Bioactivity and GC-MS Profile of Extract from Static Cultures of Fungi Isolated from the Leaf of Solanum lycopersicum

Bioactivity and GC-MS Profile of Extract from Static Cultures of Fungi Isolated from the Leaf of Solanum lycopersicum | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Bioactivity and GC-MS Profile of Extract from Static Cultures of Fungi Isolated from the Leaf of Solanum lycopersicum Israel Tefe, Emmanuel Kyame Oppong, Mahama Alhassan, Christopher Yao Gadasu, and 4 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6428166/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract This research investigated bioactive compounds produced by endophytic fungi isolated from Solanum lycopersicum (tomato) leaves, with a focus on antimicrobial and antioxidant properties. The fungal extract exhibited significant antimicrobial activity against Staphylococcus aureus , Streptococcus pyogenes , Enterococcus faecalis , Klebsiella pneumoniae and Candida albicans . The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values were 0.8 mg/mL, demonstrating the extract's potency, particularly against E. faecalis . The extract also showed antioxidant activity, with a 34.9% inhibition rate. TLC analysis, using a hexane: ethyl acetate: acetic acid (25:25:3) solvent system, identified two distinct spots (spots A and B) with Rf values of 0.50 and 0.80, respectively. Visualisation with p -Anisaldehyde revealed pink and deep blue colours, confirming the presence of various bioactive compounds. This research highlights the potential of endophytic fungi as valuable sources of antimicrobial and antioxidant agents, with significant implications for pharmaceuticals and public health. Natural Product Chemistry Rotary Evaporator Solanum lycopersicum GC-MS Agar well diffusion Thin-layer chromatography Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Highlights Fungi living inside tomato leaves were grown in static cultures to explore their potential. GC-MS analysis uncovered several useful compounds, including Norgestrel and Tyrosol acetate. The fungal extract successfully fought off harmful bacteria and yeast, showing strong antimicrobial effects. It was especially effective against E. faecalis , with a low MIC/MBC of 0.8 mg/mL. The extract also showed antioxidant activity (34.9%), and TLC revealed a mix of bioactive plant-like compounds. 1.0 Introduction The role natural products play in modern biotechnology cannot be overlooked as they contribute to over half of all currently available drugs( 1 ). Approximately 85% of traditional medicine practices rely on plant extracts, and about 80% of individuals in developing countries depend on these remedies for their primary healthcare needs( 2 ). Additionally, natural sources have played a significant role in cancer treatment, with 62% of the 87% of anticancer drugs approved in the past decade being derived from natural compounds. Notable examples include paclitaxel ( I ), from Taxus brevifolia , and vinblastine, obtained from Catharanthus roseus ( 3 ). Organic compounds isolated from organisms have proved to be a rich reservoir of novel bioactive molecules with pharmacological potential( 4 ). This continuous exploration of natural compounds has led to the discovery of impactful medications such as penicillin ( II) , vincristine, and artemisinin, all of which have made substantial contributions to human health and well-being( 5 ). Natural compounds combat bacterial resistance mechanisms, such as biofilm formation, which can be disrupted by certain flavonoids. Some of these natural compounds possess distinct structures and mechanisms of action that set them apart from traditional antibiotics. For instance, magainin, antimicrobial peptides found in amphibian skin, can disrupt bacterial cell membranes by forming pores, ultimately leading to cell lysis ( 6 ). In addition to their unique properties, natural compounds offer a cost-effective alternative to synthetic drugs. By utilizing abundant resources in nature, such as plants, fungi, and microorganisms, researchers can harness therapeutic compounds without relying heavily on expensive synthetic processes. This natural abundance reduces production costs, making drug development more economical compared to synthetic alternatives, which often require complex synthesis and lengthy validation phases ( 7 )( 8 ). Natural products have proven to be a valuable source of novel compounds with distinct structures and bioactivities that are not commonly found in synthetic drugs. For example, paclitaxel, a potent chemotherapy agent derived from the Pacific yew plant, has been widely employed in cancer treatment( 9 ). Similarly, penicillin, discovered from the Penicillium notatum mold, revolutionized antibiotic therapy and transformed modern medicine. These examples highlight how nature continues to inspire innovative pharmacological breakthroughs and expand opportunities for drug discovery( 10 ). This research explores the bioactivity potential derived from tomato plant leaves, with a particular focus on their antimicrobial and antioxidant properties ( 11 ). The bioactive compounds present in the fungal extracts were extracted using ethyl acetate and then subjected to TLC and GC-MS analysis ( 12 ). Additionally, the research determined the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values of the extract against five microbial strains. 2.0 Methodology The study collected samples from Tsito Sec. Tech. An area in the Volta Region, Ghana, used a procedure involving a 3% malt extract and 6% glucose-enriched media broth to create a suitable environment for fungi proliferation. After three weeks of sub-culturing, a specific colony was carefully extracted using sterilized forceps and an inoculation loop and transferred into a sterile 2 dm 3 flat-bottomed flask containing a medium consisting of 3% malt extract and 6% glucose ( 13 ). The fungal cultures were harvested by filtering the mycelia from the broth using a muslin sieve after eight weeks. The extract was extracted using ethyl acetate, after which the organic layer was subjected to concentration using a rotary evaporator( 14 ). A solvent system was prepared in a 25:25:3 ratio and poured into a TLC chamber and a small portion of the TLTS extract was dissolved in ethyl acetate and spotted on a TLC plate to dry. A p -anisaldehyde spray reagent was prepared and sprayed on the plates, heated at 105°C for 15 minutes and visualized. The TLC plates was observed to have pink and deep blue colours at different distances from the baseline ( 15 ). The GC-MS analysis was conducted using a Thermo Scientific GC TRACE 1300 gas chromatograph paired with an ISQ QD Mass Spectrometer. Its data interpreted using the National Institute of Standards and Technology (NIST) database. The fungal extract tested against Enterococcus faecalis , Staphylococcus aureus , Klebsiella pneumoniae, Streptococcus pyogenes and Candida albicans for their antifungal and antibacterial effects. Antimicrobial activity was assessed using the agar well diffusion method, following established standardized protocols ( 16 ). The MIC of the fungal extract was determined using the Triphenyl Tetrazolium Chloride (TTC)-MIC assay. In this assay, a pink colour indicated bacterial growth, while no colour changes signified successful inhibition of bacterial growth ( 17 ). The DPPH (1,1-diphenyl-2-picrylhydrazyl) assay was employed to assess the antioxidant activity of the TLTS extract using the protocol of (Drawell, DNM-9602) ( 18 ). 3.0 Results and Discussions 3.1 Culturing and TLC Analysis Solanum lycopersicum leaves were pre-treated with 70% alcohol and sub-cultured for three weeks. After two weeks, TLTS1 showed white mycelia growth, while other sub-cultures had black with white patches. White mycelia growth is useful in fungal culture studies, indicating fungal species and potential biocontrol agents( 19 ). A solvent system of Hexane: Ethyl acetate: Acetic acid (25:25:3) was used as a mobile phase, which revealed 2 spots with Rf values of 0.50 (spot A) and 0.8 (spot B) as shown in the figure below. The TLC chromatogram showed two distinct colours (pink and deep blue) with p -Anisaldehyde visualizing reagent. The presence of these colours (pink and blue) showed the presence of alkaloids and phenolic compounds, respectively ( 20 ). 