophy: Ethnic Difference Based On High-density Genomic DNA Methylation Chips

Background: This study aimed to analyze the methylation level in Uygur and Kazak patientswith hypertension complicated with left ventricular hypertrophy using high-density DNA methylation chips. Methods: Three Uygur and three Kazak patients with hypertension complicated with left ventricular hypertrophy were selected for DNA extraction and bisulfite conversion. Left ventricular hypertrophy was defined by echo Penn convention as left ventricular mass index≥115 g/m2 in men and ≥95 g/m2 in women. Illumina HD450K Infmium Methylation BeadChip was applied to detect 450,000 methylation sites of the whole human genome.The cluster analysis and significance test were carried out to analyze the different methylation levelsin Uygur and Kazak patients with left ventricular hypertrophy. The Delta Data were screened for statistics. Results: A total of 236genes (including 84 hypermethylated and 152 hypomethylated) showed significant differences in the degree of methylation between Uygur and Kazak patients.The average methylation level of significantly different genes was higher in the experimental group compared with the control group.The chromosome distribution of aberrant methylationgenes was quite even. The cluster analysis of the candidate genes showed thataberrant methylation genes in Uygur and Kazak patients with hypertension complicated with left ventricular hypertrophy mainly participated in cell adhesion,signal transduction, and cell cycle. Conclusions: Different methylation genes were found in Uygur and Kazak patients with hypertension complicated with myocardial hypertrophy, providing the basis for further studies on the molecular mechanism and prevention and treatment ofhypertension complicated with target organ damage among different nationalities in Xinjiang.