High thrombospondin 1 expression is associated with aging and poor prognosis in patients with acute myeloid leukemia

AbstractAcute myeloid leukemia is a heterogeneous disease with high morbidity and mortality under current treatment strategies. We screened the key gene thrombospondin 1 by analyzing The Cancer Genome Atlas and Gene Expression Omnibus databases using a bioinformatics approach and further analyzed it using clinical data from The Cancer Genome Atlas database. For validation, we collected bone marrow samples from 20 patients with acute myeloid leukemia and 10 healthy controls. Quantitative polymerase chain reaction and enzyme-linked immunosorbent assay were conducted to quantify the platelet-reactive thrombospondin 1 mRNA and protein expression. Then, we analyzed the different levels in patients and controls using clinical data. We discovered that thrombospondin 1 expression is upregulated in patients with acute myeloid leukemia, and high thrombospondin 1 expression is associated with age and poor prognosis. Thus, as an age-related factor, thrombospondin 1 may be a potential factor in the prognostic prediction of patients with acute myeloid leukemia.