3.2 GC-MS Analysis A total of 18 most probable compounds were identified in the TLTS extract using GC-MS. This indicates that TLTS extract contains various groups of compounds. Table 1 includes the name, retention time, and the mass-to-charge ratio of the compounds. Table 1 Compounds that are found in TLTS extract by GC-MS S/N Name Of Compound R. T M. F M/Z 1 Hexanoic acid, 2-phenylethyl ester 6.00 C 14 H 20 O 2 220 2 Benzeneethanol, 4-hydroxy- 8.43 C 8 H 10 O 2 138 3 2,4-Hexadienedioic acid 9.18 C 6 H 6 O 4 142 4 2-Furancarboxylic acid, 5-(hydroxymethyl)- 9.36 C 6 H 6 O 4 142 5 Benzoic acid, 3-hydroxy- 9.91 C 7 H 6 O 3 138 6 Tyrosol, acetate 10. 04 C 10 H 12 O 3 180 7 Bisabolol oxide B 14. 42 C 15 H 26 O 2 238 8 Hexadecanoic acid, ethyl ester 15. 14 C 18 H 36 O 2 284 9 Linoelaidic acid 17. 26 C 18 H 32 O 2 280 10 Octadecanoic acid 17. 64 C 18 H 36 O 2 284 11 N,N'-Bis(Carbobenzylox y)-lysine methyl(ester) 19. 48 C 23 H 28 N 2 O 6 428 12 Spiculesporic acid 21. 54 C 17 H 28 O 6 328 13 (9Z,12Z)-Phenethyl octadeca-9,12-dienoate 27. 74 C 26 H 40 O 2 384 14 Solasonine 8.02 C 45 H 73 NO 16 883 15 Tridemorph 11. 14 C 19 H 39 NO 297 16 Norgestrel 17. 54 C 21 H 28 O 2 312 17 Doconexent 17. 54 C 22 H 32 O 2 328 18 Santamarine 17. 95 C 15 H 20 O 3 248 Table 2 Antimicrobial activity of compounds found in TLTS extract S/N Name of Compound Antimicrobial activity Reference 1 Hexanoic acid, 2-phenylethyl ester Antibacterial ( 21 ) 2 Benzeneethanol, 4-hydroxy- Antioxidant ( 22 ) 3 2,4-Hexadienedioic acid Antimicrobial ( 23 ) 4 2-Furancarboxylic acid, 5-(hydroxymethyl)- antioxidant ( 24 ) 5 Benzoic acid, 3-hydroxy- Antioxidant ( 25 ) 6 Tyrosol, acetate Antioxidant ( 26 ) 7 Bisabolol oxide B Antimicrobial ( 27 ) 8 Hexadecanoic acid, ethyl ester Antimicrobial ( 28 ) 9 Linoelaidic acid Antimicrobial ( 29 ) 10 Octadecanoic acid Antimicrobial ( 30 ) 11 N, N'-Bis (Carbobenzylox y)-lysine methyl(ester) Antimicrobial ( 31 ) 12 Spiculesporic acid Antimicrobial, antifungal ( 32 ) 13 (9Z,12Z)-Phenethyl octadeca-9,12-dienoate Antimicrobial ( 33 ) 14 Solasonine Antimicrobial ( 34 ) 15 Tridemorph Antifungal ( 35 ) 16 Norgestrel Progestogenic, anti-estrogenic ( 36 ) 17 Doconexent Antioxidant ( 37 ) 18 Santamarine Anticancer ( 38 ) The eighteen ( 18 ) different compounds identified through GC-MS analysis of the extract of TLTS (Table 1 ) indicate a rich chemical reservoir of the TLTS extract. Some of the components in TLTS extract have been found to exhibit antimicrobial, antioxidant, antifungal, and anticancer properties, as documented in the literature (Table 2 ). Fatty acid derivatives such as Hexadecanoic acid ( 39 ) and Octadecanoic acid have demonstrated antimicrobial properties through their membrane disruption abilities, which is shown in the zone of inhibition against the bacterial strains ( 30 ), (Table 3 ). 3.3 Rationalization of GC-MS Fragmented Compounds 3.3.1 Tridemorph Tridemorph exhibited six ( 6 ) significant peaks at m/z 297, 128, 71, 57, 43, and 29 within the electron impact (EI) mass spectrum. Among these fragment ions, the parent ion peak at m/z 297 was identified. This peak emerged as a result of the ejection of an electron from the molecular ion. The parent ion was detected on the spectrum, signifying the molecular weight of the compound. The pentyl cation at m/z = 71 with 5% abundance appeared on the spectrum due to homolytic cleavage of the C-C single bond through route (a) . This fragment ion further undergoes fragmentation to lose methyl radical as a result of cleavage of the C-C single bond to generate butyl radical cation at m/z = 57 with 20% abundance via pathway (b) . The butyl radical cation eliminated methyl radical through homolytic cleavage at pathway (c) to produce propyl cation at m/z = 43 with an abundance of 48% on the spectrum. Again, the propyl cation also fragments homolytically through route (d) by liberation of methyl radical to generate ethyl radical cation at m/z = 29 with 18% abundance. The base peak at m/z = 128 with 100% abundance resulted from the homolytic cleavage of the C-N bond linking the morpholine ring to the aliphatic chain, leading to the formation of the morpholinium radical cation via pathway (e) . Consequently, the ions identified in the analysis correspond to the expected fragmentation pattern of tridemorph, thereby providing enough evidence for the compound’s identification. 3.3.2 Tyrosol, acetate Tyrosol acetate exhibited five ( 5 ) prominent peaks at m/z 107, 120, 121, and 180 within the electron impact (EI) mass spectrum. Among these fragment ions, the precursor parent ion peak at m/z 180 was identified. This peak arose as a result of an electron being dislodged from the molecular structure. The parent ion was observed at highest mass that occur at an even value of mass-to charge in the spectrum, confirming the molecular weight of the compound. The loss of the (C 3 H 5 O 2 ) radical through pathway (a) via homolytic cleavage of the ethylene bridge connecting the phenol and the ether moiety, produced p-cresol radical cation at m/z 107 which was prominently displayed at the midpoint of the spectrum with abundance, 45%. Pathway (b) involved an alpha cleavage of the ether bond, corresponding to the fragment ion observed at m/z 121 in the spectrum with 15% abundance. The base peak at m/z 120 with 100% abundance was displayed in the spectrum due to loss of hydrogen radical from (C 2 H 3 O 2 ) ion that resulted in carbocation rearrangement to produce a more stable ion as illustrated in pathway (c) . This fragment ion serves as a distinguishing feature of Tyrosol acetate, frequently employed as a diagnostic fragment for the identification of the compound. Consequently, the ions observed in the GC-MS analysis align with the anticipated fragmentation pattern of Tyrosol acetate, thereby providing compelling evidence for the identification of the compound as Tyrosol acetate. 3.4 Antimicrobial and antioxidant activity of TLTS extract Five microbial strains, E. faecalis , S. pyogenes , K. pneumoniae , S. aureus , and C. albicans , were selected based on their resistance to antimicrobial agents and disease characteristics. These microbial strains are involved in various clinical diseases such as skin infections, pharyngitis, urinary tract infections, and blood infections ( 40 ). 3.4.1 Zone of Inhibition The TLTS extract showed strong antimicrobial properties against E. faecalis , a leading cause of infections in hospitals due to its resistance to some antibiotics. However, the TLTS extract’s efficiency varies significantly with the type of pathogen, as different bacteria have distinct cell wall compositions and structures (41). Gram-positive bacteria such as S. aureus have a robust peptidoglycan layer, making them more vulnerable to certain chemical compounds in the TLTS extract. Gram-negative bacteria such as K. pneumoniae have an outer membrane that can serve as a barrier to antibacterial compounds. The suppressive impact on C. albicans by the TLTS extract suggests the potential of the extract as a broad-spectrum treatment, especially in immunocompromised patients with severe Candida infections ( 42 ). 3.5 Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC ) The TLTS extract effectively combats pathogens like E. faecalis , S. pyogenes , and K. pneumoniae , with MIC and MBC values of 0.8, making it suitable for clinical use due to lower potential side effects. However, S. aureus and C. albicans show more resistance, necessitating further study( 43 ). On the other hand, reported MIC and MBC values of 8.0 ± 0.18 and 12.0 ± 0.17 mg/mL, respectively, against the same microbes. These variations are likely due to differences in extraction and testing methods. 