Network Connectivity and Local Hydrology Drive DOM Composition in a Non-Perennial Stream

Network Connectivity and Local Hydrology Drive DOM Composition in a Non-Perennial Stream | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 9 January 2026 V1 Latest version Share on Network Connectivity and Local Hydrology Drive DOM Composition in a Non-Perennial Stream Authors : Sarah Megan Flynn 0000-0002-1758-6947 , Rebecca L Hale , Erin Cedar Seybold 0000-0002-0365-2333 , Stephen Plont , Michelle Hope Busch 0000-0003-4536-3000 , Alexi Sommerville 0009-0003-3926-8880 , Connor L Brown 0000-0002-9680-8930 , and Amy J Burgin 0000-0001-8489-4002 [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.176800018.86081859/v1 124 views 83 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Nonperennial streams are dynamic sites of biogeochemical processing, yet much remains to be learned about how hydrologic connectivity impacts the composition of dissolved organic matter (DOM) at the network and local scale. We characterized spatial and temporal patterns of DOM composition in a non-perennial prairie stream network using three years of hydrologic data and surface water fluorescence spectroscopy through repeated sampling. Principal component analyses were used to reduce dimensionality and uncover major controls of DOM composition. Humic, terrestrial components were found to explain the most variance in the model. Humic, terrestrial components of DOM were controlled by network conditions, and were highest during dry down phases. Microbial components of DOM were associated with local flow conditions and water persistence. DOM was more microbially derived and autochthonous at sites that remained persistently wet and flowing. The interaction between network and local scales and their impact on DOM composition is phase dependent, such that local microbial activity can be reduced during high connectivity (due to transport) but enhanced during low or transitional flows (due to retention). These findings suggest that hydrologic fragmentation in nonperennial systems modulates not only DOM source and transformation but also its downstream bioavailability, with implications for carbon cycling under shifting climate and flow regimes. Supplementary Material File (1055369_0_merged_1765212108.pdf) Download 2.97 MB File (flynn_agu_manuscript_dom_resubmitted_2025jg009568.docx) Download 81.84 KB File (flynn_agu_supporting_information_2025jg009568.docx) Download 1008.42 KB Information & Authors Information Version history V1 Version 1 09 January 2026 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords biogeochemistry dissolved organic matter hydrology non-perennial streams Authors Affiliations Sarah Megan Flynn 0000-0002-1758-6947 University of Kansas View all articles by this author Rebecca L Hale Smithsonian Instution View all articles by this author Erin Cedar Seybold 0000-0002-0365-2333 Kansas Geological Survey, University of Kansas View all articles by this author Stephen Plont University of Georgia View all articles by this author Michelle Hope Busch 0000-0003-4536-3000 Michigan State University View all articles by this author Alexi Sommerville 0009-0003-3926-8880 Kansas Geological Survey; Department of Geology View all articles by this author Connor L Brown 0000-0002-9680-8930 The University of Kansas; Kansas Geological Survey View all articles by this author Amy J Burgin 0000-0001-8489-4002 [email protected] Iowa State University View all articles by this author Funding Information National Science Foundation 2019603 Amy Burgin Metrics & Citations Metrics Article Usage 124 views 83 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Sarah Megan Flynn, Rebecca L Hale, Erin Cedar Seybold, et al. Network Connectivity and Local Hydrology Drive DOM Composition in a Non-Perennial Stream. Authorea . 09 January 2026. DOI: https://doi.org/10.22541/au.176800018.86081859/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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