Establishment of Nude Mouse Endometriosis Model and Expression of Steroid Hormone Receptors

Objective To establish an experimental endometriosis model using nude mouse as a xenographic host for clinical study of endometriosis. MethodExperimental groups were implanted with late secretory phase endometrial tissues of normal and endometriosis patients. Nude mice of experimental group Ⅰ were ovariectomied and experimental group Ⅱ were not. Nude mice of control group were implanted with greater omentum. All groups were given estradiol support. Nude mice were killed at 5?d, 10?d, 15?d, 20?d, 25? d and 30?d after transplantation to observe the growth of ectopic lesions. Meanwhile, we detected the expression of steroid hormone receptors by immunohistochemistry method. ResultsPeritoneal implantation of endometrial fragments was observed from 5 days, which revealed endometrial gland and stroma in ectopic lesions. And the adhension was more and more severe with the growth time. There were differences of ectopic lesions neither between the implantation by normal endometrium and endometrium from patients with endometriosis, nor between experimental group Ⅰ and experimental group Ⅱ. Immunochemistry revealed also no differences and almost weak expression of steroid hormone receptors. ConclusionThe nude mouse is an appropriate model for the study of the early phase of endometriosis.