3.6 The Free Radical Scavenging Activity of TLTS Extracts This research also assessed the antioxidant activity of TLTS extract using the DPPH assay, which revealed varying levels of activity with a maximum inhibition percentage of 34.9%. The lowest absorbance (0.829) indicates the most substantial inhibition, suggesting that the extract effectively reduces DPPH radicals. In contrast, the highest absorbance (0.989) corresponds to a lower inhibition of 22.3%, pointing to different antioxidant concentrations within the extract. This variability suggests that not all components share the same antioxidant properties. The extract's 34.9% inhibition indicates its potential as a functional food. Additionally, compounds such as 2,4-Hexadienedioic acid and Bisabolol oxide B are noted for their antimicrobial and antifungal effects, which support the development of multi-target therapies ( 44 ). Table 3 Microbial activity and Antioxidant effectiveness of TLTS Extract Zone of Inhibition MIC MBC Antioxidant Activity Organism TLTS S/N Abso. Extract %Inhibition E. faecalis (NCTC 13379) 13 0.8 0.8 1 0.829 34.9 S.pyogenes (NCTC 12696) 7 0.8 0.8 2 0.929 27.0 K.pneumonae (NCTC 13348) 6 0.8 0.8 3 0.989 22.3 S. aureus (NCTC 12973) 9 1 1 4 0.924 27.4 C. albicans (ATCC90028) 10 1 1 5 0953 25.1 4.0 Conclusion This research extracted bioactive compounds from endophytic fungi of Solanum lycopersicum leaves and identified them using GC-MS. A diverse range of secondary metabolites, including Norgestrel, Tyrosol, Bisabolol oxide B, Doconexent, and Santamarine, were among the major compounds produced. The TLTS extract exhibited significant antimicrobial effects, particularly against Enterococcus faecalis . These results not only emphasise the potential of endophytic fungi as a promising source of therapeutic agents, especially in the context of rising antibiotic resistance, but also open avenues for their applications in pharmaceuticals. Future research should focus on elucidating these compounds' mechanisms of action, evaluating their efficacy in vivo, and considering the ecological importance of endophytic fungi in promoting plant health and sustainability. Declarations Acknowledgements I'm truly grateful to Prof. Emmanuel Kyame Oppong for his guidance and support. Thanks also to the Department of Chemistry, University of Education, Winneba, for creating the right space to carry out this work. Data availability All the data generated or used in this study are included in this article. Competing interests The author declares no competing interests. Author contributions TI led the study—from designing the experiments and running the lab work to analyzing the data and writing the manuscript. EKO and AM provided supervision, valuable insights, and thoughtful feedback throughout the research process. CKG wrote the methodology, interpreted data, and edited the manuscript. All other authors reviewed, edited, and approved the final version of the manuscript. Author Affiliations 1 Department of Chemistry Education, University of Education, Winneba. Ghana. 2 Department of Chemistry, University of Cape Coast, Cape Coast. Ghana Funding The authors did not receive support from any organization for the submitted work. References Akram M, Rashid A, Zainab R, et al. Application and research of natural products in modern medical treatment. J Mod Pharmacol Pathol . 2023;2023007. https://doi.org/10.53964/jmpp.2023007 Rankoana SA. Indigenous plant-derived medical applications for primary health care. 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J Sep Sci . 2023;47(1). https://doi.org/10.1002/jssc.202300678 Scheme Scheme 1 and 2 are available in the Supplementary Files section. Additional Declarations The authors declare no competing interests. Supplementary Files scheme1.png Scheme 1: Rationalization of Tridemoph scheme2.png Scheme 2: Rationalization of Tyrosol, acetate GraphicalAbstract.docx Graphical Abstract Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6428166","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":441686303,"identity":"b78e7605-e860-4754-bca8-b4471c992268","order_by":0,"name":"Israel Tefe","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA2klEQVRIie3PMQ/BQBTA8SeVu+XR9ZpG+xWeXCIRPgzLmSRGm06dKlYfwyQ2kkYndkaLiaSTECSEganOJnG/4ab7570HYBg/iD3eDgAPrBnkAu2ECHDGGnoJvBIkvaRoLzdpShcPeXQQp0ndAx7PR5mLiZZ0hkQScTl2+gslAZVaZScMXCRqTkV7LAph3AwEVrITO7HOF6Je5O+2zlUrAcXc+/kNFMhcvSlCsWpEshyhkrVSqCT7dIs/SKzVsev5yOPNeh/WPZvHSWbyLi+eq37BSr/5bRiG8T9uFV5A2ZkJDD0AAAAASUVORK5CYII=","orcid":"https://orcid.org/0009-0002-6979-0613","institution":"University of Education, Winneba","correspondingAuthor":true,"prefix":"","firstName":"Israel","middleName":"","lastName":"Tefe","suffix":""},{"id":441686304,"identity":"e69468ab-baa2-4d76-bb2f-4eb14688ed4d","order_by":1,"name":"Emmanuel Kyame Oppong","email":"","orcid":"","institution":"University of Education, 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Winneba","correspondingAuthor":false,"prefix":"","firstName":"Gideon","middleName":"","lastName":"Akiti","suffix":""}],"badges":[],"createdAt":"2025-04-11 12:07:44","currentVersionCode":1,"declarations":{"humanSubjects":false,"vertebrateSubjects":false,"conflictsOfInterestStatement":false,"humanSubjectEthicalGuidelines":false,"humanSubjectConsent":false,"humanSubjectClinicalTrial":false,"humanSubjectCaseReport":false,"vertebrateSubjectEthicalGuidelines":false},"doi":"10.21203/rs.3.rs-6428166/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-6428166/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":80510878,"identity":"03ae3a4e-5e85-4dfd-8a00-49191a16bc90","added_by":"auto","created_at":"2025-04-14 07:03:38","extension":"png","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":158719,"visible":true,"origin":"","legend":"\u003cp\u003eTLC result of TLTS extract\u003c/p\u003e","description":"","filename":"1.png","url":"https://assets-eu.researchsquare.com/files/rs-6428166/v1/db37f173a0ba9c74dd528376.png"},{"id":80511877,"identity":"422f5aa5-75b7-4a66-9d6b-5cfab469c8c6","added_by":"auto","created_at":"2025-04-14 07:11:38","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":38088,"visible":true,"origin":"","legend":"\u003cp\u003eGC-MS spectrum of Tridemorph\u003c/p\u003e","description":"","filename":"2.png","url":"https://assets-eu.researchsquare.com/files/rs-6428166/v1/de88ec7cf78e6029af4eb333.png"},{"id":80510900,"identity":"d1ec671b-e11e-4323-a91c-781db01a92b5","added_by":"auto","created_at":"2025-04-14 07:03:39","extension":"png","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":30311,"visible":true,"origin":"","legend":"\u003cp\u003eGC-MS spectrum of Tyrosol, acetate\u003c/p\u003e","description":"","filename":"3.png","url":"https://assets-eu.researchsquare.com/files/rs-6428166/v1/53cca16c912372a58bd234b3.png"},{"id":80510882,"identity":"4f3f32fc-a556-480d-b72e-120ec38ff24e","added_by":"auto","created_at":"2025-04-14 07:03:38","extension":"png","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":30907,"visible":true,"origin":"","legend":"\u003cp\u003eZone of Inhibition of TLTS against microbial strains\u003c/p\u003e","description":"","filename":"4.png","url":"https://assets-eu.researchsquare.com/files/rs-6428166/v1/68a4455d2ad02dc4a3157f5b.png"},{"id":80510895,"identity":"5cfb783d-a1af-4aed-b2af-d1bc75a7a6a5","added_by":"auto","created_at":"2025-04-14 07:03:39","extension":"png","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":553549,"visible":true,"origin":"","legend":"\u003cp\u003eZone of inhibition of TLTS and ciprofloxacin against microbial strains.\u003c/p\u003e\n\u003cp\u003e\u003cem\u003eCiprofloxacin (Cipro) was used as a standard at a concentration of 1 mg/mL, extract various concentrations: \u003c/em\u003e\u003cem\u003e\u003cstrong\u003eA\u003c/strong\u003e\u003c/em\u003e\u003cem\u003e (1mg/mL), \u003c/em\u003e\u003cem\u003e\u003cstrong\u003eB\u003c/strong\u003e\u003c/em\u003e\u003cem\u003e (0.8 mg/mL), \u003c/em\u003e\u003cem\u003e\u003cstrong\u003eC\u003c/strong\u003e\u003c/em\u003e\u003cem\u003e (0.6 mg/mL) against the microbial strains (E. faecalis, S. pyogenes, S. aureus, K. pneumonae and C. albicans).