Research on miRNAs of exosomes’ role on porcine oocyte development by extracted from follicular fluid

Follicular development is crucial to oocyte maturation in these special surrounds, and the follicular size closely related to oocyte maturation. In order to clarify porcine oocyte maturation molecular mechanism, obtained exosomes miRNAs from porcine follicular fluid (PFF) are sequenced and researched among different follicular size as described in the methods. The results firstly show that PFF exosomes can be successfully isolated, and almost all valid reads of sequencing data of PFF exosomes can be successfully mapped to the porcine genome database. Using hierarchical clustering methods, it secondly finds that significantly expressed miRNAs can also be clustered in A, B C and D groups in heatmap according to different size follicles, which possibly to target mRNAs genes related to porcine oocyte development. Thirdly, through choosing ten significantly expressed miRNAs to predict the targeting genes for further GO analysis, the results show that the expression of neurotransmitter secretion genes changes greatly, and many targeting genes involves in regulation of FSH secretion, which is very closely related to oocyte maturation in growing follicles. Further, taking Pathway analysis for these targeting genes this ten choosing miRNAs, the results show that the pathways mainly related to the biosynthesis pathway of TGF-beta signaling pathway, which is very closely related to reproductive system function. Finally, these miRNAs in PFF EXs may provide a valuable addition about the mechanism of porcine oocyte maturation, which could be chosen as some molecular biomarkers to choose the high-quality oocytes for further porcine embryo production in vitro or transgenic animals research in the future.