The Role of Protein Kinase Activators and ZFN in Reducing the Harmful Effects of Total Body Irradiation

Objective: AIDS patients are predisposed to develop certain tumors. Radiation therapy is one of the main treatments for cancer. However, exposure to ionizing radiation leads to defects in the lymphatic system. The cells with the highest sensitivity to radiation are the T lymphocytes. We aim to investigate the effect of irradiation on the ratio of CD4 to CD8, and to oppose this effect by using Bryostatin (B) and ZFN and ART (Anti-Retroviral Therapy). So Balb/C mice aged 6-8 weeks were whole-body irradiated with 1.5 Gy of γ-rays, was then mice were treated with ZFN, Bryostatin intraperitoneally. And orally Antiviral treatment. At the end of the study, CD4/CD8 was measured by flow cytometry. Results: Irradiation caused significant pathological changes that led to a clear reversal of CD4/CD8 ratio significantly within the normal radiating group. there are no significant differences in CD4/CD8 ratio between all groups treated with ZFN+ X, Z+ B+ X, ART+ X and normal group.So our study suggests the effect of Bryostatin in protecting living organs that were exposed to irradiation in mice. ZFN cuts off both BAX and BAK, and thus decreases apoptotic proteins, reducing cell death.