\u003c/em\u003e\u003c/p\u003e","description":"","filename":"5.png","url":"https://assets-eu.researchsquare.com/files/rs-6428166/v1/b71f3d4fbfe50c86af7f996d.png"},{"id":80513228,"identity":"8bb25192-707f-40f7-9636-a269bc308f80","added_by":"auto","created_at":"2025-04-14 07:27:39","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":2152308,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-6428166/v1/a84549b2-9098-4fd3-8c42-60296fee91a0.pdf"},{"id":80510875,"identity":"e712c200-a7ba-4c2f-918f-f1e0d18ca382","added_by":"auto","created_at":"2025-04-14 07:03:38","extension":"png","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":49572,"visible":true,"origin":"","legend":"\u003cp\u003eScheme 1: Rationalization of Tridemoph\u003c/p\u003e","description":"","filename":"scheme1.png","url":"https://assets-eu.researchsquare.com/files/rs-6428166/v1/8305aa17e10ac344e2efdd52.png"},{"id":80510876,"identity":"ec79a168-97f2-46a2-8443-5014beb7c9b7","added_by":"auto","created_at":"2025-04-14 07:03:38","extension":"png","order_by":2,"title":"","display":"","copyAsset":false,"role":"supplement","size":37706,"visible":true,"origin":"","legend":"\u003cp\u003eScheme 2: Rationalization of Tyrosol, acetate\u003c/p\u003e","description":"","filename":"scheme2.png","url":"https://assets-eu.researchsquare.com/files/rs-6428166/v1/40658145c816535deec60e31.png"},{"id":80510903,"identity":"d1173080-af53-4d9c-accc-deec5063e5eb","added_by":"auto","created_at":"2025-04-14 07:03:39","extension":"docx","order_by":3,"title":"","display":"","copyAsset":false,"role":"supplement","size":292984,"visible":true,"origin":"","legend":"\u003cp\u003e\u003cstrong\u003eGraphical Abstract\u003c/strong\u003e\u003c/p\u003e","description":"","filename":"GraphicalAbstract.docx","url":"https://assets-eu.researchsquare.com/files/rs-6428166/v1/34c2c2b14c894b21da732db4.docx"}],"financialInterests":"The authors declare no competing interests.","formattedTitle":"\u003cp\u003eBioactivity and GC-MS Profile of Extract from Static Cultures of Fungi Isolated from the Leaf of \u003cem\u003eSolanum lycopersicum\u003c/em\u003e\u003c/p\u003e","fulltext":[{"header":"Highlights","content":"\u003cul type=\"disc\"\u003e\n \u003cli\u003eFungi living inside tomato leaves were grown in static cultures to explore their potential.\u003c/li\u003e\n \u003cli\u003eGC-MS analysis uncovered several useful compounds, including Norgestrel and Tyrosol acetate.\u003c/li\u003e\n \u003cli\u003eThe fungal extract successfully fought off harmful bacteria and yeast, showing strong antimicrobial effects.\u003c/li\u003e\n \u003cli\u003eIt was especially effective against \u003cem\u003eE. faecalis\u003c/em\u003e, with a low MIC/MBC of 0.8 mg/mL.\u003c/li\u003e\n \u003cli\u003eThe extract also showed antioxidant activity (34.9%), and TLC revealed a mix of bioactive plant-like compounds.\u003c/li\u003e\n\u003c/ul\u003e"},{"header":"1.0 Introduction","content":"\u003cp\u003eThe role natural products play in modern biotechnology cannot be overlooked as they contribute to over half of all currently available drugs(\u003cspan class=\"CitationRef\"\u003e1\u003c/span\u003e). Approximately 85% of traditional medicine practices rely on plant extracts, and about 80% of individuals in developing countries depend on these remedies for their primary healthcare needs(\u003cspan class=\"CitationRef\"\u003e2\u003c/span\u003e). Additionally, natural sources have played a significant role in cancer treatment, with 62% of the 87% of anticancer drugs approved in the past decade being derived from natural compounds. Notable examples include paclitaxel (\u003cstrong\u003eI\u003c/strong\u003e), from \u003cem\u003eTaxus brevifolia\u003c/em\u003e, and vinblastine, obtained from \u003cem\u003eCatharanthus roseus\u003c/em\u003e (\u003cspan class=\"CitationRef\"\u003e3\u003c/span\u003e). Organic compounds isolated from organisms have proved to be a rich reservoir of novel bioactive molecules with pharmacological potential(\u003cspan class=\"CitationRef\"\u003e4\u003c/span\u003e). This continuous exploration of natural compounds has led to the discovery of impactful medications such as penicillin (\u003cstrong\u003eII)\u003c/strong\u003e, vincristine, and artemisinin, all of which have made substantial contributions to human health and well-being(\u003cspan class=\"CitationRef\"\u003e5\u003c/span\u003e).\u003c/p\u003e\n\u003cp\u003e\u003cimg 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\" width=\"1112\" height=\"431\"\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp\u003eNatural compounds combat bacterial resistance mechanisms, such as biofilm formation, which can be disrupted by certain flavonoids. Some of these natural compounds possess distinct structures and mechanisms of action that set them apart from traditional antibiotics. For instance, magainin, antimicrobial peptides found in amphibian skin, can disrupt bacterial cell membranes by forming pores, ultimately leading to cell lysis (\u003cspan class=\"CitationRef\"\u003e6\u003c/span\u003e). In addition to their unique properties, natural compounds offer a cost-effective alternative to synthetic drugs. By utilizing abundant resources in nature, such as plants, fungi, and microorganisms, researchers can harness therapeutic compounds without relying heavily on expensive synthetic processes. This natural abundance reduces production costs, making drug development more economical compared to synthetic alternatives, which often require complex synthesis and lengthy validation phases (\u003cspan class=\"CitationRef\"\u003e7\u003c/span\u003e)(\u003cspan class=\"CitationRef\"\u003e8\u003c/span\u003e). Natural products have proven to be a valuable source of novel compounds with distinct structures and bioactivities that are not commonly found in synthetic drugs. For example, paclitaxel, a potent chemotherapy agent derived from the Pacific yew plant, has been widely employed in cancer treatment(\u003cspan class=\"CitationRef\"\u003e9\u003c/span\u003e). Similarly, penicillin, discovered from the \u003cem\u003ePenicillium notatum\u003c/em\u003e mold, revolutionized antibiotic therapy and transformed modern medicine. These examples highlight how nature continues to inspire innovative pharmacological breakthroughs and expand opportunities for drug discovery(\u003cspan class=\"CitationRef\"\u003e10\u003c/span\u003e).\u003c/p\u003e\n\u003cp\u003eThis research explores the bioactivity potential derived from tomato plant leaves, with a particular focus on their antimicrobial and antioxidant properties (\u003cspan class=\"CitationRef\"\u003e11\u003c/span\u003e). The bioactive compounds present in the fungal extracts were extracted using ethyl acetate and then subjected to TLC and GC-MS analysis (\u003cspan class=\"CitationRef\"\u003e12\u003c/span\u003e). Additionally, the research determined the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values of the extract against five microbial strains.\u003c/p\u003e"},{"header":"2.0 Methodology","content":"\u003cp\u003eThe study collected samples from Tsito Sec. Tech. An area in the Volta Region, Ghana, used a procedure involving a 3% malt extract and 6% glucose-enriched media broth to create a suitable environment for fungi proliferation. After three weeks of sub-culturing, a specific colony was carefully extracted using sterilized forceps and an inoculation loop and transferred into a sterile 2 dm\u003csup\u003e3\u003c/sup\u003e flat-bottomed flask containing a medium consisting of 3% malt extract and 6% glucose (\u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e13\u003c/span\u003e). The fungal cultures were harvested by filtering the mycelia from the broth using a muslin sieve after eight weeks. The extract was extracted using ethyl acetate, after which the organic layer was subjected to concentration using a rotary evaporator(\u003cspan citationid=\"CR14\" class=\"CitationRef\"\u003e14\u003c/span\u003e).\u003c/p\u003e \u003cp\u003eA solvent system was prepared in a 25:25:3 ratio and poured into a TLC chamber and a small portion of the TLTS extract was dissolved in ethyl acetate and spotted on a TLC plate to dry. A \u003cem\u003ep\u003c/em\u003e-anisaldehyde spray reagent was prepared and sprayed on the plates, heated at 105\u0026deg;C for 15 minutes and visualized. The TLC plates was observed to have pink and deep blue colours at different distances from the baseline (\u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e).