Screening for anti-neoplastic enzymes producing halophilic bacterial extract and their antioxidant activity due to carotenoid synthesis

Out of 1127 halophilic bacteria, salt-loving microorganisms from different geographical locations in India isolated, twenty-eight strains were studied for antioxidant qualities for its usage in cancer medicines. The methanolic extracts were extracted and analyzed for anti-cancer effect through anti-neoplastic enzymes (L-asparaginase and L-glutaminase) screening, while antioxidant capabilities were investigated for fifteen isolates using both radical and non-radical assays.. The extracts also have enhanced radical scavenging ability against the stable 2,2'azinobis (3-ethylbenzothiazoline-6-sulfonic acid) disodium salt radical cation (86.34 ± 0.007 mM TE/g extract), nitric oxide (64.79 ± 0.004 µg/mL), phosphomolybdenum (1.69 ± 0.024 mg AAE/g extract), ferric reducing power (28.19 ± 0.012 mM Fe (II) E/mg) and showed better Ic 50 values on 1,1-diphenyl- 2-picrylhydrazyl (5.48 mg/ml) assay. All of the studies has been done three times, and the results are significantly better than the standards utilized. Finally, four potent halophilic isolates from various antioxidant activities were chosen. These isolates could synthesize carotenoids that possessed anti-oxidant activities when grown in the halophilic medium was identified by UV/Visible spectra analysis. Secondary metabolite investigations using GC-MS study and LC-MS/MS analysis confirmed that isolated strains had the potential to produce compounds that might be used primarily as therapeutics. The enormous anti-oxidant potential of the strains Staphylococcus arlettae , Bacillus subtilis , B . tequilensis , and B . haynesii extracts could be used as a natural free radical scavenger to prevent oxidative-stress-related illnesses like cancer, neurodegeneration, as well as in the food safety markets in general.