Screening and verification of specific antibodies against Cysticercus cellulosae by antibody microarray technology

Abstract Cysticercosis occurs primarily in economically underdeveloped regions of the world, limiting diagnosis by conventional imaging and serologic tests. In addition, due to the limitations of traditional diagnostic methods, there has been an increased demand for cysticercosis markers with high specificity and sensitivity.In this study, antibody microarray technology was used for high-throughput screening of Cysticercus cellulosae, patient serum, and healthy human serum. Differential expression analysis and cluster analysis identified 271 antibodies with significantly different expression levels. The six antibodies were found to be significantly different in healthy serum compared to patient serum and parasite samples, indicating their potential as markers for the diagnosis of cysticercosis. It will be important for the diagnosis and treatment of this foodborne disease to further develop and utilize these markers.