\u003c/p\u003e \u003cp\u003eThe GC-MS analysis was conducted using a Thermo Scientific GC TRACE 1300 gas chromatograph paired with an ISQ QD Mass Spectrometer. Its data interpreted using the National Institute of Standards and Technology (NIST) database. The fungal extract tested against \u003cem\u003eEnterococcus faecalis\u003c/em\u003e, \u003cem\u003eStaphylococcus aureus\u003c/em\u003e, \u003cem\u003eKlebsiella pneumoniae, Streptococcus pyogenes\u003c/em\u003e and \u003cem\u003eCandida albicans\u003c/em\u003e for their antifungal and antibacterial effects. Antimicrobial activity was assessed using the agar well diffusion method, following established standardized protocols (\u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e). The MIC of the fungal extract was determined using the Triphenyl Tetrazolium Chloride (TTC)-MIC assay. In this assay, a pink colour indicated bacterial growth, while no colour changes signified successful inhibition of bacterial growth (\u003cspan citationid=\"CR17\" class=\"CitationRef\"\u003e17\u003c/span\u003e). The DPPH (1,1-diphenyl-2-picrylhydrazyl) assay was employed to assess the antioxidant activity of the TLTS extract using the protocol of (Drawell, DNM-9602) (\u003cspan citationid=\"CR18\" class=\"CitationRef\"\u003e18\u003c/span\u003e).\u003c/p\u003e"},{"header":"3.0 Results and Discussions","content":"\u003cdiv id=\"Sec4\" class=\"Section2\"\u003e\n \u003ch2\u003e3.1 Culturing and TLC Analysis\u003c/h2\u003e\n \u003cp\u003e\u003cem\u003eSolanum lycopersicum\u003c/em\u003e leaves were pre-treated with 70% alcohol and sub-cultured for three weeks. After two weeks, TLTS1 showed white mycelia growth, while other sub-cultures had black with white patches. White mycelia growth is useful in fungal culture studies, indicating fungal species and potential biocontrol agents(\u003cspan class=\"CitationRef\"\u003e19\u003c/span\u003e). A solvent system of Hexane: Ethyl acetate: Acetic acid (25:25:3) was used as a mobile phase, which revealed 2 spots with Rf values of 0.50 (spot A) and 0.8 (spot B) as shown in the figure below. The TLC chromatogram showed two distinct colours (pink and deep blue) with \u003cem\u003ep\u003c/em\u003e-Anisaldehyde visualizing reagent. The presence of these colours (pink and blue) showed the presence of alkaloids and phenolic compounds, respectively (\u003cspan class=\"CitationRef\"\u003e20\u003c/span\u003e).\u003c/p\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec5\" class=\"Section2\"\u003e\n \u003ch2\u003e3.2 GC-MS Analysis\u003c/h2\u003e\n \u003cp\u003eA total of 18 most probable compounds were identified in the TLTS extract using GC-MS. This indicates that TLTS extract contains various groups of compounds. Table \u003cspan class=\"InternalRef\"\u003e1\u003c/span\u003e includes the name, retention time, and the mass-to-charge ratio of the compounds.\u003c/p\u003e\n \u003ctable id=\"Tab1\" border=\"1\"\u003e\n \u003ccaption language=\"En\"\u003e\n \u003cdiv class=\"CaptionNumber\"\u003eTable 1\u003c/div\u003e\n \u003cdiv class=\"CaptionContent\"\u003e\n \u003cp\u003eCompounds that are found in TLTS extract by GC-MS\u003c/p\u003e\n \u003c/div\u003e\n \u003c/caption\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eS/N\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eName Of Compound\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eR. T\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eM. F\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eM/Z\u003c/p\u003e\n \u003c/th\u003e\n \u003c/tr\u003e\n \u003c/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eHexanoic acid, 2-phenylethyl ester\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e6.00\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC\u003csub\u003e14\u003c/sub\u003eH\u003csub\u003e20\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e220\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eBenzeneethanol, 4-hydroxy-\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e8.43\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC\u003csub\u003e8\u003c/sub\u003eH\u003csub\u003e10\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e138\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2,4-Hexadienedioic acid\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e9.18\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC\u003csub\u003e6\u003c/sub\u003eH\u003csub\u003e6\u003c/sub\u003eO\u003csub\u003e4\u003c/sub\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e142\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2-Furancarboxylic acid, 5-(hydroxymethyl)-\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e9.36\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC\u003csub\u003e6\u003c/sub\u003eH\u003csub\u003e6\u003c/sub\u003eO\u003csub\u003e4\u003c/sub\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e142\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eBenzoic acid, 3-hydroxy-\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e9.91\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC\u003csub\u003e7\u003c/sub\u003eH\u003csub\u003e6\u003c/sub\u003eO\u003csub\u003e3\u003c/sub\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e138\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eTyrosol, acetate\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e10. 04\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC\u003csub\u003e10\u003c/sub\u003eH\u003csub\u003e12\u003c/sub\u003eO\u003csub\u003e3\u003c/sub\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e180\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eBisabolol oxide B\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e14. 42\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC\u003csub\u003e15\u003c/sub\u003eH\u003csub\u003e26\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e238\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eHexadecanoic acid, ethyl ester\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e15. 14\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC\u003csub\u003e18\u003c/sub\u003eH\u003csub\u003e36\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e284\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eLinoelaidic acid\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e17. 26\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC\u003csub\u003e18\u003c/sub\u003eH\u003csub\u003e32\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e280\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e10\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eOctadecanoic acid\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e17. 64\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC\u003csub\u003e18\u003c/sub\u003eH\u003csub\u003e36\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e284\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e11\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eN,N\u0026apos;-Bis(Carbobenzylox y)-lysine methyl(ester)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e19. 48\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC\u003csub\u003e23\u003c/sub\u003eH\u003csub\u003e28\u003c/sub\u003eN\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e6\u003c/sub\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e428\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e12\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eSpiculesporic acid\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e21. 54\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC\u003csub\u003e17\u003c/sub\u003eH\u003csub\u003e28\u003c/sub\u003eO\u003csub\u003e6\u003c/sub\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e328\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e13\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e(9Z,12Z)-Phenethyl octadeca-9,12-dienoate\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e27. 74\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC\u003csub\u003e26\u003c/sub\u003eH\u003csub\u003e40\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e384\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e14\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eSolasonine\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e8.02\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC\u003csub\u003e45\u003c/sub\u003eH\u003csub\u003e73\u003c/sub\u003eNO\u003csub\u003e16\u003c/sub\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e883\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e15\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eTridemorph\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e11. 14\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC\u003csub\u003e19\u003c/sub\u003eH\u003csub\u003e39\u003c/sub\u003eNO\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e297\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e16\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNorgestrel\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e17. 