Time Course of Renal Ischemia/Reperfusion and Distance Organ; Lung Dysfunction in Male and Female Rats

Abstract Background Renal ischemia/reperfusion injury (IRI) may influence distance organ such as lung. The severity of IRI induced - lung injury can be affected by gender. The aim of this study was to determine the role of gender in IRI induced- lung injury in different of renal reperfusion time. Methods 80 male and female Wistar rats were assigned into 8 groups; 4 groups in each gender including sham, renal ischemia (ISC) for 45 min by clamping renal vessels followed by 3 hr, 24 hr or 48 hr reperfusion.blood samples were obtained for measuring the serum level of blood urea nitrogen (BUN), creatinine (Cr), nitrite, and malondialdehyde (MDA). The kidneys and lung tissues were removed and used for MDA and nitrite measurements and the histological changes evaluation.the percentage of lung water content was calculated. Results In both genders, the rise in Cr and BUN reached the peak at 24 h reperfusion. In 3 h reperfusion female rats, lead to significant increase in serum level of nitrite compared with males. In male rats subjected to 3 h reperfusion, the renal MDA level increased but not seen in females. The enhanced lung tissues damages were depended to reperfusion time in both genders. The water content of lung was reduced in 3 h of reperfusion groups. Conclusions IRI caused kidney and lung dysfunction depends on reperfusion time. Considering gender difference, female gender may be more sensitive to alteration of nitrite level compared with males. It seems that the effect of IRI is more rapid in males than females.