Stabilization of Coarse‑Grained Saline Soils Using Salt‑Tolerant Domesticated Microorganism via Microbially Induced Calcium Carbonate Precipitation

Stabilization of Coarse‑Grained Saline Soils Using Salt‑Tolerant Domesticated Microorganism via Microbially Induced Calcium Carbonate Precipitation | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 4 August 2025 V1 Latest version Share on Stabilization of Coarse‑Grained Saline Soils Using Salt‑Tolerant Domesticated Microorganism via Microbially Induced Calcium Carbonate Precipitation Authors : Tianle Liu , Hourun Lai , Shaojun Zheng [email protected] , Chengxiang Tang , Shunbo Qin , Huaimeng Gu , Mingsheng Chen , and Guosheng Jiang Authors Info & Affiliations https://doi.org/10.22541/au.175431645.55751045/v1 183 views 129 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Microbially induced calcium carbonate precipitation (MICP) has been applied in saline soil management due to its environmental sustainability. However, complex high-salinity conditions can affect microbial metabolism and hinder the precipitation of calcium carbonate. These conditions thus weaken the management performance of MICP. In this study, Sporosarcina pasteurii ( S. pasteurii ) was domesticated by common salts found in saline soils (chlorides, sulfates, and carbonates) to improve the applicability of MICP for stabilizing coarse-grained saline soils. The mechanical properties, pore structure, and microstructure of the treated coarse-grained saline soils (chloride and sulfate) were characterized to evaluate the stabilization effect of MICP. The results show that the growth-limiting factors of S. pasteurii depend on the type of salt present. In chloride and sulfate environments, ion concentration is the main limiting factor. In carbonate environments, the increase in pH is the principal inhibitory factor. The salt-domesticated S. pasteurii (HSC) exhibited enhanced adaptation to saline conditions. After 14 treatment cycles, the unconfined compressive strengths of the chloride and sulfate saline soils reached 1.63 MPa and 0.92 MPa, respectively. MICP generates calcium carbonate cementitious within the voids between soil particles, thereby stabilizing the saline soils. The improvement in pore structure is especially pronounced for the chloride saline soil. Furthermore, the calcium carbonate produced by HSC in high-salinity coarse-grained saline soils not only provides binding and bridging functions but also forms an overlaying function that further enhances stability. Therefore, MICP is also suitable for high-salinity environments and can aid in the management of complex saline soils. Supplementary Material File (manuscript.docx) Download 49.79 MB Information & Authors Information Version history V1 Version 1 04 August 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords micp saline soils salt-tolerant domestication soil reinforcement urease activity Authors Affiliations Tianle Liu China University of Geosciences View all articles by this author Hourun Lai China University of Geosciences View all articles by this author Shaojun Zheng [email protected] China University of Geosciences View all articles by this author Chengxiang Tang China University of Geosciences View all articles by this author Shunbo Qin China University of Geosciences View all articles by this author Huaimeng Gu China University of Geosciences View all articles by this author Mingsheng Chen China University of Geosciences View all articles by this author Guosheng Jiang China University of Geosciences View all articles by this author Metrics & Citations Metrics Article Usage 183 views 129 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Tianle Liu, Hourun Lai, Shaojun Zheng, et al. Stabilization of Coarse‑Grained Saline Soils Using Salt‑Tolerant Domesticated Microorganism via Microbially Induced Calcium Carbonate Precipitation. Authorea . 04 August 2025. DOI: https://doi.org/10.22541/au.175431645.55751045/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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