54\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC\u003csub\u003e21\u003c/sub\u003eH\u003csub\u003e28\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e312\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e17\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eDoconexent\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e17. 54\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC\u003csub\u003e22\u003c/sub\u003eH\u003csub\u003e32\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e328\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e18\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eSantamarine\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e17. 95\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eC\u003csub\u003e15\u003c/sub\u003eH\u003csub\u003e20\u003c/sub\u003eO\u003csub\u003e3\u003c/sub\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e248\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n \u003c/table\u003e\n \u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n \u003ctable id=\"Tab2\" border=\"1\"\u003e\n \u003ccaption language=\"En\"\u003e\n \u003cdiv class=\"CaptionNumber\"\u003eTable 2\u003c/div\u003e\n \u003cdiv class=\"CaptionContent\"\u003e\n \u003cp\u003eAntimicrobial activity of compounds found in TLTS extract\u003c/p\u003e\n \u003c/div\u003e\n \u003c/caption\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eS/N\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eName of Compound\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eAntimicrobial activity\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eReference\u003c/p\u003e\n \u003c/th\u003e\n \u003c/tr\u003e\n \u003c/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eHexanoic acid, 2-phenylethyl ester\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eAntibacterial\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e(\u003cspan class=\"CitationRef\"\u003e21\u003c/span\u003e)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eBenzeneethanol, 4-hydroxy-\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eAntioxidant\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e(\u003cspan class=\"CitationRef\"\u003e22\u003c/span\u003e)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2,4-Hexadienedioic acid\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eAntimicrobial\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e(\u003cspan class=\"CitationRef\"\u003e23\u003c/span\u003e)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2-Furancarboxylic acid, 5-(hydroxymethyl)-\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eantioxidant\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e(\u003cspan class=\"CitationRef\"\u003e24\u003c/span\u003e)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eBenzoic acid, 3-hydroxy-\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eAntioxidant\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e(\u003cspan class=\"CitationRef\"\u003e25\u003c/span\u003e)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eTyrosol, acetate\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eAntioxidant\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e(\u003cspan class=\"CitationRef\"\u003e26\u003c/span\u003e)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eBisabolol oxide B\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eAntimicrobial\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e(\u003cspan class=\"CitationRef\"\u003e27\u003c/span\u003e)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eHexadecanoic acid, ethyl ester\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eAntimicrobial\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e(\u003cspan class=\"CitationRef\"\u003e28\u003c/span\u003e)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eLinoelaidic acid\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eAntimicrobial\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e(\u003cspan class=\"CitationRef\"\u003e29\u003c/span\u003e)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e10\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eOctadecanoic acid\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eAntimicrobial\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e(\u003cspan class=\"CitationRef\"\u003e30\u003c/span\u003e)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e11\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eN, N\u0026apos;-Bis (Carbobenzylox y)-lysine methyl(ester)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eAntimicrobial\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e(\u003cspan class=\"CitationRef\"\u003e31\u003c/span\u003e)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e12\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eSpiculesporic acid\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eAntimicrobial, antifungal\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e(\u003cspan class=\"CitationRef\"\u003e32\u003c/span\u003e)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e13\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e(9Z,12Z)-Phenethyl octadeca-9,12-dienoate\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eAntimicrobial\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e(\u003cspan class=\"CitationRef\"\u003e33\u003c/span\u003e)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e14\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eSolasonine\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eAntimicrobial\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e(\u003cspan class=\"CitationRef\"\u003e34\u003c/span\u003e)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e15\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eTridemorph\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eAntifungal\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e(\u003cspan class=\"CitationRef\"\u003e35\u003c/span\u003e)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e16\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNorgestrel\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eProgestogenic, anti-estrogenic\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e(\u003cspan class=\"CitationRef\"\u003e36\u003c/span\u003e)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e17\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eDoconexent\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eAntioxidant\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e(\u003cspan class=\"CitationRef\"\u003e37\u003c/span\u003e)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e18\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eSantamarine\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eAnticancer\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e(\u003cspan class=\"CitationRef\"\u003e38\u003c/span\u003e)\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n \u003c/table\u003e\n \u003cp\u003eThe eighteen (\u003cspan class=\"CitationRef\"\u003e18\u003c/span\u003e) different compounds identified through GC-MS analysis of the extract of TLTS (Table \u003cspan class=\"InternalRef\"\u003e1\u003c/span\u003e) indicate a rich chemical reservoir of the TLTS extract. Some of the components in TLTS extract have been found to exhibit antimicrobial, antioxidant, antifungal, and anticancer properties, as documented in the literature (Table \u003cspan class=\"InternalRef\"\u003e2\u003c/span\u003e). Fatty acid derivatives such as Hexadecanoic acid (\u003cspan class=\"CitationRef\"\u003e39\u003c/span\u003e) and Octadecanoic acid have demonstrated antimicrobial properties through their membrane disruption abilities, which is shown in the zone of inhibition against the bacterial strains (\u003cspan class=\"CitationRef\"\u003e30\u003c/span\u003e), (Table \u003cspan class=\"InternalRef\"\u003e3\u003c/span\u003e).