Comparison of different curative methods on endometriosis in rats

To discuss therapeutic effects of recombinant human interferon-α-2b and leuprorelin on rat endometriosis (EMs) model.

From August to November, 2016, a total of 50 healthy specific pathogen fee(SPF) female Sprague-Dawley (SD) rats were chosen as research subjects. The rat model of abdominal wall EMs was established by autologous uterine transplantation. The successfully established rat EMs model were randomly divided into experimental group A (n=15), experimental group B (n=15) and control group (n=15)according to random number table. There were no significant differences among three groups in the aspects of basic information (P>0.05). The rats in experimental group A received 3 times of 100 000 U of recombinant human interferon-α-2b by subcutaneous injection every 48 h. The experimental group B was daily treated with leuprorelin 20 μg/kg by subcutaneous injection, a total of 28 days. The control group was daily given subcutaneous injection of saline 0.1 mL, a total of 28 days. After the end of 4 weeks treatment, the growth of ectopic lesions in 3 groups were observed, and the expression of vascular endothelial growth factor (VEGF) protein and tumor necrosis factor (TNF)-α protein in ectopic endometrium tissues were detected by immunohistochemical method. All experimental protocols were approved by the Institutional Animal Care and Use Committee.

① Among 50 EMs rats, the successful rate of EMs rat establishment model was 90% (45/50). ② After 4 weeks treatment, there was significant difference in ectopic lesions volume among three groups (F=139.332, P<0.001). Among them, the ectopic lesions volume of rats in experimental group A and experimental group B were both smaller than that of rats in control goup (LSD-t=13.460, 15.280; P<0.001); the ectopic lesions volume of rats in experimental group B was smaller than that of rats in experimental group A, which had no significant (LSD-t=1.820, P=0.076). Besides, there were significant differences in ectopic volume of rats before and after the treatment in experimental group A and B (t=9.413, 14.545; P<0.001). ③ After 4 weeks treatment, there were no significant in the expression of VEGF protein and TNF-α protein among 3 groups (F=224.261, 225.391; P<0.001). The expression of VEGF protein and TNF-α protein of experimental group A and B were significant lower than those of control group (experimental group A vs control group: LSD-t=17.258, 19.121; P<0.001; experimental group B vs control group: LSD-t=19.260, 17.552; P<0.001).

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