The Diamesinae (Diptera: Chironomidae) in high-altitude Andean streams using morphological and molecular approaches.

The Diamesinae (Diptera: Chironomidae) in high-altitude Andean streams using morphological and molecular approaches. | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL Ecology and Evolution This is a preprint and has not been peer reviewed. Data may be preliminary. 6 March 2025 V1 Latest version Share on The Diamesinae (Diptera: Chironomidae) in high-altitude Andean streams using morphological and molecular approaches. Authors : Diana Hoyos Jaramillo 0000-0002-8042-9661 [email protected] , Raul Acosta , Carles Ribera , Núria Bonada , and Narcís Prat Authors Info & Affiliations https://doi.org/10.22541/au.174127010.01135262/v1 Published Ecology and Evolution Version of record Peer review timeline 325 views 222 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Diamesinae is one of the eleven subfamilies of Chironomidae, characterized by its preference for cold waters, typically found in mountainous regions. We aimed to study the biodiversity and distribution of two genera of this subfamily found in high-altitude streams in the Andes (Paraheptagyia and Limaya), by combining morphological and molecular analyses. A database with 190 larval specimens of Diamesinae, collected between July and October of 2011 from 20 streams in Colombia, Ecuador, and Peru, at altitudes above 2000 m.a.s.l. was used. Paraheptagyia was found in the three countries, whereas Limaya was only found in Ecuador and Perú. Morphological analyses were based on 105 larvae mountings, using several specimens for each morphospecies, as a result, the antennal ratio (AR) was a valuable differentiator for Limaya, whereas Paraheptagyia could not be distinguished by using morphological characters. The ABGD, ASAP, and bPTP methods were used to delineate operational taxonomic units (OTUs) using 130 Molecular sequences (113 for Paraheptagyia and 17 for Limaya). For the genus Limaya, two OTUs were identified using all methods, one for Ecuador and one for Peru. Seven OTUs were identified for the genus Paraheptagyia. Five of the seven OTUs were found in Peru and the other two were shared between Ecuador and Colombia. The presence of the Huancabamba depression on the border between Ecuador and Peru likely acts as a genetic barrier, limiting gene flow. The difference in branch length between Peruvian and Ecuadorian/Colombian species supports Brundin’s (1966) hypothesis, indicating a south-to-north colonization pattern. Supplementary Material File (diamesinae high altitude andes.docx) Download 92.28 KB File (figartd.pdf) Download 1.03 MB File (tables dchj.xlsx) Download 255.95 KB Information & Authors Information Version history V1 Version 1 06 March 2025 Peer review timeline Published Ecology and Evolution Version of Record 27 Nov 2025 Published Copyright This work is licensed under a Non Exclusive No Reuse License. Collection Ecology and Evolution Keywords freshwater invertebrate molecular evolution molecular genetics natural history Authors Affiliations Diana Hoyos Jaramillo 0000-0002-8042-9661 [email protected] Universitat de Barcelona Facultat de Biologia View all articles by this author Raul Acosta IDAEA View all articles by this author Carles Ribera Universitat de Barcelona View all articles by this author Núria Bonada Universitat de Barcelona Facultat de Biologia View all articles by this author Narcís Prat Universitat de Barcelona Facultat de Biologia View all articles by this author Metrics & Citations Metrics Article Usage 325 views 222 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Diana Hoyos Jaramillo, Raul Acosta, Carles Ribera, et al. The Diamesinae (Diptera: Chironomidae) in high-altitude Andean streams using morphological and molecular approaches.. Authorea . 06 March 2025. DOI: https://doi.org/10.22541/au.174127010.01135262/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . Format Please select one from the list RIS (ProCite, Reference Manager) EndNote BibTex Medlars RefWorks Direct import Tips for downloading citations document.getElementById('citMgrHelpLink').addEventListener('click', function() { popupHelp(this.href); return false; }); $(".js__slcInclude").on("change", function(e){ if ($(this).val() == 'refworks') $('#direct').prop("checked", false); $('#direct').prop("disabled", ($(this).val() == 'refworks')); }); View Options View options PDF View PDF Figures Tables Media Share Share Share article link Copy Link Copied! Copying failed. 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