\u003c/p\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec6\" class=\"Section2\"\u003e\n \u003ch2\u003e3.3 Rationalization of GC-MS Fragmented Compounds\u003c/h2\u003e\n \u003cdiv id=\"Sec7\" class=\"Section3\"\u003e\n \u003ch2\u003e3.3.1 Tridemorph\u003c/h2\u003e\n \u003cp\u003eTridemorph exhibited six (\u003cspan class=\"CitationRef\"\u003e6\u003c/span\u003e) significant peaks at m/z 297, 128, 71, 57, 43, and 29 within the electron impact (EI) mass spectrum. Among these fragment ions, the parent ion peak at m/z 297 was identified. This peak emerged as a result of the ejection of an electron from the molecular ion. The parent ion was detected on the spectrum, signifying the molecular weight of the compound. The pentyl cation at m/z\u0026thinsp;=\u0026thinsp;71 with 5% abundance appeared on the spectrum due to homolytic cleavage of the C-C single bond through route \u003cstrong\u003e(a)\u003c/strong\u003e. This fragment ion further undergoes fragmentation to lose methyl radical as a result of cleavage of the C-C single bond to generate butyl radical cation at m/z\u0026thinsp;=\u0026thinsp;57 with 20% abundance via pathway \u003cstrong\u003e(b)\u003c/strong\u003e. The butyl radical cation eliminated methyl radical through homolytic cleavage at pathway \u003cstrong\u003e(c)\u003c/strong\u003e to produce propyl cation at m/z\u0026thinsp;=\u0026thinsp;43 with an abundance of 48% on the spectrum. Again, the propyl cation also fragments homolytically through route \u003cstrong\u003e(d)\u003c/strong\u003e by liberation of methyl radical to generate ethyl radical cation at m/z\u0026thinsp;=\u0026thinsp;29 with 18% abundance. The base peak at m/z\u0026thinsp;=\u0026thinsp;128 with 100% abundance resulted from the homolytic cleavage of the C-N bond linking the morpholine ring to the aliphatic chain, leading to the formation of the morpholinium radical cation via pathway \u003cstrong\u003e(e)\u003c/strong\u003e. Consequently, the ions identified in the analysis correspond to the expected fragmentation pattern of tridemorph, thereby providing enough evidence for the compound\u0026rsquo;s identification.\u003c/p\u003e\n \u003c/div\u003e\n \u003cdiv id=\"Sec8\" class=\"Section3\"\u003e\n \u003ch2\u003e3.3.2 Tyrosol, acetate\u003c/h2\u003e\n \u003cp\u003eTyrosol acetate exhibited five (\u003cspan class=\"CitationRef\"\u003e5\u003c/span\u003e) prominent peaks at m/z 107, 120, 121, and 180 within the electron impact (EI) mass spectrum. Among these fragment ions, the precursor parent ion peak at m/z 180 was identified. This peak arose as a result of an electron being dislodged from the molecular structure. The parent ion was observed at highest mass that occur at an even value of mass-to charge in the spectrum, confirming the molecular weight of the compound. The loss of the (C\u003csub\u003e3\u003c/sub\u003eH\u003csub\u003e5\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e) radical through pathway \u003cstrong\u003e(a)\u003c/strong\u003e via homolytic cleavage of the ethylene bridge connecting the phenol and the ether moiety, produced p-cresol radical cation at m/z 107 which was prominently displayed at the midpoint of the spectrum with abundance, 45%. Pathway \u003cstrong\u003e(b)\u003c/strong\u003e involved an alpha cleavage of the ether bond, corresponding to the fragment ion observed at m/z 121 in the spectrum with 15% abundance. The base peak at m/z 120 with 100% abundance was displayed in the spectrum due to loss of hydrogen radical from (C\u003csub\u003e2\u003c/sub\u003eH\u003csub\u003e3\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e) ion that resulted in carbocation rearrangement to produce a more stable ion as illustrated in pathway \u003cstrong\u003e(c)\u003c/strong\u003e. This fragment ion serves as a distinguishing feature of Tyrosol acetate, frequently employed as a diagnostic fragment for the identification of the compound. Consequently, the ions observed in the GC-MS analysis align with the anticipated fragmentation pattern of Tyrosol acetate, thereby providing compelling evidence for the identification of the compound as Tyrosol acetate.\u003c/p\u003e\n \u003c/div\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec9\" class=\"Section2\"\u003e\n \u003ch2\u003e3.4 Antimicrobial and antioxidant activity of TLTS extract\u003c/h2\u003e\n \u003cp\u003eFive microbial strains, \u003cem\u003eE. faecalis\u003c/em\u003e, \u003cem\u003eS. pyogenes\u003c/em\u003e, \u003cem\u003eK. pneumoniae\u003c/em\u003e, \u003cem\u003eS. aureus\u003c/em\u003e, and \u003cem\u003eC. albicans\u003c/em\u003e, were selected based on their resistance to antimicrobial agents and disease characteristics. These microbial strains are involved in various clinical diseases such as skin infections, pharyngitis, urinary tract infections, and blood infections (\u003cspan class=\"CitationRef\"\u003e40\u003c/span\u003e).\u003c/p\u003e\n \u003cdiv id=\"Sec10\" class=\"Section3\"\u003e\n \u003ch2\u003e3.4.1 Zone of Inhibition\u003c/h2\u003e\n \u003cp\u003eThe TLTS extract showed strong antimicrobial properties against \u003cem\u003eE. faecalis\u003c/em\u003e, a leading cause of infections in hospitals due to its resistance to some antibiotics. However, the TLTS extract\u0026rsquo;s efficiency varies significantly with the type of pathogen, as different bacteria have distinct cell wall compositions and structures (41). Gram-positive bacteria such as \u003cem\u003eS. aureus\u003c/em\u003e have a robust peptidoglycan layer, making them more vulnerable to certain chemical compounds in the TLTS extract. Gram-negative bacteria such as \u003cem\u003eK. pneumoniae\u003c/em\u003e have an outer membrane that can serve as a barrier to antibacterial compounds. The suppressive impact on \u003cem\u003eC. albicans\u003c/em\u003e by the TLTS extract suggests the potential of the extract as a broad-spectrum treatment, especially in immunocompromised patients with severe Candida infections (\u003cspan class=\"CitationRef\"\u003e42\u003c/span\u003e).\u003c/p\u003e\n \u003c/div\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec11\" class=\"Section2\"\u003e\n \u003ch2\u003e\u003cstrong\u003e3.5 Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC\u003c/strong\u003e)\u003c/h2\u003e\n \u003cp\u003eThe TLTS extract effectively combats pathogens like \u003cem\u003eE. faecalis\u003c/em\u003e, \u003cem\u003eS. pyogenes\u003c/em\u003e, and \u003cem\u003eK. pneumoniae\u003c/em\u003e, with MIC and MBC values of 0.8, making it suitable for clinical use due to lower potential side effects. However, \u003cem\u003eS. aureus\u003c/em\u003e and \u003cem\u003eC. albicans\u003c/em\u003e show more resistance, necessitating further study(\u003cspan class=\"CitationRef\"\u003e43\u003c/span\u003e). On the other hand, reported MIC and MBC values of 8.0\u0026thinsp;\u0026plusmn;\u0026thinsp;0.18 and 12.0\u0026thinsp;\u0026plusmn;\u0026thinsp;0.17 mg/mL, respectively, against the same microbes. These variations are likely due to differences in extraction and testing methods.\u003c/p\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec12\" class=\"Section2\"\u003e\n \u003ch2\u003e3.6 The Free Radical Scavenging Activity of TLTS Extracts\u003c/h2\u003e\n \u003cp\u003eThis research also assessed the antioxidant activity of TLTS extract using the DPPH assay, which revealed varying levels of activity with a maximum inhibition percentage of 34.9%. The lowest absorbance (0.829) indicates the most substantial inhibition, suggesting that the extract effectively reduces DPPH radicals. In contrast, the highest absorbance (0.989) corresponds to a lower inhibition of 22.3%, pointing to different antioxidant concentrations within the extract. This variability suggests that not all components share the same antioxidant properties. The extract\u0026apos;s 34.9% inhibition indicates its potential as a functional food. Additionally, compounds such as 2,4-Hexadienedioic acid and Bisabolol oxide B are noted for their antimicrobial and antifungal effects, which support the development of multi-target therapies (\u003cspan class=\"CitationRef\"\u003e44\u003c/span\u003e).\u003c/p\u003e\n \u003ctable id=\"Tab3\" border=\"1\"\u003e\n \u003ccaption language=\"En\"\u003e\n \u003cdiv class=\"CaptionNumber\"\u003eTable 3\u003c/div\u003e\n \u003cdiv class=\"CaptionContent\"\u003e\n \u003cp\u003eMicrobial activity and Antioxidant effectiveness of TLTS Extract\u003c/p\u003e\n \u003c/div\u003e\n \u003c/caption\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth align=\"left\" colspan=\"2\"\u003e\n \u003cp\u003eZone of Inhibition\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" rowspan=\"2\"\u003e\n \u003cp\u003eMIC\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" rowspan=\"2\"\u003e\n \u003cp\u003eMBC\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" colspan=\"3\"\u003e\n \u003cp\u003eAntioxidant Activity\u003c/p\u003e\n \u003c/th\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eOrganism\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eTLTS\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eS/N\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eAbso. Extract\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003e%Inhibition\u003c/p\u003e\n \u003c/th\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003e\u003cem\u003eE. faecalis\u003c/em\u003e (NCTC 13379)\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003e13\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003e0.8\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003e0.8\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003e1\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003e0.829\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003e34.9\u003c/p\u003e\n \u003c/th\u003e\n \u003c/tr\u003e\n \u003c/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cem\u003eS.pyogenes\u003c/em\u003e (NCTC 12696)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e7\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.929\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e27.0\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cem\u003eK.pneumonae\u003c/em\u003e (NCTC 13348)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e6\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.8\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.989\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e22.3\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cem\u003eS. aureus\u003c/em\u003e (NCTC 12973)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e4\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.924\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e27.4\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cem\u003eC. albicans\u003c/em\u003e (ATCC90028)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e10\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e5\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0953\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"char\"\u003e\n \u003cp\u003e25.1\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n \u003c/table\u003e\n\u003c/div\u003e"},{"header":"4.0 Conclusion","content":"\u003cp\u003eThis research extracted bioactive compounds from endophytic fungi of \u003cem\u003eSolanum lycopersicum\u003c/em\u003e leaves and identified them using GC-MS. A diverse range of secondary metabolites, including Norgestrel, Tyrosol, Bisabolol oxide B, Doconexent, and Santamarine, were among the major compounds produced. The TLTS extract exhibited significant antimicrobial effects, particularly against \u003cem\u003eEnterococcus faecalis\u003c/em\u003e. These results not only emphasise the potential of endophytic fungi as a promising source of therapeutic agents, especially in the context of rising antibiotic resistance, but also open avenues for their applications in pharmaceuticals. Future research should focus on elucidating these compounds' mechanisms of action, evaluating their efficacy in vivo, and considering the ecological importance of endophytic fungi in promoting plant health and sustainability.\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eAcknowledgements\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eI\u0026apos;m truly grateful to Prof. Emmanuel Kyame Oppong for his guidance and support. Thanks also to the Department of Chemistry, University of Education, Winneba, for creating the right space to carry out this work.\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eData availability\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAll the data generated or used in this study are included in this article.\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompeting interests\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe author declares no competing interests.\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAuthor contributions\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eTI led the study\u0026mdash;from designing the experiments and running the lab work to analyzing the data and writing the manuscript. EKO and AM provided supervision, valuable insights, and thoughtful feedback throughout the research process. CKG wrote the methodology, interpreted data, and edited the manuscript.\u0026nbsp;All other authors reviewed, edited, and approved the final version of the manuscript.\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAuthor Affiliations\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003csup\u003e1\u003c/sup\u003e Department of Chemistry Education, University of Education, Winneba. Ghana.\u003c/p\u003e\n\u003cp\u003e\u003csup\u003e2\u003c/sup\u003eDepartment of Chemistry, University of Cape Coast, Cape Coast. Ghana\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFunding\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe authors did not receive support from any organization for the submitted work.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\n \u003cli\u003eAkram M, Rashid A, Zainab R, et al. 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Extractions of aerial parts of \u003cem\u003eHippomarathrum scabrum\u003c/em\u003e with conventional and green methodologies: chemical profiling, antioxidant, enzyme inhibition, and anti-cancer effects. \u003cem\u003eJ Sep Sci\u003c/em\u003e. 2023;47(1). https://doi.org/10.1002/jssc.202300678\u003c/li\u003e\n\u003c/ol\u003e"},{"header":"Scheme ","content":"\u003cp\u003eScheme 1 and 2 are available in the Supplementary Files section.\u003c/p\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"University of Education, Winneba","isAcceptedByJournal":false,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Rotary Evaporator, Solanum lycopersicum, GC-MS, Agar well diffusion, Thin-layer chromatography","lastPublishedDoi":"10.21203/rs.3.rs-6428166/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-6428166/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eThis research investigated bioactive compounds produced by endophytic fungi isolated from \u003cem\u003eSolanum lycopersicum\u003c/em\u003e (tomato) leaves, with a focus on antimicrobial and antioxidant properties. The fungal extract exhibited significant antimicrobial activity against \u003cem\u003eStaphylococcus aureus\u003c/em\u003e, \u003cem\u003eStreptococcus pyogenes\u003c/em\u003e, \u003cem\u003eEnterococcus faecalis\u003c/em\u003e, \u003cem\u003eKlebsiella\u003c/em\u003e \u003cem\u003epneumoniae\u003c/em\u003e and \u003cem\u003eCandida albicans\u003c/em\u003e. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values were 0.8 mg/mL, demonstrating the extract's potency, particularly against \u003cem\u003eE. faecalis\u003c/em\u003e. The extract also showed antioxidant activity, with a 34.9% inhibition rate. TLC analysis, using a hexane: ethyl acetate: acetic acid (25:25:3) solvent system, identified two distinct spots (spots A and B) with Rf values of 0.50 and 0.80, respectively. Visualisation with \u003cem\u003ep\u003c/em\u003e-Anisaldehyde revealed pink and deep blue colours, confirming the presence of various bioactive compounds. This research highlights the potential of endophytic fungi as valuable sources of antimicrobial and antioxidant agents, with significant implications for pharmaceuticals and public health.\u003c/p\u003e","manuscriptTitle":"Bioactivity and GC-MS Profile of Extract from Static Cultures of Fungi Isolated from the Leaf of Solanum lycopersicum","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-04-14 07:03:33","doi":"10.21203/rs.3.rs-6428166/v1","editorialEvents":[{"type":"communityComments","content":1}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"087cddb0-18dd-4ad5-948a-458e56d3f5e8","owner":[],"postedDate":"April 14th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":47107439,"name":"Natural Product Chemistry"}],"tags":[],"updatedAt":"2025-04-14T07:03:33+00:00","versionOfRecord":[],"versionCreatedAt":"2025-04-14 07:03:33","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-6428166","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-6428166","identity":"rs-6428166","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}